Reactive & Functional Polymers最新文献_第8页

Hot-melt polyurethane adhesive using dynamic reversible phenol−carbamate bonds 热熔聚氨酯胶采用动态可逆苯酚-氨基甲酸酯键

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-12 DOI: 10.1016/j.reactfunctpolym.2025.106337

Ismail Omrani, Mahdi Khoeini, Morteza Behzadnasab

{"title":"Hot-melt polyurethane adhesive using dynamic reversible phenol−carbamate bonds","authors":"Ismail Omrani, Mahdi Khoeini, Morteza Behzadnasab","doi":"10.1016/j.reactfunctpolym.2025.106337","DOIUrl":"10.1016/j.reactfunctpolym.2025.106337","url":null,"abstract":"<div><div>Adhesives are essential in several applications, including transportation, construction, electronics, aircraft, and human organs. However, due to their cross-linked chemical structure, industrial polyurethane hot melt adhesives utilized worldwide are non-recyclable. Here, we developed a facile route to polyurethane hot-melt adhesive (PU-HMA) synthesis by incorporating a phenol-carbamate bond into a three-dimensional dynamically cross-linked polyurethane system, which is notably reworkable. Reversible covalent phenol-carbamate bonds have led to the development of a new class of PU-HMAs. This system combines the advantages of reworkable thermoplastic polyurethane hot-melt adhesives and highly reactive polyurethane hot-melt adhesives, enabling efficient bonding and debonding on demand. Such a structure creates a highly cross-linked covalent network, resulting in reversible adhesive properties that enhance the overall performance of hot-melt adhesives. Two types of PU-HMAs with different crosslinking densities were prepared. As a result, the adhesive exhibited an impressive initial and ultimate adhesion strength for PU-HMA-1 (8.8 ± 1.09 MPa) and PU-HMA-2 (5.45 ± 0.39 MPa), respectively. This adhesive exhibited superior solvent resistance and commendable endurance. In PU-HMA-1, it retained at least 23 % of its initial lap shear strength; in PU-HMA-2, it retained at least 38 %. Remarkably, the advantageous repeated adhesive capability was maintained even after being completely broken three times. After three cycles, PU-HMA-1 experienced a decrease in lap shear strength of only 1 %, while PU-HMA-2, with its high cross-linking density, experienced a significant decrease of 38 %.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106337"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synthesis of fluorescent probes for the metal ion detection 金属离子检测用荧光探针的合成

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-12 DOI: 10.1016/j.reactfunctpolym.2025.106332

Xiujuan Gao , Yichuan Zhang , Mingming Guo

{"title":"The synthesis of fluorescent probes for the metal ion detection","authors":"Xiujuan Gao , Yichuan Zhang , Mingming Guo","doi":"10.1016/j.reactfunctpolym.2025.106332","DOIUrl":"10.1016/j.reactfunctpolym.2025.106332","url":null,"abstract":"<div><div>Fluorescent probes generally consisting of a fluorophore and a recognition part can detect and label various substances through the changes of fluorescence properties before and after recognition. In this work, we synthesized a new monomer using the heck coupling reaction, and the polymeric fluorescent probes were prepared via free radical polymerization with two structurally similar monomers. The effects of molecular structures on the luminescent properties (intensity and lifetime) were explored systematically. Ten metal ions were screened at their optimal excitation wavelengths to figure out their corresponding specific recognition ions through the change of fluorescence color and fluorescence signals, and it was found that the fluorescence probes and the ions showed a good linear relationship within a detective concentration range and could also be calculated by the limit of detection formula to reach a lower detect limit (LOD, 0.0027 μM) than before (0.12 μM). Then the potential biological application of the polymers was investigated, and it suggested that fluorescent probes have potential application in sensor devices and bioimaging.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106332"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a biocompatible, low-cost reinforcement of methacrylated alginate hydrogels using synthetic crosslinking agents 合成交联剂制备生物相容性、低成本的甲基丙烯酸海藻酸盐水凝胶增强剂

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-12 DOI: 10.1016/j.reactfunctpolym.2025.106330

Carolina Gutierrez Cisneros , Hannah Agten , Elien Derveaux , Peter Adriaensens , Veerle Bloemen , Arn Mignon

{"title":"Development of a biocompatible, low-cost reinforcement of methacrylated alginate hydrogels using synthetic crosslinking agents","authors":"Carolina Gutierrez Cisneros , Hannah Agten , Elien Derveaux , Peter Adriaensens , Veerle Bloemen , Arn Mignon","doi":"10.1016/j.reactfunctpolym.2025.106330","DOIUrl":"10.1016/j.reactfunctpolym.2025.106330","url":null,"abstract":"<div><div>The skin is a vital organ that protects the human body, making it highly susceptible to injury. When wounds fail to heal properly, they can become chronic and prone to infection. Hydrogel-based wound dressings, made from natural and synthetic polymers, play a crucial role in wound management by enhancing the wound environment, providing a protective interface over the wound, retaining moisture, and facilitating ease of removal which supports the formation of new tissue. Among these, methacrylated alginate hydrogels show promising biological potential but require improvement in mechanical strength to meet clinical demands due to their large moisture uptake capacity. In this study, methacrylated alginate hydrogels were reinforced with four low-cost (meth)acrylic crosslinkers: ethylene glycol diacrylate, ethylene glycol dimethacrylate, triethylene glycol dimethacrylate and pentaerythritol triacrylate, to improve mechanical properties without compromising biological efficacy. The best formulation, using the first one, demonstrated a swelling capacity of 40 g of water per g of hydrogel, significantly outperforming commercial products like Kaltostat (25 g/g) and AquacelAg (18 g/g). Its mechanical strength (∼0.06 MPa) was comparable to DuoDERM-ET (0.07 MPa) and Mepilex (∼0.05 MPa). The hydrogel also demonstrated excellent in vitro biocompatibility, positioning it as a simple, cost-effective alternative to reinforce methacrylated hydrogels so they eventually become possibly useful as wound dressings, mechanically and physically strongly competing with commercial dressings, while still offering room for future bioactive development to enhance wound healing.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106330"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ polysaccharide-based double-network hydrogels as a sustained kartogenin delivery system for cartilage tissue engineering 基于原位多糖的双网络水凝胶作为软骨组织工程中持续的核原蛋白递送系统

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-11 DOI: 10.1016/j.reactfunctpolym.2025.106335

Yaohui Fan , Junyi Yan , Xinxin Zhao , Wenyuan Wang , Chihao Gao , Xiaoli Lin , Yu Wang , Bo Cheng , Jing Yang , Binbin Li , Xinyu Wang

{"title":"In situ polysaccharide-based double-network hydrogels as a sustained kartogenin delivery system for cartilage tissue engineering","authors":"Yaohui Fan , Junyi Yan , Xinxin Zhao , Wenyuan Wang , Chihao Gao , Xiaoli Lin , Yu Wang , Bo Cheng , Jing Yang , Binbin Li , Xinyu Wang","doi":"10.1016/j.reactfunctpolym.2025.106335","DOIUrl":"10.1016/j.reactfunctpolym.2025.106335","url":null,"abstract":"<div><div>In situ forming biodegradable hydrogel scaffolds are crucial in tissue engineering and drug delivery systems, with extensive applications in articular cartilage (AC) repair. Polysaccharide-based hydrogels are ideal materials for cartilage tissue engineering owning to their biochemical and structural resemblance to the native extracellular matrix (ECM). In this study, a double-network (DN) hydrogel was fabricated through Schiff base reaction and photocrosslinking, utilizing glycidyl methacrylate-modified chitosan (GCS) and aldehyde-modified hyaluronic acid methacrylate (AHM) as precursors. To prepare the drug-loaded hydrogel, we grafted the bioactive factor kartogenin (KGN) onto GCS, generating KGN-conjugated GCS (KGCS), which subsequently formed a crosslinked network with AHM. In contrast to conventional hydrogels encapsulating either drug alone or drug-loaded microspheres, the proposed hydrogel demonstrated a more stable and sustained release profile. Furthermore, the physicochemical properties of both hydrogels were systematically characterized, comprising morphology, mechanical properties and swelling rate. The biocompatibility of hydrogels was comprehensively investigated through in vitro cell proliferation assays, hemocompatibility analysis, and in vivo evaluation of subcutaneous implant. Subsequently, a cartilage defect model was utilized to assess their efficacy in cartilage regeneration. In summary, the two DN hydrogels exhibited suitable porous structure, mechanical properties, and swelling rates. Furthermore, they demonstrated excellent biocompatibility and significantly promoted cartilage defect regeneration.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106335"},"PeriodicalIF":4.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the efficacy of polyethyleneimine cross-linked non-reducing oligosaccharides in gene delivery to cancer cells 聚乙烯亚胺交联非还原性低聚糖在癌细胞基因传递中的作用研究

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-11 DOI: 10.1016/j.reactfunctpolym.2025.106321

Ting Ju , Fangyuan Duan , Jiayu Wang , Yishu Yin , Weihong Lu

{"title":"Investigation of the efficacy of polyethyleneimine cross-linked non-reducing oligosaccharides in gene delivery to cancer cells","authors":"Ting Ju , Fangyuan Duan , Jiayu Wang , Yishu Yin , Weihong Lu","doi":"10.1016/j.reactfunctpolym.2025.106321","DOIUrl":"10.1016/j.reactfunctpolym.2025.106321","url":null,"abstract":"<div><div>For gene delivery, cationic polymers must balance two crucial parameters: transfection efficiency and cytotoxicity. Although PEI remains a widely used gene vector, its clinical translation has been hindered by significant toxicity concerns. In this study, three low-molecular-weight PEI derivatives modified with oligosaccharides, trehalose, raffinose, and stachyose, are referred to as TRON-PEI, RAON-PEI, and STON-PEI, respectively. The potential of these polymers as safe gene delivery systems was explored and the influence of oligosaccharide modifications on the transfection efficiency of PEI-based vectors was examined. The physicochemical properties of the three PEI variants, including their ability to bind pDNA, cytotoxicity, zeta potential, and complex size, were analyzed. The experimental results revealed that oligosaccharide-modified PEI polymers effectively condensed pDNA into nanoparticles with diameters ranging from 106 to 142 nm. Among these variants, RAON-PEI-3 and TRON-PEI-3 exhibited optimal performance, achieving high transfection efficiency with minimal cytotoxicity across multiple cell lines (HeLa, MHCC-97H, and HepG2). Cellular <em>co</em>-localization and molecular docking assays demonstrated that the three vectors could successfully bind to pDNA and that the three polymers were able to target receptor proteins (ASGP-R and galectin-2) on the membrane surface of cancer cells. In addition, the cellular co-localization assay successfully verified that RAON-PEI-3 and TRON-PEI-3 were capable of rapid lysosomal escape and mitochondrial targeting. When complexed with the p53 plasmid, these vectors successfully mediated tumor cell apoptosis, as confirmed by western blotting and live/dead assays. Our results demonstrated that oligosaccharide modification represents a promising strategy for developing PEI-based gene delivery systems with enhanced safety profiles and therapeutic potential.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106321"},"PeriodicalIF":4.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous toughening and strengthening of epoxy-anhydride thermosets using a glycidyl ether of eugenol-grafted polysiloxane without sacrificing thermal performance 用丁香酚接枝聚硅氧烷的缩水甘油酯醚同时增韧和强化环氧酸酐热固性材料而不牺牲热性能

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-11 DOI: 10.1016/j.reactfunctpolym.2025.106333

Zhenzhen Li, Kaili Xie, Ming Huang, Wei Hu, Qiunan Xie, Feiyu Zhu, Jingcheng Liu, Xiaojie Li, Wei Wei

{"title":"Simultaneous toughening and strengthening of epoxy-anhydride thermosets using a glycidyl ether of eugenol-grafted polysiloxane without sacrificing thermal performance","authors":"Zhenzhen Li, Kaili Xie, Ming Huang, Wei Hu, Qiunan Xie, Feiyu Zhu, Jingcheng Liu, Xiaojie Li, Wei Wei","doi":"10.1016/j.reactfunctpolym.2025.106333","DOIUrl":"10.1016/j.reactfunctpolym.2025.106333","url":null,"abstract":"<div><div>The brittleness of cured epoxy resin significantly impacts its reliability as an electronic packaging material. Researchers have consistently aimed to enhance its toughness without compromising the mechanical strength, glass transition temperature (<em>T</em><sub>g</sub>), and thermal stability. In this study, we initially synthesized a polysiloxane containing eugenol structure at the end and side chains through silicon‑hydrogen addition reaction. Subsequently, a glycidyl ether of eugenol-grafted polysiloxane (PMES-EP) was obtained by epoxidation using epichlorohydrin. For modifying the diglycidyl ether of bisphenol A (DGEBA)-based epoxy/anhydride thermoset, PMES-EP participated in the curing reaction, while had little influence on the curing process of the system. PMES-EP exhibited good compatibility with the epoxy system, and it improved the cross-linking density of the cured product, as well as introducing flexible polysiloxane chain segments into the network structure. Therefore, the addition of PMES-EP could effectively toughen and strengthen the epoxy/anhydride thermoset, meanwhile, maintain a high <em>T</em><sub>g</sub> and initial thermal decomposition temperature (<em>T</em><sub>d5%</sub>). Compared with the pure epoxy thermoset, the PMES-EP modified epoxy thermoset with PMES-EP accounting for 20 wt% of DGEBA showed a 21.3 % and 58.8 % increase in tensile strength and impact strength, respectively, a 95.6 % retention in <em>T</em><sub>g</sub>, and no decrease in <em>T</em><sub>d5%</sub>. Furthermore, the water contact angle test also revealed an enhancement of hydrophobicity of the epoxy thermoset by adding PMES-EP, due to the low surface energy of the polysiloxane chain structure. This study provides an effective strategy for toughening and strengthening of epoxy resin in electronic packaging field.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106333"},"PeriodicalIF":4.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal performance and mechanism of Hg(II) by modified chitosan as heavy metal flocculant 改性壳聚糖作为重金属絮凝剂去除Hg（II）的性能及机理

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-11 DOI: 10.1016/j.reactfunctpolym.2025.106334

Lingling Li, Gang Wang, Meng Duan, Kai Yang, Jinling Ma, Kui Huang

{"title":"Removal performance and mechanism of Hg(II) by modified chitosan as heavy metal flocculant","authors":"Lingling Li, Gang Wang, Meng Duan, Kai Yang, Jinling Ma, Kui Huang","doi":"10.1016/j.reactfunctpolym.2025.106334","DOIUrl":"10.1016/j.reactfunctpolym.2025.106334","url":null,"abstract":"<div><div>The macromolecular flocculant, dithiocarboxyl chitosan (DTCTS), was employed to investigate its efficacy in removing Hg(II) from water samples. The optimum hydraulic conditions were determined by orthogonal experiments. Flocculation tests were performed to examine the influences of DTCTS dosage, initial Hg(II) concentration, pH, coexisting turbidity, and coexisting organic substances on the effectiveness of Hg(II) removal. Characterization techniques including fractal dimension, FTIR, SEM, and XPS were utilized to elucidate the removal mechanism of DTCTS for Hg(II). The findings demonstrated a gradual improvement in the Hg(II) removal with increasing DTCTS dosage until reaching a plateau. Within a pH range of 2.0–7.0, DTCTS exhibited remarkable effectiveness in removing Hg(II), achieving a maximum removal efficiency of 99.93 % at pH 6.0, and fractal dimension (<em>D</em><sub>2</sub>) could reach a high value of 1.5281, which accelerated the sedimentation and separation of the flocs. Furthermore, higher initial Hg(II) concentration led to enhanced the Hg(II) removal by DTCTS. Coexisting turbidity and organic substances predominantly exerted inhibitory effects on the removal process; however, when low dosages of DTCTS were used, humic acid showed a promoting effect on Hg(II) removal. The interaction between dithiocarboxyl groups and amine groups in the molecular chain of DTCTS facilitated chelation and coordination with Hg(II), resulting in floc formation mediated by adsorption bridging and net sweeping.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106334"},"PeriodicalIF":4.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and characterization of succinylated pectin hydrogels with enhanced swelling performances 具有增强膨胀性能的琥珀酰化果胶水凝胶的合成与表征

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-08 DOI: 10.1016/j.reactfunctpolym.2025.106331

Stefano Liotino , Stefania Cometa , Stefano Todisco , Piero Mastrorilli , Carlos Bengoechea , Antonio Salomone , Elvira De Giglio

{"title":"Synthesis and characterization of succinylated pectin hydrogels with enhanced swelling performances","authors":"Stefano Liotino , Stefania Cometa , Stefano Todisco , Piero Mastrorilli , Carlos Bengoechea , Antonio Salomone , Elvira De Giglio","doi":"10.1016/j.reactfunctpolym.2025.106331","DOIUrl":"10.1016/j.reactfunctpolym.2025.106331","url":null,"abstract":"<div><div>A novel polymeric material was obtained through succinylation of pectin (S-Pec), resulting in greater stability, film-forming ability, transparency, swelling, and water retention capacity compared to native pectin (Pec). Spectroscopic techniques confirmed the success of the succinylation reaction performed on pectin, employing the reaction of galacturonic acid with succinic anhydride as a model reaction under similar experimental conditions. Moreover, fluorinated succinic anhydride was used to gain insight into the succinylation degree by X-ray Photoelectron Spectroscopy, and a different thermal behavior of S-Pec compared to Pec was confirmed through thermoanalytical characterization. Additionally, the effect of cross-linking either Pec or S-Pec in the presence of divalent cations (i.e., calcium or magnesium ions) on water retention capacity and stability was tested. A significant improvement in the ability to absorb and retain water or saline solution was found for magnesium-crosslinked succinylated pectin, while the in vitro hydrogel stability was higher for the calcium-crosslinked one. The obtained polymer represents a promising substrate for the development of natural-based superabsorbent polymers.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106331"},"PeriodicalIF":4.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of glutaryl thiourea crosslinked novel hydrogel; a detailed analysis of biocompatibility, angiogenesis, and antibacterial potential 戊二酰硫脲交联新型水凝胶的研制与表征详细分析生物相容性、血管生成和抗菌潜力

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-07 DOI: 10.1016/j.reactfunctpolym.2025.106313

Sadia Mehmood , Shanza Khan , Muhammad Haseeb Nawaz , Muhammad Atiq Ur Rehman , Muhammad Arfan , Mudassir Iqbal

{"title":"Development and characterization of glutaryl thiourea crosslinked novel hydrogel; a detailed analysis of biocompatibility, angiogenesis, and antibacterial potential","authors":"Sadia Mehmood , Shanza Khan , Muhammad Haseeb Nawaz , Muhammad Atiq Ur Rehman , Muhammad Arfan , Mudassir Iqbal","doi":"10.1016/j.reactfunctpolym.2025.106313","DOIUrl":"10.1016/j.reactfunctpolym.2025.106313","url":null,"abstract":"<div><div>In this study, we successfully synthesized four novel chitosan hydrogels by carefully crosslinking them with varying concentrations of glutaryl di-isothiocyanate (GTU). This thoughtful approach not only imparts a subtle yellowish tint to the polymer films but also enhances their potential applications as biomaterials. Comprehensive characterization using FTIR, XRD, and CHNS analyses provided important insights into the successful chemical interactions within the hydrogel matrix. Scanning electron microscopy (SEM) revealed a well-defined porous microstructure in the films. Thermal degradation analysis indicated that 50 % weight loss occurred at 270 °C for the crosslinked hydrogel GTU-CS4, compared to 303 °C for pure chitosan, suggesting a manageable decrease in thermal stability due to crosslinking. Mechanical testing showed that increasing the crosslinker concentration by 1.2 mmol resulted in an impressive percentage elongation of the hydrogel films at 30.64 %, in contrast to 18 % for pure chitosan, highlighting significant improvements in flexibility and strength. Our biological evaluations suggested that higher crosslinker concentrations effectively reduced inflammation and promoted angiogenesis, with blood vessel formation increasing to 28 vessels and an enhanced expression of transforming growth factor-beta 1 (TGF-β1). The WST-8 assay demonstrated a noteworthy increase in cell viability, reaching as high as 180 % in the crosslinked hydrogels. Additionally, antibacterial tests showed promising inhibition rates of 80 % against <em>Staphylococcus aureus</em> and 60 % against <em>Escherichia coli</em>. These findings underscore the considerable potential of GTU-crosslinked chitosan hydrogels in advancing skin tissue regeneration applications,</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106313"},"PeriodicalIF":4.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next-generation spectroscopic probe for alpha-amylase detection: Development of a potential biomarker for cancer 用于α -淀粉酶检测的下一代光谱探针：一种潜在的癌症生物标志物的发展

IF 4.5 3区工程技术

Reactive & Functional Polymers Pub Date : 2025-05-07 DOI: 10.1016/j.reactfunctpolym.2025.106320

Julio C. Velazquez-Infante , Carolina Salinas Domján , M. Andrea Molina Torres , Natalia L. Pacioni , Mauro Valente , Marcelo R. Romero , Cesar G. Gomez

{"title":"Next-generation spectroscopic probe for alpha-amylase detection: Development of a potential biomarker for cancer","authors":"Julio C. Velazquez-Infante , Carolina Salinas Domján , M. Andrea Molina Torres , Natalia L. Pacioni , Mauro Valente , Marcelo R. Romero , Cesar G. Gomez","doi":"10.1016/j.reactfunctpolym.2025.106320","DOIUrl":"10.1016/j.reactfunctpolym.2025.106320","url":null,"abstract":"<div><div>A novel spectroscopic probe based on a barbituric acid-starch (ST-BA) derivative is synthesized and used to assess the alpha-amylase activity. This derivative and its precursors are characterized by UV–Visible, fluorescence, FTIR, and <sup>1</sup>H NMR spectroscopy. Luminescent ST-BA core contains a double bond conjugated to pyrimidine ring carbonyl groups, yielding a notable UV–Visible absorption and fluorescence emission. Enzymatic ST-BA cleavage reveals a kinetics slower than for pristine starch, inducing new supramolecular structures with an enhanced fluorescence quantum yield. Force field minimization also supports the contribution of intra and intermolecular interactions to ST-BA oligomer rigidification, a singularity in agreement with aggregation-induced emission. However, the decrease of UV–Visible signal reveals the chromophore competition for the photon after oligomer self-assembly. The linear spectroscopic responses support to ST-BA as a successful enzymatic activity sensor. Although these findings emphasize the ST-BA potential for qualitative enzyme analysis, further experimental condition studies are essential for accurate quantification.</div></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":"214 ","pages":"Article 106320"},"PeriodicalIF":4.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0