Regenerative Therapy最新文献

{"title":"Establishment of reproducible method for production of cultured epidermal sheets with controlled melanocyte density","authors":"Mai Mori ,&nbsp;Chie Kobori ,&nbsp;Ryo Takagi ,&nbsp;Moeka Tani ,&nbsp;Hidenori Akutsu ,&nbsp;Akihiro Umezawa","doi":"10.1016/j.reth.2025.04.001","DOIUrl":"10.1016/j.reth.2025.04.001","url":null,"abstract":"<div><h3>Introduction</h3><div>Vitiligo, the most common human pigmentary disorder, significantly impacts patients' quality of life through its profound psychological and social consequences. The condition's complex pathogenesis and substantial effect on patients' well-being underscore the critical need for advanced therapeutic approaches. Treatment for vitiligo by autologous cultured epidermal transplantation has been developed, and the efficacy of this treatment has been determined. However, there is concern that differences in postoperative pigmentation of affected areas may occur due to difficulty in adjusting melanocyte content. This study aimed to develop a reproducible method for fabricating cultured epidermal sheets containing melanocytes for use in clinical applications.</div></div><div><h3>Methods</h3><div>Human epidermal keratinocytes and melanocytes derived from skin tissues of volunteer donors were prepared under different culture conditions and the proliferation of keratinocytes and melanocytes was determined. The density and percentage of melanocytes in cultured epidermal sheets were evaluated by DOPA staining and flow cytometry. Moreover, a novel clinical-grade melanocyte culture medium with specific supplements was developed that avoids bovine pituitary extract containing unknown animal-derived unknown materials.</div></div><div><h3>Results</h3><div>The density of melanocytes was higher when melanocytes were seeded first, as opposed to the simultaneous seeding of melanocytes and keratinocytes. The best condition in the present study for preparing cultured epidermal sheets with high melanocyte density was to seed melanocytes at 1.0 × 10⁴ cells/cm² on Day 1, followed by feeder cells on Day 2 and keratinocytes at 8.0 × 10⁴ cells/cm² on Day 3. The optimal culture medium for melanocytes was determined to contain 10 nM aMSH, 10 nM endothelin-1, and 10 μg/mL FGF-2 as culture supplements.</div></div><div><h3>Conclusions</h3><div>The results of this study establish a foundation for the development of cell therapy effective for vitiligo.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 499-505"},"PeriodicalIF":3.4,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xenograft of bio-3D printed scaffold-free cartilage constructs derived from human iPSCs to regenerate articular cartilage in immunodeficient pigs","authors":"Toshihiro Nonaka ,&nbsp;Anna Nakamura ,&nbsp;Daiki Murata ,&nbsp;Hiromu Yoshizato ,&nbsp;Shohei Kashimoto ,&nbsp;Yukiko Nagaishi ,&nbsp;Manabu Itoh ,&nbsp;Denise Zujur ,&nbsp;Chengzhu Zhao ,&nbsp;Yoshiko Inada ,&nbsp;Makoto Ikeya ,&nbsp;Junya Toguchida ,&nbsp;Masaaki Mawatari ,&nbsp;Koichi Nakayama","doi":"10.1016/j.reth.2025.04.018","DOIUrl":"10.1016/j.reth.2025.04.018","url":null,"abstract":"<div><div>Arthroplasty is currently the only option for reconstruction of large articular cartilage defects, mainly due to osteoarthritis. However, reconstruction with artificial materials has several issues such as deterioration, foreign body reaction, and bacterial infection. This study established a new method for articular cartilage reconstruction that fundamentally solves the problems associated with artificial materials by creating scaffold-free cartilage constructs and implanting them into extensive osteochondral defects without artificial materials. Tubular cartilage constructs were fabricated using a completely scaffold-free Kenzan bio-three-dimensional printing method with chondrogenic spheroids generated from human induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iPSC-MSCs). The constructs were partially cut open to form a patch and implanted into osteochondral defects in the femoral trochlear groove of immunodeficient miniature pigs. The cartilage constructs were elastic and easy to handle, and abundant glycosaminoglycans and collagens were observed in the grafted site after implantation, as well as in the articular cartilage. Cells at this site were positive for human vimentin, indicating that the cartilage constructs were successfully engrafted into the host subchondral bone. Scaffold-free human iPSC-MSC-derived cartilage constructs implanted into osteochondral defects contribute to the regeneration of extensive osteochondral defects in the absence of artificial materials.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 506-516"},"PeriodicalIF":3.4,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the optimal retention method in vivo for stem cell therapy: Low-intensity ultrasound preconditioning","authors":"Haopeng Xu ,&nbsp;Yilin Tang ,&nbsp;Wenjing Mao,&nbsp;Liu Wu,&nbsp;Yiqing Zhou,&nbsp;Juan Deng,&nbsp;Wentao Tang,&nbsp;Xinfang Xiao,&nbsp;Yi Xia,&nbsp;Yan Wang","doi":"10.1016/j.reth.2025.04.012","DOIUrl":"10.1016/j.reth.2025.04.012","url":null,"abstract":"<div><div>Bone marrow mesenchymal stem cells (BMSCs) are pluripotent and self-renewing, exerting a crucial role in the domain of regenerative medicine. Nevertheless, BMSCs encounter challenges such as low cell viability and inadequate homing during transplantation, thereby restricting their therapeutic efficacy. Hence, current research is concentrated on identifying optimal retention approaches following BMSCs transplantation to enhance its effectiveness. Low-intensity ultrasound (LIUS) has been verified as an efficacious method to enhance the performance of BMSCs. We established a skin trauma model and assessed the therapeutic effect of LIUS-preconditioned BMSCs. The results demonstrated that pretreatment with LIUS could expedite wound healing and effectively diminish scar formation post-transplantation by promoting proliferation capacity, reinforcing anti-apoptotic attributes, improving homing ability, and significantly enhancing the transplantation effect of BMSCs. These discoveries imply that LIUS might constitute a promising strategy for attaining optimal retention after stem cell transplantation in regenerative medicine and wound repair therapy.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 484-492"},"PeriodicalIF":3.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research status of regenerative difficulties after central nervous system injury","authors":"Yunxia Fu ,&nbsp;Xi Rui ,&nbsp;Shumin Zhu ,&nbsp;Chenqu Guo ,&nbsp;Haoyang Li ,&nbsp;Zhenhao Pan ,&nbsp;Xuanhao Wu ,&nbsp;Wenpeng He","doi":"10.1016/j.reth.2025.04.011","DOIUrl":"10.1016/j.reth.2025.04.011","url":null,"abstract":"<div><div>Multiple studies have shown that permanent functional disabilities caused after nerve damage are mainly due to the limited ability of damaged neurons in the central nervous system (CNS) to regenerate axons and re-establish functional connections. Most axons in the CNS of adult mammals cannot reactivate their intrinsic growth program after injury, making axonal regeneration difficult when damaged. This article provides a systematic review of the response processes following CNS injury and the factors affecting repair and regeneration, focusing on the molecular mechanisms that regulate the regeneration of damaged axons, in hopes of offering new insights for the repair of CNS injuries.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 493-498"},"PeriodicalIF":3.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo study of concentrated growth factor (CGF) mediating macrophage polarization in bone defect repair","authors":"Deng xin ,&nbsp;Zheng Junwen ,&nbsp;Zou Jiacheng ,&nbsp;Xun Qiongyu ,&nbsp;He Huanzong ,&nbsp;Li peng ,&nbsp;Liao juan","doi":"10.1016/j.reth.2025.04.013","DOIUrl":"10.1016/j.reth.2025.04.013","url":null,"abstract":"<div><div>Concentrated growth factor (CGF) is widely applied in clinical practice, but whether it has bone promoting effects and its mechanism of action are still the focus of discussion. In this study, in vitro experiments demonstrate that CGF can promote the expression of Arg-1 in BMDM cells, facilitating their polarization towards the M2 macrophages and encouraging the secretion of IL-10 and VEGF-A. CGF modulates M1 macrophages by reducing the expression of iNOS, while enhancing Arg-1 expression, thereby converting them to M2 macrophages. This is accompanied by a decrease in the secretion of TNF-<span><math><mrow><mi>α</mi></mrow></math></span> and IL-1β, and an increase in the secretion of IL-10 and VEGF-A. Mechanistically, CGF promotes the phosphorylation of STAT3, which in turn induces M2 macrophage polarization, suggesting that the function of CGF-mediated macrophages may be associated with the STAT3 signaling pathway. Moreover, CGF-mediated macrophages were found to enhance osteoblast activity, increasing the expression of ALP, RUNX2, and BMP-2, and improving cell migration capabilities. In vivo experiments showed that CGF could early recruit M2 macrophages to the bone defect site, promoting the expression of bone formation-related proteins such as ALP and BMP-2, and accelerating bone tissue regeneration. In summary, our study demonstrates that CGF can induce bone repair and regeneration by promoting immune modulation and macrophage polarization.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 474-483"},"PeriodicalIF":3.4,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method standardization of secretome production, collection, and characterization: New insights and challenges","authors":"Nivisti Singh,&nbsp;Yahya E. Choonara,&nbsp;Pradeep Kumar","doi":"10.1016/j.reth.2025.04.005","DOIUrl":"10.1016/j.reth.2025.04.005","url":null,"abstract":"<div><div>Stem cell transplantation remains at the centre of regenerative therapy. Recent, studies have demonstrated that the therapeutic effect of stem cells originates from paracrine reactions from cellular products (secretome) rather than direct cell differentiation. The secretome (cell-free therapy) has the potential to eliminate the various challenges stem cell therapy encounters including apoptotic, inflammation, and non-immunogenic effects. Cell-free therapy is a new emerging field with the number of clinical trials slowly increasing, however, vast research currently is required in this field regarding standardization of protocols used to obtain the secretome. The recent progression of research in secretome production and characterization has created new perceptions of cellular communication and protein dynamics. Standard methods of secretome production, reproducibility, optimization, and bulk production remain a major challenge for scientists. Precise identification of biomarkers and therapeutic targets, through bioinformatics, mass spectrometry, and cell-based systems are promising techniques addressing these limitations. Therefore, this review aims to report on the methods for secretome production, collection, and characterization. Breakthroughs in this field of research will open the doors for personalized medicine, regenerative therapies, and other biotechnological applications.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 466-473"},"PeriodicalIF":3.4,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-145-enriched BMSCs-derived exosomes ameliorate neurogenic erectile dysfunction in aged rats via TGFBR2 inhibition","authors":"Yude Hong ,&nbsp;Zejia Feng ,&nbsp;Yunlong Ge ,&nbsp;Yuhang Xi ,&nbsp;Bowen Zhang ,&nbsp;Jianjie Wu ,&nbsp;Tian Xia ,&nbsp;Bowen Tang ,&nbsp;Wei Wang ,&nbsp;Jun Chen ,&nbsp;Hua Wang ,&nbsp;Hengjun Xiao","doi":"10.1016/j.reth.2025.04.004","DOIUrl":"10.1016/j.reth.2025.04.004","url":null,"abstract":"<div><h3>Background</h3><div>Neurogenic erectile dysfunction (ED) is a prevalent complication following radical prostatectomy in elderly patients, primarily resulting from the apoptosis of corpus cavernosum smooth muscle cells (CCSMCs) and the subsequent excessive fibrosis of the corpus cavernosum.</div></div><div><h3>Aim</h3><div>This study aimed to compare the therapeutic effects of exosomes derived from lentivirus-transfected miR-145 bone marrow mesenchymal stem cells (Exo-145) and unmodified BMSCs-derived exosomes (Exo) in aged rats with bilateral cavernous nerve injury (BCNI) and investigate the underlying mechanisms.</div></div><div><h3>Methods</h3><div>Twenty-four-month-old male rats were assigned to four groups, namely Sham, BCNI, Exo, and Exo-145. Three weeks after treatment, erectile function was assessed by measuring the maximal intracavernosal pressure to mean arterial pressure (ICP/MAP) ratio. Apoptosis and fibrosis were semi-quantitatively analyzed using TUNEL and Masson's trichrome staining, respectively. In vitro, CCSMCs were subjected to H₂O₂-induced oxidative stress, and the protective effects of Exo-145 were evaluated through flow cytometry and Western blot. Lastly, the targets and mechanisms of miR-145 were further validated using dual-luciferase reporter assays and rescue experiments.</div></div><div><h3>Results</h3><div>Exo-145 significantly outperformed Exo in restoring erectile function in aged BCNI rats, as evidenced by the significantly higher maximal ICP/MAP ratio, a marked reduction in TUNEL-positive cell count, and marked suppression of fibrosis in cavernous tissue. Moreover, Masson's trichrome staining displayed a substantial decrease in collagen deposition. In vitro, Exo-145 alleviated H₂O₂-induced apoptosis in CCSMCs by downregulating Cleaved Caspase-3 expression and Bax while concurrently upregulating Bcl-2 expression. TGFBR2 was identified as a direct target of miR-145 through dual-luciferase reporter assays, with its overexpression partially reversing the protective effects of Exo-145.</div></div><div><h3>Conclusion</h3><div>Exo-145 demonstrates superior efficacy compared to Exo in treating aged neurogenic ED by targeting TGFBR2 to alleviate apoptosis and fibrosis. It may represent a promising cell-free therapeutic option for neurogenic erectile dysfunction in elderly patients and could offer new perspectives for improving their prognosis.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 455-465"},"PeriodicalIF":3.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lineage plasticity and reprogramming of epithelial cells during tissue injury and regeneration-lessons from the lineage plasticity of hepatocytes and cholangiocytes induced by liver injury–","authors":"Kenji Aoshima ,&nbsp;Naoki Tanimizu","doi":"10.1016/j.reth.2025.04.008","DOIUrl":"10.1016/j.reth.2025.04.008","url":null,"abstract":"<div><div>Aside from successfully generating induced pluripotent stem cells from somatic cells, “reprogramming of differentiated cells” is a major technique for obtaining any type of target cells in vitro and in vivo by introducing critical transcription factors and using chemical compound combinations. In addition to these technical aspects, the physiological relevance of reprogramming has been extensively investigated, as growing evidence suggests that cellular reprogramming resulting in dedifferentiation or lineage conversion of a specific type of cell is associated with tissue injury and regeneration. We focus on cellular reprogramming in acute and chronically injured livers and summarize the molecular mechanisms governing the lineage transition between two types of liver epithelial cells, hepatocytes, and cholangiocytes.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 447-454"},"PeriodicalIF":3.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma therapy for knee osteoarthritis: Insights from real-world clinical data in Japan","authors":"Yoshitomo Saita ,&nbsp;Yohei Kobayashi ,&nbsp;Sayuri Uchino ,&nbsp;Hirofumi Nishio ,&nbsp;Takanori Wakayama ,&nbsp;Shin Fukusato ,&nbsp;Yasumasa Momoi ,&nbsp;Ryosuke Nakajima ,&nbsp;Nanako Yamamoto ,&nbsp;Shinnosuke Hada ,&nbsp;Haruka Kaneko ,&nbsp;Muneaki Ishijima","doi":"10.1016/j.reth.2025.04.002","DOIUrl":"10.1016/j.reth.2025.04.002","url":null,"abstract":"<div><h3>Introduction</h3><div>Knee osteoarthritis (KOA) is highly prevalent, and platelet-rich plasma (PRP) therapy has emerged as a novel treatment option. Although PRP is promising, its effectiveness remains controversial. In Japan, PRP therapy is administered as a self-funded treatment in specialized clinics. The National Regenerative Medicine Database (NRMD) was established to support the evaluation of regenerative therapies. This study aimed to analyze patient characteristics and treatment outcomes of PRP therapy for KOA in our hospital registered in NRMD database and to identify factors that influence therapeutic effectiveness.</div></div><div><h3>Methods</h3><div>This retrospective observational study analyzed PRP treatment data for KOA in our hospital from January 2020 to May 2024 which was registered in the NRMD. Leukocyte-poor PRP (LP-PRP) was prepared using the MyCells kit, and patients received three injections at 3- to 5-week intervals. The eligibility criteria included chronic knee pain unresponsive to conservative treatment for KOA. Visual Analog Scale (VAS) for knee pain were collected at baseline and 3 and 6 months post-treatment. Statistical analyses included paired t-tests, logistic regression, and one-way analysis of variance (ANOVA).</div></div><div><h3>Results</h3><div>A total of 2068 patients (2815 knees) were included. The average age was 65.2 years, and there were more female than male patients. PRP therapy significantly improved the VAS scores from 53.5 at baseline to 35.8 at 6 months (P &lt; 0.05). Patients with Kellgren-Lawrence (KL) grade 4 showed less improvement than those with KL grades 2 and 3. Blood neutrophil levels positively correlated with pain severity. VAS improvement negatively correlated with age and body mass index (BMI). No major adverse events were reported during the study period.</div></div><div><h3>Conclusion</h3><div>PRP therapy for KOA, as observed in the clinical setting in Japan, is a safe treatment option with significant pain reduction, particularly in patients with less severe deformity of KOA. However, its effectiveness decreases in severe deformity cases. These real-world clinical data provide valuable insights for refining the patient selection criteria and advancing KOA treatment strategies.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 427-434"},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma-derived exosomes stimulate hair follicle growth through activation of the Wnt/β-Catenin signaling pathway","authors":"Changpei Lu ,&nbsp;Yunbu Ding ,&nbsp;Rongshuang Zhang ,&nbsp;Yimei Du ,&nbsp;Lingbo Bi ,&nbsp;Min Zhao ,&nbsp;Chaofan Wang ,&nbsp;Qiaofang Wu ,&nbsp;Haixia Jing ,&nbsp;Weixin Fan","doi":"10.1016/j.reth.2025.04.003","DOIUrl":"10.1016/j.reth.2025.04.003","url":null,"abstract":"<div><h3>Background</h3><div>Androgenetic alopecia (AGA) is a common type of hair loss that affects a large segment of the global population, significantly influencing individuals' appearance and mental health. Existing treatments like minoxidil and finasteride have limited effectiveness and can cause side effects, highlighting the need for alternative therapies.</div></div><div><h3>Objective</h3><div>This study aims to explore the effectiveness of platelet-rich plasma-derived exosomes (PRP-Exos) in stimulating hair follicle growth and the proliferation of human dermal papilla cells (DPCs), as well as to investigate the mechanisms involved.</div></div><div><h3>Methods</h3><div>PRP-Exos were isolated and characterized using techniques such as nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and Western blotting. The impact of PRP-Exos on DPC proliferation was measured using CCK-8 assays, while their migration was assessed through Transwell migration and scratch wound healing assays. Flow cytometry was used to analyze cell cycle progression. Hair follicle organ culture was employed to examine the effects of PRP-Exos on hair follicle growth, and in vivo experiments were conducted in a mouse model to assess the influence of PRP-Exos on hair follicles.</div></div><div><h3>Results</h3><div>DPCs internalized PRP-Exos, which significantly boosted their proliferation and migration, as shown by CCK-8, Transwell migration, and scratch wound healing assays. Flow cytometry revealed that PRP-Exos facilitated cell cycle progression in DPCs. Furthermore, treatment with PRP-Exos resulted in increased levels of β-Catenin and Lef-1, along with decreased expression of SFRP1, indicating activation of the Wnt/β-Catenin pathway. Hair follicle organ culture indicated enhanced hair follicle growth and a prolonged anagen phase, delaying the transition to the telogen phase. In vivo studies demonstrated increased skin thickness, hair follicle diameter, and a favorable anagen-to-telogen ratio in mice, promoting hair growth during the telogen phase.</div></div><div><h3>Conclusions</h3><div>PRP-Exos show promise as a therapeutic option for AGA by stimulating hair follicle growth through the activation of the Wnt/β-Catenin signaling pathway. These findings suggest that PRP-Exos could enhance hair follicle regeneration both in vitro and in vivo.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 435-446"},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}