Regenerative medicine最新文献

{"title":"Toward improved AAV gene therapies for retinal disorders: challenges and advances.","authors":"Oliver Siontas, Mika Brown, Seungkuk Ahn","doi":"10.1080/17460751.2025.2544497","DOIUrl":"10.1080/17460751.2025.2544497","url":null,"abstract":"<p><p>Adeno-associated virus (AAV) vectors have transformed the landscape of in vivo gene therapy, with retinal diseases emerging as a major area of progress. The eye offers unique advantages as a therapeutic target: it is accessible, compartmentalized, and relatively immune-privileged, allowing localized delivery with reduced systemic effects. The landmark 2017 approval of the first AAV-based gene therapy for an inherited retinal disorder sparked a surge of clinical trials using AAV vectors - underscoring their potential for treating genetic eye diseases. However, challenges remain, including AAV's limited capacity for large genes, suboptimal precision in cell-type-specific targeting, and inefficient transduction of certain retinal cells via minimally invasive routes. In response, researchers are engineering next-generation AAV capsids, optimizing gene expression cassettes, developing novel delivery strategies, and advancing tissue and organoid-based screening platforms. This article highlights these efforts as essential to overcoming current barriers in retinal AAV gene therapy.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"299-303"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144795237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Migrasomes: a promising extracellular vesicle-like novel organelle for bone regeneration.","authors":"Chaoting Yan, Yueguang Gu, Kunpeng Wang, Geng Wu","doi":"10.1080/17460751.2025.2546213","DOIUrl":"10.1080/17460751.2025.2546213","url":null,"abstract":"<p><p>Bone regeneration represents a key objective in bone tissue engineering and involves a series of coordinated biological processes, including immunomodulation, neuroregulation, angiogenesis, and osteogenesis. Recent studies have underscored the therapeutic potential of extracellular vesicles (EVs) in promoting osteogenesis and facilitating the repair of bone defects, supporting their application as a promising cell-free strategy in regenerative medicine. Migrasomes, vesicle-like organelles anchored to retraction fibers and first identified in 2015, have emerged as key mediators in intercellular communication, lateral transfer of mRNA and proteins, and mitochondrial homeostasis. Through cell-free treatment, these functions support the activity and regenerative ability of stem cells during bone repair. This review provides an updated overview of migrasome-related research, emphasizing their roles in molecular delivery and regulation during bone regeneration. Moreover, the potential of migrasomes as innovative tools for bone tissue engineering is discussed, along with prospective strategies to enhance their utility through advances in understanding their biogenesis and cargo sorting. Despite their known biological functions, the therapeutic applications of migrasomes in bone regeneration remain largely unexplored, highlighting the need for further investigation in this emerging field.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"305-316"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Industry updates from the field of stem cell research and regenerative medicine in March 2025.","authors":"Dusko Ilic, Mirjana Liovic","doi":"10.1080/17460751.2025.2493446","DOIUrl":"10.1080/17460751.2025.2493446","url":null,"abstract":"<p><p>Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions in March 2025.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"275-283"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The eggshell membrane as a barrier membrane for guided bone regeneration.","authors":"Faisal F Alotaibi, Zainab M AlFaltawi, Sharon R Oyhanart, Jonathan C Knowles, Francesco D'Aiuto, David Y S Chau","doi":"10.1080/17460751.2025.2542056","DOIUrl":"10.1080/17460751.2025.2542056","url":null,"abstract":"<p><strong>Background: </strong>The eggshell membrane (ESM) is a natural resource with a distinct design and composition, offering structural features consistent with barrier membranes used in guided bone regeneration (GBR), making it a promising candidate for this application. This study aims to assess the feasibility of chicken and duck ESMs as GBR alternatives by comparing them with resorbable porcine-derived collagen (Porcine CM, Bio-Gide®) and non-resorbable dense polytetrafluoroethylene (d-PTFE, Cytoplast^TM).</p><p><strong>Methods: </strong>ESMs were extracted using a standardized protocol. Membranes were then analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), contact angle measurements (CAM), and cell culture-based assays.</p><p><strong>Results: </strong>FTIR revealed similar collagen spectra among membranes. SEM showed structural similarities between ESMs and Bio-Gide. DSC indicated integrity maintenance at 37°C and varied storage conditions. CAM testing demonstrated collagen-based membranes' higher hydrophilicity compared to d-PTFE. DMA analysis showed duck ESM's superior tensile strength and Young's modulus compared to chicken ESM and porcine CM. Biological evaluation revealed high compatibility with human gingival fibroblasts for all materials.</p><p><strong>Conclusion: </strong>Findings illustrate a novel sustainable biomaterial that could be utilized for GBR and other periodontal therapies, particularly in its capacity to function as a physical barrier consistent with the traditional role of GBR membranes.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"285-298"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the immune system and developing targeted vaccines: overall strategies of leveraging the body’s natural defense mechanisms to enhance cancer treatment","authors":"Abid H. Banday","doi":"10.4103/regenmed.regenmed-d-25-00011","DOIUrl":"https://doi.org/10.4103/regenmed.regenmed-d-25-00011","url":null,"abstract":"The immune system serves as a pivotal defense mechanism against foreign materials and abnormal cells, including cancer. Cancer immunotherapy exploits the immune system’s potential to target and eliminate malignant tumor cells, either through immunization or therapeutic antibodies. The presence of unusual antigens, such as GD2 and ErbB2, provides specific targets for immunotherapy. Next-generation immunotherapy, combining antibodies with immune-stimulatory cytokines, presents a promising avenue to enhance localized immune responses against tumors while minimizing systemic side effects. Challenges in cancer vaccine development include the diversity of tumor cells and their similarity to normal cells. Recent decades have seen significant progress in cancer vaccine development, with US Food and Drug Administration-approved therapeutic vaccines like Sipuleucel-T for prostate cancer and BCG for bladder cancer. However, challenges related to reproducibility and efficacy in cancer subjects persist. The current landscape of cancer treatment is undergoing a transformative shift, with combination therapies incorporating cytokines, tumor-associated antigens, and monoclonal antibodies becoming integral to treatment strategies. This dynamic approach reflects ongoing efforts to overcome challenges in developing effective therapeutic cancer vaccines and underscores the commitment to advancing the field. Advances in genomics allow for the development of personalized cancer vaccines tailored to an individual’s unique tumor profile, addressing the challenge of intratumoral heterogeneity. Combining cancer vaccines with other immunotherapies, such as immune checkpoint inhibitors, may enhance the overall anti-cancer immune response. Identifying and targeting neoantigens (mutated proteins specific to cancer cells) offers a more precise and selective approach to cancer vaccine development. These evolving strategies emphasize leveraging the body’s natural defense mechanisms to enhance the overall efficacy of cancer treatment, marking a departure from traditional approaches like surgery, chemotherapy, and radiation.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":"2 4","pages":"185-192"},"PeriodicalIF":0.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coagulation activation and hepatic engraftment of human amnion-derived cells after intraportal transplantation in rats.","authors":"Miyako Tanaka, Kazuaki Tokodai, Kaoru Okada, Masato Sato, Hitomi Okita, Takako Ito, Tetsuro Hoshiai, Masatoshi Saito, Toshio Miki, Michiaki Unno, Takashi Kamei, Masafumi Goto","doi":"10.1080/17460751.2025.2526915","DOIUrl":"10.1080/17460751.2025.2526915","url":null,"abstract":"<p><strong>Aims: </strong>Human amniotic epithelial cells (hAECs) have emerged as a promising cell source for regenerative medicine, with intraportal transplantation as a potential delivery route. However, the extent to which hAECs induce an immediate inflammatory response remains unclear. This study investigated tissue factor (TF) expression in isolated hAECs and assessed coagulation activation following intraportal transplantation in a rat model.</p><p><strong>Materials and methods: </strong>TF expression was analyzed before and after cryopreservation, while thrombin - antithrombin (TAT) complex levels were measured to evaluate coagulation activation. To assess engraftment and hepatocyte-like function, hAECs were transplanted into non-albuminemic rats, followed by serial measurements of serum human albumin levels and histological liver analysis.</p><p><strong>Results: </strong>The findings indicate that hAECs express TF pre- and post-cryopreservation. Intraportal transplantation resulted in a significant increase in plasma TAT levels, which was mitigated by heparin administration. Furthermore, human albumin levels increased post-transplantation, and albumin-positive cells were detected in the liver on day 21.</p><p><strong>Conclusion: </strong>These results suggest that intraportal hAEC transplantation is a feasible approach for hepatic engraftment; however, TF expression may trigger coagulation activation, potentially leading to an instant blood-mediated inflammatory reaction. Further research is warranted to optimize anticoagulation strategies and evaluate long-term engraftment efficacy for clinical applications.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"233-242"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing stem cells for corneal disease: recent developments and future perspectives.","authors":"Ansu Ann John, Hooman Ahmadzadeh, Alfonso L Sabater","doi":"10.1080/17460751.2025.2526253","DOIUrl":"10.1080/17460751.2025.2526253","url":null,"abstract":"<p><p>Stem cell-based regenerative therapies have revolutionized ocular medicine, offering unprecedented potential to restore vision and repair damaged corneal tissues. Recent advancements have been driven by the growing demand to overcome corneal donor shortages and the complications associated with corneal transplantation. This paper provides a comprehensive review of clinical trials and regulatory approvals over the past seven years, categorized by target disease. Despite remarkable progress, significant challenges remain in ensuring safety, efficacy, and economic viability, necessitating rigorous characterization, global standardization, and ethical considerations. Future directions include leveraging gene-editing technologies, personalized medicine, and integrated regenerative approaches to optimize therapeutic outcomes.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"257-274"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward equitable regenerative medicine: a health equity research framework for emerging regenerative treatments.","authors":"Mohamed Addani, Lindsay Beaman, Goldie Smith Byrd, Anthony Atala, Zubin Master","doi":"10.1080/17460751.2025.2521963","DOIUrl":"10.1080/17460751.2025.2521963","url":null,"abstract":"<p><p>Regenerative medicine continues to advance rapidly, yet research examining health disparities within this field remains notably limited. The distinctive characteristics of regenerative therapies - complex manufacturing, specialized delivery, and personalized approaches - present unique challenges for equitable health care access beyond traditional disparities. This gap is concerning as many conditions targeted by regenerative treatments disproportionately affect underserved populations. Our analysis of the literature identifies knowledge gaps and demonstrates the critical need for a multi-level approach to health equity research in regenerative medicine. We propose a research framework involving three key steps. First: systematic investigation of field-specific access barriers through established health disparity frameworks, focusing on factors uniquely relevant to regenerative therapies across individual, interpersonal, organizational, community, and societal levels. Second: development and rigorous evaluation of targeted, multi-level interventions addressing these barriers. Third: implementation of evidence-based strategies using equity-focused frameworks to translate findings into practice, ensuring equity considerations are embedded throughout development and delivery processes. By prioritizing health equity research while many regenerative treatments are still developing, the field has an unprecedented opportunity to create inclusive access pathways and ensure its potentially revolutionary therapies benefits all populations.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"243-256"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current clinical applications of RNA-LNPs in regenerative medicine.","authors":"Md Sadique Hussain, Kavita Goyal, Ajay Singh Bisht, Haider Ali, Gaurav Gupta","doi":"10.1080/17460751.2025.2529070","DOIUrl":"10.1080/17460751.2025.2529070","url":null,"abstract":"","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"229-231"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiomyocyte regeneration: lessons from neonatal hearts.","authors":"Chithra K Pushpan, Finosh G Thankam","doi":"10.1080/17460751.2025.2510087","DOIUrl":"10.1080/17460751.2025.2510087","url":null,"abstract":"","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"165-168"},"PeriodicalIF":3.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}