Reproductive toxicology最新文献

{"title":"Blastocyst exposure to plastic during mice in vitro fertilization impacts placental development","authors":"Claude Saint-Ruf,&nbsp;Yasmine Boumerdassi,&nbsp;Franck Kouakou,&nbsp;Jean-Philippe Wolf,&nbsp;Florence Eustache,&nbsp;Daniel Vaiman,&nbsp;Francisco Miralles","doi":"10.1016/j.reprotox.2025.108856","DOIUrl":"10.1016/j.reprotox.2025.108856","url":null,"abstract":"<div><h3>Introduction</h3><div>Pregnancies from Assisted Reproductive Technologies (ARTs) are associated with a significant prevalence of maternal, neonatal and long-term adverse health issues. These anomalies are generally attributed to the <em>in vitro</em> manipulations involved in these procedures. Concerns have been raised on the quality of the culture media, however the potential influence of the chemical composition of the devices used in the in vitro fertilization (IVF) has been poorly analysed. By comparing the transcriptomes of placentas from mouse blastocysts obtained by IVF on plasticware, glassware and naturally conceived, we have previously established that plasticware profoundly impacts placental development.</div></div><div><h3>Methods</h3><div>Transcriptomics, transcriptome deconvolution analysis, Gene Set Enrichment Analysis.</div></div><div><h3>Results</h3><div>Plasticware alters placental gene expression mostly in the trophoblast compartment, and alters cell composition favouring Glycogen Cells. These modifications correlate with alterations of epigenetic mechanisms (alterations of imprinted genes, microRNAs expression, methylation alterations). Also, sex-stratified analysis reveals that these effects are more drastic in female than male placentas. The effect of glassware on the transcriptome and cellular composition of the placenta is milder, and in particular has lower impact on the imprinted gene or microRNAs expression.</div></div><div><h3>Conclusion</h3><div>In vitro culture in plasticware during IVF procedures sex-specifically alters gene expression and/or cell composition in the placenta, possibly through factors released by the plasticware having an action on epigenetic actors (imprinted genes, miRNAs and DNA methylation).</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108856"},"PeriodicalIF":3.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucoxanthin mitigates mercury-induced mitochondrial toxicity in the human ovarian granulosa cell line","authors":"Ekramy M. Elmorsy ,&nbsp;Huda A. Al Doghaither ,&nbsp;Ayat B. Al-Ghafari ,&nbsp;Saad Amer ,&nbsp;Manal S. Fawzy ,&nbsp;Eman A. Toraih","doi":"10.1016/j.reprotox.2025.108855","DOIUrl":"10.1016/j.reprotox.2025.108855","url":null,"abstract":"<div><div>Mercury (Hg) is known to be a hazardous toxin with a significant negative impact on female reproduction through mechanisms that remain unclear. The carotenoid fucoxanthin (FX) is an antioxidant with several positive effects on human health. This study aimed to examine the potential protective role of FX in reducing the Hg-induced bioenergetic disturbances in a human ovarian granulosa cell line model. (methods briefly) Hg was found to reduce the viability of granulosa cells in a concentration-dependent manner, with an estimated 72-hour EC50 of 10 µM. In contrast, FX (10 and 20 µM) improved cell viability. Hg (1 and 10 µM) significantly reduced cellular ATP levels, mitochondrial membrane potential, oxygen consumption rates, and lactate production. Additionally, Hg impaired the activities and kinetics of mitochondrial complexes I and III and reduced the expression of mitochondrial genes ND1, ND5, cytochrome B, cytochrome C oxidase, and ATP synthase subunits 6 and 8. According to tests on mitochondrial membranes, Hg increased membrane fluidity by reducing saturated fatty acid levels and increasing those of unsaturated fatty acids. Hg also promoted mitochondrial swelling and enhanced the inner mitochondrial membrane permeability to hydrogen and potassium ions. FX (10 µM) was shown to mitigate the negative effects of Hg on the viability of treated granulosa cells, bioenergetics parameters, and mitochondrial membrane integrity in a concentration-dependent manner. Based on these findings, bioenergetic disruption may be a key underlying cause of Hg-induced ovarian dysfunction. Furthermore, FX may have a potential therapeutic role in treating ovarian disorders caused by Hg-induced disruption of granulosa cell bioenergetics.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108855"},"PeriodicalIF":3.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal exposure to an environmentally relevant phthalate mixture alters oxidative stress, apoptosis, cell cycle regulators, and steroidogenic factors in the ovaries of F1 mice.","authors":"Endia J Fletcher, Winter S Stubblefield, Taylor A Seaton, Adira M Safar, Angela E Dean, Mary J Laws, Emily Brehm, Jodi A Flaws","doi":"10.1016/j.reprotox.2025.108858","DOIUrl":"https://doi.org/10.1016/j.reprotox.2025.108858","url":null,"abstract":"<p><p>Phthalates are synthetic chemical compounds found in consumer products and known endocrine-disrupting chemicals. However, it is not well known if prenatal exposure to phthalate mixtures can affect reproductive health in female offspring. Thus, this study tested the hypothesis that prenatal exposure to an environmentally relevant phthalate mixture disrupts long-term ovarian function in adult F1 mice. Pregnant CD-1 dams were dosed orally with vehicle control (corn oil) or phthalate mixture (20μg/kg/day-500 mg/kg/day) from gestational day 10 until birth. After birth, the F1 female ovaries and sera were collected on postnatal day (PND) 60, 3 months, and 6 months. F1 ovaries were used for evaluation of the proliferative marker, Ki67, and to quantify gene expression of steroidogenic regulators, antioxidant enzymes, apoptotic factors and cell cycle regulators. Sera were collected to measure sex steroid hormone levels. At PND60, prenatal exposure to the mixture decreased the expression of Star, Cyp11a1, Bad, and Casp3 in F1 females at PND60 compared to controls. At 3 months, the mixture decreased expression of Cyp11a1, Hsd3b1, Sod1, Casp3, Casp8, and Fas and increased gene expression of Star and Gpx in F1 ovaries compared to the controls. At 6 months, the mixture decreased testosterone levels and expression of Gsr, Bad, Bok, Casp8, Fas and Traf3, and it increased expression of Star in F1 females compared to the controls. Collectively, these data suggest that the prenatal exposure to an environmentally relevant phthalate mixture may have long-term consequences on ovarian health and function in F1 females long after initial exposure.</p>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":" ","pages":"108858"},"PeriodicalIF":3.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between maternal exposure to air pollution and intrapartum fetal distress: A retrospective cohort study","authors":"Alireza Khajavi ,&nbsp;Ehsan Zahmatkesh ,&nbsp;Maedeh Raznahan ,&nbsp;Ali Shafaghat ,&nbsp;Amir Hussein Noohi ,&nbsp;Mohammad E. Khamseh ,&nbsp;Laily Najafi ,&nbsp;Farid Zayeri","doi":"10.1016/j.reprotox.2025.108860","DOIUrl":"10.1016/j.reprotox.2025.108860","url":null,"abstract":"<div><div>This study aimed to investigate the association between maternal exposure to ambient air pollution and intrapartum fetal distress. The retrospective data were obtained for 150 parturients, ages 19–45, referred to Kamali Teaching Hospital, Karaj, Iran, in 2016. To assess the impact of the 2, 4, and 8 weeks exposure windows before the delivery of particulate matter ≤ 10 micrometers (PM₁₀) and sulfur dioxide (SO₂) on fetal distress incidence, logistic regression models were fitted, crudely and adjusted for maternal covariates. The parturients' ages owned a mean (standard deviation) of 30.4 (5.4). Moreover, 17 fetal distress cases were detected (11.3 %), demonstrating higher proportions of cousin marriage and family history of diabetes than the non-fetal distress group. Adjusted for body mass index, cousin marriage, abortion, and family history of diabetes, and over the eight weeks exposure window, a five µg/m³ increase of SO₂ and PM₁₀ provided odds ratios of 2.12 (95 % CI: 1.04–4.30) and 1.61 (95 % CI: 1.08–2.40), respectively, for fetal distress incidence. To conclude, we found the long-term impacts of SO₂ and PM₁₀ on the incidence of fetal distress based on the exposure level during the last eight weeks of pregnancy.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108860"},"PeriodicalIF":3.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental exposure to an environmentally relevant dose of Bisphenol S impairs postnatal growth and disrupts placental transcriptional profile in female rat","authors":"J. Fudvoye ,&nbsp;D. Lopez-Rodriguez ,&nbsp;C. Glachet ,&nbsp;D. Franssen ,&nbsp;Q. Terwagne ,&nbsp;A. Lavergne ,&nbsp;A.F. Donneau ,&nbsp;C. Munaut ,&nbsp;P. Dehan ,&nbsp;A. Lomniczi ,&nbsp;A.S. Parent","doi":"10.1016/j.reprotox.2025.108854","DOIUrl":"10.1016/j.reprotox.2025.108854","url":null,"abstract":"<div><div>Because of its possible adverse effects on human health and its ubiquitous nature, Bisphenol A (BPA) is gradually being replaced by presumably safer alternatives like Bisphenol S (BPS). However, data regarding the effects of developmental exposure to BPS on pregnancy and fetal outcomes are very scarce. Here we show that perinatal exposure to BPS at a very low dose significantly impairs postnatal growth and affects the placental transcriptome in rats. Oral exposure one week before mating and during gestation and lactation to a very low dose of BPS (25 ng/kg/day) is associated with impaired postnatal growth without significant difference in fetal weight on gestational day 18 in females. In contrast, in males, exposure to BPS 25 decreased fetal weight on gestational day 18 but growth restriction did not persist into adulthood. In female, exposure to this very low dose of BPS decreased the placental mRNA expression of fucosyltransferase2 (<em>Fut2</em>), pregnancy-specific glycoprotein 22 (<em>Psg22</em>), Wnt family member 7b (<em>Wnt7b</em>) which are involved in early placental development. Placental DNA methylation of steroid receptor coactivator 2 (<em>src2</em>), a key mediator of steroid induced decidualization, was significantly reduced, while placental <em>src2</em> mRNA expression was unaffected. These results suggest that early exposure to a very low dose of BPS has long term consequences on growth trajectory and is associated with placental dysregulation.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108854"},"PeriodicalIF":3.3,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sub-chronic exposure to Kalach on male reproductive system and sperm function: In silico modelling and in vivo study in rats","authors":"Marwa Ben Amor ,&nbsp;Latifa Hamdaoui ,&nbsp;Salima Daoud ,&nbsp;Mariem Ammar ,&nbsp;Nour Louati ,&nbsp;Aida Elleuch ,&nbsp;Riadh Badraoui ,&nbsp;Lobna Ben Mahmoud ,&nbsp;Ikram Ben Amor ,&nbsp;Afifa Sellami ,&nbsp;Tarek Rebai","doi":"10.1016/j.reprotox.2025.108853","DOIUrl":"10.1016/j.reprotox.2025.108853","url":null,"abstract":"<div><div>Kalach 360 SL (KL), a glyphosate-based herbicide, is among the most widely used herbicides in Tunisia. This study aimed to evaluate the impact of sub-chronic exposure to KL on the male reproductive system and sperm parameters in adult rats after one and two cycles of spermatogenesis. 15 rats were randomly divided into three groups: a control group (G1) and two experimental groups (G2 and G3), exposed to KL at a dose of 102.2 mg/kg each day for 48 days. Treated groups G2 and G3 were sacrificed at day 48 and at day 96, respectively. We measured serum levels of testosterone and oestradiol, oxidative stress markers in testis, epididymal sperm parameters, sperm mitochondrial membrane potential (MMP), as well as testicular histopathology and morphometry as diagnostic markers of reproductive dysfunction. Additionally, we complemented the in vivo study with in silico modelling. Kl impaired sperm parameters, altered MMP, promoted oxidative stress, and affected testicular morphology, leading to reduced seminiferous epithelium height and delayed spermatogenesis arrest. KL caused significant declines in serum testosterone levels after 48 days (G2 group), supporting the herbicide's anti-androgenic activity. Notably, following cessation of exposure, testosterone levels increased and sperm concentration returned to normal by day 96 (G3 group). The computational approach revealed that glyphosate binds to the androgen receptor (2Q7K and 3QKM) with good affinities and strong molecular interactions, corroborating the in vivo results. We conclude that KL may interfere with spermatogenesis, impair male fertility, and function as a potential endocrine disruptor with anti-androgenic activity.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108853"},"PeriodicalIF":3.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small extracellular vesicles derived from Nrf2-stimulated bone marrow mesenchymal stem cells ameliorated the testis damage and fertility disorder in doxorubicin-treated mice","authors":"Maryam Taher,&nbsp;Hanieh Jalali,&nbsp;Homa Mohseni Kouchesfehani","doi":"10.1016/j.reprotox.2025.108847","DOIUrl":"10.1016/j.reprotox.2025.108847","url":null,"abstract":"<div><div>Bone marrow mesenchymal/stromal stem cell (BMSC)-derived small extracellular vesicles (sEVs) are promising therapeutic agents owing to their low immunogenicity and ability to cross biological barriers. Doxorubicin (DOX), a common chemotherapeutic agent, damages testicular tissue. This study aimed to enhance the antioxidant activity of sEVs by activating the <em>Nrf2</em> gene in BMSCs and evaluate their therapeutic potential for DOX-induced fertility disorders. Testicular damage was induced by DOX in NMRI mice. BMSCs from Wistar rats were treated with Bardoxolone methyl (BaMet) to upregulate <em>Nrf2</em>. The sEVs were isolated through differential ultracentrifugation and validated for size, morphology, and protein expression. The antioxidant activity was assessed using specific kits. sEVs containing 10 μg of proteins were injected intravenously into DOX-injured mice. After 35 days, the testes were collected for histopathological, hormonal, and immunological analyses, along with the evaluation of sperm parameters. Male and female mice were paired to determine the pregnancy rates. BaMet-sEVs exhibited higher antioxidant activity and significantly improved serum testosterone levels, testicular cell populations, sperm viability, and motility in DOX-injured mice. In addition, BaMet-sEVs treatment enhanced fertility and increased the number of offspring. This study demonstrated the effectiveness of BaMet-sEVs in mitigating DOX-induced testicular damage.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108847"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single- and combined-heavy metals/metalloids exposures are associated with infertility in US women aged 20–44: NHANES 2013–2020 analysis","authors":"He-Bin Chi ,&nbsp;Jia-Jia Tang ,&nbsp;Xiao-Yuan Fan ,&nbsp;Han-Wen Zhang ,&nbsp;Feng Tang ,&nbsp;Xian-Shu Lin ,&nbsp;Bing-Rui Yang ,&nbsp;Na Li ,&nbsp;Jun Guo ,&nbsp;Li-An-Sheng Wu ,&nbsp;Qiu-Qi Huang ,&nbsp;Yin-Yin Xia","doi":"10.1016/j.reprotox.2025.108851","DOIUrl":"10.1016/j.reprotox.2025.108851","url":null,"abstract":"<div><div>Infertility is a major medical and social issue, with environmental factors, including metal exposure, playing a crucial role. This study analyzes how individual metals and their mixtures, which include a selection of heavy metals and metalloids totaling sixteen metals, contribute to infertility risk, using data from the National Health and Nutrition Examination Surveys. The study included 1326 women aged 20–44 years, comprising 1145 classified as fertile and 181 as infertile, with data on reproductive questionnaires and covariates. Infertility was defined through self-reported data. To assess the associations between exposure to these elements and infertility risk, we employed logistic regression, principal component analysis (PCA), restricted cubic splines (RCS), quantile regression with group-specific combination (qgcomp), and bayesian kernel machine regression (BKMR). After adjusting for potential confounders, logistic regression revealed positive associations of blood manganese (BMn) and urinary tin (USn) with infertility, whereas serum selenium (SSe) was negatively associated. RCS analysis demonstrated nonlinear relationships between urinary barium (UBa), urinary molybdenum (UMo), and urinary antimony (USb) and infertility. Potential interactions were identified between the following metal pairs: UMo and urinary cadmium, USb and UBa, and USb and UMo. PCA identified a positive association between PC3 and infertility (OR = 1.17, 95 % CI: 1.00, 1.36). The qgcomp model also indicated a positive association between metal mixtures and female infertility (OR = 1.25, 95 % CI: 1.03, 1.52). In conclusion, this study highlights significant associations between exposure to specific metals and infertility risk among women of reproductive age.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108851"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of air pollution on sperm DNA methylation","authors":"Jordan L. Moore ,&nbsp;Seth J. Parks ,&nbsp;Emma R. James ,&nbsp;Kenneth I. Aston ,&nbsp;Timothy G. Jenkins","doi":"10.1016/j.reprotox.2025.108850","DOIUrl":"10.1016/j.reprotox.2025.108850","url":null,"abstract":"<div><div>A number of environmental factors have been shown to impact the sperm epigenome. Air pollution is one of the largest health and environmental hazards in the world today and has been implicated in many modern diseases. Recently, air pollution has been shown to alter methylation signatures in some body tissues, indicating that air pollution may also affect the sperm epigenome. The present experiment was conducted to analyze how seasonal air pollution in the Salt Lake Valley may impact DNA methylation patterns in sperm and to establish a relationship between air pollution and sperm epigenetic health as measured by DNA methylation. Sperm DNA methylation patterns were assessed in 74 individuals, who presented at the University of Utah Andrology Clinic for semen analysis, using the Illumina Human MethylationEPIC BeadChip array. Each semen sample collected, as per the fifth edition of WHO reference values for human semen characterization, was deemed normal. Two sample groups from the Salt Lake Valley, Urban Winter (UW, n = 20), Urban Summer (US, n = 21), and two sample groups east of the Wasatch mountains, Rural Winter (RW, n = 19) and Rural Summer (RS, n = 14), were compared to assess the effect of air pollution on sperm DNA methylation patterns. Due to seasonal inversions, urban winters are characterized by increased air pollution compared to summer months. Therefore, the UW sample group was designated as treatment and the three remaining groups (US, RW, RS) were designated as control. We conducted multiple differential methylation analyses using a sliding window approach which utilized the USeq software package. A sliding window analysis of UW versus US was conducted first, followed by a confirmatory analysis comparing UW versus RW and RS. Outputs from the USeq analysis were assessed using several tools including the Stanford GREAT analysis and an analysis of methylation instability at key promoter regions in sperm. The sliding window analysis identified six differentially methylated regions (DMRs) between the UW and US groups (Wilcoxon FDR ≥ 40, corresponding p-value of ∼0.0001). Three of these six regions were confirmed with the second confirmatory analysis of UW versus RS/RW (Wilcoxon FDR ≥ 20, p-value&lt;0.01). According to a GREAT analysis, each of the identified regions exhibited multiple gene ontology associations. Air pollution subtly alters DNA methylation in sperm, indicating that certain regions of the sperm epigenome may be susceptible to air pollution-induced modification with possible implications for reproductive and offspring health.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108850"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teratogenic effect evaluation of Monascus red oral exposure to pregnant rats and their gut microbiota","authors":"Yuenan Wang ,&nbsp;Xuedan Xu ,&nbsp;Yun Liu ,&nbsp;Zhenfeng Huang ,&nbsp;Hongxia Wang ,&nbsp;Kexin Wang ,&nbsp;Yayi Huang ,&nbsp;Xinyu Yang ,&nbsp;Tingting Sun ,&nbsp;Jieling Wang ,&nbsp;Jianbin Tan ,&nbsp;Xingfen Yang ,&nbsp;Min Zhao","doi":"10.1016/j.reprotox.2025.108843","DOIUrl":"10.1016/j.reprotox.2025.108843","url":null,"abstract":"<div><div><em>Monascus</em> red (MR) is widely used as a natural food colorant and preservative in East Asia. However, the potential effects of MR during pregnancy remains unknown. In this study, MR was administrated to Sprague-Dawley (SD) rats at doses of 0, 0.50, 1.58, and 5.00 g/kg bw on gestational days 6–15 by oral gavage. In the maternal and embryo-fetal examinations, there were no marked toxicities in terms of general clinical signs, body weight, food consumption, serum endocrine indices, organ weights, thyroid histopathology, examinations of uterine contents and fetuses. In the gut microbiota analysis, the 5.00 g/kg bw dose of MR decreased the α diversity and slightly changed their community structure at the genus level. Yet no marked toxicities in maternal animals or embryo-fetal development were observed. The no-observed-adverse-effect-level (NOAEL) of the maternal and developmental toxicity through oral exposure to MR was 5.00 g/kg bw, the highest dose tested in rats.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"132 ","pages":"Article 108843"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}