Regenerative Therapy最新文献

{"title":"Mesenchymal stem cells: Guardians of women's health.","authors":"Guanwen Gao, Li Li, Changling Li, Degao Liu, Yunfei Wang, Changzhong Li","doi":"10.1016/j.reth.2024.10.011","DOIUrl":"https://doi.org/10.1016/j.reth.2024.10.011","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) have attracted more and more attention because of their multidirectional differentiation potential, immune regulatory abilities and self-renewal capacity. In recent years, their use has become prominent in the domains of regenerative medicine and tissue engineering. MSCs have shown promise in therapeutic studies for a variety of diseases and have become a new source of innovative solutions for the treatment of some obstetric and gynecological diseases. This review systematically presents the latest research on the use of MSCs in the treatment of obstetrics- and gynecology-related diseases. Specifically, this review encompasses the latest findings related to the role of MSCs in premature ovarian failure, polycystic ovary syndrome, ovarian cancer, fallopian tube-related diseases, uterine adhesions, endometriosis, cesarean scar defects, postmenopausal osteoporosis, and pelvic floor dysfunction. The shortcomings and challenges of the future use of MSCs in disease treatment are also discussed, with the intent to motivate improvements in MSC applications in clinical therapy. It is believed that with further research, MSCs will play a more important role in the treatment of obstetrics- and gynecology-related diseases.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1087-1098"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles originating from the mechanical microenvironment in the pathogenesis and applications for cardiovascular diseases.","authors":"Yu Zeng, Xiaodong Cui, Hong Li, Yanhui Wang, Min Cheng, Xiaoyun Zhang","doi":"10.1016/j.reth.2024.10.012","DOIUrl":"https://doi.org/10.1016/j.reth.2024.10.012","url":null,"abstract":"<p><p>The mechanical microenvironment plays a crucial regulatory role in the growth and development of cells. Mechanical stimuli, including shear, tensile, compression, and extracellular matrix forces, significantly influence cell adhesion, migration, proliferation, differentiation, and various other cellular functions. Extracellular vesicles (EVs) are involved in numerous physiological and pathological processes, with their occurrence and secretion being strictly regulated by the mechanical microenvironment. Recent studies have confirmed that alterations in the mechanical microenvironment are present in cardiovascular diseases, and the components of EVs can respond to changes in mechanical signals, thereby impacting the progression of these diseases. Additionally, engineered EVs, created by leveraging mechanical microenvironments, can serve as natural drug-delivery vehicles for treating and managing specific diseases. This article systematically reviews the regulatory mechanisms through which the mechanical microenvironment influences EVs and summarizes the role and advancements of EVs derived from this environment in the context of cardiovascular diseases.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1069-1077"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1.","authors":"Yin-Tzu Chen, Tai-Horng Young, Yu-Hsin Wang, Chih-Hsuan Huang, Yu-Yun Gao, Tsung-Wei Huang","doi":"10.1016/j.reth.2024.10.014","DOIUrl":"https://doi.org/10.1016/j.reth.2024.10.014","url":null,"abstract":"<p><strong>Introduction: </strong>Sensorineural olfactory dysfunction significantly impairs the life quality of patients but without effective treatments to date. Orexin is a neurotrophic factor activates neuronal network activity. However, it is still unknown whether orexin can promote differentiation in human olfactory sensory neurons (OSNs). This study seeks to explore the impact of orexin on the differentiation of human olfactory neuroepithelial cells (HONCs).</p><p><strong>Methods: </strong>The primary olfactory epithelium cells were cultured with or without orexin-A. The neural maturation-related and functional proteins were analyzed through immunofluorescence staining and Western blot. The function of HONCs were evaluated through the synaptic vesicle recycling assay.</p><p><strong>Results: </strong>The results showed that HONCs in the orexin-A group expressed higher levels of stage-specific markers, including achaete-scute homolog 1, βIII-tubulin, and olfactory marker protein. Additionally, more components of signaling transduction pathways compared to the control group. The orexin-A-mediated differentiation of OSN effect can be nullified with dual orexin receptor antagonist suvorexant and the selective orexin receptor type 1 antagonist SB674042, instead of selective orexin receptor type 2 antagonist TCS-OX2-29.</p><p><strong>Conclusions: </strong>Orexin-A elevates the expression of protein markers in human olfactory neuronal progenitor cells to stimulate the differentiation of OSN and enhances the formation of components of the olfactory-specific signaling transduction pathway via orexin receptor type 1.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1058-1068"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds.","authors":"Yu Yamazaki, Kaoruko Kikuchi, Yoko Yamada, Sakurako Neo, Suguru Nitta, Hirotaka Igarashi, Akihide Kamiya, Masaharu Hisasue","doi":"10.1016/j.reth.2024.09.003","DOIUrl":"https://doi.org/10.1016/j.reth.2024.09.003","url":null,"abstract":"<p><strong>Introduction: </strong>Exploring techniques for differentiating and culturing canine hepatocytes serves as a means to establish systems for liver transplantation and drug metabolism testing. However, establishing consistent methods for culturing stable hepatocytes remains a challenge. Recently, several investigations have shown the reprogramming of mature hepatocytes into hepatic progenitor cells by applying specific small molecule compounds, including Y-27632, (a ROCK inhibitor), A-83-01 (a TGFβ inhibitor), and CHIR99021 (a GSK3 inhibitor) (termed YAC) in rat, mouse, and humans, respectively. However, reports or evidence of successful reprogramming using these small-molecule compounds in dogs are absent. This study aimed to induce the differentiation of mature canine hepatocytes into progenitor cells.</p><p><strong>Methods: </strong>Cryopreserved canine hepatocytes (cHep) were cultured for 14 d in a YAC-supplemented hepatocyte growth medium. Subsequently, an assessment was conducted involving morphological observations, quantitative real-time polymerase chain reaction (qRT-PCR), and immunocytochemistry.</p><p><strong>Results: </strong>Notably, cryopreserved cHep cells emerged and exhibited ongoing proliferation and concurrently developed colonies within the YAC-enriched culture. These observations indicated that the mature hepatocytes reprogrammed into hepatic progenitor cells. Moreover, qRT-PCR analysis revealed a notable enhancement in gene expression levels. Specifically, the genes encoding α-fetoprotein (AFP), epithelial cell adhesion molecule (EpCAM), Cytokeratin 19 (CK19) and SRY-box9 (Sox9) displayed approximately 12-, 2.2-, 517- and 2.9- increases in hepatic progenitor cells, respectively, on day 14 as compared to their state before induction of differentiation. Hepatocyte-related protein expression of AFP, EPCAM, SOX9 and CK19 was confirmed via immunocytochemistry on day 21. In contrast, ALB and MRP2, which are highly expressed in mature hepatocytes, were decreased compared to those before YAC addition, which is consistent with the characteristics of undifferentiated hepatocytes.</p><p><strong>Conclusions: </strong>Herein, we effectively promoted the reprogramming of cryopreserved cHep cells into hepatic progenitor cells using three small-molecule compounds. The mRNA and protein expression analyses demonstrated increased levels of hepatic progenitor cells-specific markers, whereas markers related to mature hepatocytes decreased, suggesting that reprogramming cryopreserved cHep cells to hepatic progenitor cells was achieved using YAC. Therefore, cultivating liver progenitor cells holds the potential to offer valuable insights into the development of artificial livers for drug discovery research and transplantation therapy aimed at addressing liver diseases in dogs.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1078-1086"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scientometric and visualization analysis of 3D printing scaffolds for vascularized bone tissue engineering over the last decade.","authors":"Siyang Cao, Yihao Wei, Yaohang Yue, Deli Wang, Ao Xiong, Jun Yang, Hui Zeng","doi":"10.1016/j.reth.2024.10.013","DOIUrl":"https://doi.org/10.1016/j.reth.2024.10.013","url":null,"abstract":"<p><p>The introduction of three-dimensional (3D) printing scaffolds has emerged as an effective approach to achieving satisfactory revascularization for bone tissue engineering (BTE). However, there is a notable absence of analytical and descriptive investigations concerning the trajectory, essential research directions, current research scenario, pivotal investigative focuses, and forthcoming perspectives. Hence, the objective of this research is to offer a thorough overview of the advancements achieved in 3D printing structures for vascularized BTE within the last 10 years. Information extracted from the Web of Science repository spans from January 1, 2014, to April 1, 2024. Utilizing advanced analytical instruments, we conducted comprehensive scientometric and visual analyses. The findings underscore the predominant influence of China, representing 59.62 % of the overall publications and playing a pivotal role in shaping research within this field. Notable productivity was evident at various institutions, including Shanghai Jiao Tong University, Chinese Academy of Sciences, and Sichuan University. Wang Jinwu and Wu Chengtie stand out as the most prolific contributors in this domain. The highest number of publications in this area was contributed by the journal <i>Advanced Healthcare Materials</i>. In this study, osteogenesis imperfecta, osteosarcoma, fractures, osteonecrosis, and cartilage diseases were identified as the most significant disorders investigated in this research area. By providing a comprehensive scientometric assessment, this study benefits both experienced researchers and newcomers alike, offering prompt access to essential information and fostering the extraction of innovative concepts within this specific field.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1099-1116"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma-derived extracellular vesicles improve liver cirrhosis in mice.","authors":"Yuichirou Maeda, Yusuke Watanabe, Natsuki Ishikawa, Tomoaki Yoshida, Naruhiro Kimura, Hiroyuki Abe, Akira Sakamaki, Hiroteru Kamimura, Takeshi Yokoo, Kenya Kamimura, Atsunori Tsuchiya, Shuji Terai","doi":"10.1016/j.reth.2024.10.010","DOIUrl":"10.1016/j.reth.2024.10.010","url":null,"abstract":"<p><strong>Introduction: </strong>Cirrhosis remains a significant clinical challenge due to its poor prognosis and limited treatment options, creating a high unmet medical need for the development of novel therapies. In this study, we analyzed the effects of a novel approach to treat cirrhosis using platelet-rich plasma-derived extracellular vesicles (PRPEV) in mice.</p><p><strong>Methods: </strong>PRPEV were collected from platelet-rich plasma using ultrafiltration, and their proteomes were analyzed. The carbon tetrachloride (CCl₄)-induced cirrhosis model of mice was used to evaluate the effect of PRPEV administration and compared with the control group (n = 8). <i>In vitro</i> and <i>in vivo</i> mechanistic analyses of PRPEV administration were confirmed using real time-PCR and immunostaining.</p><p><strong>Results: </strong>Gene ontology analysis based on the proteome revealed that PRPEV contain many factors associated with EV and immune responses. <i>In vitro</i>, PRPEV polarize macrophages into an anti-inflammatory phenotype. Following PRPEV administration, there was a decrease in serum alanine aminotransferase levels and reduction in liver fibrosis, while mRNA levels of regenerative factors were upregulated and <i>transforming growth factor β-1</i> was downregulated<i>.</i> Furthermore, the number of anti-inflammatory macrophages in the liver increased.</p><p><strong>Conclusions: </strong>PRPEV may contribute to hepatocyte proliferation, anti-inflammation, and anti-fibrogenesis in the liver. This novel concept paves the way for cirrhosis treatment.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1048-1057"},"PeriodicalIF":3.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate-gelatin hydrogel promotes the neurogenic differentiation potential of bone marrow CD117⁺ hematopoietic stem cells.","authors":"Jinshan Peng","doi":"10.1016/j.reth.2024.10.009","DOIUrl":"10.1016/j.reth.2024.10.009","url":null,"abstract":"<p><p>People still hold the concept of using cell-based treatments to regenerate missing neurons in high esteem. CD117⁺ cells are considered favorable stem cells for regenerative medicine. The objective of this research was to examine the impact of Alginate-Gelatin (Alg-Gel) hydrogel on the process of neurogenic differentiation of CD117⁺ cells utilizing a cytokines secretion test conducted in a laboratory setting. To achieve this objective, bone marrow-CD117⁺ cells were isolated using the MACS technique and then transformed into neuron cells using a neurogenic differentiation medium. The characterization of enriched CD117⁺ cells has been done with flow cytometry as well as immunocytochemistry. Next, the cells underwent western blotting assay to evaluate the signaling pathways. Subsequently, the culture media was obtained from both groups in order to determine cytokine levels. The study revealed that the Alg-Gel hydrogel had a notable impact on enhancing the protein expression of neuron markers such as β-tubulin and Wnt/catenin signaling pathway components in CD117⁺ neurogenic differentiated cells. Furthermore, the cultured medium from the experimental group exhibited a notable abundance of IL-6 and IL-10 in comparison to the control group. The observed <i>in vitro</i> effects of Alg-Gel hydrogel on neurogenic differentiation of CD117⁺ cells are likely to be caused by the cytokines that are released.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1030-1036"},"PeriodicalIF":3.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promising nanotherapeutics of stem cell extracellular vesicles in liver regeneration.","authors":"Na Guo, Yan Wang, Zhaofeng Wen, Xiaofei Fan","doi":"10.1016/j.reth.2024.09.016","DOIUrl":"10.1016/j.reth.2024.09.016","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) have gainedsignificant attention due totheir crucialroles invarious biological systems. This review aims to explore the functions of EVs in both in physiological and pathological states of the liver, with a specific focus on the potential mechanisms and concrete evidence of EVs in liver regeneration processes. The review begins by emphasizing the importance of EVs in maintaining liver health and their involvement in different pathological conditions, starting from the liver's own EVs. Reviewing the role of EVs in liver diseases to reveal the impact of EVs in pathological processes (e.g., hepatitis, liver fibrosis, and cirrhosis) and elucidate their signaling functions at the molecular level. Subsequently, the work concentrates on the functions of EVs in liver regeneration, revealing their key role in repair and regeneration following liver injury by carrying growth factors, nucleic acids, and other bioactive molecules. This part not only theoretically clarifies the mechanisms of EVs in liver regeneration but also experimentally demonstrates their role in promoting liver cell proliferation, inhibiting apoptosis, regulating immune responses, and fostering angiogenesis, laying the groundwork for future clinical applications. Moreover, this work provides a comprehensive analysis of the challenges faced by existing EV-based therapies in liver regeneration and offers prospects for future research directions. It highlights that despite the tremendous potential of EVs in treating liver diseases, there are still technical challenges (e.g., EV isolation and purification, dosage control, and targeted delivery). To overcome these challenges, the review suggests improvements to current technologies and the development of new methods to realize the clinical application of EVs in treating liver diseases.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1037-1047"},"PeriodicalIF":3.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care.","authors":"Xin Liu, Shengwei Peng, Yongju Pei, Yuanyuan Huo, Yadi Zong, Jianwei Ren, Jing Zhao","doi":"10.1016/j.reth.2024.10.003","DOIUrl":"10.1016/j.reth.2024.10.003","url":null,"abstract":"<p><p>Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This research work demonstrates to use of solvent diffusion method to develop hydrogel nanocomposites composed of chitosan (CS), hyaluronic acid (HA), gold (Au), and fibroblast growth factors (FGF). The biological analysis of nanocomposites exhibited enhanced wound healing efficiency by incorporating bioactive molecules like FGF and bioactive Au nanoparticles. <i>In vitro,</i> cell compatibility analysis (MTT assay) of prepared hydrogel nanocomposites was studied on fibroblast cell lines NIH-3T3-L1 and L929 and exhibited greater cell survival ability (>90 %), cell proliferation and migration ability, which demonstrated the suitability of nanocomposite for wound healing treatment. <i>In vitro,</i> anti-bacterial analyses established that FGF-Au@CS/HA has strong antibacterial effectiveness against gram-positive and gram-negative pathogens. The observation of the present research revealed that prepared FGF-Au@CS/HA hydrogel composites could be a suitable biomaterial for diabetic wound care, potentially improving its antibacterial and healing efficacies.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1018-1029"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the superior function of RADA in bone regeneration compared to KSL as two critical cores within self-assembling peptide nanofibers: Insights from in vitro and in vivo studies.","authors":"Bita Rasoulian, Zahra Sheikholislam, Mohammad Hassan Houshdar Tehrani, Solmaz Chegeni, Elham Hoveizi, Seyed Mahdi Rezayat, Shima Tavakol","doi":"10.1016/j.reth.2024.09.010","DOIUrl":"10.1016/j.reth.2024.09.010","url":null,"abstract":"<p><strong>Introduction: </strong>Self-assembling peptide nanofibers have emerged as promising biomaterials in the realm of bone tissue engineering due to their biocompatibility, biodegradability, and ability to mimic the native extracellular matrix. This study delved into the comparative efficacy of two distinct self-assembling peptide nanofibers, RADA-BMHP1 and KSL-BMHP1, both incorporating the biological motif of BMHP1, but differing in their core peptide sequences.</p><p><strong>Methods: </strong>Cell viability and osteogenic differentiation in rat mesenchymal stem cells (rMSCs), and bone regeneration in rat were compared.</p><p><strong>Results: </strong>In vitro assays revealed that KSL-BMHP1 promoted enhanced cell viability, and nitric oxide production than RADA-BMHP1, an effect potentially attributable to its lower hydrophobicity and higher net charge at physiological pH. Conversely, RADA-BMHP1 induced superior osteogenic differentiation, evidenced by upregulation of key osteogenic genes, increased alkaline phosphatase activity (ALP), and enhanced matrix mineralization which may be attributed to its higher protein-binding potential and grand hydropathy, facilitating interactions between the peptide nanofibers and proteins involved in osteogenesis. In vivo experiments utilizing a rat bone defect model demonstrated that both peptide nanofibers improved bone regeneration at the genes level and ALP activity, with RADA-BMHP1 exhibiting a more pronounced increase in bone formation compared to KSL-BMHP1. Histological evaluation using H&E, Masson's trichrome and Wright-Giemsa staining confirmed the biocompatibility of both nanofibers.</p><p><strong>Conclusion: </strong>These findings underscore the pivotal role of the core structure of self-assembling peptide nanofibers, beyond their biological motif, in the fate of tissue regeneration. Further research is warranted to optimize the physicochemical properties and functionalization of these nanofibers to enhance their efficacy in bone regeneration applications.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"999-1009"},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}