Regenerative medicine最新文献_第2页

Combined umbilical cord-derived mesenchymal stem cell exosome and cell therapy in heart failure with reduced ejection fraction. 脐带源性间充质干细胞外泌体和细胞联合治疗心力衰竭伴射血分数降低。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-06-01 Epub Date: 2026-08-24 DOI: 10.1080/17460751.2026.2723032

Berhan Keskin, Beytullah Cakal, Aykun Hakgor, Atakan Dursun, Aysel Akhundova, Umeyir Savur, Ozlem Onder, Haci Murat Gunes, Ibrahim Oguz Karaca, Bilal Boztosun

{"title":"Combined umbilical cord-derived mesenchymal stem cell exosome and cell therapy in heart failure with reduced ejection fraction.","authors":"Berhan Keskin, Beytullah Cakal, Aykun Hakgor, Atakan Dursun, Aysel Akhundova, Umeyir Savur, Ozlem Onder, Haci Murat Gunes, Ibrahim Oguz Karaca, Bilal Boztosun","doi":"10.1080/17460751.2026.2723032","DOIUrl":"10.1080/17460751.2026.2723032","url":null,"abstract":"Background: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) and their exosomes represent potential regenerative approaches for heart failure with reduced ejection fraction (HFrEF). This pilot study evaluated a combined UC-MSC-derived exosome and UC-MSC treatment strategy in HFrEF.Methods: Ten male patients received intracoronary exosomes (20 × 109 particles) followed by two intravenous UC-MSC infusions (1 × 106 cells/kg) at one-week intervals. Echocardiographic parameters, N-terminal pro-B-type natriuretic peptide (NT-proBNP), and 12-item Kansas City Cardiomyopathy Questionnaire (KCCQ-12) scores were assessed longitudinally using linear mixed-effects models.Results: LVEF increased at 3 months (β = 4.45%, p = 0.003), 6 months (β = 5.35%, p < 0.001), and 1 year (β = 4.66%, p = 0.002). Left ventricular dimensions decreased significantly at 6 months and 1 year. Log-transformed NT-proBNP decreased significantly only at 6 months (β = -0.54, p = 0.033), while KCCQ-12 scores did not change significantly. No treatment-related serious adverse events were identified; one patient developed transient pulmonary congestion.Conclusion: The combined strategy was feasible and associated with modest improvements in cardiac function and ventricular dimensions. Given the small, uncontrolled pilot design, these findings are exploratory and do not establish efficacy or safety. Larger randomized controlled studies are required.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"405-412"},"PeriodicalIF":3.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethical, legal, and social issues associated with human fetal tissue research in Japan. 日本与人类胎儿组织研究相关的伦理、法律和社会问题。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-06-01 Epub Date: 2026-06-11 DOI: 10.1080/17460751.2026.2686093

Misao Fujita, Kyoko Akatsuka, Akane Hashimoto, Mari Honda, Tsunakuni Ikka, Minori Kokado, Yoshichika Mori, Masanori Oikawa, Go Okui, Mitinori Saitou, Yasuhiro Takashima

{"title":"Ethical, legal, and social issues associated with human fetal tissue research in Japan.","authors":"Misao Fujita, Kyoko Akatsuka, Akane Hashimoto, Mari Honda, Tsunakuni Ikka, Minori Kokado, Yoshichika Mori, Masanori Oikawa, Go Okui, Mitinori Saitou, Yasuhiro Takashima","doi":"10.1080/17460751.2026.2686093","DOIUrl":"10.1080/17460751.2026.2686093","url":null,"abstract":"The Informed Consent Standards for Human Fetal Tissue Donation for Research, published by the International Society for Stem Cell Research, provide important guidance for ethical research. They are particularly relevant in countries such as Japan, where clear legal frameworks for the use of human fetal and associated tissues obtained through induced abortion are lacking. However, because such guidelines do not fully account for national regulatory and legal frameworks, additional considerations are required to support their effective global implementation. Here, we describe three ethical, legal, and social issues in Japan - also shared in part by other countries - as examples of considerations relevant to the wider implementation of internationally recognized ethical standards. We discuss possible approaches to these issues, including: (1) appropriate selection of medical institutions capable of minimizing the burden on potential donors, (2) consideration of whether consent from the male partner should be obtained, and (3) clarification of the \"Ena (placenta)\" ordinance related to the use and disposal of dead fetuses. Ensuring appropriate integration between such ethical standards and local regulations is essential for the sustainable and ethical development of fetal tissue research.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"425-433"},"PeriodicalIF":3.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148219178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine umbilical cord tissue‑derived MSCs and its conditioned medium promote the healing of excisional wound in rats. 牛脐带组织源性间充质干细胞及其条件培养基促进大鼠切除伤口愈合。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-06-01 Epub Date: 2026-08-18 DOI: 10.1080/17460751.2026.2719471

Kumara Datta Mahesh A, Mousumi Namasudra, Camelia Manna, Saktipada Pradhan, Rabindra Nath Hansda, Sadhan Bag, Samiran Mondal, Kinsuk Das

{"title":"Bovine umbilical cord tissue‑derived MSCs and its conditioned medium promote the healing of excisional wound in rats.","authors":"Kumara Datta Mahesh A, Mousumi Namasudra, Camelia Manna, Saktipada Pradhan, Rabindra Nath Hansda, Sadhan Bag, Samiran Mondal, Kinsuk Das","doi":"10.1080/17460751.2026.2719471","DOIUrl":"10.1080/17460751.2026.2719471","url":null,"abstract":"The goal of skin tissue regeneration is to achieve structural and functional reconstruction, reduce scar formation, and improve wound healing quality. The present study highlights the wound healing and tissue regeneration capability of bovine umbilical cord-derived mesenchymal stem cells (bUC-MSCs) and its cell culture supernatant, i.e. conditioned media (CM). The cells were isolated and characterized from umbilical tissue off-cut. Excisional wound model was created in adult rats. In separate groups the bUC-MSCs and reconstituted lyophilized-CM were injected subcutaneously around the wound periphery at a regular interval for 3 weeks. Significant improvement (p < 0.05) in wound contraction percentage was noticed in bUC-MSC and CM groups as early as day 2. The histo-morphological analysis revealed that in bUC-MSC and CM-treated groups enhanced monocytoid cell migration was noticed in inflammatory phase, exhibited superior collagen organization, and wound remodeling, which are critical for minimizing scar formation and ensuring functional recovery of the skin. The sustained effects observed after four weeks suggest that both bUC-MSCs and CM not only accelerate early healing processes but also contribute to the structural integrity and durability of the healed tissue. Overall, bUC-MSCs and its conditioned media represent a valuable addition to the toolkit of regenerative medicine.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"393-404"},"PeriodicalIF":3.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148797535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Industry updates in advanced therapy medicinal products and regenerative medicine - February 2026. 先进治疗药物产品和再生医学的行业更新- 2026年2月。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-06-01 Epub Date: 2026-03-18 DOI: 10.1080/17460751.2026.2646590

Dusko Ilic, Mirjana Liovic

引用次数: 0

Two-layered electrospun nanofibrous mat loaded chitosan nanoparticles, growth factors and hair follicle bulge stem cells can promote wound healing in rat. 负载壳聚糖纳米粒子、生长因子和毛囊膨出干细胞的两层电纺纳米纤维垫可促进大鼠伤口愈合。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-06-01 Epub Date: 2026-08-04 DOI: 10.1080/17460751.2026.2701682

Asma Moghaddam, Mahmoud Orazizadeh, Vahid Bayati, Fereshteh Nejaddehbashi

{"title":"Two-layered electrospun nanofibrous mat loaded chitosan nanoparticles, growth factors and hair follicle bulge stem cells can promote wound healing in rat.","authors":"Asma Moghaddam, Mahmoud Orazizadeh, Vahid Bayati, Fereshteh Nejaddehbashi","doi":"10.1080/17460751.2026.2701682","DOIUrl":"10.1080/17460751.2026.2701682","url":null,"abstract":"Background and aims: Wound healing remains a demanding medical problem despite significant advances in regenerative medicine. Mesenchymal, hair follicle and pluripotent stem cells are candidate populations for therapeutically relevant applications. Hair follicle bulge stem cells with scaffolds and growth factors can promote the healing of wounds.Methods: This study explores a dual-layer electrospun nanofibrous wound dressing composed of PCL/SSD and PCL/COLL, enhanced with chitosan nanoparticles loaded with EGF and bFGF growth factors. Hair follicle bulge stem cells were seeded onto the scaffold and transplanted into rat skin wounds.Results: FE‑SEM confirmed that the chitosan microspheres were uniformly spherical, ranging from nanoscale to ~1 µm. Thermal analysis showed a 28% weight loss up to 250°C and major degradation between 230°C and 450°C, indicating good thermal stability. About 50% of the encapsulated EGF and bFGF (6.5 ng from 2 mg microspheres) was released within 24 h, followed by sustained release. Biological assays demonstrated strong cell attachment, enhanced collagen deposition, and rapid tissue remodeling, with complete skin regeneration achieved by day 14.Conclusion: These findings suggest that a two-layered electrospun nanofibrous mat incorporating chitosan nanoparticles, growth factors, and hair follicle bulge stem cells significantly enhances wound healing in a rat model.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"413-424"},"PeriodicalIF":3.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148670607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Industry updates in advanced therapy medicinal products and regenerative medicine - April 2026. 先进治疗药物产品和再生医学的行业更新- 2026年4月。

IF 2.6 4区医学

Regenerative medicine Pub Date : 2026-05-25 DOI: 10.1080/17460751.2026.2677892

Dusko Ilic, Mirjana Liovic

引用次数: 0

Extracellular vesicle therapy for spinal cord injury: engineering-enhanced strategies and combination therapies. 脊髓损伤的细胞外囊泡治疗：工程增强策略和联合治疗。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-05-01 Epub Date: 2026-02-24 DOI: 10.1080/17460751.2026.2636634

Lin Zhang, Linlin Dong, Zhongli Dong

引用次数: 0

Establishment and qualification of a GMP-compliant induced pluripotent stem cell master cell bank for clinical applications under the Korean MFDS framework. 在韩国MFDS框架下建立并鉴定符合gmp的诱导多能干细胞主细胞库，用于临床应用。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-05-01 Epub Date: 2026-05-13 DOI: 10.1080/17460751.2026.2671859

Hyo-Won Han, Youngsun Lee, Shin-Jeong Lee, Suyeon Kang, Enna Han, Na Geum Lee, Woondong Cho, Hye Young Choi, Eun Hee Jo, Gueyoun Go, Hyung-Eun Kim, Min Ji Lee, Jee Eun Oh, Yong-Ou Kim, Young-sup Yoon, Ji Yoon Lee

{"title":"Establishment and qualification of a GMP-compliant induced pluripotent stem cell master cell bank for clinical applications under the Korean MFDS framework.","authors":"Hyo-Won Han, Youngsun Lee, Shin-Jeong Lee, Suyeon Kang, Enna Han, Na Geum Lee, Woondong Cho, Hye Young Choi, Eun Hee Jo, Gueyoun Go, Hyung-Eun Kim, Min Ji Lee, Jee Eun Oh, Yong-Ou Kim, Young-sup Yoon, Ji Yoon Lee","doi":"10.1080/17460751.2026.2671859","DOIUrl":"10.1080/17460751.2026.2671859","url":null,"abstract":"Background: Human induced pluripotent stem cells (iPSCs) are a promising starting material that can be differentiated into therapeutic cell products for clinical trials. The production of clinically applicable drug products requires good manufacturing practice (GMP)-compliant iPSCs as the initial starting material. Under the framework of the Korean Ministry of Food and Drug Safety, we generated a master cell bank (MCB) of a human iPSC line, KNIH01-MCB, from peripheral blood mononuclear cells obtained from a healthy female donor under a GMP system.Material and methods: To verify cell identity and quality, we performed a risk assessment of genetic stability, pluripotency, cell line identity, and sterility. For this purpose, we used validated methods, including polymerase chain reaction, karyotyping, short tandem repeat analysis, ABO/human leukocyte antigen (HLA) typing, and bacterial growth testing. After material qualification, the cells underwent comprehensive quality testing, including viral screening, ABO/HLA typing, and sterility testing.Results: We successfully established an MCB of the iPSC line, enabling clinical readiness, including full traceability and donor eligibility for clinical applications. The MCB met the quality control criteria of our in-house GMP system.Conclusion: These iPSCs provide a GMP-compliant and clinically applicable starting material for therapeutic cell manufacturing intended for human use.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"313-323"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13271339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147933007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements. 通过有效支架设计优化肌腱组织工程策略：近期进展综述。

IF 3 4区医学

Regenerative medicine Pub Date : 2026-05-01 Epub Date: 2026-05-18 DOI: 10.1080/17460751.2026.2672913

Gabrielle Lynn Hamner, Justin Lee Brown

{"title":"Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements.","authors":"Gabrielle Lynn Hamner, Justin Lee Brown","doi":"10.1080/17460751.2026.2672913","DOIUrl":"10.1080/17460751.2026.2672913","url":null,"abstract":"Tendon injuries pose a significant global health challenge due to the poor innate healing capacity of the tissue. Current clinical interventions report limited treatment efficacy, typically resulting in a fibrotic scar that is mechanically inferior to native tissue and predisposed to future re-rupture. Tendon tissue engineering, leveraging advances of biomimetic scaffolds, offers a promising path toward functional regeneration rather than fibrotic repair. The design of these biomimetic scaffolds is a complex, interdisciplinary challenge that has evolved from a simple structural replacement to a sophisticated, bio-instructive strategy. Literature searches were conducted using PubMed, Scopus, and R Discovery with the terms \"tendon tissue engineering scaffold\" or \"tendon regenerative medicine scaffold,\" and studies published between 2020 and 2025 were included. In this review, we outline four key design principles identified to enhance tissue engineering solutions constructed for tendon: 1) Biomechanical compatibility, 2) Biocompatibility and Integration, 3) Porosity and Mass Transport, and 4) Mechanobiological Stimuli. Together, these core parameters mediate the production of hierarchical, bio-instructive scaffolds that integrate cellular components, growth factors, and mechanical stimulation to produce intelligent therapeutic systems for functional, long-lasting tendon regeneration.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"337-351"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13271277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147974699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring gene therapy for developmental and epileptic encephalopathies (DEEs): possibilities or promises? 探索发育性和癫痫性脑病的基因治疗：可能性还是希望？

IF 3 4区医学

Regenerative medicine Pub Date : 2026-05-01 Epub Date: 2026-05-28 DOI: 10.1080/17460751.2026.2678016

Anna Behr, Miguel Chillón

引用次数: 0