Effects of non-nutritive sweeteners on the sweet taste receptor expression and reproductive function in female guinea pigs

IF 2.8 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2025-07-11 DOI:10.1016/j.reprotox.2025.108998

Shanli Zhu , Ziqing Li , Fangxiong Shi , Junrong Li

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引用次数: 0

Abstract

Non-nutritive sweeteners (NNS) are widely used food additives with unclear reproductive effects. This study examined how dietary NNS supplementation affects taste receptors T1R2/T1R3 expression in female guinea pig reproductive organs. Four groups of ten animals either received the control (basal diet) or basal diet supplemented with either rebaudioside A (RA, 330 mg/kg), saccharin sodium (SS, 800 mg/kg), or sucralose (TGS, 167 mg/kg) for 28 consecutive days. All NNS significantly increased serum progesterone and estradiol levels (P < 0.05). RA significantly upregulated ovarian T1R2/T1R3 expression (P < 0.05), while SS and TGS groups showed higher incidence of follicular cysts and structural disorganization. In uterine tissues, T1R2 expression was significantly higher in TGS and SS groups compared to control and RA groups (P < 0.05), while T1R3 expression was significantly elevated in the SS group compared to all others (P < 0.05). Our findings suggest that dietary NNS supplementation induces ovarian histological alterations, activates sweet taste receptors in reproductive tissues, and elevates serum P₄ and E₂ levels, indicating potential risks to female reproductive health through sweet taste receptor-mediated modulation of steroidogenesis and disruption of follicular development.

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非营养性甜味剂对雌性豚鼠甜味受体表达和生殖功能的影响

非营养性甜味剂（NNS）是一种应用广泛的食品添加剂，其生殖效应尚不清楚。本研究考察了膳食中添加NNS对雌性豚鼠生殖器官味觉受体T1R2/T1R3表达的影响。4组动物每10只饲喂对照组（基础饲粮）或在基础饲粮中添加雷鲍迪苷A （RA, 330 mg/kg）、糖精钠（SS, 800 mg/kg）或三氯蔗糖（TGS, 167 mg/kg），连续饲喂28 d。所有NNS均显著提高血清孕酮和雌二醇水平（P <； 0.05）。RA显著上调卵巢T1R2/T1R3表达（P <； 0.05），而SS和TGS组卵泡囊肿和结构紊乱发生率更高。在子宫组织中，T1R2在TGS和SS组的表达明显高于对照组和RA组（P <； 0.05），而T1R3在SS组的表达明显高于其他各组（P <； 0.05）。我们的研究结果表明，饮食中添加NNS可诱导卵巢组织学改变，激活生殖组织中的甜味受体，提高血清中硫酸钾和e2水平，表明甜味受体介导的类固醇生成和卵泡发育的调节可能对女性生殖健康造成潜在风险。

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.