Regenerative Therapy最新文献

{"title":"Comprehensive bibliometric analysis and perspectives on therapies targeting colon cancer stem cells over a 40-year period","authors":"Şeyda Berk","doi":"10.1016/j.reth.2025.02.015","DOIUrl":"10.1016/j.reth.2025.02.015","url":null,"abstract":"<div><div>The presence of cancer stem cells (CSCs) is one of the primary causes of recurring therapy resistance because they have two main capacities: self-renewal and avoiding apoptotic pathways. Despite their relevance, no full bibliometric analysis has yet been done in this topic. The goal of this work is to use bibliometric analysis to map the fundamental and emergent areas in therapeutics targeting colon cancer stem cells. To perform bibliometric analysis on colon cancer stem cells (CCSCs) literature, spanning roughly the last 40 years, in order to establish a firm base for future projections by emphasizing the findings of the most notable research. All information pertinent to CCSCs was accessed from Web of Science Core Collection database. In order to identify and analyze the research hotspots and trends related to this topic, Biblioshiny (RStudio) and VOSviewer were utilized to ascertain the countries/regions, institutions, journals, authors, references, and keywords involved. The targeted time span covered 1735 research-, and review articles. The most frequent keywords were “colorectal cancer,” “cancer stem cells,” and “colon cancer,” while the most trending keywords in the last few years were “protein stability,” “spheroid formation,” “ubiquitination,” “exosomes,” “patient-derived organoids,” and “gut microbiota.” Over the past 40 years, there has been a significant advancement in researchers' understanding of colon cancer stem cells. In addition, the cluster map of co-cited literature showed that colon cancer stem cell research has emerged as a research hotspot. It was also anticipated that the main focus of the future efforts appears to involve clinical applications of cell-targeted colon cancer therapy. These results provide researchers with a comprehensive understanding of this field and provide insightful ideas for further research.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 19-34"},"PeriodicalIF":3.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidance on the clinical application of extracellular vesicles","authors":"Shuji Terai ,&nbsp;Motohiro Asonuma ,&nbsp;Ayuko Hoshino ,&nbsp;Masahiro Kino-oka ,&nbsp;Takahiro Ochiya ,&nbsp;Kiyoshi Okada ,&nbsp;Yoji Sato ,&nbsp;Yuki Takahashi ,&nbsp;Morikuni Tobita ,&nbsp;Atsunori Tsuchiya","doi":"10.1016/j.reth.2025.02.008","DOIUrl":"10.1016/j.reth.2025.02.008","url":null,"abstract":"<div><div>Therapies using extracellular vesicles (EVs), exosomes, or cell culture supernatants containing EVs or exosomes (referred to as “EV therapies” in this Guidance) have garnered an increasing amount of interest. However, pharmaceutical products containing EVs as their main active ingredient are yet to receive regulatory approval.</div><div>The “Basic points to consider regarding the preparation of extracellular vesicles and their clinical applications in Japan,” was announced by the Japanese Society for Regenerative Medicine (JSRM) on March 10, 2021, with a specific focus on exosomes among EVs to promote high safety standards in this evolving field and facilitate the application of EV clinically. The Scientific Committee of the Pharmaceuticals and Medical Devices Agency and the Japanese Society for Extracellular Vesicles have issued reports and statements regarding treatment, resulting in a growing momentum toward treatment in Japan.</div><div>This article summarized the basic items that should be recognized comprehensively for clinical practice in three categories: (1) risk profiling, (2) preparation (manufacturing) process and quality, and (3) verification of EVs checking items and effectiveness. This guideline will be revised in the future with technological innovations and new findings; nevertheless, it will serve as a guide for the development of treatments.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 43-50"},"PeriodicalIF":3.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDIT4 participates in high glucose-induced fibroblast-like synoviocytes overactivation and cartilage injury by regulating glycolysis","authors":"Shuo Qiang,&nbsp;Cheng Cheng,&nbsp;Yonghui Dong,&nbsp;Chao Tang,&nbsp;Jia Zheng,&nbsp;Yunke Liu","doi":"10.1016/j.reth.2025.02.017","DOIUrl":"10.1016/j.reth.2025.02.017","url":null,"abstract":"<div><h3>Objective</h3><div>More and more evidence show that diabetes is closely related to osteoarthritis (OA). However, the role and mechanism of DNA damage-inducible transcript 4 protein (DDIT4) in diabetic OA (DOA) have not been clarified.</div></div><div><h3>Methods</h3><div>We collected OA patients and non-OA subjects who underwent total knee replacement surgery, and analyzed the DDIT4 expression in synovial samples using RT-qPCR. The cell viability of fibroblast-like synoviocytes (FLSs) was measured by CCK-8 assay. Annexin V-FITC/PI double staining was used to detect the cell apoptosis. Scratch and Transwell assays were used to determine cell migration and invasion, respectively.</div></div><div><h3>Results</h3><div>The levels of cellular inflammatory factors (IL-1β, IL-6 and TNF-α), oxidative stress and glycolysis related indicators were detected by using kits. Western blot was used to determine the expression of DDIT4, Aggrecan, COL3A1, MMP3, MMP13, HK2, PFKP and PKM2 in FLSs or ATDC5 cells. The results showed that the expression level of DDIT4 was significantly reduced in the synovial samples of OA patients and primary FLSs. Functional studies showed that DDIT4 overexpression inhibited the overactivation, migration, and invasion of FLSs, as well as alleviated chondrocyte injury co-cultured with FLSs. Importantly, the expression of DDIT4 was down-regulated in patients with DOA and closely related to DOA. Further research found that high glucose (HG) promoted excessive activation, migration, and invasion of FLSs, and exacerbated the followed chondrocyte injury. Overexpression of DDIT4 alleviated HG-induced abnormal function of FLSs and injury to chondrocytes. Importantly, DDIT4 inhibited lactate synthesis, glucose uptake, LDH activity, extracellular acidification rate, oxygen consumption rate, and expression levels of glycolysis related protein (HK2, PFKP, PKM2) in HG-induced FLSs. And the glycolysis inhibitors (Cyto-B and 3BrPA) alleviated the injury of ATDC5 chondrocytes co-cultured with FLSs.</div></div><div><h3>Conclusions</h3><div>DDIT4 participates in HG-induced FLSs overactivation and inflammation response, as well as chondrocyte injury and OA progression by regulating glycolysis processes.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 51-59"},"PeriodicalIF":3.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of cell-based therapies in digestive disorders: Obstacles and opportunities","authors":"Guodong Liang ,&nbsp;Yuehan Ma ,&nbsp;Ping Deng ,&nbsp;Shufeng Li ,&nbsp;Chunyan He ,&nbsp;Haihang He ,&nbsp;Hairui Liu ,&nbsp;Yunda Fan ,&nbsp;Ze Li","doi":"10.1016/j.reth.2025.02.009","DOIUrl":"10.1016/j.reth.2025.02.009","url":null,"abstract":"<div><div>Stem cell-based therapies have emerged as a promising frontier in the treatment of gastrointestinal disorders, offering potential solutions for challenges posed by conventional treatments. This review comprehensively examines recent advancements in cell-based therapeutic strategies, particularly focusing on stem cell applications, immunotherapy, and cellular therapies for digestive diseases. It highlights the successful differentiation of enteric neural progenitors from pluripotent stem cells and their application in animal models, such as Hirschsprung disease. Furthermore, the review evaluates clinical trials and experimental studies demonstrating the potential of stem cells in regenerating damaged tissues, modulating immune responses, and promoting healing in conditions like Crohn's disease and liver failure. By addressing challenges, such as scalability, immunogenicity, and ethical considerations, the review underscores the translational opportunities and obstacles in realizing the clinical potential of these therapies. Concluding with an emphasis on future directions, the study provides insights into optimizing therapeutic efficacy and fostering innovations in personalized medicine for digestive disorders.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 1-18"},"PeriodicalIF":3.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the commercial manufacturing of tisagenlecleucel for patients in Japan: A 4-year experiential journey","authors":"Fumiko Iwamoto ,&nbsp;David Fong ,&nbsp;Margarida Rodrigues ,&nbsp;Yoshie Tsurumaki","doi":"10.1016/j.reth.2025.02.001","DOIUrl":"10.1016/j.reth.2025.02.001","url":null,"abstract":"<div><h3>Background</h3><div>Tisagenlecleucel, an autologous CD19-directed chimeric antigen receptor T-cell therapy, was approved in Japan, in March 2019 for patients with relapsed/refractory (r/r) B-cell acute lymphoblastic leukamia and r/r diffuse large B-cell lymphoma, and in August 2022 for patients with r/r follicular lymphoma. Post-approval, a key goal has been to upscale and continuously improve manufacturing and shipment success rates (MSR and SSR, respectively) in the commercial setting to meet the needs of patients worldwide, including in Japan. Herein, we report accrued experience from a 4-year journey of commercial tisagenlecleucel manufacturing process optimization for patients in Japan.</div></div><div><h3>Methods</h3><div>Data were collected from the Novartis tisagenlecleucel manufacturing database, and only commercial tisagenlecleucel orders between August 2019 and July 2023 were included. The data for cancellations after shipment were based on cancellation forms obtained from institutions.</div></div><div><h3>Results</h3><div>Since the first approval of tisagenlecleucel in Japan, its MSR and SSR improved over 4 years. Particularly, the MSR increased from 85.6 % in the first year to 95.3 % in the year 4, while the SSR increased from 92.4 % to 98.4 % in the same period. The corresponding termination and out-of-specification (OOS) rates decreased from 6.8 % to 0.9 %, and from 7.6 % to 3.7 %, respectively. The MSR (87.9 % versus 94.6 %), SSR (93.0 % versus 97.5 %), termination (6.0 % versus 1.9 %), overall OOS rates (6.1 % versus 3.5 %), and viability OOS (2.8 % versus 0.2 %) have improved significantly since the implementation of manufacturing process improvements in 2022.</div></div><div><h3>Conclusion</h3><div>Continuous improvements in the tisagenlecleucel manufacturing process over the years resulted in higher manufacturing success and increased product availability for Japanese patients.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 619-624"},"PeriodicalIF":3.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive and degradable collagen-based three-dimensional scaffold encapsulated with adipose mesenchymal stem cells-derived exosomes improved diabetic wound healing","authors":"Fereshteh Talebpour Amiri ,&nbsp;Melody Omraninava ,&nbsp;Shadman Shahzamani ,&nbsp;Amirali Khodashenas ,&nbsp;Arman Daryakar ,&nbsp;Davood Nasiry","doi":"10.1016/j.reth.2025.02.002","DOIUrl":"10.1016/j.reth.2025.02.002","url":null,"abstract":"<div><div>A diabetic wound is the most frequent form of chronic wound. Because diabetic wounds have multiple factors contributing to their development, the best treatments involve using a combination of approaches. Herein we assessed whether bioactive and degradable bioengineered micro-porous collagen-based three-dimensional scaffold (CTS) encapsulated with adipose mesenchymal stem cells (ASCs)-derived exosomes could accelerate the wound healing process in diabetic rats. Diabetic animals were assigned to the control group, CTS group, Exo group, and CTS+Exo group. Tissue samples were collected on days 7, 14, and 21 for evaluations including stereological, molecular, and tensiometrical assessments. The findings showed that in the treatment groups, there was a notably increase in wound closure rate, total volumes of newly formed epidermis and dermis, numerical densities of fibroblasts and blood vessels, collagen density, and biomechanical parameters than the control group, with the most noticeable changes seen in the CTS+Exo group. Additionally, there was a notably increase in the transcript of miRNA-146a, TGF-β, bFGF, and VEGF genes in the treatment groups than the control group, with the highest expression observed in the CTS+Exo group. In the CTS+Exo group, there was a much greater decrease in TNF-α and IL-1β expression, as well as in the number of neutrophils, compared to the other groups. These results validated that the combination of CTS and ASCs-derived exosomes has a greater effect on improving diabetic wound healing.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 606-618"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electro-spun piezoelectric PLLA smart composites as a scaffold on bone fracture: A review","authors":"Melika Mirghaffari ,&nbsp;Asal Mahmoodiyan ,&nbsp;Shahram Mahboubizadeh ,&nbsp;Atefeh Shahbazi ,&nbsp;Yaser Soleimani ,&nbsp;Sana Mirghaffari ,&nbsp;Zahra Shahravi","doi":"10.1016/j.reth.2025.01.026","DOIUrl":"10.1016/j.reth.2025.01.026","url":null,"abstract":"<div><div>The intrinsic ability of these scaffold materials to generate piezoelectric currents presents a major proposition for increasing the complexity of the scaffold and facilitating tissue healing. The piezoelectric behavior of bone tissue has been an area of interest for many researchers in the past. However, these properties have not been given much attention in new osteochondral tissue scaffold designs, whereas the established design factors mainly concentrate on the structural and mechanical characteristics of the tissue in question. The main advantages of the piezoelectric electrospun scaffolds in tissue engineering consist in the possibility to reproduce the piezoelectric properties of the fibrous extracellular matrix (ECM) of the tissue and the application of combined electrical and mechanic stimulation in the process of bone tissue regeneration. Poly-<span>l</span>-lactic acid (PLLA) has proved to be a potential biomaterial because of its adjustable mechanical characteristics and the bio-degradable capability for the creation of porous scaffolds with micro/nanostructure designs in various techniques. PLLA-based scaffolds can be altered on their surface or can be incorporated with other polymers either natural or synthetic or bioceramic materials. These modifications and combinations are to introduce improvement or changes in the scaffolds for the improvement of the functional properties that favor bone tissue engineering. In this review, we discussed the properties of PLLA and more particularly, the fracture-repairing activity in bone fracture therapy. It also investigates the interaction of PLLA with other biopolymers or biomaterials to dramatically enhance bone scaffolds' performance.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 591-605"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc sulfate gel reshapes the wound microenvironment to promote full-thickness wound healing in mice","authors":"Ying Zhang ,&nbsp;Xuebo Wei ,&nbsp;Yun Xu ,&nbsp;Weidong Xia ,&nbsp;ChaoYu Zheng ,&nbsp;Hongyu Zhang ,&nbsp;Weiting Chen ,&nbsp;Ke Xu ,&nbsp;Qun Huang","doi":"10.1016/j.reth.2025.01.022","DOIUrl":"10.1016/j.reth.2025.01.022","url":null,"abstract":"<div><div>Delayed healing of skin wounds significantly impacts human life, Zinc plays a pivotal role in human growth, the immune system, and various cellular processes. Previous studies have demonstrated that zinc supplementation or topical zinc ion therapy can accelerate wound healing in cases of skin injury. Zinc deficiency has been linked to delayed wound healing, but the role it plays remains to be elucidated. In this study, we report the preparation of zinc sulfate gel to promote wound healing by treating inflammation, antioxidant effects, and angiogenesis. We demonstrated the efficacy of zinc sulfate gel in wound healing in a mouse model of full-thickness skin excision, spared the anti-inflammatory and antioxidant capabilities of zinc ions. Furthermore, Zinc sulfate gel can stimulate tissue to enter the proliferative phase by regulating macrophage polarization, thereby accelerating collagen deposition, granulation tissue formation, and extracellular matrix production and remodeling. In conclusion, zinc ion gel provides a promising strategy for skin wounds regeneration.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 582-590"},"PeriodicalIF":3.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research status of biomaterials based on physical signals for bone injury repair","authors":"Qi Sun ,&nbsp;Chao-Hua Li ,&nbsp;Qi-Shun Liu ,&nbsp;Yuan-Bin Zhang ,&nbsp;Bai-Song Hu ,&nbsp;Qi Feng ,&nbsp;Yong Lang","doi":"10.1016/j.reth.2025.01.025","DOIUrl":"10.1016/j.reth.2025.01.025","url":null,"abstract":"<div><div>Bone defects repair continues to be a significant challenge facing the world. Biological scaffolds, bioactive molecules, and cells are the three major elements of bone tissue engineering, which have been widely used in bone regeneration therapy, especially with the rise of bioactive molecules in recent years. According to their physical properties, they can be divided into force, magnetic field (MF), electric field (EF), ultrasonic wave, light, heat, etc. However, the transmission of bioactive molecules has obvious shortcomings that hinder the development of the tissue-rearing process. This paper reviews the mechanism of physical signal induction in bone tissue engineering in recent years. It summarizes the application strategies of physical signal in bone tissue engineering, including biomaterial designs, physical signal loading strategies and related pathways. Finally, the ongoing challenges and prospects for the future are discussed.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 544-557"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovation in ovary decellularization methods: Chemical and herbal detergents","authors":"Mozafar Khazaei ,&nbsp;Sepideh Kadivarian ,&nbsp;Diyar Qadir Nabi ,&nbsp;Leila Rezakhani","doi":"10.1016/j.reth.2025.01.023","DOIUrl":"10.1016/j.reth.2025.01.023","url":null,"abstract":"<div><div>The ovary provides an ideal environment for egg survival due to its distinct structure that directly contributes to the growth and maintenance of the follicle. The purpose of this study is to compare ovarian decellularization with herbal and chemical detergents. Sheep ovarian was used in this study. 1 % sodium dodecyl sulfate (SDS) as a chemical detergent and 1, 2.5, and 5 % Acanthophyllum (ACP) were used as herbal agents for decellularization. DNA content, histological characteristics, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), biocompatibility, antibacterial test, hemocompatibility, and scanning electron microscope (SEM) were investigated. The results showed the DNA content in decellularization scaffolds with 1 % SDS and 5 % ACP was reduced suitably. Also, histological observations confirmed this finding, and the nuclei were completely removed in these two groups. Disorganization of collagen fibers and tissue architecture was observed more in the SDS group than in the ACP group. No group reported cytotoxicity and the best blood compatibility in decellularization with herbal agents was reported. Protein bands are largely conserved in all methods. Higher antibacterial properties were observed in the decellularization technique with ACP. Decellularization with 5%ACP, in addition to being able to completely remove cells in the tissue, can help preserve the ultrastructure of the ovary. Therefore, this plant agent can be introduced as a decellularization method for studies in this field.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 573-581"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}