Experimental and computational approach to establish fit-for-purpose cell viability assays.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING

Regenerative medicine Pub Date : 2024-01-01 Epub Date: 2024-01-22 DOI:10.2217/rme-2023-0154

Laura Pierce, Hidayah Anderson, Swarnavo Sarkar, Steven R Bauer, Sumona Sarkar

引用次数: 0

Abstract

Aim: Cell viability assays are critical for cell-based products. Here, we demonstrate a combined experimental and computational approach to identify fit-for-purpose cell assays that can predict changes in cell proliferation, a critical biological response in cell expansion. Materials & methods: Jurkat cells were systematically injured using heat (45 ± 1°C). Cell viability was measured at 0 h and 24 h after treatment using assays for membrane integrity, metabolic function and apoptosis. Proliferation kinetics for longer term cultures were modeled using the Gompertz distribution to establish predictive models between cell viability results and proliferation. Results & conclusion: We demonstrate an approach for ranking these assays as predictors of cell proliferation and for setting cell viability specifications when a particular proliferation response is required.

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通过实验和计算方法建立适用的细胞活力测定方法。

目的：细胞活力测定对细胞产品至关重要。在此，我们展示了一种实验与计算相结合的方法，以确定可预测细胞增殖（细胞扩增过程中的一种关键生物反应）变化的适用细胞检测方法。材料与方法使用热（45 ± 1°C）对 Jurkat 细胞进行系统损伤。处理后 0 小时和 24 小时，使用膜完整性、代谢功能和细胞凋亡测定法测量细胞活力。使用贡珀兹分布对长期培养的增殖动力学进行建模，以建立细胞存活率结果与增殖之间的预测模型。结果与结论：我们展示了一种方法，可将这些检测结果作为细胞增殖的预测指标进行排序，并在需要特定增殖反应时设定细胞存活率规格。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.