Reproductive toxicology最新文献_第8页

Biological variability hampers the use of skeletal staining methods in zebrafish embryo developmental toxicity assays 在斑马鱼胚胎发育毒性试验中，生物变异性阻碍了骨骼染色法的使用。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-28 DOI: 10.1016/j.reprotox.2024.108615

Jente Hoyberghs , Jonathan Ball , Maciej Trznadel , Manon Beekhuijzen , Matthew Burbank , Pia Wilhelmi , Arantza Muriana , Nicola Powles-Glover , Ainhoa Letamendia , Steven Van Cruchten

{"title":"Biological variability hampers the use of skeletal staining methods in zebrafish embryo developmental toxicity assays","authors":"Jente Hoyberghs , Jonathan Ball , Maciej Trznadel , Manon Beekhuijzen , Matthew Burbank , Pia Wilhelmi , Arantza Muriana , Nicola Powles-Glover , Ainhoa Letamendia , Steven Van Cruchten","doi":"10.1016/j.reprotox.2024.108615","DOIUrl":"10.1016/j.reprotox.2024.108615","url":null,"abstract":"<div><p>Zebrafish embryo assays are used by pharmaceutical and chemical companies as new approach methodologies (NAMs) in developmental toxicity screening. Despite an overall high concordance of zebrafish embryo assays with <em>in vivo</em> mammalian studies, false negative and false positive results have been reported. False negative results in risk assessment models are of particular concern for human safety, as developmental anomalies may be missed. Interestingly, for several chemicals and drugs that were reported to be false negative in zebrafish, skeletal findings were noted in the <em>in vivo</em> studies. As the number of skeletal endpoints assessed in zebrafish is very limited compared to the <em>in vivo</em> mammalian studies, the aim of this study was to investigate whether the sensitivity could be increased by including a skeletal staining method. Three staining methods were tested on zebrafish embryos that were exposed to four teratogens that caused skeletal anomalies in rats and/or rabbits and were false negative in zebrafish embryo assays. These methods included a fixed alizarin red-alcian blue staining, a calcein staining, and a live alizarin red staining. The results showed a high variability in staining intensity of larvae exposed to mammalian skeletal teratogens, as well as variability between control larvae originating from the same clutch of zebrafish. Hence, biological variability in (onset of) bone development in zebrafish hampers the detection of (subtle) treatment-related bone effects that are not picked-up by gross morphology. In conclusion, the used skeletal staining methods did not increase the sensitivity of zebrafish embryo developmental toxicity assays.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0890623824000820/pdfft?md5=930e1f9638cf4a985c0618aa744f327b&pid=1-s2.0-S0890623824000820-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal intake of paracetamol during pregnancy and biomarkers of male fecundity in young adult sons 母亲在怀孕期间摄入扑热息痛与年幼儿子雄性生殖力的生物标记。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-28 DOI: 10.1016/j.reprotox.2024.108626

Tina Quist Laursen , Cecilia Høst Ramlau-Hansen , Sandra Søgaard Tøttenborg , Zeyan Liew , Gunnar Toft , Anne Gaml-Sørensen , Karin Sørig Hougaard , Jens Peter Ellekilde Bonde , Andreas Ernst

{"title":"Maternal intake of paracetamol during pregnancy and biomarkers of male fecundity in young adult sons","authors":"Tina Quist Laursen , Cecilia Høst Ramlau-Hansen , Sandra Søgaard Tøttenborg , Zeyan Liew , Gunnar Toft , Anne Gaml-Sørensen , Karin Sørig Hougaard , Jens Peter Ellekilde Bonde , Andreas Ernst","doi":"10.1016/j.reprotox.2024.108626","DOIUrl":"10.1016/j.reprotox.2024.108626","url":null,"abstract":"<div><p>Paracetamol is suggested to have endocrine disrupting properties possibly affecting fetal programming of reproductive health that might lead to impaired semen quality and changes in reproductive hormones. In this longitudinal study, we included 1058 young adult men born 1998–2000 into the Danish National Birth Cohort with follow-up at 18–21 years of age. The exposure, maternal intake of paracetamol, was modelled in three ways: dichotomized, trimester-specific, and as duration of exposure categorized into: short (1–2 weeks), medium (3–9 weeks) or long duration (>9 weeks) vs. no intake. Outcomes included semen characteristics, self-measured testis volume, and reproductive hormone levels. We used negative binominal regression to estimate the percentage difference and 95% confidence interval (CI) for each outcome. In total, 547 (48%) sons were prenatally exposed to paracetamol due to maternal intake at least once. Maternal intake of paracetamol during pregnancy was not associated with any of the biomarkers in the dichotomized or trimester-specific exposure models. For duration of exposure, sons of mothers with long duration of maternal intake of paracetamol showed tendencies towards lower semen concentration (-14% [95% CI: -31%; 8%]), a higher proportion of nonprogressive and immotile spermatozoa (8% [95% CI: -4%; 21%]) and higher DNA Fragmentation Index (16% [95% CI: -1%; 36%]) compared to son of mothers with no intake. Maternal intake of paracetamol during pregnancy was not clearly associated with biomarkers of male fecundity in adult sons. However, it cannot be ruled out that long duration of maternal intake of paracetamol might be associated with impaired semen characteristics.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0890623824000935/pdfft?md5=fd1155a9f0d663f7413c7e304c972611&pid=1-s2.0-S0890623824000935-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of ferritin and zinc to adverse infant outcomes among pregnancies with prenatal alcohol exposure in South Africa 南非产前接触酒精的孕妇铁蛋白和锌对婴儿不良结局的影响

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-23 DOI: 10.1016/j.reprotox.2024.108606

Julie M. Hasken , Marlene M. de Vries , Anna-Susan Marais , Philip A. May

{"title":"Contribution of ferritin and zinc to adverse infant outcomes among pregnancies with prenatal alcohol exposure in South Africa","authors":"Julie M. Hasken , Marlene M. de Vries , Anna-Susan Marais , Philip A. May","doi":"10.1016/j.reprotox.2024.108606","DOIUrl":"10.1016/j.reprotox.2024.108606","url":null,"abstract":"<div><p>Nutritional status during pregnancy can impact fetal development, yet less is known about how alcohol may interact with nutritional status to influence infant outcomes. Pregnant women (<em>n</em>=196) completed 2, 24-hour dietary recalls and provided a venous blood sample to be analyzed for liver enzymes (GGT –gamma-glutamyl transferase; ALT –alanine transaminase; and AST –aspartate transferase), iron, ferritin, and zinc concentrations. Infants were assessed at 6 weeks of age. Women who consumed alcohol had significantly higher ferritin levels compared to non-drinkers (51.8 vs. 34.2). While 44% of women had ferritin <30 ug/L (an indicator of iron deficiency), and 24% of women were low in serum iron, and 72% were low in serum zinc. All six drinking measures for 1st trimester and previous week were significantly correlated with GGT and AST levels while 4 out of 6 alcohol measures were associated with levels of ALT and ferritin. At six weeks of age, nearly all physical measures differentiated infants with alcohol exposure from infants without exposure. Controlling for six covariates, maternal ferritin was significantly and inversely associated with infant head circumference (OFC) centile among infants with alcohol exposure. GGT was inversely associated with infant height and weight centile among unexposed infants. Seventy-four percent (74%) of mothers who consumed alcohol were found to be low in serum zinc, yet higher maternal zinc was associated with more dysmorphology. This may indicate that higher zinc status is not protecting the fetus from the teratogenic effects of alcohol. Prenatal alcohol exposure, ferritin, and zinc status influence infant growth and neurodevelopment.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141136970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal exposure to bisphenols, phthalates, perfluoroalkyl acids, and trace elements and their associations with gestational diabetes mellitus in the APrON cohort APrON队列中母亲接触双酚、邻苯二甲酸盐、全氟烷基酸和微量元素的情况及其与妊娠糖尿病的关系。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-21 DOI: 10.1016/j.reprotox.2024.108612

Munawar Hussain Soomro , Gillian England-Mason , Anthony J.F. Reardon , Jiaying Liu , Amy M. MacDonald , David W. Kinniburgh , Jonathan W. Martin , Deborah Dewey , APrON Study Team

{"title":"Maternal exposure to bisphenols, phthalates, perfluoroalkyl acids, and trace elements and their associations with gestational diabetes mellitus in the APrON cohort","authors":"Munawar Hussain Soomro , Gillian England-Mason , Anthony J.F. Reardon , Jiaying Liu , Amy M. MacDonald , David W. Kinniburgh , Jonathan W. Martin , Deborah Dewey , APrON Study Team","doi":"10.1016/j.reprotox.2024.108612","DOIUrl":"10.1016/j.reprotox.2024.108612","url":null,"abstract":"<div><p>The increasing global prevalence of gestational diabetes mellitus (GDM) has been hypothesized to be associated with maternal exposure to environmental chemicals. Here, among 420 women participating in the Alberta Pregnancy Outcomes and Nutrition (APrON) cohort study, we examined associations between GDM and second trimester blood or urine concentrations of endocrine disrupting chemicals (EDCs): bisphenol-A (BPA), bisphenol-S (BPS), twelve phthalate metabolites, eight perfluoroalkyl acids (PFAAs), and eleven trace elements. Fifteen (3.57%) of the women were diagnosed with GDM, and associations between the environmental chemical exposures and GDM diagnosis were examined using multiple logistic and LASSO regression analyses in single- and multi-chemical exposure models, respectively. In single chemical exposure models, BPA and mercury were associated with increased odds of GDM, while a significant inverse association was observed for zinc. Double-LASSO regression analysis selected mercury (AOR: 1.51, CI: 1.12–2.02), zinc (AOR: 0.017, CI: 0.0005–0.56), and perfluoroundecanoic acid (PFUnA), a PFAAs, (AOR: 0.43, CI: 0.19–0.94) as the best predictors of GDM. The combined data for this Canadian cohort suggest that second trimester blood mercury was a robust predictor of GDM diagnosis, whereas blood zinc and PFUnA were protective factors. Research into mechanisms that underlie the associations between mercury, zinc, PFUnA, and the development of GDM is needed.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0890623824000790/pdfft?md5=4b49c98b5b261cd4396dd4fda02d92da&pid=1-s2.0-S0890623824000790-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morinda citrifolia protective effects on paclitaxel-induced testis parenchyma toxicity: An experimental study 海巴戟对紫杉醇诱导的睾丸实质毒性的保护作用：一项实验研究。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-21 DOI: 10.1016/j.reprotox.2024.108611

Sidika Genc , Betul Cicek , Yesim Yeni , Mehmet Kuzucu , Ahmet Hacimuftuoglu , Ismail Bolat , Serkan Yildirim , Himasadat Zaker , Athanasios Zachariou , Nikolaos Sofikitis , Charalampos Mamoulakis , Aristidis Tsatsakis , Ali Taghizadehghalehjoughi

{"title":"Morinda citrifolia protective effects on paclitaxel-induced testis parenchyma toxicity: An experimental study","authors":"Sidika Genc , Betul Cicek , Yesim Yeni , Mehmet Kuzucu , Ahmet Hacimuftuoglu , Ismail Bolat , Serkan Yildirim , Himasadat Zaker , Athanasios Zachariou , Nikolaos Sofikitis , Charalampos Mamoulakis , Aristidis Tsatsakis , Ali Taghizadehghalehjoughi","doi":"10.1016/j.reprotox.2024.108611","DOIUrl":"10.1016/j.reprotox.2024.108611","url":null,"abstract":"<div><p>The current study aimed to investigate the sensitivity of male testis parenchyma cells to chemotherapy agents and the protective effects and mechanisms of Morinda citrifolia (Noni) administration against structural and functional changes before and after chemotherapy (Paclitaxel (PTX)). For this purpose, rats were randomly assigned into four groups (Control = G1, PTX 5 mg/kg = G2; PTX + Noni 10 mg/kg = G3, PTX + Noni 20 mg/kg = G4). PTX was injected intraperitoneally for 4 consecutive weeks, at a dose of 5 mg/kg to all groups except the control group. Then noni was administrated in 10 (G3) and 20 (G4) mg/kg groups orally (gavage) for 14 days. Biochemical analyses, Real-Time Polymerase Chain Reaction (PCR), and immunohistochemical analyses were performed. According to our results, Total Oxidative Stress (TOS) and Malondialdehyde (MDA) were significantly increased in the PTX group (P < 0.01). Superoxide Dismutase (SOD) enzyme activity and Total Antioxidant Capacity (TAC) levels were decreased (P < 0.01). The changes in the rats treated with PTX + Noni 20 mg/kg were noteworthy. The increased levels of IL1-β (Interleukin 1 beta) and TNFα (tumor necrosis factor-alpha) with PTX were down-regulated after treatment with PTX + Noni 20 mg/kg (P < 0.01) (9 % and 5 % respectively). In addition, Noni restored the testicular histopathological structure by reducing caspase-3 expression and significantly (61 %) suppressed oxidative DNA damage and apoptosis (by regulating the Bax (bcl-2-like protein 4)/Bcl-2 (B-cell lymphoma gene-2) ratio). In conclusion, Noni reduced cellular apoptosis and drastically changed Caspase 8 and Bax/Bcl-2 levels. Furthermore, it considerably decreases oxidative damage and can be used in testicular degeneration.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-cellular engineered living systems to assess reproductive toxicology 评估生殖毒理学的多细胞工程活体系统。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-16 DOI: 10.1016/j.reprotox.2024.108609

Isabella Lopez, George A. Truskey

{"title":"Multi-cellular engineered living systems to assess reproductive toxicology","authors":"Isabella Lopez, George A. Truskey","doi":"10.1016/j.reprotox.2024.108609","DOIUrl":"10.1016/j.reprotox.2024.108609","url":null,"abstract":"<div><p>Toxicants and some drugs can negatively impact reproductive health. Many toxicants haven’t been tested due to lack of available models. The impact of many drugs taken during pregnancy to address maternal health may adversely affect fetal development with life-long effects and clinical trials do not examine toxicity effects on the maternal-fetal interface, requiring indirect assessment of safety and efficacy. Due to current gaps in reproductive toxicological knowledge and limitations of animal models, multi-cellular engineered living systems may provide solutions for modeling reproductive physiology and pathology for chemical and xenobiotic toxicity studies. Multi-cellular engineered living systems, such as microphysiological systems (MPS) and organoids, model of functional units of tissues. In this review, we highlight the key functions and structures of human reproductive organs and well-known representative toxicants afflicting these systems. We then discuss current approaches and specific studies where scientists have used MPS or organoids to recreate in vivo markers and cellular responses of the female and male reproductive system, as well as pregnancy-associated placenta formation and embryo development. We provide specific examples of organoids and organ-on-chip that have been used for toxicological purposes with varied success. Finally, we address current issues related to usage of MPS, emerging techniques for improving upon these complications, and improvements needed to make MPS more capable in assessing reproductive toxicology. Overall, multi-cellular engineered living systems have considerable promise to serve as a suitable, alternative reproductive biological model compared to animal studies and 2D culture.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypobaric hypoxia causes low fecundity in zebrafish parents and impairment of skeletal development in zebrafish embryos and rat offspring 低压缺氧导致斑马鱼亲本繁殖力低下以及斑马鱼胚胎和大鼠后代骨骼发育受损

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-15 DOI: 10.1016/j.reprotox.2024.108603

Chaobao Chen , Xin Wang , Yajuan Li , Tianwei Zhao , Huan Wang , Yunqi Gao , Yuanzhou Feng , Jing Wang , Lixin Shang , Yongan Wang , Baoquan Zhao , Wu Dong

{"title":"Hypobaric hypoxia causes low fecundity in zebrafish parents and impairment of skeletal development in zebrafish embryos and rat offspring","authors":"Chaobao Chen , Xin Wang , Yajuan Li , Tianwei Zhao , Huan Wang , Yunqi Gao , Yuanzhou Feng , Jing Wang , Lixin Shang , Yongan Wang , Baoquan Zhao , Wu Dong","doi":"10.1016/j.reprotox.2024.108603","DOIUrl":"10.1016/j.reprotox.2024.108603","url":null,"abstract":"<div><p>Hypobaric Hypoxia (HH) negatively affects the cardiovascular and respiratory systems as well as gonadal development and the therefore next generation. This study investigated the effects of HH on zebrafish and SD rats, by exposing them to a low-pressure environment at 6000 m elevation for 30 days to simulate high-altitude conditions. It was indicated that parental zebrafish reared <em>amh</em> under HH had increased embryo mortality, reduced hatchability, and abnormal cartilage development in the offspring. Furthermore, the HH-exposed SD rats had fewer reproductive cells and smaller litters. Moreover, the transcriptome analysis revealed the down-regulation of steroid hormone biosynthesis pathways. The expression of the gonad-associated genes (amh, pde8a, man2a2 and lhcgr), as well as the gonad and cartilage-related gene bmpr1a, were also down-regulated. In addition, Western blot analysis validated reduced bmpr1a protein expression in the ovaries of HH-treated rats. In summary, these data indicate the negative impact of HH on reproductive organs and offspring development, emphasizing the need for further research and precautions to protect future generations' health.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure to fine particulate matter 2.5 from wood combustion smoke causes vascular changes in placenta and reduce fetal size 暴露于燃木烟雾中的微粒物质 2.5 会导致胎盘血管变化并减小胎儿大小。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-13 DOI: 10.1016/j.reprotox.2024.108610

Francisca Villarroel , Nikol Ponce , Fernando A. Gómez , Cristián Muñoz , Eder Ramírez , Francisco Nualart , Paulo Salinas

{"title":"Exposure to fine particulate matter 2.5 from wood combustion smoke causes vascular changes in placenta and reduce fetal size","authors":"Francisca Villarroel , Nikol Ponce , Fernando A. Gómez , Cristián Muñoz , Eder Ramírez , Francisco Nualart , Paulo Salinas","doi":"10.1016/j.reprotox.2024.108610","DOIUrl":"10.1016/j.reprotox.2024.108610","url":null,"abstract":"<div><p>During gestation, maternal blood flow to the umbilical cord and placenta increases, facilitating efficient nutrient absorption, waste elimination, and effective gas exchange for the developing fetus. However, the effects of exposure to wood smoke during this period on these processes are unknown. We hypothesize that exposure to PM2.5, primarily sourced from wood combustion for home heating, affects placental vascular morphophysiology and fetal size. We used exposure chambers that received either filtered or unfiltered air. Female rats were exposed to PM2.5 during pre-gestational and/or gestational stages. Twenty-one days post-fertilization, placentas were collected via cesarean section. In these placentas, oxygen diffusion capacity was measured, and the expression of angiogenic factors was analyzed using qPCR and immunohistochemistry. In groups exposed to PM2.5 during pre-gestational and/or gestational stages, a decrease in fetal weight, crown-rump length, theoretical and specific diffusion capacity, and an increase in HIF-1α expression were observed. In groups exposed exclusively to PM2.5 during the pre-gestational stage, there was an increase in the expression of placental genes Flt-1, Kdr, and PIGF. Additionally, in the placental labyrinth region, the expression of angiogenic factors was elevated. Changes in angiogenesis and angiogenic factors reflect adaptations to hypoxia, impacting fetal growth and oxygen supply. In conclusion, this study demonstrates that exposure to PM2.5, emitted from wood smoke, in both pre-gestational and gestational stages, affects fetal development and placental health. This underscores the importance of addressing air pollution in areas with high levels of wood smoke, which poses a significant health risk to pregnant women and their fetuses.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bergenin attenuates triptolide-caused premature ovarian failure in mice based on the antioxidant activity 基于抗氧化活性，小檗碱可减轻三苯氧胺导致的小鼠卵巢早衰。

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-10 DOI: 10.1016/j.reprotox.2024.108608

Yanrong Zhu , Lichen Yao , Yilei Guo , Jing Zhang , Yufeng Xia , Zhifeng Wei , Yue Dai

{"title":"Bergenin attenuates triptolide-caused premature ovarian failure in mice based on the antioxidant activity","authors":"Yanrong Zhu , Lichen Yao , Yilei Guo , Jing Zhang , Yufeng Xia , Zhifeng Wei , Yue Dai","doi":"10.1016/j.reprotox.2024.108608","DOIUrl":"10.1016/j.reprotox.2024.108608","url":null,"abstract":"<div><p><em>Tripterygium wilfordii</em> (TW) preparations have been utilized in China for treating rheumatoid arthritis and autoimmune diseases. However, their clinical use is limited due to reproductive toxicity, notably premature ovarian failure (POF). Our study aimed to investigate the effect and mechanism of bergenin in attenuating POF induced by triptolide in mice. POF was induced in female ICR mice <em>via</em> oral triptolide administration (50 μg/kg) for 60 days. Mice received bergenin (25, 50, 100 mg/kg, i.g.) or estradiol valerate (EV) (0.1 mg/kg, i.g.) daily, 1 h before triptolide treatment. <em>In vitro</em>, ovarian granulosa cells (OGCs) were exposed to triptolide (100 nM) and bergenin (1, 3, 10 μM). Antioxidant enzyme activity, protein expression, apoptosis rate, and reactive oxygen species (ROS) levels were assessed. The results showed that triptolide-treated mice exhibited evident atrophy, along with an increase in atretic follicles. Bergenin (50, 100 mg/kg) and EV (0.1 mg/kg), orally administered, exerted significant anti-POF effect. Bergenin and EV also decreased apoptosis in mouse ovaries. <em>In vitro</em>, bergenin (1, 3, 10 μM) attenuated triptolide-induced OGCs apoptosis by reducing levels of apoptosis-related proteins. Additionally, bergenin reduced oxidative stress through downregulation of antioxidant enzymes activity and overall ROS levels. Moreover, the combined use with Sh-Nrf2 resulted in a reduced protection of bergenin against triptolide-induced apoptosis of OGCs. Together, bergenin counteracts triptolide-caused POF in mice by inhibiting Nrf2-mediated oxidative stress and preventing OGC apoptosis. Combining bergenin with TW preparations may effectively reduce the risk of POF.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal 129S1/SvImJ background attenuates the placental phenotypes induced by chronic paternal alcohol exposure 母体 129S1/SvImJ 背景可减轻父体慢性酒精暴露诱导的胎盘表型

IF 3.3 4区医学

Reproductive toxicology Pub Date : 2024-05-10 DOI: 10.1016/j.reprotox.2024.108605

Sanat S. Bhadsavle, Katherine Z. Scaturro, Michael C. Golding

{"title":"Maternal 129S1/SvImJ background attenuates the placental phenotypes induced by chronic paternal alcohol exposure","authors":"Sanat S. Bhadsavle, Katherine Z. Scaturro, Michael C. Golding","doi":"10.1016/j.reprotox.2024.108605","DOIUrl":"10.1016/j.reprotox.2024.108605","url":null,"abstract":"<div><p>Paternal alcohol use is emerging as a plausible driver of alcohol-related growth and patterning defects. Studies from our lab using an inbred C57Bl/6 J mouse model suggest that these paternally-inherited phenotypes result from paternally programmed deficits in the formation and function of the placenta. The 129S1/SvImJ genetic background is typically more susceptible to fetoplacental growth defects due to strain-specific differences in placental morphology. We hypothesized that these placental differences would sensitize 129S1/SvImJ-C57Bl/6 J hybrid offspring to paternally-inherited fetoplacental growth phenotypes induced by paternal alcohol exposure. Using a limited access model, we exposed C57Bl/6 J males to alcohol and bred them to naïve 129S1/SvImJ dams. We then assayed F1 hybrid offspring for alterations in fetoplacental growth and used micro-CT imaging to contrast placental histological patterning between the preconception treatments. F1 hybrid placentae exhibit larger placental weights than pure C57Bl/6 J offspring but display a proportionally smaller junctional zone with increased glycogen content. The male F1 hybrid offspring of alcohol-exposed sires exhibit modest placental hyperplasia but, unlike pure C57Bl/6 J offspring, do not display observable changes in placental histology, glycogen content, or measurable impacts on fetal growth. Although F1 hybrid female offspring do not exhibit any measurable alterations in fetoplacental growth, RT-qPCR analysis of placental gene expression reveals increased expression of genes participating in the antioxidant response. The reduced placental junctional zone but increased glycogen stores of 129S1/SvImJ-C57Bl/6 J F1 hybrid placentae ostensibly attenuate the previously observed placental patterning defects and fetal growth restriction induced by paternal alcohol use in the C57Bl/6 J strain.</p></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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