海鱼致病菌鳗弧菌毒力质粒DNA序列分析。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01

Hai-Zhen Wu, Hui-Zhan Zhang, Cai-Xia Lü, Na Liang, Hui-Yi Jin, Yue Ma, Yuan-Xing Zhang

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引用次数: 0

摘要

采用限制性内切酶切法、亚克隆法和引物步移法，测定了海鱼致病菌鳗弧菌毒力相关质粒pEIB1的DNA序列。获得的pEIB1 DNA序列全长为66 164 bp，总G+C含量为42.7%。该序列编码44个开放阅读框，包含DNA复制、铁载体anguibactin的生物合成和调控以及铁-anguibactin复合物运输的基因。

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DNA sequencing of a plasmid with virulence from marine fish pathogen Vibrio anguillarum.

DNA sequence of a plasmid pEIB1 associated with virulence from the marine fish pathogen Vibrio anguillarum was determined using the methods of restriction endonuclease digestion, subcloning, and primer walking. The whole length of obtained pEIB1 DNA sequence was 66 164 bp, and the overall G+C content of DNA sequence is 42.7%. This sequence encodes 44 open reading frames containing the genes of DNA replication, biosynthesis and regulation of the siderophore anguibactin and transport of ferric-anguibactin complexes.

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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

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