Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica最新文献

[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx]. N-LMP1转基因小鼠鼻咽特异性调控区构建

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Ling Zhang, Ke Lan, Xiang-Ling Feng, Gui-Lin Qiao, Xin-Ming Shen, Yi-Min Shi, Hong Li, Kai-Tai Yao

引用次数: 0

Long term gene therapy of Parkinson's disease using immortalized rat glial cell line with tyrosine hydroxylase gene. 用带酪氨酸羟化酶基因的永生化大鼠神经胶质细胞系长期治疗帕金森病。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Ming Zhuo, De-Hua Xu, Lei Cao, Ling-Fei Xu, Fu-Rong Yu, Zhong-Cheng Zheng, Xin-Yuan Liu

{"title":"Long term gene therapy of Parkinson's disease using immortalized rat glial cell line with tyrosine hydroxylase gene.","authors":"Ming Zhuo, De-Hua Xu, Lei Cao, Ling-Fei Xu, Fu-Rong Yu, Zhong-Cheng Zheng, Xin-Yuan Liu","doi":"","DOIUrl":"","url":null,"abstract":"Glial cell is an ideal vehicle for gene therapy of brain diseases. However, there are many limits in using primary glial cells. Therefore, an immortalized rat glial cell line (RGLT) was established by SV40 large T-antigen (LTag) gene from the primary rat fetal glial cells. The RGLT cell was shown to be non-tumorigenic after transplantation to nude mice (up to 4 weeks) and rat striatum (up to 18 months). Rat tyrosine hydroxylase (TH) gene was transfected into RGLT cell to obtain RGLT-TH cell. The TH immunohistochemical staining and HPLC-ECD analysis demonstrated the TH expression and dopamine (DA) production in RGLT-TH cells in vitro. When implanting RGLT-TH cells into the striatum of 6-hydroxydopamine (6-OHDA) lesioned hemiparkinsonism model rats, TH immunohistochemical staining showed the TH presence in striatum and HPLC-ECD analysis held at 6 months after cell implantation showed an increase of DA content in striatum. The asymmetric rotation of rats receiving RGLT-TH cells was reduced by 50%-60% and this reduction persisted stably at least for 18 months. These results suggest that the immortalized glial cell line could serve as an ideal vehicle for therapeutic gene delivery system to achieve a long-term gene therapy of neurodegenerative diseases.","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effects of sodium selenite on telomerase activity and telomere length]. 亚硒酸钠对端粒酶活性和端粒长度的影响。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Qiong Liu, Hong Wang, De-Cong Hu, Chao-Jian Ding, Heng Xiao, Hui-Bi Xu, Bai-Hua Shu, Shun-Qing Xu

{"title":"[Effects of sodium selenite on telomerase activity and telomere length].","authors":"Qiong Liu, Hong Wang, De-Cong Hu, Chao-Jian Ding, Heng Xiao, Hui-Bi Xu, Bai-Hua Shu, Shun-Qing Xu","doi":"","DOIUrl":"","url":null,"abstract":"To study the biological basis of selenium in resisting senescence through its effects on cellular telomerase activity and telomere length. In the experiments, the cell line of hepatocytes L-02 was divided into three groups supplemented with sodium selenite at final concentrations of 0, 0.5 and 2.5 micromol/L, respectively. Cellular telomerase activity was measured by telomeric repeat amplification protocol and enzymatic luminometric inorganic pyrophosphate detection assay. RT-PCR was used to semi-quantitatively detect human telomerase reverse transcriptase (hTERT) gene expression. The change of telomere length was assayed through flow cytometry and fluorescence in situ hybridization. Results showed that L-02 cells had low telomerase activity and hTERT gene expression level when cultured in the normal way. The cells grew well after 3-week-cultivation in the media supplemented with 0.5 or 2.5 micromol/L sodium selenite. Besides, sodium selenite significantly increased cellular telomerase activity and hTERT gene expression level. The telomere length of L-02 cells was also extended after 4-week-cultivation with sodium selenite. Thus, sodium selenite at nutritional doses could prolong the life span of hepatocytes L-02 through increasing telomerase activity and telomere length. This result provides a possible mechanism for explaining the anti-senescence function of selenium.","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effects of site-directed mutagenesis at amino acid residues of GATA-1b different from that of GATA-1a in Xenopus]. [对爪蟾GATA-1b与GATA-1a不同氨基酸残基的定点诱变效应]。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Xiang-Ling Feng, Wei-Dong Liu, Hong Li, Lei Wang, Xu-Yu Yang, Wei-Nong Han, Wen Zhou, Kai-Tai Yao

{"title":"[Effects of site-directed mutagenesis at amino acid residues of GATA-1b different from that of GATA-1a in Xenopus].","authors":"Xiang-Ling Feng, Wei-Dong Liu, Hong Li, Lei Wang, Xu-Yu Yang, Wei-Nong Han, Wen Zhou, Kai-Tai Yao","doi":"","DOIUrl":"","url":null,"abstract":"The GATA-1 of Xenopus (xGATA-1), which has two subtypes xGATA-1a and xGATA-1b, is a necessary factor for erythroid differentiation and maturation as similar as that of other GATA-1s. Although both xGATa-1a and xGATA-1b are able to stimulate erythropoiesis, only xGATA-1b is capable of inhibiting neurogenesis in Xenopus embryos. Compared between their structures, xGATA-1a and xGATA-1b are very similar in nucleotide and amino acids composition, but not identical. Therefore, it is responsible for studying the role of the diverse codons between the two genes, so the desired mutations: S(168), H(169) double deletion and point mutation of T(304)-->A, T(359)-->A, were introduced into xGATA-1b gene through site-directed mutagenesis. Then, mRNA from each mutant as well as wtxGATA-1b was co-injected with DN-BR mRNA or separately injected into Xenopus stage 2 embryos, and the role of mutants in erythropoiesis and neurogenesis was analyzed by using animal cap culture system. The results showed that the neural-inhibiting activity of xGATA-1b, but not hematopoiesis-inducing activity, was aborted because of deletion of Ser(168) and His(169) or point mutation of T(359)-->A. So it is demonstrated for the first time that Ser(168) and His(169) or Thr(359)in xGATA-1b may be one of the structural basis for explanting the different function between xGATA-1b and xGATA-1a.","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon. 干扰素诱导乙型肝炎病毒转染细胞株基因表达分析。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Wei Xiong, Xun Wang, Xiao-Ying Liu, Li Xiang, Ling-Jie Zheng, Jiang-Xia Liu, Zheng-Hong Yuan

{"title":"Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon.","authors":"Wei Xiong, Xun Wang, Xiao-Ying Liu, Li Xiang, Ling-Jie Zheng, Jiang-Xia Liu, Zheng-Hong Yuan","doi":"","DOIUrl":"","url":null,"abstract":"Infection of hepatitis B virus (HBV) continues to be a significant health problem. alpha interferon (IFN-alpha) and gamma interferon (IFN-gamma) have been proven to be effective in inhibiting HBV replication. To study the global effect of HBV persistent existence on IFN induced cellular gene expression, cDNA microarrays dotted with 14 112 human genes were used to examine the transcriptional changes between an HBV DNA transfected cell line (HepG2.2.15) and its parental cell line (HepG2) after the treatment of IFN-alpha or IFN-gamma for 6 h. The results showed that many genes related to cell cycle, proliferation, apoptosis and new ESTs were regulated by IFN-alpha and/or IFN-gamma. Many genes involved in kinase and signal transduction, transcription regulation, antigen presentation and processing were differentially regulated between these two cell lines post IFN-alpha or IFN-gamma treatment. Interestingly, several IFN-differentially regulated genes, such as MyD88 and Diubiquitin, were found to inhibit HBV gene expression, and MyD88 was proved to inhibit HBV replication. Taken together, our results revealed the global effects of HBV persistent existence on IFN-induced cellular gene expression. The novel antiviral genes identified by microarray could be potentially developed as new anti-HBV drugs or for novel therapies.","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24122332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma japonicum gene]. 日本血吸虫新基因sjf4的克隆、表达及功能分析

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Liang Zhang, Guo-Feng Cheng, Zhi-Qiang Fu, Ya-Mei Jin, Xin-Gang Feng, Chun-Xiu Yuan, You-Min Cai, Jiao-Jiao Lin

引用次数: 0

[Purification of a big defensin from Ruditapes philippinesis and its antibacterial activity]. [菲律宾鲁贝大防御素的纯化及其抑菌活性]。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Yu-Xi Wei, Dao-Sen Guo, Rong-Gui Li, Hao-Wen Chen, Pei-Xun Chen

引用次数: 0

Evolution of phenylalanyl-tRNA synthetase by domain losing. 苯基丙烯酰trna合成酶的结构域丢失进化。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Jun Lin, Jing-Fei Huang

引用次数: 0

[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide]. 人B淋巴细胞刺激物c端肽的免疫增强作用。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Rui-Yang Tian, Wei Han, Ying Yu, Yue Chen, Gu-Song Yu, Sheng-Li Yang, Yi Gong

引用次数: 0

[A method for delineation of domains in proteins based on refolding free energy--application to continuous and discontinuous domains]. [一种基于可折叠自由能的蛋白质结构域描述方法——应用于连续和不连续结构域]。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Zhi-Qun Xie, Gen-Jun Xu

{"title":"[A method for delineation of domains in proteins based on refolding free energy--application to continuous and discontinuous domains].","authors":"Zhi-Qun Xie, Gen-Jun Xu","doi":"","DOIUrl":"","url":null,"abstract":"Domain is a protein architecture in a subunit. It might be defined as a basic unit for structure, function, folding, evolution and design. Different combinations of domains lead to the formation of various tertiary structures with various functions for proteins. The delineation of domains for a protein is important both conceptually and practically, which remains up to date a challenging and unsolved problem. Based on the above definition, a method was previously proposed based on refolding free energy to define continuous domains in proteins. By constructing a residue-residue contact matrix, using correspondence analysis, and then selecting optimal partition function of a protein according to refolding free energy and some empirical scoring functions, a new computer program, PDOM, was developed, which was applicable to both continuous and discontinuous domains. When compared with the manual partition results reported by crystallographers, PDOM has achieved an accuracy of 76% on a test data set including 55 protein structures frequently used. The differences in 13 proteins between PDOM, literature as well as SCOP have been discussed extensively.","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0