SAGE Open Medicine最新文献

{"title":"Compliance and associated factors of personal protective equipment among sanitary workers in selected public hospitals, Eastern Ethiopia: A cross-sectional study design.","authors":"Sina Temesgen Tolera, Tesfaye Gobena, Abraham Geremew, Elka Toseva, Nega Assefa","doi":"10.1177/20503121241308303","DOIUrl":"10.1177/20503121241308303","url":null,"abstract":"<p><strong>Background: </strong>Adherence to the proper use of protective personal equipment (PPE) in health care facilities including public hospitals is challenging among sanitation workers(SWs) across the world in general and in developing countries in particular. Despite the emphasis inline up on various policies and guidelines for PPE use implementation, inconsistent use of PPE, disobedience to PPE regulations, negligence, ignorance, discomfort, and lacking infection prevention and control (IPC) practice have been identified as main associated factors. All these and other factors contributing for the non-compliance of PPE practice among SWs within the hospitals in nations with limited resources such as Ethiopia, as well as study regions. Thus, such non-adherence or improper application of PPE is a major concern, and ultimately the consequences of unworthy PPE use has had an influence on the health and safety of sanitary workers.</p><p><strong>Objective: </strong>The aim of this study was to assess compliance and associated factors of personal protective equipment among sanitary workers in selected public hospitals, eastern Ethiopia.</p><p><strong>Methods: </strong>A cross-sectional research design used with mixed of quantitative and qualitative data. Surveys were conducted on 809 hospital sanitary workers from May-to-August, 2023. Face-to-face interview was conducted for the quantitative data. Sixteen Key Informant interviews were participated. Field observation also conducted. Epi Data version 3.1 was used for data import, while Stata version 17 MP was used for analysis. Multilevel binary and multivariable regression were for the crude odds ratio and adjusted odds ratio. Variables were analyzed at four levels: Model-0, Model-1, Model-2, and Model-3 for outcome, individual level, hospital level, and individual and hospital levels, respectively. Of these, only model 3 was reported for the interpretation. The cut-point of <i>p</i>-value for crude odds ratio and adjusted odds ratio at model 3 were 0.20 and 0.05, respectively, with a 95% confidence interval reported.</p><p><strong>Result: </strong>Out of 809 sanitary workers, 729 (90.11%) of them were responded. The prevalence of compliance and noncompliance with protective personal equipment practice among sanitary workers were 46.78(95% CI: 43.11%-50.47%) and 53.22% (95% CI: 50.19%-57.11%), respectively. Multivariable multilevel analysis of model 3 shows that the overall variation for compliance of protective personal equipment practice between sanitary workers from hospitals to hospitals was 26.66%. The model also found that those had daily supervision (AOR = 13.71, 3.18-59.11), good infection prevention and control practice (AOR = 11.34, 1.97-65.24), and perceived less severity of protective personal equipment (AOR = 1.46, 0.85-2.59) were more likely to increase protective personal equipment practice.</p><p><strong>Conclusion: </strong>The study concluded that improper personal protective eq","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241308303"},"PeriodicalIF":2.3,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of SGLT-2 inhibitors on clinical and biological hyperandrogenism and menstruation irregularities in patients with polycystic ovary syndrome: A systematic review of randomized trials.","authors":"Houcem Elomma Mrabet, Houda Ben Salem, Taieb Ach, Asma Ben Abdelkarim, Wafa Alaya","doi":"10.1177/20503121241308997","DOIUrl":"10.1177/20503121241308997","url":null,"abstract":"<p><strong>Introduction: </strong>Polycystic ovary syndrome is a common chronic condition characterized by insulin resistance and hyperandrogenism, leading to significant health risks and impaired quality of life. Sodium-glucose transporter type 2 inhibitors have shown promise in improving the metabolic profile of women with polycystic ovary syndrome. However, their impact on hormonal parameters and cycle disorders remains uncertain.</p><p><strong>Methods: </strong>This systematic review analyzed randomized clinical trials published up to 1 December 2023, comparing sodium-glucose transporter type 2 inhibitors to metformin, other antidiabetic agents, or placebo in women with polycystic ovary syndrome. The primary outcomes were changes in total testosterone, free androgen index, dehydroepiandrosterone sulfate, delta-4 androstenedione, and cycle disorders.</p><p><strong>Results: </strong>Five randomized studies were included, evaluating canagliflozin, dapagliflozin, licogliflozin, or empagliflozin against metformin, exenatide, or placebo, with a total of 214 participants. Improvements in total testosterone and dehydroepiandrosterone sulfatewere observed in some studies, but the effects were inconsistent across drugs and outcomes. Additionally, two studies reported beneficial effects on cycle disorders.</p><p><strong>Conclusions: </strong>Sodium-glucose transporter type 2 inhibitors appear to have a potential but variable impact on hormonal parameters in women with polycystic ovary syndrome. However, larger and longer-duration studies are needed to fully elucidate their long-term efficacy in addressing hyperandrogenism and improving overall outcomes in these patients.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241308997"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-alcoholic fatty liver disease among people living with HIV on long-term antiretroviral therapy in Indonesia: Prevalence and related factors.","authors":"Hikmat Pramukti, Evy Yunihastuti, Rino A Gani, Ikhwan Rinaldi, Irsan Hasan, Suzy Maria","doi":"10.1177/20503121241292678","DOIUrl":"10.1177/20503121241292678","url":null,"abstract":"<p><strong>Background/objectives: </strong>As people with human immunodeficiency virus experience longer life expectancy, other causes of morbidity and mortality are being increasingly identified. The incidence of non-alcoholic fatty liver disease has recently been on the rise in Indonesia. People with human immunodeficiency virus on antiretroviral therapy are also at an increased risk of having non-alcoholic fatty liver disease. The study aimed to define the prevalence and factors associated with non-alcoholic fatty liver disease in people with human immunodeficiency virus on stable antiretroviral therapy.</p><p><strong>Methods: </strong>A cross-sectional study of people with human immunodeficiency virus, on antiretroviral therapy, age younger than 18 years old, and without hepatitis co-infection was conducted at the human immunodeficiency virus Integrated Clinic Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Non-alcoholic fatty liver disease was diagnosed using transient elastography with associated controlled attenuation parameter examination (diagnostic cutoff: 238 db/m). A logistic regression test with Poisson regression was used to evaluate factors associated with non-alcoholic fatty liver disease.</p><p><strong>Results: </strong>One hundred and five people with human immunodeficiency virus were included, with a median age of 39 years and 65.7% were men. The prevalence of non-alcoholic fatty liver disease was 52.4%. Factors related to non-alcoholic fatty liver disease were hypertension (aPR: 1.49, 95% CI: 1.03-2.14, <i>p</i> = 0.033) and triglyceride levels (aPR: 1.001, 95% CI: 1.000-1.002, <i>p</i> = 0.024). No human immunodeficiency virus-specific variables were associated with non-alcoholic fatty liver disease.</p><p><strong>Conclusions: </strong>More than half of Indonesian people with human immunodeficiency virus on antiretroviral therapy in this study were found to have non-alcoholic fatty liver disease. Hypertension and increased triglyceride levels were related to non-alcoholic fatty liver disease. Screening for non-alcoholic fatty liver disease should be implemented as a means of early intervention and to prevent complications.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241292678"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of mindfulness meditation on pro-inflammatory biomarkers in patients with end-stage renal disease: A randomized trial.","authors":"Hossam Alhawatmeh, Ishraq Najadat, Issa Hweidi, Sawsan Abuhammad","doi":"10.1177/20503121241308995","DOIUrl":"10.1177/20503121241308995","url":null,"abstract":"<p><strong>Background and aim: </strong>Mindfulness meditation has been inadequately used in patients with end-stage renal disease although it has been effective in reducing pro-inflammatory biomarkers in patients with chronic illnesses. Thus, this study examined mindfulness meditation effect on pro-inflammatory biomarkers and C-reactive protein in patients with end-stage renal disease.</p><p><strong>Materials and methods: </strong>Repeated measures, randomized, control experimental design was used. A convenience sampling technique was used to select the sample from a hospital located in northern Jordan. The participants were randomly distributed into experimental (<i>n</i> = 31) and control (<i>n</i> = 31) groups. During hemodialysis sessions, the group of experiment participants practiced 30 min of the Attentional behavioral cognitive theory version of mindfulness meditation; 3 times a week for 8 weeks). The inflammatory biomarkers including C-reactive protein, tumor necrosis factor-alpha, and interleukine-6 were measured by collecting peripheral blood through venipuncture. These biomarkers were analyzed using the enzyme-linked immunosorbent assay (ELISA) protocol after 5 weeks of the intervention, and at its end (8 weeks). An Excel sheet was used to collect data for participants.</p><p><strong>Results: </strong>Compared to the control condition, mindfulness meditation led to statistically significant reductions in C-reactive protein and tumor necrosis factor over time but a nonsignificant effect on interleukine-6.</p><p><strong>Conclusion: </strong>Study's results support the evidence-based practice recommendation of adding mindfulness meditation as a complementary treatment to the nurse's care plans for patients with end-stage renal disease.</p><p><strong>Trial registration: </strong>Clinical trial.gov; ID: NCT06064708; Date: 09/26/2023.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241308995"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of factors affecting diagnosis of unilateral isolated fungal sphenoid sinusitis.","authors":"Yan Wang, Xiumei Chen, Dajian Li, Yu Zhang, Yan Sun, Xicheng Song","doi":"10.1177/20503121241308694","DOIUrl":"10.1177/20503121241308694","url":null,"abstract":"<p><strong>Background: </strong>To analyze the characteristics and factors influencing the diagnosis of unilateral isolated fungal sphenoid sinusitis.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical data of patients who underwent surgery for unilateral isolated sphenoid sinusitis between June 2020 and May 2023. Data collected included variables such as gender, age, side of the specimen, symptoms, sinus computed tomography findings, pathological results, and complications. The patients were categorized into two groups: the sphenoid sinusitis group and the fungal sphenoid sinusitis group.</p><p><strong>Results: </strong>Among the 84 cases studied, there were 19 males (22.6%) and 65 females (77.4%). Inflammation was observed in 11 patients (13.1%), while fungi were detected in 73 patients (86.9%). Headache was reported in 74 cases (88.1%). Sinus computed tomography findings revealed calcified plaques/spots in 51 cases (60.7%), hyperosteogeny in 75 cases (89.3%), and bone destruction in 11 cases (13.1%). The thickness of the sinus wall ranged from 0.92 to 7.35 mm. The thickness ratio of the bilateral sinus walls ranged from 0.80 to 6.78. The chi-square test indicated significant differences between the two groups in terms of calcified plaques/spots, hyperosteogeny, the thickness of the lesion-side sinus wall, and the ratio of bilateral sinus walls (<i>p</i> < 0.05). Diagnostic tests using the ROC curve demonstrated that calcified plaques/spots, the thickness of the lesion-side sinus wall, and the ratio of bilateral sinus walls had moderate accuracy in diagnosing fungal sphenoid sinusitis.</p><p><strong>Conclusion: </strong>While headache is not a specific symptom of fungal sphenoid sinusitis, sinus computed tomography findings such as calcified plaque/spots, the thickness of the lesion-side sinus wall, and the thickness ratio of bilateral sinus walls are valuable for diagnosis.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241308694"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the relationship between antineoplastic drugs and suicide-related adverse events based on the food and drug administration adverse event reporting system database.","authors":"Nan Zhao, Huimin Wang, Huilin Xu, Xixian Tang, Dedong Cao","doi":"10.1177/20503121241308686","DOIUrl":"10.1177/20503121241308686","url":null,"abstract":"<p><strong>Background: </strong>The potential association between new antineoplastic drugs and an increased risk of suicide-related adverse drug reactions remains unclear. This study aims to utilize the FAERS public database to analyze suicide-related adverse drug reactions associated with common antitumor drugs and to investigate potential risk signals for such adverse drug reactions.</p><p><strong>Methods: </strong>This study was a retrospective analysis utilizing the FAERS database. The FAERS database was examined for reports of suicide-related adverse events associated with antitumor drugs, spanning from 2004 to 2023. To identify and verify adverse event signals, we employed reporting odds ratios, proportional reporting ratios, and Bayesian methods (Bayesian Confidence Propagation Neural Network). Additionally, logistic regression analysis was performed to assess outcomes in tumor patients.</p><p><strong>Results: </strong>A total of 223,781 suicide-related adverse event reports were screened, of which 3790 involved common antitumor drugs. The top five drugs reported were tretinoin (<i>n</i> = 1220), methotrexate (<i>n</i> = 664), celecoxib (<i>n</i> = 505), rituximab (<i>n</i> = 107), and imatinib (<i>n</i> = 105). Risk signal analysis indicated that, with the exception of tretinoin (ROR = 6.317), the reporting odds ratio values for the other drugs were below 2. Among cancer patients, the most frequently reported adverse events included suicidal ideation (<i>n</i> = 233), completed suicide (<i>n</i> = 131), and suicide attempts (<i>n</i> = 97). Regression analysis revealed that risk factors for patient death included indication (OR = 0.967, <i>p</i> < 0.01), gender (OR = 0.57, <i>p</i> < 0.01), and type of adverse event (OR = 4.644, <i>p</i> < 0.01).</p><p><strong>Conclusion: </strong>The findings suggest that antineoplastic drugs may not statistically increase the risk of suicide-related adverse events. However, specific tumor types and suicide-related adverse events may contribute to increased mortality in cancer patients. Further research is warranted to elucidate the risk of suicide-related adverse events in oncology patients.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241308686"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic susceptibility and phenotypic profile of <i>Staphylococcus</i> species isolated from different clinical samples from health facilities: A cross-sectional study.","authors":"Khanda Abdulateef Anwar, Shyar Mustafa Saadalla, Aran Jabar Muhammad Amin, Shad Mahdi Ahmed, Mina Kawa Qadir","doi":"10.1177/20503121241306968","DOIUrl":"10.1177/20503121241306968","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus</i> species are widely distributed in nature and found in various human body sites.</p><p><strong>Objectives: </strong>To determine the antibiotic susceptibility pattern of <i>Staphylococcus</i> species isolated from different clinical samples.</p><p><strong>Methods: </strong>This cross-sectional study was conducted on 400 clinical specimens from conveniently sampled patients seeking healthcare at two health facilities in sulaimani / Iraq. Bacterial isolation and identification were done using conventional techniques, after which the antibiotic susceptibility profile of <i>Staphylococcus</i> species commonly prescribed antibiotics used in treating infections at the facilities was done using the disc diffusion method. Finally, <i>MecA</i>, methicillin-resistant <i>Staphylococcus aureus</i> and macrolides-lincosamide and streptogramin genes with mupirocin-resistant, beta-lactamase and vancomycin-resistance phenotypes were identified.</p><p><strong>Results: </strong><i>Staphylococcus aureus</i> was the prevalent isolated species (<i>n</i> = 197, 49.3%), followed by <i>Staphylococcus hemolyticus</i> (<i>n</i> = 115, 28.8%), <i>Staphylococcus epidermidis</i> (<i>n</i> = 49, 12.3%), <i>Staphylococcus hominis</i> (<i>n</i> = 9.0, 2.3%), <i>Staphylococcus sciuri</i> (<i>n</i> = 8.0, 2.0%) and <i>Staphylococcus lentus</i> (<i>n</i> = 4.0, 1.0%). All isolated species resisted Penicillin G, Ampicillin, Cefotaxime and Cefoxitin. Most of the isolates, 89.5% (<i>n</i> = 358) had the beta-lactamase phenotype, 18.0% (<i>n</i> = 72) had the MecA gene, 2.8% (<i>n</i> = 11) the Mupirocin-resistant phenotype, and 2.0% (<i>n</i> = 8.0) the vancomycin-resistance phenotype. Additionally, 12 isolates had both methicillin-resistant <i>Staphylococcus aureus</i> (66.7%) and macrolides-lincosamide and streptogramin (65.2%) genes. The majority of the patients, 43% (<i>n</i> = 172) were >50 years old and 52.25% (<i>n</i> = 209) males. Also, most samples were from patients with urinary tract infection (<i>n</i> = 73), wound (<i>n</i> = 71), blood (<i>n</i> = 35), sputum (<i>n</i> = 29), pus (<i>n</i> = 28), seminal fluid (<i>n</i> = 27), cerebrospinal fluid (<i>n</i> = 1.0) and stool (<i>n</i> = 1.0). Most isolates that had the MSLb gene were highly significantly resistant to both Clindamycin (94.6%) and Erythromycin (84.7%) (<i>p</i> < 0.001).</p><p><strong>Conclusions: </strong><i>Staphylococcus aureus</i> was the predominant <i>Staphylococcus</i> species isolated from the clinical samples, most of which were resistant to most commonly prescribed antibiotics and had developed resistant genes and phenotypes.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241306968"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weight bearing versus non-weight bearing total contact cast in the management of active Charcot foot: A systematic review.","authors":"Rachna Prem, Vikramman Vignaraja, Thomas Lewis, Basil Budair","doi":"10.1177/20503121241306957","DOIUrl":"10.1177/20503121241306957","url":null,"abstract":"<p><strong>Aim: </strong>Diabetic Charcot neuro-osteoarthropathy carries a significant worldwide disease burden including diabetic foot infection, ulceration and amputation. The current accepted standard of treatment during the active phase of Charcot neuro-osteoarthropathy is offloading with total contact casting; however, controversy remains regarding weight-bearing status during this period.</p><p><strong>Methods: </strong>A systematic review was performed following PRISMA guidelines of Pubmed, EMBASE, MEDLINE and the Cochrane central register of controlled trials for clinical studies from inception until June 2024 investigating weight-bearing and non-weight-bearing total contact casting for active Charcot neuro-osteoarthropathy.</p><p><strong>Results: </strong>Four hundred ninety-three studies were identified in the search strategy of which 5 studies met the inclusion criteria comprising 158 patients. These studies found that allowing patients to weight-bear during total contact casting does not have a negative impact on the healing process. There were no comparative studies between weight-bearing and non-weight-bearing total contact casting.</p><p><strong>Conclusions: </strong>There is limited evidence to support current practice of non-weight bearing in a total contact casting for active Charcot neuro-osteoarthropathy. Allowing patients to weight bear carries advantages to patient independence and quality of life. Further investigation with randomised control trial should be considered to investigate if weight bearing is associated with negative outcomes.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241306957"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose mesenchymal stem cell conditioned medium and extract: A promising therapeutic option for regenerative breast cancer therapy.","authors":"Faezeh Kazemi, Fatemeh Sadeghian, Ali Pirsadeghi, Fatemeh Asadi, Hossein Javdani, Aliakbar Yousefi-Ahmadipour","doi":"10.1177/20503121241306606","DOIUrl":"10.1177/20503121241306606","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer is the second most common cancer and a leading cause of cancer death in U.S. women. The tumor microenvironment, especially nearby adipocytes, plays a crucial role in its progression. Therefore, this study aimed to investigate the effects of human adipose mesenchymal stem cells-derived conditioned medium (SUP) and extract (CE) from on breast cancer cells.</p><p><strong>Methods: </strong>Human adipose-derived mesenchymal stem cells were isolated and characterized by flow cytometry using Cluster of Differentiation (CD) markers (CD34, CD45, CD90, and CD105). The differentiation potential was confirmed via adipogenic and osteogenic induction. MCF-7 and MDA-MB-231 cells were treated with SUP and CE, and cell viability was assessed using the 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay at 24, 48, and 72 h. Doubling time, colony formation, wound healing, and gene expression for key cancer-related genes (<i>TIMP1</i>, <i>TIMP2</i>, <i>MMP2</i>, <i>PDL1</i>, <i>IDO</i>, <i>Bax</i>, caspase 3, and caspase 9) were also evaluated.</p><p><strong>Results: </strong>Both SUP and CE significantly inhibited the viability of MCF-7 and MDA-MB-231 cells, reduced their doubling time, and suppressed colony formation. In wound healing assays, cell migration was notably impaired in MDA-MB-231 cells but less so in MCF-7 cells. Real-time polymerase chain reaction revealed downregulation of TIMP1, MMP2, PDL1, and IDO in MDA-MB-231 cells after treatment, while CE increased certain gene expressions in MCF-7 cells. Bax, caspase 3, and caspase 9 expressions were significantly upregulated in MDA-MB-231 cells but not in MCF-7 cells after treatment.</p><p><strong>Conclusion: </strong>Human adipose-derived mesenchymal stem cells-derived SUP and CE exhibit antitumor effects on breast cancer cells, suggesting a potential therapeutic strategy to suppress tumor progression. Mesenchymal stem cells-SUP and CE could be a safe and novel regenerative approach for breast reconstruction postmastectomy without tumor recurrence risk.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241306606"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating surgical blood loss: A review of current strategies in various clinical settings.","authors":"Alexander D Stoker, Will J Binder, Peter E Frasco, Steven T Morozowich, Layne M Bettini, Andrew W Murray, Megan K Fah, Andrew W Gorlin","doi":"10.1177/20503121241308302","DOIUrl":"10.1177/20503121241308302","url":null,"abstract":"<p><p>The estimation of surgical blood loss is routinely performed during and after surgical procedures and has morbidity and mortality implications related to the risk of under- and over-resuscitation. The strategies for estimating surgical blood loss include visual estimation, the gravimetric method, the colorimetric method, formula-based methods, and other techniques (e.g., cell salvage). Currently, visual estimation continues to be the most widely used technique. In addition, unique considerations exist when these techniques are applied to various clinical settings such as massive transfusion, cardiac surgery, and obstetrics. Ultimately, when using estimated surgical blood loss to guide perioperative fluid management and transfusion thresholds, it is also important to mitigate the risks associated with resuscitation by targeting a goal-directed fluid therapy approach by utilizing markers of fluid-responsiveness to optimize stroke volume (cardiac output) and delivery of oxygen.</p>","PeriodicalId":21398,"journal":{"name":"SAGE Open Medicine","volume":"12 ","pages":"20503121241308302"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}