Regenerative medicine最新文献

Leveraging electric current stimulation and chemical induction to enhance MSC differentiation into cardiomyocytes. 利用电流刺激和化学诱导促进间充质干细胞向心肌细胞分化。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-06-06 DOI: 10.1080/17460751.2025.2514905

Yong-Ji Chen, Yu-Hsin Lin, Chi-Hsiang Wu, Jhe-Lun Hu, Lain-Chyr Hwang, Pei-Leun Kang, Shwu-Jen Chang

{"title":"Leveraging electric current stimulation and chemical induction to enhance MSC differentiation into cardiomyocytes.","authors":"Yong-Ji Chen, Yu-Hsin Lin, Chi-Hsiang Wu, Jhe-Lun Hu, Lain-Chyr Hwang, Pei-Leun Kang, Shwu-Jen Chang","doi":"10.1080/17460751.2025.2514905","DOIUrl":"https://doi.org/10.1080/17460751.2025.2514905","url":null,"abstract":"Aims: To investigate whether direct electric current stimulation, when combined with chemical induction, can enhance the cardiomyogenic differentiation of mesenchymal stem cells (MSCs), offering a potential strategy for cardiac regeneration.Materials & methods: Bone marrow-derived MSCs were subjected to short-term microcurrent stimulation (90 seconds) using an electroporation cuvette with voltages of 1-10 V and pulse parameters of 2 ms at 0.5-2 hz. 5-azacytidine (5-aza) was added as a chemical inducer. In vitro analyses included morphological observation, immunofluorescence staining, qPCR, and flow cytometry. In vivo, pretreated MSCs were injected into a rat myocardial infarction model.Results: Electrical stimulation enhanced MSC alignment and upregulated cardiomyocyte-specific markers. Gene and protein expression analyses confirmed enhanced differentiation, especially in the combined treatment group. In vivo transplantation resulted in partial restoration of myocardial architecture, though no significant ventricular wall thickening was observed within four weeks.Conclusions: This study introduces a dual approach combining electrical and chemical cues to promote cardiomyogenic differentiation in MSCs. The use of a simple electroporation cuvette offers a practical and accessible method, with potential translational relevance for future cardiac repair strategies.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering organoids for dental pulp tissue regeneration and functional reconstruction. 用于牙髓组织再生和功能重建的工程类器官。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-06-04 DOI: 10.1080/17460751.2025.2514899

Xian-Hua Gao, Xin-Lu Li, Bing Fan, Wei Fan

{"title":"Engineering organoids for dental pulp tissue regeneration and functional reconstruction.","authors":"Xian-Hua Gao, Xin-Lu Li, Bing Fan, Wei Fan","doi":"10.1080/17460751.2025.2514899","DOIUrl":"https://doi.org/10.1080/17460751.2025.2514899","url":null,"abstract":"Organoids, which replicate the three-dimensional architecture and physiological functions of native organs, have emerged as a groundbreaking tool with significant therapeutic potential for tissue regeneration and functional reconstruction. Despite their broad applications in various fields, research on dental pulp organoids and their use in regenerative therapies remains in its early stages, presenting both opportunities and challenges. To advance the understanding of organoid technology and facilitate its translation into pulp regenerative medicine, this review provided a comprehensive overview of organoids, encompassing their developmental history, self-organization mechanisms, fundamental definitions, and current applications. Building on this foundation, we highlighted recent progress in oral and maxillofacial organoid research, with a particular focus on the construction of dental pulp organoids. Additionally, we systematically summarized the commonly employed construction methods and explored innovative bioengineering strategies that hold promise for future applications. Finally, we critically evaluated the existing challenges in applying organoid technology to pulp tissue regeneration and functional reconstruction, while proposing potential solutions to overcome these barriers. This review aimed to provide valuable insights and inspire further research in this rapidly evolving field.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"1-18"},"PeriodicalIF":2.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose derived stem cell activation by macrophages and tendon fibroblasts. 巨噬细胞和肌腱成纤维细胞激活脂肪源性干细胞。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-05-25 DOI: 10.1080/17460751.2025.2510098

Astia Innis, Ismael Bousso, David Aaron Roberts, Brittany Paige Marshall, Lee Song, Stavros Thomopoulos

{"title":"Adipose derived stem cell activation by macrophages and tendon fibroblasts.","authors":"Astia Innis, Ismael Bousso, David Aaron Roberts, Brittany Paige Marshall, Lee Song, Stavros Thomopoulos","doi":"10.1080/17460751.2025.2510098","DOIUrl":"https://doi.org/10.1080/17460751.2025.2510098","url":null,"abstract":"Aims: Tendon injuries are common, and healing often fails due to an over-exuberant inflammatory response and a lack of regeneration. Inflammatory cells play key roles in these processes, with a balance between classically activated pro-inflammatory M1 macrophages and alternatively activated inflammatory resolving M2 macrophages. Adipose-derived mesenchymal stem cells (ASCs) can dampen the pro-inflammatory effectsof macrophages, promote a regenerative environment, and enhance healing. Therefore, the goal of the study was to understand how ASCs are activated by macrophages in vitro.Methods: In vitro co-culture experiments were carried out with ASCs, macrophages, and tendon fibroblasts. RNA-seq and qRT-PCR were performed to determine expression patterns of activated ASCs.Results: M1 macrophages prompted ASCs to upregulate pro-inflammatory signaling, matrix remodeling, and cytokine production pathways, while downregulating those related to cell adhesion and cell cycle. Conversely, TFs prompted ASCs to upregulate pathways involved in cell cycle and cytoskeleton remodeling, and to downregulate pathways associated with immune cell adhesion, inflammatory mediator production, and protein metabolism.Conclusions: The cell-specific activation profiles indicate a possible switch in ASC paracrine signaling depending on the context, from a pro-inflammatory pattern in response to M1 macrophages to a proliferative pattern in response to TFs. Understanding crosstalk between ASCs, TFs, and macrophages is essential for developing stem cell-based therapeutic strategies.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiomyocyte regeneration: lessons from neonatal hearts. 心肌细胞再生：新生儿心脏的经验教训。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-05-23 DOI: 10.1080/17460751.2025.2510087

Chithra K Pushpan, Finosh G Thankam

引用次数: 0

Industry updates from the field of stem cell research and regenerative medicine in March 2025. 2025年3月来自干细胞研究和再生医学领域的行业更新。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-04-17 DOI: 10.1080/17460751.2025.2493446

Dusko Ilic, Mirjana Liovic

引用次数: 0

Muscle atrophy and fibrosis are attenuated after experimental nerve repair associated with heterologous fibrin biopolymer. 异种纤维蛋白生物聚合物可减轻实验性神经修复后的肌肉萎缩和纤维化。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-04-01 Epub Date: 2025-05-23 DOI: 10.1080/17460751.2025.2507480

Kevin Silva Muller, Felipe Cantore Tiburcio, Rinaldo Jose Ortiz, Rui Seabra Ferreira Junior, Benedito Barraviera, José de Anchieta de Castro E Horta, Selma Maria Michelin Matheus

{"title":"Muscle atrophy and fibrosis are attenuated after experimental nerve repair associated with heterologous fibrin biopolymer.","authors":"Kevin Silva Muller, Felipe Cantore Tiburcio, Rinaldo Jose Ortiz, Rui Seabra Ferreira Junior, Benedito Barraviera, José de Anchieta de Castro E Horta, Selma Maria Michelin Matheus","doi":"10.1080/17460751.2025.2507480","DOIUrl":"10.1080/17460751.2025.2507480","url":null,"abstract":"Background: Neurotmesis leads to neuromuscular junction (NMJ) degeneration, muscle atrophy, and functional loss. While neurorrhaphy is standard, motor recovery is often incomplete. Heterologous fibrin biopolymer (HFB) shows potential as an adjunct, hence, we investigate HFB's late regenerative effects.Material/methods: Twenty adult male Wistar rats (CEUA-FMB 1402/2021) were divided into Control (C), Denervated (D), Neurorrhaphy (N), and Neurorrhaphy + HFB (NB) groups. After 120 days, nerves and muscles were analyzed.Results: NB (1355 ± 170.4) showed more intact axons than C (927 ± 170.4, p = .0026) and N (774 ± 158.2, p = .0002). NMJ morphology in NB was closer to C than N, with increased nAChR alpha-1 (NB vs. N p = .0428; NB vs C p = .0084) and Rapsyn (NB vs. N p = .0130; NB vs C p = .0053) expression. Muscle integrity in NB resembled C, exhibiting less atrophy (area: C vs. N p = .0002; NB vs. N p = .0117; perimeter: C vs. N p = .0002; NB vs. N p = .0114; central nuclei: C vs. N p = .0009; NB vs. N p = .0110) and fibrosis (C vs. N p = .0061; N vs. NB p = .0326) compared to N.Conclusion: HFB associated with neurorrhaphy enhanced muscle and nerve regeneration, attenuating muscle atrophy and fibrosis.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"133-146"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioregenerative autologous scaffold made from bone marrow aspirate concentrate, cancellous bone autograft, platelet-rich plasma, and autologous fibrin to treat non-unions of the femur, humerus, and forearm bones: a case series. 由骨髓抽液浓缩液、松质骨自体移植物、富血小板血浆和自体纤维蛋白制成的生物再生自体支架治疗股骨、肱骨和前臂骨不连：病例系列

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-04-01 Epub Date: 2025-05-20 DOI: 10.1080/17460751.2025.2507504

Dulic Oliver, Abazovic Dzihan, Obradovic Mirko, Vranjes Miodrag, Lalic Ivica, Ninkovic Srdjan, Rasovic Predrag, Bjelobrk Mile, Baljak Branko, Milinkov Milan, Tosic Milan, Sarac Srdjan

{"title":"Bioregenerative autologous scaffold made from bone marrow aspirate concentrate, cancellous bone autograft, platelet-rich plasma, and autologous fibrin to treat non-unions of the femur, humerus, and forearm bones: a case series.","authors":"Dulic Oliver, Abazovic Dzihan, Obradovic Mirko, Vranjes Miodrag, Lalic Ivica, Ninkovic Srdjan, Rasovic Predrag, Bjelobrk Mile, Baljak Branko, Milinkov Milan, Tosic Milan, Sarac Srdjan","doi":"10.1080/17460751.2025.2507504","DOIUrl":"10.1080/17460751.2025.2507504","url":null,"abstract":"Aim: To evaluate the effectiveness of a bioregenerative scaffold created from bone marrow aspirate, cancellous bone autograft, platelet-rich plasma, and autologous fibrin in treating complicated non-unions of the supracondylar femur, humeral shaft, and radius and ulna.Methods & materials: Three patients with non-unions resulting from multiple surgical failures underwent bone stabilization along with the application of a novel bioregenerative scaffold. X-rays and subjective assessments were collected prior to surgery and at 6- and 12-months post-surgery.Results: All non-unions demonstrated healing with adequate callus formation, as confirmed by radiological assessments. By 12 months, all patients were able to resume full weight-bearing activities or regain full range of motion and physical strength without pain. Statistical analysis revealed improvements across all assessment scales compared to pre-surgical values.Conclusion: This approach offers a viable option for treating complex long bone non-unions after multiple surgical interventions.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"123-131"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a gene therapy for Charcot-Marie-Tooth disease: progress and challenges. 开发治疗腓骨肌萎缩症的基因疗法：进展与挑战。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-04-01 Epub Date: 2025-04-12 DOI: 10.1080/17460751.2025.2491257

Marina Stavrou, Alexia Kagiava, Irene Sargiannidou, Kleopas A Kleopa

引用次数: 0

Industry updates from the field of stem cell research and regenerative medicine in February 2025. 2025年2月来自干细胞研究和再生医学领域的行业更新。

IF 2.4 4区医学

Regenerative medicine Pub Date : 2025-03-27 DOI: 10.1080/17460751.2025.2483100

Dusko Ilic, Mirjana Liovic

引用次数: 0

Industry updates from the field of stem cell research and regenerative medicine in January 2025. 2025年1月来自干细胞研究和再生医学领域的行业更新。