Regenerative Therapy最新文献

{"title":"Role of cell-based therapies in digestive disorders: Obstacles and opportunities","authors":"Guodong Liang ,&nbsp;Yuehan Ma ,&nbsp;Ping Deng ,&nbsp;Shufeng Li ,&nbsp;Chunyan He ,&nbsp;Haihang He ,&nbsp;Hairui Liu ,&nbsp;Yunda Fan ,&nbsp;Ze Li","doi":"10.1016/j.reth.2025.02.009","DOIUrl":"10.1016/j.reth.2025.02.009","url":null,"abstract":"<div><div>Stem cell-based therapies have emerged as a promising frontier in the treatment of gastrointestinal disorders, offering potential solutions for challenges posed by conventional treatments. This review comprehensively examines recent advancements in cell-based therapeutic strategies, particularly focusing on stem cell applications, immunotherapy, and cellular therapies for digestive diseases. It highlights the successful differentiation of enteric neural progenitors from pluripotent stem cells and their application in animal models, such as Hirschsprung disease. Furthermore, the review evaluates clinical trials and experimental studies demonstrating the potential of stem cells in regenerating damaged tissues, modulating immune responses, and promoting healing in conditions like Crohn's disease and liver failure. By addressing challenges, such as scalability, immunogenicity, and ethical considerations, the review underscores the translational opportunities and obstacles in realizing the clinical potential of these therapies. Concluding with an emphasis on future directions, the study provides insights into optimizing therapeutic efficacy and fostering innovations in personalized medicine for digestive disorders.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 1-18"},"PeriodicalIF":3.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet concentrates in diabetic foot ulcers: A comparative review of PRP, PRF, and CGF with case insights","authors":"Mengling Yang ,&nbsp;Bo Deng ,&nbsp;Wei Hao ,&nbsp;Xiaoyan Jiang ,&nbsp;Yan Chen ,&nbsp;Min Wang ,&nbsp;Yi Yuan ,&nbsp;Meirong Chen ,&nbsp;Xiaohua Wu ,&nbsp;Chenzhen Du ,&nbsp;David G. Armstrong ,&nbsp;Lian Guo ,&nbsp;Wuquan Deng ,&nbsp;Hongyan Wang","doi":"10.1016/j.reth.2025.02.005","DOIUrl":"10.1016/j.reth.2025.02.005","url":null,"abstract":"<div><div>Diabetic foot ulcers (DFUs) are severe complications of diabetes, often leading to chronic wounds, amputations, and increased mortality risk. Platelet concentrates (PCs)—natural biomaterials utilized in regenerative medicine—have garnered attention for their capacity to enhance tissue repair and wound healing. This study reviews the preparation methods, biological mechanisms, and clinical efficacy of three generations of PCs: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factors (CGF). Comparative analysis reveals that PRP, the first generation, provides abundant growth factors but relies on anticoagulants, which may hinder fibrin formation and tissue adhesion. PRF, as the second generation, eliminates anticoagulants, forming a fibrin matrix that sustains growth factor release and enhances cell migration. CGF, the latest advancement, employs refined centrifugation to achieve higher growth factor concentrations and a denser fibrin matrix, accelerating tissue regeneration. Case series results demonstrated superior wound healing outcomes with CGF, including faster epithelialization and reduced healing time compared to PRP and PRF. These findings underscore CGF's potential as the most effective PC for managing DFUs, supporting its broader clinical adoption in advanced wound care.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 625-632"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the commercial manufacturing of tisagenlecleucel for patients in Japan: A 4-year experiential journey","authors":"Fumiko Iwamoto ,&nbsp;David Fong ,&nbsp;Margarida Rodrigues ,&nbsp;Yoshie Tsurumaki","doi":"10.1016/j.reth.2025.02.001","DOIUrl":"10.1016/j.reth.2025.02.001","url":null,"abstract":"<div><h3>Background</h3><div>Tisagenlecleucel, an autologous CD19-directed chimeric antigen receptor T-cell therapy, was approved in Japan, in March 2019 for patients with relapsed/refractory (r/r) B-cell acute lymphoblastic leukamia and r/r diffuse large B-cell lymphoma, and in August 2022 for patients with r/r follicular lymphoma. Post-approval, a key goal has been to upscale and continuously improve manufacturing and shipment success rates (MSR and SSR, respectively) in the commercial setting to meet the needs of patients worldwide, including in Japan. Herein, we report accrued experience from a 4-year journey of commercial tisagenlecleucel manufacturing process optimization for patients in Japan.</div></div><div><h3>Methods</h3><div>Data were collected from the Novartis tisagenlecleucel manufacturing database, and only commercial tisagenlecleucel orders between August 2019 and July 2023 were included. The data for cancellations after shipment were based on cancellation forms obtained from institutions.</div></div><div><h3>Results</h3><div>Since the first approval of tisagenlecleucel in Japan, its MSR and SSR improved over 4 years. Particularly, the MSR increased from 85.6 % in the first year to 95.3 % in the year 4, while the SSR increased from 92.4 % to 98.4 % in the same period. The corresponding termination and out-of-specification (OOS) rates decreased from 6.8 % to 0.9 %, and from 7.6 % to 3.7 %, respectively. The MSR (87.9 % versus 94.6 %), SSR (93.0 % versus 97.5 %), termination (6.0 % versus 1.9 %), overall OOS rates (6.1 % versus 3.5 %), and viability OOS (2.8 % versus 0.2 %) have improved significantly since the implementation of manufacturing process improvements in 2022.</div></div><div><h3>Conclusion</h3><div>Continuous improvements in the tisagenlecleucel manufacturing process over the years resulted in higher manufacturing success and increased product availability for Japanese patients.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 619-624"},"PeriodicalIF":3.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive and degradable collagen-based three-dimensional scaffold encapsulated with adipose mesenchymal stem cells-derived exosomes improved diabetic wound healing","authors":"Fereshteh Talebpour Amiri ,&nbsp;Melody Omraninava ,&nbsp;Shadman Shahzamani ,&nbsp;Amirali Khodashenas ,&nbsp;Arman Daryakar ,&nbsp;Davood Nasiry","doi":"10.1016/j.reth.2025.02.002","DOIUrl":"10.1016/j.reth.2025.02.002","url":null,"abstract":"<div><div>A diabetic wound is the most frequent form of chronic wound. Because diabetic wounds have multiple factors contributing to their development, the best treatments involve using a combination of approaches. Herein we assessed whether bioactive and degradable bioengineered micro-porous collagen-based three-dimensional scaffold (CTS) encapsulated with adipose mesenchymal stem cells (ASCs)-derived exosomes could accelerate the wound healing process in diabetic rats. Diabetic animals were assigned to the control group, CTS group, Exo group, and CTS+Exo group. Tissue samples were collected on days 7, 14, and 21 for evaluations including stereological, molecular, and tensiometrical assessments. The findings showed that in the treatment groups, there was a notably increase in wound closure rate, total volumes of newly formed epidermis and dermis, numerical densities of fibroblasts and blood vessels, collagen density, and biomechanical parameters than the control group, with the most noticeable changes seen in the CTS+Exo group. Additionally, there was a notably increase in the transcript of miRNA-146a, TGF-β, bFGF, and VEGF genes in the treatment groups than the control group, with the highest expression observed in the CTS+Exo group. In the CTS+Exo group, there was a much greater decrease in TNF-α and IL-1β expression, as well as in the number of neutrophils, compared to the other groups. These results validated that the combination of CTS and ASCs-derived exosomes has a greater effect on improving diabetic wound healing.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 606-618"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electro-spun piezoelectric PLLA smart composites as a scaffold on bone fracture: A review","authors":"Melika Mirghaffari ,&nbsp;Asal Mahmoodiyan ,&nbsp;Shahram Mahboubizadeh ,&nbsp;Atefeh Shahbazi ,&nbsp;Yaser Soleimani ,&nbsp;Sana Mirghaffari ,&nbsp;Zahra Shahravi","doi":"10.1016/j.reth.2025.01.026","DOIUrl":"10.1016/j.reth.2025.01.026","url":null,"abstract":"<div><div>The intrinsic ability of these scaffold materials to generate piezoelectric currents presents a major proposition for increasing the complexity of the scaffold and facilitating tissue healing. The piezoelectric behavior of bone tissue has been an area of interest for many researchers in the past. However, these properties have not been given much attention in new osteochondral tissue scaffold designs, whereas the established design factors mainly concentrate on the structural and mechanical characteristics of the tissue in question. The main advantages of the piezoelectric electrospun scaffolds in tissue engineering consist in the possibility to reproduce the piezoelectric properties of the fibrous extracellular matrix (ECM) of the tissue and the application of combined electrical and mechanic stimulation in the process of bone tissue regeneration. Poly-<span>l</span>-lactic acid (PLLA) has proved to be a potential biomaterial because of its adjustable mechanical characteristics and the bio-degradable capability for the creation of porous scaffolds with micro/nanostructure designs in various techniques. PLLA-based scaffolds can be altered on their surface or can be incorporated with other polymers either natural or synthetic or bioceramic materials. These modifications and combinations are to introduce improvement or changes in the scaffolds for the improvement of the functional properties that favor bone tissue engineering. In this review, we discussed the properties of PLLA and more particularly, the fracture-repairing activity in bone fracture therapy. It also investigates the interaction of PLLA with other biopolymers or biomaterials to dramatically enhance bone scaffolds' performance.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 591-605"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc sulfate gel reshapes the wound microenvironment to promote full-thickness wound healing in mice","authors":"Ying Zhang ,&nbsp;Xuebo Wei ,&nbsp;Yun Xu ,&nbsp;Weidong Xia ,&nbsp;ChaoYu Zheng ,&nbsp;Hongyu Zhang ,&nbsp;Weiting Chen ,&nbsp;Ke Xu ,&nbsp;Qun Huang","doi":"10.1016/j.reth.2025.01.022","DOIUrl":"10.1016/j.reth.2025.01.022","url":null,"abstract":"<div><div>Delayed healing of skin wounds significantly impacts human life, Zinc plays a pivotal role in human growth, the immune system, and various cellular processes. Previous studies have demonstrated that zinc supplementation or topical zinc ion therapy can accelerate wound healing in cases of skin injury. Zinc deficiency has been linked to delayed wound healing, but the role it plays remains to be elucidated. In this study, we report the preparation of zinc sulfate gel to promote wound healing by treating inflammation, antioxidant effects, and angiogenesis. We demonstrated the efficacy of zinc sulfate gel in wound healing in a mouse model of full-thickness skin excision, spared the anti-inflammatory and antioxidant capabilities of zinc ions. Furthermore, Zinc sulfate gel can stimulate tissue to enter the proliferative phase by regulating macrophage polarization, thereby accelerating collagen deposition, granulation tissue formation, and extracellular matrix production and remodeling. In conclusion, zinc ion gel provides a promising strategy for skin wounds regeneration.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 582-590"},"PeriodicalIF":3.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research status of biomaterials based on physical signals for bone injury repair","authors":"Qi Sun ,&nbsp;Chao-Hua Li ,&nbsp;Qi-Shun Liu ,&nbsp;Yuan-Bin Zhang ,&nbsp;Bai-Song Hu ,&nbsp;Qi Feng ,&nbsp;Yong Lang","doi":"10.1016/j.reth.2025.01.025","DOIUrl":"10.1016/j.reth.2025.01.025","url":null,"abstract":"<div><div>Bone defects repair continues to be a significant challenge facing the world. Biological scaffolds, bioactive molecules, and cells are the three major elements of bone tissue engineering, which have been widely used in bone regeneration therapy, especially with the rise of bioactive molecules in recent years. According to their physical properties, they can be divided into force, magnetic field (MF), electric field (EF), ultrasonic wave, light, heat, etc. However, the transmission of bioactive molecules has obvious shortcomings that hinder the development of the tissue-rearing process. This paper reviews the mechanism of physical signal induction in bone tissue engineering in recent years. It summarizes the application strategies of physical signal in bone tissue engineering, including biomaterial designs, physical signal loading strategies and related pathways. Finally, the ongoing challenges and prospects for the future are discussed.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 544-557"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovation in ovary decellularization methods: Chemical and herbal detergents","authors":"Mozafar Khazaei ,&nbsp;Sepideh Kadivarian ,&nbsp;Diyar Qadir Nabi ,&nbsp;Leila Rezakhani","doi":"10.1016/j.reth.2025.01.023","DOIUrl":"10.1016/j.reth.2025.01.023","url":null,"abstract":"<div><div>The ovary provides an ideal environment for egg survival due to its distinct structure that directly contributes to the growth and maintenance of the follicle. The purpose of this study is to compare ovarian decellularization with herbal and chemical detergents. Sheep ovarian was used in this study. 1 % sodium dodecyl sulfate (SDS) as a chemical detergent and 1, 2.5, and 5 % Acanthophyllum (ACP) were used as herbal agents for decellularization. DNA content, histological characteristics, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), biocompatibility, antibacterial test, hemocompatibility, and scanning electron microscope (SEM) were investigated. The results showed the DNA content in decellularization scaffolds with 1 % SDS and 5 % ACP was reduced suitably. Also, histological observations confirmed this finding, and the nuclei were completely removed in these two groups. Disorganization of collagen fibers and tissue architecture was observed more in the SDS group than in the ACP group. No group reported cytotoxicity and the best blood compatibility in decellularization with herbal agents was reported. Protein bands are largely conserved in all methods. Higher antibacterial properties were observed in the decellularization technique with ACP. Decellularization with 5%ACP, in addition to being able to completely remove cells in the tissue, can help preserve the ultrastructure of the ovary. Therefore, this plant agent can be introduced as a decellularization method for studies in this field.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 573-581"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells","authors":"Ashok Kumar Balaraman ,&nbsp;M. Arockia Babu ,&nbsp;Muhammad Afzal ,&nbsp;Gaurav Sanghvi ,&nbsp;Rekha M M ,&nbsp;Sofia Gupta ,&nbsp;Mohit Rana ,&nbsp;Haider Ali ,&nbsp;Kavita Goyal ,&nbsp;Vetriselvan Subramaniyan ,&nbsp;Ling Shing Wong ,&nbsp;Vinoth Kumarasamy","doi":"10.1016/j.reth.2025.01.019","DOIUrl":"10.1016/j.reth.2025.01.019","url":null,"abstract":"<div><div>Recently, increasing interest has been in utilizing mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), especially exosomes, as nanocarriers for miRNA delivery in cancer treatment. Due to such characteristics, nanocarriers are specific: biocompatible, low immunogenicity, and capable of spontaneous tumor accumulation. MSC-EVs were loaded with therapeutic miRNAs and minimized their susceptibility to degradation by protecting the miRNA from accessibility to degrading enzymes and providing targeted delivery of the miRNAs to the tumor cells to modulate oncogenic pathways. In vitro and in vivo experiments suggest that MSC-EVs loaded with miRNAs may inhibit tumor growth, prevent metastasis, and increase the effectiveness of chemotherapy and radiotherapy. However, these improvements present difficulties such as isolation, scalability, and stability of delivered miRNA during storage.</div><div>Furthermore, the issues related to off-target effects, as well as immunogenicity, can be a focus. The mechanisms of miRNA loading into MSC-EVs, as well as their targeting efficiency and therapeutic potential, can be outlined in this manuscript. For the final part of the manuscript, the current advances in MSC-EV engineering and potential strategies for clinical application have been described. The findings of MSC-EVs imply that they present MSC-EVs as a second-generation tool for precise oncology.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 558-572"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcutaneous implantation of tooth germ stem cells over the masseter muscle in mice: An in vivo pilot study","authors":"Nosrat Nourbakhsh ,&nbsp;Ghazaleh Baniebrahimi ,&nbsp;Sahar Talebi ,&nbsp;Ardeshir Talebi ,&nbsp;Mohammad Hossein Nasr Esfahani ,&nbsp;Bijan Movahedian ,&nbsp;Maziar Manshayi ,&nbsp;Navid Naghdi ,&nbsp;Fatemeh Ejeian ,&nbsp;Elahe Masaeli ,&nbsp;Seyed Ali Mosaddad","doi":"10.1016/j.reth.2025.01.021","DOIUrl":"10.1016/j.reth.2025.01.021","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to explore the potential of tooth germ stem cells for regenerating tooth-like structures by subcutaneously implanting first molar tooth germ stem cells over the masseter muscle in mice.</div></div><div><h3>Methods</h3><div>Five pairs of house mice, <em>Mus musculus</em>, were selected for mating. At gestational day 14 (E14), the fetuses were extracted, and the first molar tooth germ at the cap stage was isolated. Tooth germ stem cells were prepared into a suspension and seeded onto scaffolds, which were then implanted subcutaneously over the masseter muscle in male mice. The control group (n = 5 male mice) received acellular scaffolds implanted at the same site. After 20 days, the regenerated tissues were resected and analyzed histologically using hematoxylin and eosin (H &amp; E) staining, Masson's trichrome staining, and immunohistochemical (IHC) staining for cytokeratin (CK) and vimentin markers.</div></div><div><h3>Results</h3><div>H &amp; E staining showed the formation of integrated oval structures at the implant site in all samples. Masson's trichrome staining identified dispersed accumulations of cellular mineralized matrix within the connective tissue. IHC staining was positive for vimentin, confirming the mesenchymal origin of the loose tissue at the center, indicating future dental pulp development. Positive CK staining indicated the ectodermal origin of dense peripheral tissues, suggesting the future formation of inner enamel epithelium. The combined immunohistochemical results for vimentin and CK confirmed the ecto-mesenchymal origin of the regenerated tissue, which resembled a late bell-stage tooth germ observed around gestational days 17.5–18 and showed early indications of dentin formation (D0).</div></div><div><h3>Conclusion</h3><div>The study indicates that tooth germ stem cells may have the potential to produce dense, tooth-like structures when implanted subcutaneously in mice. These findings provide preliminary insights into the possible applications of tooth germ stem cells in regenerative dental tissue engineering.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 536-543"},"PeriodicalIF":3.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}