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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica最新文献

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[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx]. N-LMP1转基因小鼠鼻咽特异性调控区构建
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Ling Zhang, Ke Lan, Xiang-Ling Feng, Gui-Lin Qiao, Xin-Ming Shen, Yi-Min Shi, Hong Li, Kai-Tai Yao
{"title":"[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx].","authors":"Ling Zhang,&nbsp;Ke Lan,&nbsp;Xiang-Ling Feng,&nbsp;Gui-Lin Qiao,&nbsp;Xin-Ming Shen,&nbsp;Yi-Min Shi,&nbsp;Hong Li,&nbsp;Kai-Tai Yao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1072-6"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effects of sodium selenite on telomerase activity and telomere length]. 亚硒酸钠对端粒酶活性和端粒长度的影响。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Qiong Liu, Hong Wang, De-Cong Hu, Chao-Jian Ding, Heng Xiao, Hui-Bi Xu, Bai-Hua Shu, Shun-Qing Xu
{"title":"[Effects of sodium selenite on telomerase activity and telomere length].","authors":"Qiong Liu,&nbsp;Hong Wang,&nbsp;De-Cong Hu,&nbsp;Chao-Jian Ding,&nbsp;Heng Xiao,&nbsp;Hui-Bi Xu,&nbsp;Bai-Hua Shu,&nbsp;Shun-Qing Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To study the biological basis of selenium in resisting senescence through its effects on cellular telomerase activity and telomere length. In the experiments, the cell line of hepatocytes L-02 was divided into three groups supplemented with sodium selenite at final concentrations of 0, 0.5 and 2.5 micromol/L, respectively. Cellular telomerase activity was measured by telomeric repeat amplification protocol and enzymatic luminometric inorganic pyrophosphate detection assay. RT-PCR was used to semi-quantitatively detect human telomerase reverse transcriptase (hTERT) gene expression. The change of telomere length was assayed through flow cytometry and fluorescence in situ hybridization. Results showed that L-02 cells had low telomerase activity and hTERT gene expression level when cultured in the normal way. The cells grew well after 3-week-cultivation in the media supplemented with 0.5 or 2.5 micromol/L sodium selenite. Besides, sodium selenite significantly increased cellular telomerase activity and hTERT gene expression level. The telomere length of L-02 cells was also extended after 4-week-cultivation with sodium selenite. Thus, sodium selenite at nutritional doses could prolong the life span of hepatocytes L-02 through increasing telomerase activity and telomere length. This result provides a possible mechanism for explaining the anti-senescence function of selenium.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1117-22"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effects of site-directed mutagenesis at amino acid residues of GATA-1b different from that of GATA-1a in Xenopus]. [对爪蟾GATA-1b与GATA-1a不同氨基酸残基的定点诱变效应]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Xiang-Ling Feng, Wei-Dong Liu, Hong Li, Lei Wang, Xu-Yu Yang, Wei-Nong Han, Wen Zhou, Kai-Tai Yao
{"title":"[Effects of site-directed mutagenesis at amino acid residues of GATA-1b different from that of GATA-1a in Xenopus].","authors":"Xiang-Ling Feng,&nbsp;Wei-Dong Liu,&nbsp;Hong Li,&nbsp;Lei Wang,&nbsp;Xu-Yu Yang,&nbsp;Wei-Nong Han,&nbsp;Wen Zhou,&nbsp;Kai-Tai Yao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The GATA-1 of Xenopus (xGATA-1), which has two subtypes xGATA-1a and xGATA-1b, is a necessary factor for erythroid differentiation and maturation as similar as that of other GATA-1s. Although both xGATa-1a and xGATA-1b are able to stimulate erythropoiesis, only xGATA-1b is capable of inhibiting neurogenesis in Xenopus embryos. Compared between their structures, xGATA-1a and xGATA-1b are very similar in nucleotide and amino acids composition, but not identical. Therefore, it is responsible for studying the role of the diverse codons between the two genes, so the desired mutations: S(168), H(169) double deletion and point mutation of T(304)-->A, T(359)-->A, were introduced into xGATA-1b gene through site-directed mutagenesis. Then, mRNA from each mutant as well as wtxGATA-1b was co-injected with DN-BR mRNA or separately injected into Xenopus stage 2 embryos, and the role of mutants in erythropoiesis and neurogenesis was analyzed by using animal cap culture system. The results showed that the neural-inhibiting activity of xGATA-1b, but not hematopoiesis-inducing activity, was aborted because of deletion of Ser(168) and His(169) or point mutation of T(359)-->A. So it is demonstrated for the first time that Ser(168) and His(169) or Thr(359)in xGATA-1b may be one of the structural basis for explanting the different function between xGATA-1b and xGATA-1a.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1105-10"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon. 干扰素诱导乙型肝炎病毒转染细胞株基因表达分析。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Wei Xiong, Xun Wang, Xiao-Ying Liu, Li Xiang, Ling-Jie Zheng, Jiang-Xia Liu, Zheng-Hong Yuan
{"title":"Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon.","authors":"Wei Xiong,&nbsp;Xun Wang,&nbsp;Xiao-Ying Liu,&nbsp;Li Xiang,&nbsp;Ling-Jie Zheng,&nbsp;Jiang-Xia Liu,&nbsp;Zheng-Hong Yuan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Infection of hepatitis B virus (HBV) continues to be a significant health problem. alpha interferon (IFN-alpha) and gamma interferon (IFN-gamma) have been proven to be effective in inhibiting HBV replication. To study the global effect of HBV persistent existence on IFN induced cellular gene expression, cDNA microarrays dotted with 14 112 human genes were used to examine the transcriptional changes between an HBV DNA transfected cell line (HepG2.2.15) and its parental cell line (HepG2) after the treatment of IFN-alpha or IFN-gamma for 6 h. The results showed that many genes related to cell cycle, proliferation, apoptosis and new ESTs were regulated by IFN-alpha and/or IFN-gamma. Many genes involved in kinase and signal transduction, transcription regulation, antigen presentation and processing were differentially regulated between these two cell lines post IFN-alpha or IFN-gamma treatment. Interestingly, several IFN-differentially regulated genes, such as MyD88 and Diubiquitin, were found to inhibit HBV gene expression, and MyD88 was proved to inhibit HBV replication. Taken together, our results revealed the global effects of HBV persistent existence on IFN-induced cellular gene expression. The novel antiviral genes identified by microarray could be potentially developed as new anti-HBV drugs or for novel therapies.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1053-60"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24122332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma japonicum gene]. 日本血吸虫新基因sjf4的克隆、表达及功能分析
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Liang Zhang, Guo-Feng Cheng, Zhi-Qiang Fu, Ya-Mei Jin, Xin-Gang Feng, Chun-Xiu Yuan, You-Min Cai, Jiao-Jiao Lin
{"title":"[Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma japonicum gene].","authors":"Liang Zhang,&nbsp;Guo-Feng Cheng,&nbsp;Zhi-Qiang Fu,&nbsp;Ya-Mei Jin,&nbsp;Xin-Gang Feng,&nbsp;Chun-Xiu Yuan,&nbsp;You-Min Cai,&nbsp;Jiao-Jiao Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Based on the phenomenon of the natural anti-schistosomiasis in Microtus fortis, the sera from normal Microtus fortis were employed to immunoscreen the cDNA library of adult Schistosoma japonicum (Chinese strain), two positive clones were obtained, RACE technique was further applied to amplify one of the clones, and a cDNA fragment with an ORF was identified. Sequencing revealed that it was a novel gene of Schistosoma japonicum, and it was named SjMF4 (Schistosoma japonicum Microtus fortis 4). Then the structure and functional motifs of SjMF4 were analysed. The gene was subcloned into pET-28a(+) vector; the recombinant protein showed good antigenicity in Western blotting. The gene was further subcloned into eukaryotic expression vector pcDNA3 to construct the DNA vaccine containing SjMF4. Immune experiments in mice showed significant protection that the recombinant plasmid did induce 28.64%+/-3.82% worm reduction and 21.73%+/-3.98% egg reduction than controls against the Schistosoma japonicum cercaria challenge.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1099-104"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Purification of a big defensin from Ruditapes philippinesis and its antibacterial activity]. [菲律宾鲁贝大防御素的纯化及其抑菌活性]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Yu-Xi Wei, Dao-Sen Guo, Rong-Gui Li, Hao-Wen Chen, Pei-Xun Chen
{"title":"[Purification of a big defensin from Ruditapes philippinesis and its antibacterial activity].","authors":"Yu-Xi Wei,&nbsp;Dao-Sen Guo,&nbsp;Rong-Gui Li,&nbsp;Hao-Wen Chen,&nbsp;Pei-Xun Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A novel big defensin was isolated and characterized from the plasma of Ruditapes philippinesis. It was purified to homogeneity by means of precipitation with (NH(4))(2)SO(4), gel-exclusion chromatography, two kinds of cation-exchange chromatography and named RPD-1. Its relative molecular mass was 24.8 kD by means of SDS-PAGE. By means of ABI437 amino acid sequence analyser, its 11 NH(2)-terminal amino acid sequence is AVPDVAFNAYG. Two databank systems (NCBI and EBI/EMBL) was indexed, no sequence with homology to RPD-1 was found. Furthermore, it exhibited strong inhibition on the growth of Gram-negative and -positive bacteria. Minimal inhibitory concentration (MIC) of RPD-1 against Staphylococcus aureus, Bacillus subtilis, Micrococcus tetragenus, Escherichia coli, Vibrio parahaemolyticus, Vibrio anguillarum was 9.6 mg/L, 76.8 mg/L, 38.4 mg/L, 76.8 mg/L, 19.2 mg/L, 19.2 mg/L respectively.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1145-8"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide]. 人B淋巴细胞刺激物c端肽的免疫增强作用。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Rui-Yang Tian, Wei Han, Ying Yu, Yue Chen, Gu-Song Yu, Sheng-Li Yang, Yi Gong
{"title":"[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].","authors":"Rui-Yang Tian,&nbsp;Wei Han,&nbsp;Ying Yu,&nbsp;Yue Chen,&nbsp;Gu-Song Yu,&nbsp;Sheng-Li Yang,&nbsp;Yi Gong","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cDNA of human B lymphocyte stimulator C-terminal peptide (C-BLyS) was amplified by nested PCR from cDNA library of human fetal brain. The expression plasmid pT7450-C-BLyS was constructed and transformed into E. coli BL21 CodonPlus (DE3) RIL which can recognize many rare codons. The C-BLyS protein was expressed as inclusion body in E. coli BL21 CodonPlus (DE3) RIL and the inclusion body of C-BLyS was denatured and then refolded by dialysis and purified by ion exchange chromatography. The refolded and purified C-BLyS can specifically bind with BCMA-Fc, a fusion protein of BLyS receptor and human IgG1 Fc. Furthermore, C-BLyS is proved to be an effective stimulator in mouse splenocytes proliferation in vitro and effective immunostimulant in vivo.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1128-32"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A method for delineation of domains in proteins based on refolding free energy--application to continuous and discontinuous domains]. [一种基于可折叠自由能的蛋白质结构域描述方法——应用于连续和不连续结构域]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Zhi-Qun Xie, Gen-Jun Xu
{"title":"[A method for delineation of domains in proteins based on refolding free energy--application to continuous and discontinuous domains].","authors":"Zhi-Qun Xie,&nbsp;Gen-Jun Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Domain is a protein architecture in a subunit. It might be defined as a basic unit for structure, function, folding, evolution and design. Different combinations of domains lead to the formation of various tertiary structures with various functions for proteins. The delineation of domains for a protein is important both conceptually and practically, which remains up to date a challenging and unsolved problem. Based on the above definition, a method was previously proposed based on refolding free energy to define continuous domains in proteins. By constructing a residue-residue contact matrix, using correspondence analysis, and then selecting optimal partition function of a protein according to refolding free energy and some empirical scoring functions, a new computer program, PDOM, was developed, which was applicable to both continuous and discontinuous domains. When compared with the manual partition results reported by crystallographers, PDOM has achieved an accuracy of 76% on a test data set including 55 protein structures frequently used. The differences in 13 proteins between PDOM, literature as well as SCOP have been discussed extensively.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1090-8"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Prokaryotic expression and characterization of rice Rac protein osRACB]. 水稻Rac蛋白osRACB的原核表达及特性研究
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Min Luo, Chao-Rong Tang, Nai-Hu Wu
{"title":"[Prokaryotic expression and characterization of rice Rac protein osRACB].","authors":"Min Luo,&nbsp;Chao-Rong Tang,&nbsp;Nai-Hu Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As the sole ubiquitous signal GTP-binding protein family in higher plants, Rac genes act as pivotal molecular switches and participate in many regulations of life activities. In order to study the biochemical characteristics of rice Rac protein osRACB, the complete coding sequence of osRACB was cloned into expression vector pET28a and expressed in E. coli BL21(DE3). After induced by 1 mmol/L IPTG at 37 degrees C for 4 h, the fusion protein His-osRACB was produced in a large amount. The fusion protein was purified by Ni(2+)-NTA column and digested by thrombin. After a series of processes including separation and recovery by electrophoresis, renaturation by glutathione and concentration by ultrafiltration, pure osRACB protein in an active form was obtained. The GTP-binding and hydrolyzing assay showed that osRACB has strong GTP special binding and hydrolysis activity regulated by Mg(2+). By comparing it with another rice Rac protein osRACD, it can be concluded that osRACB has stronger GTP-binding activity and weaker hydrolysis activity than osRACD.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1149-54"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Cloning and characterization of a novel cardiac-specific kinase gene p93 related to sarcomere]. [一种与肌瘤相关的心脏特异性激酶基因p93的克隆和表征]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01
Xian-Min Meng, Yong Zhao, Ying-Jie Wei, Hui-Qing Cao, Xiu-Wen Zhao, Dong-Qing Liu, Na Shi, Jin-Feng Ding
{"title":"[Cloning and characterization of a novel cardiac-specific kinase gene p93 related to sarcomere].","authors":"Xian-Min Meng,&nbsp;Yong Zhao,&nbsp;Ying-Jie Wei,&nbsp;Hui-Qing Cao,&nbsp;Xiu-Wen Zhao,&nbsp;Dong-Qing Liu,&nbsp;Na Shi,&nbsp;Jin-Feng Ding","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Myocyte's contraction is regulated by signal transduction pathway composed with many protein factors, but the definite mechanism is still uncertain. A novel cardiac-specific kinase gene which participates in regulation of signal transduction, p93 named, was cloned from adult heart cDNA library. p93, coding a family member of MAPKKK, localized on 1p31.1 based on bioinformatics analyses. Northern blot and 76-tissue array analyses determined that p93 was merely expressed in heart, but was undetectable in other tissues. Immunohistochemical study showed that p93 predominantly localized in the nucleus of cardiac myocytes. In vitro kinase assay indicated that p93 was a functional kinase capable of autophosphorylation. p93 could directly interact with cardiac troponin I by yeast two-hybrid system assessed utilizing bait plasmid containing p93 C-terminus (733 835 aa) and results were further confirmed by co-immunoprecipitation in vivo. Our data suggest that p93 is a cardiac-specific kinase and may play important role in regulation of sarcomeric contraction protein with signal transduction pathway similar ILK.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":"35 12","pages":"1083-9"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24123341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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