纳米二氧化硅对肺组织的跨代影响及其机制。

IF 2.8 4区 医学 Q2 REPRODUCTIVE BIOLOGY
Jiangliang Chu , Chengzhi Liu , Shuang Chen , Yifan Yang , Keyu Zhang , Yiping Fu , Beilei Yuan , Zhe Wang
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引用次数: 0

摘要

纳米材料,如纳米sio2,在各个领域得到了广泛的研究和应用。然而,人们对纳米sio2的跨代效应知之甚少。本研究旨在探讨父亲接触纳米二氧化硅对雄性后代肺部的跨代影响及其机制。将C57BL/6雄性小鼠暴露于纳米sio2环境下,与未暴露的雌性小鼠交配,产生F1代和F2代。通过测定肺功能、肺组织病理、分析肺组织中印迹基因表达和DNA甲基化谱,在两代(F0和F2)中评估纳米sio2的影响。结果表明,潮气量(VT)、1/2潮气量流量(EF50)、呼气峰流量(PEF)、吸气峰流量(PIF)和分气量(MV)在F0代和F2代均显著增加。肺组织HE染色显示F0炎症。肺组织PCR结果提示Dlk1和Dio3的表达有明显变化。此外,纳米sio2暴露增加了Dlk1和Dio3在F0和F2代之间的甲基化水平。综上所述,纳米二氧化硅暴露通过调节Dlk1-Dio3印迹基因的表观遗传调控,对雄性小鼠的肺呼吸功能产生不利影响,可能导致跨代风险。然而,需要进一步的研究来确定成年期的长期生理后果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transgenerational effects and mechanisms of nano-SiO2 on pulmonary
Nanomaterials, such as nano-SiO2, have been extensively studied and applied in various fields. However, little is known about the transgenerational effects of nano-SiO2. The purpose of this study was to investigate the transgenerational effects and mechanisms of paternal nano-SiO2 exposure on the lung of male offspring. Male C57BL/6 mice were exposed to nano-SiO2, which were then mated with unexposed females to produce F1 and F2 generations. The effects of nano-SiO2 were evaluated over two generations (F0 and F2) by determining pulmonary function, lung tissue pathology, and analyzing the imprinted gene expression and DNA methylation profile in lung tissues. The results showed that tidal volume (VT), 1/2 tidal volume flow (EF50), peak expiratory flow (PEF), peak inspiratory flow (PIF), and minute ventilation (MV) increased significantly in both the F0 and F2 generations. Moreover, HE staining of lung tissues showed inflammation of the F0. PCR results of lung tissues imply a significant change in the expression of Dlk1 and Dio3. Furthermore, nano-SiO2 exposure increased the methylation levels of Dlk1 and Dio3 across generations F0 and F2. Overall, nano-SiO2 exposure detrimentally impacts lung respiratory function in male mice by modulating the epigenetic regulation of Dlk1-Dio3 imprinted genes, potentially leading to transgenerational risks. However, further studies are needed to determine the long-term physiological consequences in adulthood.
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来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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