Jiangliang Chu , Chengzhi Liu , Shuang Chen , Yifan Yang , Keyu Zhang , Yiping Fu , Beilei Yuan , Zhe Wang
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引用次数: 0
Abstract
Nanomaterials, such as nano-SiO2, have been extensively studied and applied in various fields. However, little is known about the transgenerational effects of nano-SiO2. The purpose of this study was to investigate the transgenerational effects and mechanisms of paternal nano-SiO2 exposure on the lung of male offspring. Male C57BL/6 mice were exposed to nano-SiO2, which were then mated with unexposed females to produce F1 and F2 generations. The effects of nano-SiO2 were evaluated over two generations (F0 and F2) by determining pulmonary function, lung tissue pathology, and analyzing the imprinted gene expression and DNA methylation profile in lung tissues. The results showed that tidal volume (VT), 1/2 tidal volume flow (EF50), peak expiratory flow (PEF), peak inspiratory flow (PIF), and minute ventilation (MV) increased significantly in both the F0 and F2 generations. Moreover, HE staining of lung tissues showed inflammation of the F0. PCR results of lung tissues imply a significant change in the expression of Dlk1 and Dio3. Furthermore, nano-SiO2 exposure increased the methylation levels of Dlk1 and Dio3 across generations F0 and F2. Overall, nano-SiO2 exposure detrimentally impacts lung respiratory function in male mice by modulating the epigenetic regulation of Dlk1-Dio3 imprinted genes, potentially leading to transgenerational risks. However, further studies are needed to determine the long-term physiological consequences in adulthood.
期刊介绍:
Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine.
All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.