Regenerative Therapy最新文献

{"title":"Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins","authors":"Masaya Tsukamoto ,&nbsp;Chiaki Kawabata ,&nbsp;Kohei Shishida ,&nbsp;Takumi Yoshida ,&nbsp;Kazuto Kimura ,&nbsp;Kazuya Edamura ,&nbsp;Kikuya Sugiura ,&nbsp;Shingo Hatoya","doi":"10.1016/j.reth.2025.05.008","DOIUrl":"10.1016/j.reth.2025.05.008","url":null,"abstract":"<div><h3>Introduction</h3><div>Mesenchymal stem cells (MSCs) possess immunomodulatory potential and are used for cell therapy in both human and veterinary medicine. However, donor-derived MSCs have limited proliferative activities and variations, which restrict their clinical applicability. In contrast, induced pluripotent stem cells (iPSCs) can self-renew indefinitely and differentiate into the three germ layers. By exploiting these characteristics, iPSCs can differentiate into mesenchymal stem cells (iMSCs) and potentially overcome the limitations of donor-derived MSCs. In humans, the characteristics of iMSCs have been reported to vary depending on the differentiation strategy and cell origin of iPSCs. However, no studies have investigated the differentiation strategies and cell origins of canine iPSCs (ciPSCs) in relation to iMSC generation.</div></div><div><h3>Methods</h3><div>Canine embryonic fibroblast-derived iPSCs (CEF-iPSCs) were differentiated into iMSCs via the mesoderm or ectoderm, and their proliferative ability and the expression levels of CD34, CD44, CD45, and CD90 were assessed. We then applied the iMSC induction method via the ectoderm to other ciPSC lines, including canine dermal fibroblast-derived iPSCs (CDF-iPSCs), canine peripheral mononuclear cell-derived iPSCs (cPBMC-iPSCs), and canine urine-derived cell-derived iPSCs (cUC-iPSCs). We assessed their proliferation, marker expression, and ability to differentiate into tri-lineages and performed comparative analyses.</div></div><div><h3>Results</h3><div>IMSCs derived from CEF-iPSCs via the ectodermal lineage showed higher proliferative ability and expressed MSC markers at a higher rate than iMSCs generated via mesodermal induction. Notably, with the exception of CDF-iPSCs, iMSCs were successfully generated from other ciPSC lines via ectodermal lineages. These iMSCs exhibited proliferative activities over passage 10, expressed MSC markers, and demonstrated the ability to differentiate into tri-lineages. iMSCs derived from cUC-iPSCs exhibited the highest expression of CD90 compared to other iMSCs.</div></div><div><h3>Conclusions</h3><div>Highly proliferative iMSCs expressing a high rate of MSC markers can be obtained from cUC-iPSCs via ectodermal induction. Our study demonstrated that the differentiation strategy and cell origin of ciPSCs play crucial roles in the generation of iMSCs.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 112-122"},"PeriodicalIF":3.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical outcome of adding injection of freeze-dried platelet-derived factor concentrate to operative treatment accompanied by an early rehabilitation for Achilles tendon rupture in high-level athletes","authors":"Shota Morimoto,&nbsp;Futoshi Morio,&nbsp;Yuta Matsumae,&nbsp;Masashi Nakamura,&nbsp;Yoshitaka Nakao,&nbsp;Toshiya Tachibana,&nbsp;Tomoya Iseki","doi":"10.1016/j.reth.2025.05.007","DOIUrl":"10.1016/j.reth.2025.05.007","url":null,"abstract":"<div><h3>Background</h3><div>Achilles tendon ruptures (ATRs) often occur in competitive athletes. Various studies have been conducted to improve the treatment outcomes for ATRs, but the results remain unsatisfactory for athletes.</div></div><div><h3>Purpose</h3><div>To review our experiences of adding injections of freeze-dried platelet-derived factor concentrate (FD-PFC) to surgical treatment with an early rehabilitation for ATRs in high-level athletes.</div></div><div><h3>Methods</h3><div>We retrospectively analyzed 8 high-level athletes (Tegner activity score ≥8) with ATR, who received postoperative injection of FD-PFC in addition to surgical treatment with an early rehabilitation protocol between May 2019 and November 2021. In all patients, surgery was performed using the modified side-locking loop suture technique, and an early rehabilitation protocol was implemented postoperatively. The prepared FD-PFC was injected into the Achilles tendon suture site under ultrasound guidance at 4 weeks postoperatively. Evaluation of the clinical outcomes included the following factor: the American Orthopedic Foot and Ankle Society ankle-hindfoot scale (AOFAS) score measured preoperatively and at one year postoperatively, time to ability to perform 20 continuous double- and single-leg heel raises (DHR/SHR), time to return to the original sport, and complications related to surgery or FD-PFC injection. In addition, magnetic resonance imaging (MRI) was performed for all patients at 12 and 24 weeks postoperatively to evaluate Achilles tendon maturation.</div></div><div><h3>Results</h3><div>The 8 patients consisted of 7 males and one female athletes with a mean age of 28.5 ± 5.2 years (range, 23–39 years) at the time of surgery. All patients were high-level athletes with a mean Tegner activity score of 9.0 ± 0.5 (range, 8–10). The mean AOFAS score improved significantly from 36.0 ± 9.4 preoperatively to 96.8 ± 3.6 at one year postoperatively (p &lt; 0.001). The mean time to be able to perform 20 continuous DHR and 20 continuous SHR was 6.8 ± 0.8 weeks and 10.1 ± 1.6 weeks, respectively. All patients could return to their original sport at pre-injury levels with the mean time of 18.3 ± 2.7 weeks. Also, there were no complications related to surgery or injection. Furthermore, T2-weighted MRI showed the high signal intensity of the sutured tendon in 25 % (2 of 8 patients) at 12 weeks and 0 % (0 of 8 patients) at 24 weeks postoperatively.</div></div><div><h3>Conclusions</h3><div>The addition of FD-PFC injections to surgical treatment with an early rehabilitation protocol could provide satisfactory results for high-level athletes with ATRs, enabling them early return to their original sports at pre-injury level. This treatment option may be useful for athletes with ATRs.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 107-111"},"PeriodicalIF":3.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich fibrin-conditioned medium promotes osteogenesis of dental pulp stem cells through TGF-β and PDGF signaling","authors":"Yasuyuki Fujii ,&nbsp;Takashi Yoshida ,&nbsp;Ayaka Sato ,&nbsp;Mikiko Ikehata ,&nbsp;Ayano Hatori ,&nbsp;Daichi Chikazu ,&nbsp;Shahram Ghanaati ,&nbsp;Yoko Kawase-Koga","doi":"10.1016/j.reth.2025.05.006","DOIUrl":"10.1016/j.reth.2025.05.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Mesenchymal stem cells (MSCs), a key cell source for regenerative medicine, are conventionally cultured in human autologous serum (HAS) or fetal bovine serum (FBS). Platelet-rich fibrin-conditioned medium (PRF-CM) has emerged as a potential alternative to FBS, promoting the osteogenic differentiation of dental pulp stem cells (DPSCs) more efficiently than FBS. However, the molecular mechanisms underlying PRF-CM-induced osteogenesis in DPSCs remain unclear. Therefore, the aim of this study was to elucidate these mechanisms.</div></div><div><h3>Methods</h3><div>PRF-CM was prepared from peripheral blood samples collected from three healthy donors. DPSCs were derived from the dental pulp extracted from the third molars of three healthy donors. The mRNA expression patterns of DPSCs cultured in FBS or PRF-CM were compared using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) were analyzed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.</div></div><div><h3>Results</h3><div>The mean blood volume collected from the three donors was 51.3 mL, yielding a mean PRF-CM volume of 168 mL. RNA-Seq identified 2258 DEGs, and KEGG pathway and GO analysis revealed that PRF-CM upregulated biological activities associated with transforming growth factor beta (TGF-β) signaling and platelet-derived growth factor (PDGF) binding compared with FBS. Western blotting analysis revealed that pSMAD2/3 and phosphor-Akt (<em>p</em>-Akt) were upregulated in DPSCs treated with PRF-CM, indicating that PRF-CM upregulated TGF-β and PDGF signaling pathways. The addition of SB431542 (a TGF-β inhibitor) or imatinib mesylate (a PDGF inhibitor) to osteogenic differentiation medium with PRF-CM reduced alkaline phosphatase (ALP) activity and osteogenic marker gene expression in DPSCs, respectively.</div></div><div><h3>Conclusions</h3><div>PRF-CM activates TGF-β and PDGF signaling pathways, thereby promoting the osteogenic differentiation of DPSCs. Moreover, the volume of PRF-CM obtained was approximately three times greater than the blood sample volume. These findings suggest that PRF-CM could serve as an effective alternative to HAS or FBS for bone regeneration therapy using MSCs.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 100-106"},"PeriodicalIF":3.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144137934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem cell therapy for urethra and ureter tissue engineering: A systematic literature review on animal studies","authors":"Hamoon Baghaei ,&nbsp;Ali Faegh ,&nbsp;Shima Jahani ,&nbsp;Pooya Hekmati ,&nbsp;Masoumeh Majidi Zolbin","doi":"10.1016/j.reth.2025.05.001","DOIUrl":"10.1016/j.reth.2025.05.001","url":null,"abstract":"<div><div>The present study investigates the optimal stem cells for using in ureter and urethra tissue engineering by systematically reviewing the literature. Scopus, PubMed, and Embase databases were searched up to December 2024 to find original studies investigating ureter and urethra tissue engineering by seeding stem cells on the scaffolds in animal models, which resulted in the identification of 1191 studies. Finally, 26 studies were included. Eighteen and eight studies tried urethra and ureter tissue regeneration, respectively. Adipose-derived stem cells were the most commonly used for tissue engineering of the ureter and urethra, with 14 studies. Bone marrow-derived stem cells were involved in 5 studies, urine-derived in 3, muscle-derived stem/precursor cells in 3, and human amniotic membrane-derived mesenchymal stem cells in 1. Bladder acellular matrix, artery extracellular matrix, and small intestinal submucosa were non-synthetic seeded scaffolds that were used in 10 studies. However, studies used biomaterial and synthetic scaffolds, including Poly (<span>l</span>-lactide-co-caprolactone) nano yarn, Poly-glycolic acid, nanofiber, double-modified sulfated bacterial cellulose, and silk scaffold more frequently. Data demonstrated that adipose-derived, bone marrow-derived, urine-derived, and human amniotic membrane-derived stem cells have the capability of both urothelium and smooth muscle regeneration in urethra reconstruction. Furthermore, seeding bone marrow-derived stem cells can improve collagen and vessel formation. Also, this study showed that the muscle-derived SCs or precursor cells are able to regenerate urethra smooth muscle, but data are limited. Hypoxia precondition, subcutaneous or omental pre-incubation, fibroblast growth factor receptor Ⅱ overexpression, and mechanical extension stimulation were employed as boosting conditions. However, data about ureter tissue engineering are limited. Adipose-derived stem cells were the most frequently employed for ureter tissue regeneration, with appropriate outcomes for both urothelium and smooth muscle regeneration however, other types of stem cells should be more investigated for ureter tissue engineering.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 92-99"},"PeriodicalIF":3.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of mesenchymal stem cells and platelet-rich plasma therapies on wound healing: A Systematic Review and meta-analysis","authors":"Yunqing Sun ,&nbsp;Ming Zhu ,&nbsp;Lianmei Qiu","doi":"10.1016/j.reth.2025.04.010","DOIUrl":"10.1016/j.reth.2025.04.010","url":null,"abstract":"<div><h3>Background</h3><div>Wound healing is a complex physiological process essential for maintaining tissue integrity and function. This meta-analysis aims to evaluate the efficacy of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP), and their combination in enhancing wound healing.</div></div><div><h3>Methods</h3><div>We conducted a systematic search of PubMed, Web of Science, and Scopus databases for studies published from 2010 to 2024. The inclusion criteria were randomized controlled trials (RCTs) and controlled clinical trials involving human subjects treated with MSCs, PRP, or both. The primary outcomes assessed were wound closure rates and mean healing times, while secondary outcomes included wound size reduction, pain management, infection rates, recurrence, adverse events, and cost-effectiveness. Data were pooled using STATA software version 17.0, with standardized mean differences (SMDs) and risk differences (RDs) calculated.</div></div><div><h3>Results</h3><div>A total of 34 studies involving 2458 patients were included. PRP and MSCs significantly reduced wound healing time (PRP: SMD = −1.08, 95 % CI: 1.75 to −0.42, p &lt; 0.001; MSCs: SMD = −1.7195 %, CI: 2.44 to −0.99, p &lt; 0.001). MSCs positively impacted new vessel development (overall SMD = 0.55, 95 % CI: 0.39 to 0.71, p &lt; 0.001). PRP-treated groups had higher ulcer healing rates (72.4 % vs 52.5 %, RD = 0.21, 95 % CI: 0.16 to 0.26, p &lt; 0.001). Infection rates showed no statistically significant difference between PRP and control groups (DR = −0.11, 95 % CI: 0.34 to 0.12, p &gt; 0.05), while rest pain scores were significantly lower in PRP-treated patients (SMD = −4.69, 95 % CI: 0.87 to −0.62, p = 0.02). PRP-treated ulcers had lower recurrence rates (RD = −0.14, 95 % CI: 0.75 to 0.97, p = 0.01).</div></div><div><h3>Conclusions</h3><div>The findings from this meta-analysis underscore the promising potential of PRP and MSCs as effective therapeutic strategies for wound healing when used individually.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 75-91"},"PeriodicalIF":3.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A temperature-sensitive chitosan hydrogels loaded with nano-zinc oxide and exosomes from human umbilical vein endothelial cells accelerates wound healing","authors":"Lei Yu ,&nbsp;Zihao Dai ,&nbsp;Yuchen Huang ,&nbsp;Shuo Tang ,&nbsp;Lihong Zhou ,&nbsp;Xuying Zhao ,&nbsp;Xianfeng Que ,&nbsp;Rongfeng Shi ,&nbsp;Jin Zhou ,&nbsp;Jixuan Dong ,&nbsp;Feng Wang ,&nbsp;Yunjuan Gu","doi":"10.1016/j.reth.2025.04.020","DOIUrl":"10.1016/j.reth.2025.04.020","url":null,"abstract":"<div><div>The suboptimal therapeutic outcomes of diabetic foot ulcers (DFUs) represent a significant global challenge. In recent years, studies have indicated that novel dressings incorporating exosomes (Exos), nanomaterials, and hydrogels following debridement can synergistically promote tissue repair, which has been widely recognized as a promising emerging trend in the treatment of DFUs. In this study, a combination of zinc oxide nanoparticles (ZnO-NPs), Exos, and chitosan (CS) hydrogel (CS/ZnO-NPs@Exos) was applied to the full-thickness cutaneous defects in a diabetic rat model. This CS/ZnO-NPs@Exos hydrogel was applied to the wound site to achieve sustained and long-term release of Exos, allowing the evaluation of its therapeutic effects. This hydrogel significantly improved the wound closure rate in diabetic skin injuries and reduced oedema, erythema and inflammatory exudate at the wound site. These effects were characterized by enhanced re-epithelialization, reduced infiltration of inflammatory cells, increased collagen deposition, and enhanced angiogenesis in the wound area. This may be related to the Exos derived from human umbilical vein endothelial cells (HUVECs), which notably promote the migration and proliferation of fibroblasts. As a result, the CS/ZnO-NPs@Exos hydrogel offers a new therapeutic dressing for the management of diabetic wounds, with the potential to play a crucial role in clinical practice.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 63-74"},"PeriodicalIF":3.4,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping intellectual structures and research hotspots of chronic wound in global perspective","authors":"Yushu Zhu ,&nbsp;Yizhen Lin ,&nbsp;Sujie Xie ,&nbsp;Mingxuan Yang ,&nbsp;Wei Zhang ,&nbsp;Minjuan Wu ,&nbsp;Yifan Liu ,&nbsp;Dayuan Xu ,&nbsp;Shuyuan Xian ,&nbsp;Xirui Tong ,&nbsp;Jie Huang ,&nbsp;Luofeng Jiang ,&nbsp;Xinya Guo ,&nbsp;Minyi Gu ,&nbsp;Hengkai Yu ,&nbsp;Xinran Ding ,&nbsp;Yixu Li ,&nbsp;Yiyao Du ,&nbsp;Heng He ,&nbsp;Jianyu Lu ,&nbsp;Shizhao Ji","doi":"10.1016/j.reth.2025.05.002","DOIUrl":"10.1016/j.reth.2025.05.002","url":null,"abstract":"<div><h3>Background</h3><div>Chronic wounds included but were not limited to diabetes foot ulcers, venous leg ulcers and pressure ulcers. The challenge of difficult healing placed a heavy burden on patients and society. Our objective was to explain the healing process of chronic wounds and the development of treatment technologies in the past few years and to provide relevant, valuable information.</div></div><div><h3>Methods</h3><div>Our scientific publications were retrieved from the core collection of the Web of Science (WoSCC) database collection. The bibliometric visualization and analysis were performed by the software Biblioshiny based on R-bibliometrix. VOSviewer software and Citespace software were responsible for the validation of the results.</div></div><div><h3>Results</h3><div>A total of 8129 articles related to wound healing in chronic wounds were retrieved. The countries, institutions, and journals with the highest number of publications were the USA, the N8 research partnership, and the Journal of Wound Care, respectively. Armstrong DG and Dumville JC were the most influential authors in this field. The keyword analysis showed two key clusters of keywords, including “dressings” and “management”. Trend topics analysis revealed frequent keywords in recent years, including “nanofibers” and “injectable hydrogels”.</div></div><div><h3>Conclusion</h3><div>Our research was the first to reveal the cellular and molecular mechanisms and key clinical management strategies in the healing process of chronic wounds in the future through metrological and systematic evaluation, which may have important translational value in the future.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 47-62"},"PeriodicalIF":3.4,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface of the special issue “Mechanisms of reprogramming for cell differentiation and tissue regeneration”","authors":"Goshi Shiota (Senior Associate Editor)","doi":"10.1016/j.reth.2025.05.005","DOIUrl":"10.1016/j.reth.2025.05.005","url":null,"abstract":"","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Page 46"},"PeriodicalIF":3.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing nerve regeneration: A novel approach using polylactic acid/chitosan conduit with nerve-like cells and Bacopa monnieri in male rat model","authors":"Elham Hoveizi ,&nbsp;Hadi Naddaf ,&nbsp;Emad Naeimavi","doi":"10.1016/j.reth.2025.05.003","DOIUrl":"10.1016/j.reth.2025.05.003","url":null,"abstract":"<div><h3>Objectives</h3><div>This research aims to use neural-like cells (NLCs) derived from endometrial mesenchymal stem cells (EnMSCs) on a polylactic acid/chitosan scaffold (PLA/CS) along with the use of <em>bacopa monnieri</em> (Memocopa) in a rat sciatic nerve injury model for sciatic nerve regeneration. While previous studies have explored stem cell therapies and scaffold-based approaches for nerve regeneration, using EnMSCs in combination with a PLA/CS scaffold and Memocopa represents a novel, potentially synergistic approach.</div></div><div><h3>Method</h3><div>EnMSCs were isolated, characterized, and differentiated in a study. The expression of specific genes in the differentiated cells was confirmed using RT-PCR and immunocytochemistry. PLA nanofiber and chitosan hydrogel scaffolds were created for neural tissue engineering. Memocopa was administered orally alongside scaffold and cell transplantation. The study involved 25 adult male Wistar rats with a 3 mm sciatic nerve gap, divided into five groups based on treatment. Animals were monitored for 8 weeks, during which SFI was measured. Tissue samples were then prepared for histological examination, including various staining techniques.</div></div><div><h3>Results</h3><div>The combination of scaffold, cells, and Memocopa showed significant improvements in sciatic nerve function, as indicated by the SFI results in the eighth week: conduit group −33.87, conduit/cells group −25.92, conduit/cells/Memocopa group −22.86, Memocopa group −30.93, and control group −38.87. Histological findings revealed improvements in various aspects, including percentages of new nerve formation across the different treatment groups: conduit group 4.62 %, conduit/cells group 16.45 %, conduit/cells/Memocopa group 21.32 %, Memocopa group 7.07 %, and control group 0.22 %.</div></div><div><h3>Conclusions</h3><div>The results of this study showed that efficient differentiation of EnMSCs into NLCs is possible, and with the help of PLA/CS scaffold and simultaneous use of Memocopa, it is possible to repair and improve sciatic nerve injury in a rat animal model.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 31-45"},"PeriodicalIF":3.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet rich plasma promotes cell migration of adipose-derived stem cells by up-regulation of focal adhesion kinase and paxillin","authors":"Karis Chen ,&nbsp;Yen-Chia Chen ,&nbsp;Tung-Yang Yu ,&nbsp;Li-Ping Lin ,&nbsp;Hung-Chih Hsu ,&nbsp;Yu-Cih Liao ,&nbsp;Jong-Hwei Su Pang ,&nbsp;Wen-Chung Tsai","doi":"10.1016/j.reth.2025.05.004","DOIUrl":"10.1016/j.reth.2025.05.004","url":null,"abstract":"<div><div>Skeletal muscle injuries are prevalent in competitive sports, often disrupting elite athletes' training programs. Platelet-rich plasma (PRP) is abundant in growth factors, cytokines, and chemokines, making it a potential solution for treating muscle injuries. Adipose-derived stem cells (ADSCs) therapy presents promise for tissue regeneration, given their self-renew and differentiation capabilities. This study aims to investigate the impact of PRP on ADSCs, with a focus on cell migration. ADSCs were isolated from the subcutaneous adipose tissue of Sprague Dawley rats. Subsequently, these ADSCs were treated with PRP. The migration ability was assessed through in vitro wound assays, migration assays, and cell spreading assays. Molecular analysis, employing Western blot, was performed to examine the expression levels of phosphorylated focal adhesion kinase (FAK), FAK, phosphorylated paxillin, paxillin, and vinculin. The filamentous actin (F-actin) cytoskeleton formation was assessed through immunofluorescence staining. The study revealed a positive impact of PRP on ADSC migration in the in vitro wound assay, migration assay, and cell spreading assay. PRP elevated the expression levels of phosphorylated FAK, FAK, phosphorylated paxillin, paxillin, and vinculin in ADSCs. Additionally, PRP affected the distribution of F-actin in ADSCs. Furthermore, siRNA-mediated knockdown of FAK and paxillin confirmed their essential roles in PRP-enhanced ADSC migration. In conclusion, our findings demonstrated that PRP promoted the migration, spreading, in vitro wound healing, and cytoskeleton rearrangement of ADSCs by upregulating the expressions of phosphorylated FAK, FAK, phosphorylated paxillin, paxillin, and vinculin. This insight supports the potential use of PRP in combination with ADSCs for regeneration therapies.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 9-19"},"PeriodicalIF":3.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}