N-LMP1转基因小鼠鼻咽特异性调控区构建

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-12-01

Ling Zhang, Ke Lan, Xiang-Ling Feng, Gui-Lin Qiao, Xin-Ming Shen, Yi-Min Shi, Hong Li, Kai-Tai Yao

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引用次数: 0

摘要

为阐明EBV-LMP1在鼻咽癌发生中的作用，构建了N-LMP1基因受EDL-2和PLUNC-p两种特异性调控因子双重调控的表达载体。通过核原显微注射成功构建了N-LMP1相关转基因小鼠模型。共出生58只小鼠，经PCR和Southern blot检测，其中4只小鼠阳性。免疫组化分析显示，N-LMP1蛋白在转基因小鼠鼻咽、舌和前胃中均有表达。

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[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx].

In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.

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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

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