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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica最新文献

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[Purification and partial characterization of luffin P1, a peptide with translational inhibitory activity and trypsin inhibitory activity, from seeds of Luffa cylindrica]. [丝瓜种子中具有翻译抑制活性和胰蛋白酶抑制活性的肽luffin P1的纯化和部分表征]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Feng Li, Hen-Chuan Xia, Xin-Xiu Yang, Wei-Guo Hu, Zhen Li, Zu-Chuan Zhang
{"title":"[Purification and partial characterization of luffin P1, a peptide with translational inhibitory activity and trypsin inhibitory activity, from seeds of Luffa cylindrica].","authors":"Feng Li,&nbsp;Hen-Chuan Xia,&nbsp;Xin-Xiu Yang,&nbsp;Wei-Guo Hu,&nbsp;Zhen Li,&nbsp;Zu-Chuan Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A peptide, luffin P1, from seeds of Luffa cylindrica, was purified by ammonia sulfate precipitation, CM-52 ion exchange chromatography, Blue-gel affinity chromatography and FPLC Mono S ion exchange chromatography. Its molecular weight was 5226.5 as determined by MALDI-TOF-MS analysis. The sequence of N-terminal 11 amino acids of luffin P1 was identical with the partial N-terminal sequence (from G3 to R13) of 6.5K Arg/Glu rich peptide, which was also isolated from the seeds of Luffa cylindrica. Besides, luffin P1 had a very high homology with a trypsin inhibitor, named C2 peptide, from pumpkin seeds. Interestingly, the purified luffin P1 not only showed a strong inhibitory activity on protein synthesis in rabbit reticulocyte lysate cell-free translation system with IC(50) of 0.6 nmol/L, but also had trypsin inhibitory activity with IC(50) of 22 micromol/L.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Cloning, expression and characterization of the hypoxanthine-guanine phosphoribosyltransferase mutants from T. tengcongensis]. [腾家蚕次黄嘌呤-鸟嘌呤磷酸核糖基转移酶突变体的克隆、表达和特性研究]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
De-Lin You, Hong Qu, Qiang Chen, Yang Xing, Xiao-Cheng Gu, Ming Luo
{"title":"[Cloning, expression and characterization of the hypoxanthine-guanine phosphoribosyltransferase mutants from T. tengcongensis].","authors":"De-Lin You,&nbsp;Hong Qu,&nbsp;Qiang Chen,&nbsp;Yang Xing,&nbsp;Xiao-Cheng Gu,&nbsp;Ming Luo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Based on a predicted three-dimensional structure of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) from Thermoanaerobacter tengcongensis, three mutants of HGPRT were designed to modify the purine specificity of HGPRT. Site-directed mutagenesis was used to generate the three mutants, K(133)A, K(133)S, K(133)T. Wild type HGPRT and its mutants were expressed E. coli in BL21 (DE3) pLysS, and the expression products of them reached about 30% of the total protein. The molecular weight of the recombinant proteins was 22 kD. The specific activities of the enzymes were determined. Catalytic activities of mutants K(133)A, K(133)S, K(133)T retained only 4%, 1.1%, 2.7% activities, respectively, of the wild type for hypoxanthine, 1.7% 0.6% 1% activities, respectively, of wild type for guanine. However, the three mutants showed 24-fold, 7-fold, 18-fold activities, respectively, of the wild type for xanthine, and 650-fold, 210-fold, 380-fold activities, respectively, of the wild type for adenine. Comparison of kinetic data for purified recombinant mutant with wild-type HGPRT showed significant difference in the catalytic efficiency (kcat/Km) for purine, xanthine and adenine, the mutants exhibiting more than 40 to 50-fold higher kcat/Km, as a result of nearly 4 to 5-fold decrease in Km, compared with wild-type. These results demonstrate that a single amino acid substitution in HGPRT at the active site can significantly modify the specificity for binding purine.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[XBP-1 enhances the transcriptional activity of estrogen receptor alpha]. [XBP-1增强雌激素受体α的转录活性]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Li-Hua Ding, Qi-Nong Ye, Jian-Hua Zhu, Jing-Hua Yan, Hong-Jun Zhong, Zong-Hua Wang, Cui-Fen Huang
{"title":"[XBP-1 enhances the transcriptional activity of estrogen receptor alpha].","authors":"Li-Hua Ding,&nbsp;Qi-Nong Ye,&nbsp;Jian-Hua Zhu,&nbsp;Jing-Hua Yan,&nbsp;Hong-Jun Zhong,&nbsp;Zong-Hua Wang,&nbsp;Cui-Fen Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The estrogen receptor (ERalpha) is a member of a large superfamily of nuclear receptors that regulates the transcription of estrogen-responsive genes. Several recent studies have demonstrated that human X-box binding protein 1 (XBP-1) mRNA expression is associated with ERalpha status in breast tumors. More recently, two forms of XBP-1 were identified due to their unique splicing. The two splicing variants of XBP-1 were designated XBP-1S and XBP-1U, respectively. In this study, the coding sequences of XBP-1S and XBP-1U were cloned respectively into the expression vector pcDNA3 harboring FLAG epitope, generating the recombinant plasmids pcDNA3-FLAG-XBP-1S and pcDNA3-FLAG-XBP-1U. Western blot analysis showed that both XBP-1S and XBP-1U were expressed in mammalian cells. To determine the effects of XBP-1S and XBP-1U on the transcriptional activity of ERalpha, MDA-MB-231 breast cancer cells were cotransfected with the expression vectors for ERalpha and either pcDNA3-FLAG-XBP-1S or pcDNA3-FLAG-XBP-1U. The results indicated that XBP-1S and XBP-1U enhanced ERalpha-mediated transcriptional activities in a hormone-independent manner. GST pull-down assay showed that both XBP-1S and XBP-1U interacted with ERalpha. These data suggest that XBP-1S and XBP-1U may play an important role in breast cancer growth and progression through ERalpha signaling.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CC chemokine receptor-coupled signalling pathways. CC趋化因子受体偶联信号通路。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
David C New, Yung H Wong
{"title":"CC chemokine receptor-coupled signalling pathways.","authors":"David C New,&nbsp;Yung H Wong","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The isolation and characterization of multiple CC chemokine receptors (CCRs) in a wide range of tissues and cells signifies the functional diversity of CC chemokines. The realization that multiple chemokines activate individual receptors and that some chemokines are functional at several different CCRs, indicates that interplay between a complex network of intracellular pathways is required for the full expression of the physiological function of each ligand. In different cellular environments, chemokines can regulate distinct second messengers or even positively or negatively regulate the same signal transduction pathway. The specific interactions between many signalling molecules have been discerned in an increasing number of cellular systems and this information is being used to explain the physiological actions of chemokines. This review will attempt to summarize recent research by many groups that has revealed numerous subtleties of the CC chemokine-coupled signalling pathways.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The characterization of Ca(2+)-calmodulin independent phosphorylation of myosin light chains by a fragment from myosin light chain kinase. 肌凝蛋白轻链激酶片段对Ca(2+)-钙调蛋白不依赖于肌凝蛋白轻链磷酸化的表征。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Jing-Xian Yang, Xiao-Ming Wang, Ze-Yao Tang, Hua Chen, Hong Xu, Yuan Lin
{"title":"The characterization of Ca(2+)-calmodulin independent phosphorylation of myosin light chains by a fragment from myosin light chain kinase.","authors":"Jing-Xian Yang,&nbsp;Xiao-Ming Wang,&nbsp;Ze-Yao Tang,&nbsp;Hua Chen,&nbsp;Hong Xu,&nbsp;Yuan Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A constitutively active myosin light chain kinase (MLCK) fragment (MLCKF) was found to phosphorylate myosin light chains (MLC(20)) in a Ca(2+)-CaM independent way more effectively than the intact MLCK. The MLCKF was prepared by tryptic digestion of MLCK. Western blot was used to demonstrate the homogeneity of trypsin-digested MLCKF and intact MLCK. Phosphorylation of MLC(20) was detected by Gly-PAGE and Scoin Image Software, and Mg(2+)-ATPase activity of myosin was measured with spectrophotometry. Our results indicated that Ca(2+)-CaM independent phosphorylation of myosin (CIPM) by MLCKF was more efficient than CIPM by MLCK and less efficient than Ca(2+)-CaM dependent phosphorylation of myosin (CDPM) by MLCK in phosphorylating MLC(20) and stimulating myosin Mg(2+)-ATPase activity; both CIPM by MLCKF and CIPM by MLCK were less influenced by the rise of incubation-temperature, the prolonging of incubation-time, the increase of ionic strength of KCl and less sensitive to MLCK inhibitor ML-9 1-(5-chloronaphthalene-1-sulfonyl) -1H-hexahydro-1,4-diazepine than CDPM by MLCK. The differences were statistically significant ((P)<0.01, or (P)<0.05). The results may be valuable to further investigating the mechanisms of sustained tension characterized by less energy consumption.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Researching a novel NPC-related candidate suppressor gene BRD7 by two-dimensional gel electrophoresis and MALDI-TOF-MS]. [通过二维凝胶电泳和MALDI-TOF-MS研究一种新的npc相关候选抑制基因BRD7]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Cong Peng, Song-Ping Liang, Chen Tan, Hong-Bin Lv, He Huang, Min Zhou, Rong Wang, Xiao-Lin Li, Gui-Yuan Li
{"title":"[Researching a novel NPC-related candidate suppressor gene BRD7 by two-dimensional gel electrophoresis and MALDI-TOF-MS].","authors":"Cong Peng,&nbsp;Song-Ping Liang,&nbsp;Chen Tan,&nbsp;Hong-Bin Lv,&nbsp;He Huang,&nbsp;Min Zhou,&nbsp;Rong Wang,&nbsp;Xiao-Lin Li,&nbsp;Gui-Yuan Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>BRD7 was isolated through cDNA representational difference analysis (RDA) (GenBank accession No. AF152604). Previous studies showed that BRD7 gene was down-regulated in nasopharyngeal carcinoma (NPC) cells and tissues, and three cSNPs (coding-region single nucleotide polymorphisms) were found on BRD7. In addition, six BRD7-interacting proteins were identified by yeast two-hybrid system. To study the function of this gene on carcinogenesis in NPC, BRD7 gene was transfected into HNE1 cells low-expressed BRD7 by using liposomes and a stable cell line over-expressing BRD7 was established. After two-dimensional gel electrophoresis(2-DE), twenty differentially expressed proteins were identified by MALDI-TOF-MS including argininosuccinate lyase, metalloproteinase inhibitor-2 precursor, proteaseome activator28 beta subunit, thyroid transcriptional factor-1, cyclinH (MO15-associated protein), and so on. These differentially expressed proteins are related to cell cycling, transcription regulation, signaling pathway etc. Therefore, BRD7 may exert its functions by mediating differential expression of these proteins.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Baculovirus p74 gene is a species-specific gene]. 杆状病毒p74基因是一种物种特异性基因。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Wu-Wei Wu, Jing-Wen Wang, Fei Xie, Qing-Xin Long, Xun-Zhang Wang
{"title":"[Baculovirus p74 gene is a species-specific gene].","authors":"Wu-Wei Wu,&nbsp;Jing-Wen Wang,&nbsp;Fei Xie,&nbsp;Qing-Xin Long,&nbsp;Xun-Zhang Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The p74 gene of Autographa californica multicasid nucleopolyhedrovirus (AcMNPV) bacmid was knockouted and substituted by the p74 gene of Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV), using RecA-mediated homologous recombination in the E. coli. No selection marker, which might influence the expression and function of p74 gene, was left in the modified p74 locus. The promoter of AcMNPV p74 gene directly controlled the expression of SpltMNPV p74 gene in the recombinant AcMNPV bacmid-polhSL74. RT-PCR showed that the substituted p74 gene was transcribed. Bioassay showed that the recombinant virus AcMNPV bacmid-polhSL74 could not infect the Argyrogramma agnata larvae per os, and thus showing the p74 gene is species-specific.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Identification of interaction and interaction domains between neuroglobin and Na(+), K(+)-ATPase beta2 subunit]. [神经球蛋白与Na(+)， K(+)- atp酶β 2亚基的相互作用和相互作用域的鉴定]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Wen-Lin Xu, Chun-Li Wang, Zhi-Yong Liao, Yong-Liang Zhang, Li-Hong Yu, Fan-Wei Meng, Xin-Xing Wang, Fan-Wei Meng, Zhao-Yun Yin, Ling-Jia Qian, Cheng-Gang Zhang
{"title":"[Identification of interaction and interaction domains between neuroglobin and Na(+), K(+)-ATPase beta2 subunit].","authors":"Wen-Lin Xu,&nbsp;Chun-Li Wang,&nbsp;Zhi-Yong Liao,&nbsp;Yong-Liang Zhang,&nbsp;Li-Hong Yu,&nbsp;Fan-Wei Meng,&nbsp;Xin-Xing Wang,&nbsp;Fan-Wei Meng,&nbsp;Zhao-Yun Yin,&nbsp;Ling-Jia Qian,&nbsp;Cheng-Gang Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pre-transformed human fetal brain cDNA library was used to screen the protein interacting with neuroglobin by using yeast two hybrid system III from ClonTech Inc. The protein encoded by one of the clones interacting with neuroglobin (NGB) was confirmed to be the C terminus of the Na(+), K(+)-ATPase beta2 subunit (NKA1b2) based on amino acid sequences. Then the full-length coding region cDNA sequence of NKA1b2 was obtained from human fetal brain cDNA library by PCR. A set of experiments were designed to test the interaction between NGB and NKA1b2. Interaction between NGB and NKA1b2 was confirmed by binding assay in vitro. Furthermore, the interaction was also proved by co-immunoprecipitation test in vivo. Moreover, the structure integrity of neuroglobin was found to be essential for the interaction between NGB and NKA1b2 by yeast two hybrid method with a series of neuroglobin truncated mutants.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant allergic proteins and their biological functions. 植物过敏蛋白及其生物学功能。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Dong-Dong Li, Shao-Heng He
{"title":"Plant allergic proteins and their biological functions.","authors":"Dong-Dong Li,&nbsp;Shao-Heng He","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pollen, fruit and latex in plants can induce allergic diseases like rhinitis, asthma and hay fever. These are classified into inhalent allergens, ingestent allergens and contactent allergens. Recent research showed that these plant allergens are responsible for specific and vital biological function for plants too. Studies on those allergenic proteins will be beneficial for immunotherapy and botanic research. Currently, research on the plant allergens and their related allergic diseases is setting up a novel and cross-subjects field involving plant molecular biology, immunology and allergy.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of anti-bcr-abl hammerhead ribozyme on bone marrow purging]. [抗bcr-abl锤头核酶对骨髓清除的影响]。
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01
Yong Wu, Yuan-Zhong Chen, Hui-Fang Huang, Ping Chen, Lian-Huang Lu
{"title":"[The effect of anti-bcr-abl hammerhead ribozyme on bone marrow purging].","authors":"Yong Wu,&nbsp;Yuan-Zhong Chen,&nbsp;Hui-Fang Huang,&nbsp;Ping Chen,&nbsp;Lian-Huang Lu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To study effects of a hammerhead ribozyme on chronic myelogenous leukemia (CML) cells and bone marrow purging in vitro, a bcr-abl specific ribozyme gene was introduced into CML and normal bone marrow cells using retroviral transduction. The effects of the ribozyme on primary cells from CML patients were detected by hematopoietic progenitor cell assays, flow cytometry and immunocytochemical methods. Then a model of remission was built up, and the effects of ribozyme on bone marrow purging were detected by leukemia colony assay and nest-PCR after transduction of this model by using the ribozyme. The results showed that ribozyme significantly suppressed the clonogenic growth and the expression of p210 protein in primary cells from CML patients, but did not affect the growth of normal hematopoietic progenitor cells. In the model of remission, ribozyme eliminated the proliferation and the expression of bcr-abl mRNA in residual K562 cells, but did not affect the expression of abl mRNA. The results suggest that anti-bcr-abl ribozyme might be used for bone marrow puring of CML cells.</p>","PeriodicalId":21763,"journal":{"name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22563721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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