Scandinavian Journal of Immunology最新文献_第2页

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70009

Xuelan Wu, Zhiyi Ye

引用次数: 0

On the nature of signal 1 delivered to lymphocytes: A critical response to some considerations put forward in support of the quantum model of T cell activation.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70002

Peter A Bretscher

引用次数: 0

The COVID-19 vaccine ChAdOx1 is opsonized by anti-vector antibodies that activate complement and promote viral vector phagocytosis.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70000

Eija Nissilä, Leo Starck, Elias Aho, Erika Venerandi, Pinja Jalkanen, Katarzyna Leskinen, Pavel Uvarov, Päivi Saavalainen, Ilkka Julkunen, Juha Kotimaa, Karita Haapasalo, Seppo Meri

{"title":"The COVID-19 vaccine ChAdOx1 is opsonized by anti-vector antibodies that activate complement and promote viral vector phagocytosis.","authors":"Eija Nissilä, Leo Starck, Elias Aho, Erika Venerandi, Pinja Jalkanen, Katarzyna Leskinen, Pavel Uvarov, Päivi Saavalainen, Ilkka Julkunen, Juha Kotimaa, Karita Haapasalo, Seppo Meri","doi":"10.1111/sji.70000","DOIUrl":"https://doi.org/10.1111/sji.70000","url":null,"abstract":"The ChAdOx1 nCoV-19 vaccine has been in large-scale use during the COVID-19 pandemic. Limited efficacy compared to mRNA vaccines and certain potential side effects raise the question of whether anti-adenoviral vector antibodies influence immune responses against the vaccine. Complement activation by ChAdOx1 and leukocyte phagocytosis of ChAdOx1 in vitro were studied. Plasma IgG levels against ChAdOx1 and human adenovirus 2 (hAdV2) hexon protein were determined (n = 20) and IgGs from high- and low-titre plasmas were isolated (n = 3). Complement activation was measured as cleavage of C3 by immunoblotting and generation of C3a and sC5b-9 by ELISA. pHrodo-labelled ChAdOx1 was opsonized with complement and IgG, and phagocytosis by isolated blood PMNs in vitro was studied by flow cytometry. The transcriptomic profile of PMN cells exposed to ChAdOx1 was analysed by RNA-seq. ChAdOx1 activated the classical complement pathway in an anti-adenovirus antibody-dependent manner. Generation of the terminal complement complex sC5b-9 in individual sera correlated with anti-hAdV2 hexon and anti-ChAdOx1 IgG levels. Phagocytosis of ChAdOx1 also correlated significantly with anti-hAdV2 hexon IgG, anti-ChAdOx1 IgG and serum sC5b-9 levels. High-titre anti-hAdv2 hexon IgG increased phagocytosis in the presence of normal serum. Anti-vector antibodies induced rapid complement activation and promoted phagocytosis of the ChAdOx1 vaccine by neutrophils. Moreover, transcriptomic analysis revealed upregulation of complement-related genes induced by the ChAdOx1 vaccine in vitro. Anti-adenovirus vector antibodies and complement activation may thus influence the efficacy of the ChAdOx1 vaccine against SARS-CoV-2 and be also involved in vaccine-related side effects.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":"101 2","pages":"e70000"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NK cell-based immunotherapy for hepatocellular carcinoma: Challenges and opportunities.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.13433

Pei Guo, Liyuan Zhong, Tao Wang, Weijia Luo, Aiqiang Zhou, Deliang Cao

引用次数: 0

Combined inhibition of membrane receptors as therapeutic targets in rheumatoid arthritis.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70006

Guilherme Pegas Teixeira, Jonathas Albertino de Souza Oliveira, Leandro Rocha, Robson Xavier Faria

引用次数: 0

Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70004

Abbas Habibalahi, Ayad G Anwer, Aline Knab, Shane T Grey, Ewa M Goldys, Jared M Campbell

{"title":"Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.","authors":"Abbas Habibalahi, Ayad G Anwer, Aline Knab, Shane T Grey, Ewa M Goldys, Jared M Campbell","doi":"10.1111/sji.70004","DOIUrl":"10.1111/sji.70004","url":null,"abstract":"Evaluating immune status is a challenging and time-consuming process that involves analysing various biomarkers through numerous assays. The sensitive label-free technique of multispectral imaging of cell autofluorescence involves directly assessing the molecular composition of cells to gather biological information. Cells were cultured in RPMI 1640 modified media supplemented with penicillin-streptomycin and 10% foetal bovine serum at 37°C, with 5% CO2 and 95% humidity. Activation and differentiation was confirmed using immunofluorophores against relevant markers. Multispectral microscopy utilized defined spectral regions, which spanned the excitation (345-476 nm) and emission (414-675 nm) wavelength ranges. In total, 56 distinct spectral channels were applied. These channels cover the spectrum of several fluorophores notably NAD(P)H and flavins, whose concentrations depend on cellular metabolism. We identified distinct spectral signatures for characterizing cells from the Jurkat, Ramos, THP-1, and HL-60 immune cell lines. These signatures correspond to four major immune cell types: T cells (Lymphocytes), B cells (Lymphocytes), monocytes and neutrophils. Moreover, our investigation explored the potential identification of both activated and resting forms of these cells, including the discrimination of M0, M1 and M2 polarized macrophages. Classification accuracy ranged from 92% to 100% based on receiver operator characteristic area under the curve (ROC AUC) assessment. These results indicate that the multispectral evaluation of cell autofluorescence is applicable for characterization of immune status. This includes the assessment of cell types and their activation status, all achievable through a single non-invasive assay.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":"101 2","pages":"e70004"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central Tolerance or Central Adaptation?

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70011

Masoud H Manjili

引用次数: 0

Immune Checkpoint Receptor Expression Profiles of MAIT Cells in Moderate and Severe COVID-19.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70008

Matyas Meggyes, David U Nagy, Ildiko Toth, Timoteus Feik, Beata Polgar, Iyad Saad Al Deen, David Sipos, Laszlo Szereday, Agnes Peterfalvi

{"title":"Immune Checkpoint Receptor Expression Profiles of MAIT Cells in Moderate and Severe COVID-19.","authors":"Matyas Meggyes, David U Nagy, Ildiko Toth, Timoteus Feik, Beata Polgar, Iyad Saad Al Deen, David Sipos, Laszlo Szereday, Agnes Peterfalvi","doi":"10.1111/sji.70008","DOIUrl":"10.1111/sji.70008","url":null,"abstract":"MAIT cells are one of the largest unconventional T cell populations and, recruited to the site of infection, play both protective and pathogenic roles during pulmonary viral infections. MAIT cell activation patterns change significantly during COVID-19, with a notable decrease in their frequency in peripheral blood of severe cases. In the present study, we aimed to investigate the expression profiles of various immune checkpoint pathways on MAIT, MAIT-like and non-MAIT cells in moderate and severe COVID-19 patients undergoing cytokine storm. Despite numerous studies comparing MAIT cell characteristics based on COVID-19 disease severity, none have delved into the critical differences in MAIT cell immune checkpoint profiles between moderate and severe COVID-19 patients, all experiencing a cytokine storm. Flow cytometry was used to analyse peripheral blood mononuclear cells from a cohort of 35 patients, comprising 18 moderate and 17 severe cases, alongside 14 healthy controls. Our investigation specifically focuses on severe COVID-19 presentations, revealing a marked deletion of MAIT cells. Further exploration into the regulatory dynamics of MAIT cell functionality reveals shifts in the expression profiles of critical immune checkpoint receptors, notably PD-1 and CD226. In severe COVID-19 patients, MAIT cells showed a significant decrease in the expression of CD226, whereas MAIT-like and non-MAIT cells demonstrated a significant increase in the expression of PD-1 compared to healthy individuals. The expression of the TIGIT receptor remained unaltered across all investigated groups. Our findings contribute to the existing knowledge by elucidating the changes in MAIT cell subpopulations and their potential role in COVID-19 disease severity.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":"101 2","pages":"e70008"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral monocyte subsets are altered during gestation in oocyte donation pregnancy complicated with pre-eclampsia.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.13432

Xuezi Tian, Jia Li, Kim van Bentem, Ciska Lindelauf, Johanna M Kapsenberg, Carin van der Keur, Lisa E E L O Lashley, Vincent van Unen, Dave L Roelen, Frits Koning, Michael Eikmans, Marie-Louise P van der Hoorn

{"title":"Peripheral monocyte subsets are altered during gestation in oocyte donation pregnancy complicated with pre-eclampsia.","authors":"Xuezi Tian, Jia Li, Kim van Bentem, Ciska Lindelauf, Johanna M Kapsenberg, Carin van der Keur, Lisa E E L O Lashley, Vincent van Unen, Dave L Roelen, Frits Koning, Michael Eikmans, Marie-Louise P van der Hoorn","doi":"10.1111/sji.13432","DOIUrl":"10.1111/sji.13432","url":null,"abstract":"Oocyte donation (OD) pregnancies show a higher fetal-maternal incompatibility and a higher risk of developing pre-eclampsia (PE) than autologous pregnancies. As maternal monocytes play a role in the tolerization of the allogeneic fetus, the aim of this study was to analyse monocyte phenotypes in healthy and PE OD pregnancies. We collected maternal peripheral blood at different gestational time points in healthy (n = 10) and PE (n = 5) OD pregnancies. Fetal-maternal human leukocyte antigen (HLA) mismatches were calculated. We used a 35-colour antibody panel for Aurora spectral flow cytometry to analyse the composition and surface marker expression of monocyte subsets. Expression of CD38 on intermediate monocytes significantly increased throughout gestation in healthy OD pregnancies. Compared with the healthy group, the PE group exhibited even higher CD38 expression on monocyte subsets, with statistical significance. Immune inhibiting receptors CD85j (LILRB1) and CD85d (LILRB2), as well as monocyte recruitment regulating molecules CCR2 and CD91, also showed significantly enhanced expression on monocyte subsets during PE. When comparing healthy and PE OD only in pregnancies with high HLA mismatches, the different CD38 and CD85j expression in monocyte subsets was still significant. In conclusion, in healthy OD pregnancies, the upregulated CD38 expression might reflect a proinflammatory condition specifically at the third trimester. In PE OD pregnancies, expression of both inflammatory and immune regulatory markers is increased in maternal peripheral monocyte subsets. The elevated expression of CCR2 and CD91 on these subsets might reflect monocyte chemotaxis and the effect from systemic vascular dysfunction at the late stage of PE.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":"101 2","pages":"e13432"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of T-cell repertoire by flow cytometric analysis in primary immunodeficiencies with DNA repair defects.

IF 4.1 4区医学

Scandinavian Journal of Immunology Pub Date : 2025-02-01 DOI: 10.1111/sji.70003

Betul Gemici Karaaslan, Zeynep Hizli Demirkale, Isilay Turan, Sezin Aydemir, Zeynep Meric, Zuleyha Taskin, Ozgur Can Kilinc, Nihan Burtecene, Birol Topcu, Esra Yucel, Cigdem Aydogmus, Haluk Cokugras, Ayca Kiykim

{"title":"Evaluation of T-cell repertoire by flow cytometric analysis in primary immunodeficiencies with DNA repair defects.","authors":"Betul Gemici Karaaslan, Zeynep Hizli Demirkale, Isilay Turan, Sezin Aydemir, Zeynep Meric, Zuleyha Taskin, Ozgur Can Kilinc, Nihan Burtecene, Birol Topcu, Esra Yucel, Cigdem Aydogmus, Haluk Cokugras, Ayca Kiykim","doi":"10.1111/sji.70003","DOIUrl":"10.1111/sji.70003","url":null,"abstract":"The group of patients with DNA-repair-defects increases susceptibility to infections due to impaired repertoire diversity. In this context, we aimed to investigate the TCRvβ-repertoire by flow cytometric analysis and its correlation with clinical entities in a group of IEI patients with DNA repair defects. Peripheral lymphocyte subset and TCRvβ-repertoire analyses were performed by flow cytometric analysis. The aim was to explore the changing TCR-Vβ-repertoire that can predict some clinical entities by investigating the repertoire using flow-cytometric-analysis-based TCR-Vβ and its interaction with clinical entities in a group of IEI patients with DNA repair defects. TCR-repertoire of the patients with DNA-repair-defects and healthy controls was analysed with flow-cytometer. The potential of flow-cytometric analysis of the TCR repertoire as a practical and easily accessible clinical prediction method was investigated. Thirty-nine-IEI patients with DNA-repair-defects and 15 age-matched healthy-controls were included in this study. Peripheral lymphocyte subset and TCR-Vβ repertoire analyses were performed by flow cytometry. Compared to the control group, 9 out of 24 clones (37.5%) exhibited a statistically significant reduction, while only 3 clones showed a statistically significant increase (p < 0.05). Preferential use of vβ-genes was associated with some clinical entities. Lower TCR-vβ-9 and TCR-vβ23, higher TCR-vβ7.2 were found in the patients with pneumonia (n = 13) (p = 0.018, p = 0.044 p = 0.032). AT patients with pneumonia had lower TCR-vβ-9 clone than patients without pneumonia (p = 0.008). Skewed proliferation of most TCR-vβ clones was seen DNA-repair-defects, especially AT. In addition, this study showed that preferential use of TCR-vβ genes could be predictive for some clinical entities.","PeriodicalId":21493,"journal":{"name":"Scandinavian Journal of Immunology","volume":"101 2","pages":"e70003"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0