Regenerative Therapy最新文献

{"title":"The effect of l-lactide-ε-caprolactone copolymer membrane on alveolar bone preservation: A comparative study using the open healing approach","authors":"Masayuki Takabayashi ,&nbsp;Yoshio Yahata ,&nbsp;Keisuke Handa ,&nbsp;Moe Sandar Kyaw ,&nbsp;Venkata Venkataih Suresh ,&nbsp;Masahiro Saito","doi":"10.1016/j.reth.2025.04.007","DOIUrl":"10.1016/j.reth.2025.04.007","url":null,"abstract":"<div><h3>Introduction</h3><div>This study evaluated the efficacy of a resorbable <span>l</span>-lactide-ε-caprolactone copolymer (PLCL) membrane in promoting epithelial healing and preserving alveolar bone morphology using the open healing approach (OHA), compared to conventional membranes.</div></div><div><h3>Methods</h3><div>Extraction sockets in beagle dogs were filled with bone substitute material and covered with different membranes. Healing was assessed via histological analysis and micro-CT imaging.</div></div><div><h3>Results</h3><div>The PLCL membrane reduced bone resorption and preserved alveolar bone morphology better than conventional membranes. Histological analysis showed excellent epithelial healing and notable bone formation.</div></div><div><h3>Conclusions</h3><div>The PLCL membrane effectively prevents infection, supports epithelial healing, and preserves alveolar bone morphology in OHA procedures.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 20-30"},"PeriodicalIF":3.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transplantation of chemically induced liver progenitors in Nagase analbuminemic rats under liver regenerative stimulus","authors":"Masayuki Fukumoto,&nbsp;Akihiko Soyama,&nbsp;Daisuke Miyamoto,&nbsp;Takanobu Hara,&nbsp;Hajime Matsushima,&nbsp;Hajime Imamura,&nbsp;Mampei Yamashita,&nbsp;Tomohiko Adachi,&nbsp;Kengo Kanetaka,&nbsp;Susumu Eguchi","doi":"10.1016/j.reth.2025.04.015","DOIUrl":"10.1016/j.reth.2025.04.015","url":null,"abstract":"<div><div>We have explored chemically induced liver progenitors (CLiP) as a potential therapeutic agent for liver diseases because their proliferative capacity <em>in vitro</em>. Therefore, we hypothesized the potential treatment with CLiP transplantation (Tx) could be effective and be enhanced with liver regenerative stimulus for metabolic liver diseases.</div></div><div><h3>Methods</h3><div>Male Sprague Dawley rats were used for mature hepatocytes (HEP) isolation to induce CLiP using 3 small molecules cocktail (ROCK-inhibitor, TGF beta-1 inhibitor, GSK3 inhibitor). Harvested rat CLiP (1 × 10⁶) were transplanted to the posterior lobe of the liver (30 % of whole liver) of Nagase analbuminemic rats (NAR) through portal vein injection. One week later, portal venous branch to non-transplanted lobe was ligated to induce liver regeneration in CLiP bearing liver lobe (portal branch ligation: PBL). Only CLiP Tx or HEP Tx of same dose were used as control groups. Serial measurement of serum albumin level was measured by ELISA method. All NARs were subject to immunosuppression therapy using tacrolimus s.q. 3 days per week.</div></div><div><h3>Results</h3><div>After HEP Tx especially with PBL, serum albumin level was significantly elevated. However, even with PBL, CLiP Tx group did not show significant increase in serum albumin. CLiP were surrounded by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) positive cells in portal veins suggesting that CLiP were exposed to the risk of innate immunity.</div></div><div><h3>Conclusion</h3><div>Although CLiP respond to growth factors and proliferate better than HEP <em>in vitro</em>, their behavior <em>in vivo</em> in response to liver regenerative stimulus were not similar to <em>in vitro</em> probably because of less immaturity of receptors and/or high immunogenicity as compared to HEP.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 1-8"},"PeriodicalIF":3.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive and biodegradable hydrogel based on the dermal matrix loaded with microspheres containing vascular endothelial growth factor (VEGF) and interleukin (IL)-10 accelerated the healing of diabetic wounds in rats","authors":"Zihan Wang ,&nbsp;Jia Liu ,&nbsp;Runpei Deng ,&nbsp;Yang Yang ,&nbsp;Chen Fangfang ,&nbsp;Shi Yonghui","doi":"10.1016/j.reth.2025.04.017","DOIUrl":"10.1016/j.reth.2025.04.017","url":null,"abstract":"<div><div>Diabetic wounds are among the most common and costly complications in diabetic patients. As a result, numerous studies have been conducted to explore effective approaches for accelerating the wound healing process. Biological hydrogels are frequently used for wound healing due to their favorable properties compared to other materials. In this study, we evaluated the effect of a bioactive and degradable hydrogel based on dermal matrix (HDM) encapsulated with microspheres containing vascular endothelial growth factor (VEGF) and interleukin (IL)-10 on promoting wound healing in diabetic rats. Forty-five diabetic rats were randomly assigned to three groups (n = 15): control, HDM, and HDM encapsulated with microspheres containing VEGF and IL-10 (HDMM). In addition, non-diabetic untreated rats (healthy group) were considered as control (n = 15). Wound assessments were performed on days 7, 14, and 21. The results demonstrated significant improvements in wound closure rate, fibroblast and blood vessel counts, collagen density, concentration levels of TGF-β and VEGF cytokines, and biomechanical parameters in the treatment groups compared to the control group, with the most pronounced effects observed in the HDMM group. Additionally, the HDMM group exhibited a greater reduction in neutrophil and macrophage counts, as well as decreased levels of TNF-α and IL-1β cytokines compared to the other groups. In conclusion, the HDM loaded with microspheres containing VEGF and IL-10 showed a more significant effect in promoting the healing of diabetic wounds.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 575-586"},"PeriodicalIF":3.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of repaglinide-embedded chitosan hydrogel in promoting wound healing","authors":"Chou-Yi Hsu ,&nbsp;Eman Raeed Azzam ,&nbsp;Mohammed Merza ,&nbsp;Marwah Suliman Maashi ,&nbsp;Ahmed Hjazi ,&nbsp;Rekha M.M. ,&nbsp;Subhashree Ray ,&nbsp;Kapil Ghai ,&nbsp;Udaybir Singh ,&nbsp;Narges Fereydouni","doi":"10.1016/j.reth.2025.04.021","DOIUrl":"10.1016/j.reth.2025.04.021","url":null,"abstract":"<div><h3>Introduction</h3><div>This study explores the potential of repaglinide-loaded chitosan-based hydrogels (Hy-R1% and Hy-R3%) as multifunctional scaffolds for wound healing applications. It aims to evaluate their physicochemical properties, cytocompatibility, antibacterial activity, and in vivo healing potential.</div></div><div><h3>Methods</h3><div>The hydrogels were prepared by dissolving chitosan in acetic acid, followed by the incorporation of repaglinide. In vitro assessments, including morphology, swelling, degradation, cytocompatibility, and antibacterial activity, were performed. In vivo wound healing was tested in a rat model using full-thickness wounds.</div></div><div><h3>Results</h3><div>Hy-R3% exhibited superior properties in surface morphology, swelling behavior, and degradation rate compared to the control. It also demonstrated the highest cell viability and significant antibacterial activity against <em>Staphylococcus aureus</em>. In vivo, Hy-R3% accelerated wound closure, showed superior re-epithelialization, and improved collagen deposition. Histological analysis confirmed better tissue remodeling and minimal inflammation in Hy-R3%.</div></div><div><h3>Conclusions</h3><div>Repaglinide-loaded chitosan-based hydrogels, particularly Hy-R3%, represent a promising new therapeutic approach for wound healing. The formulation demonstrated enhanced physicochemical and biological properties, including rapid wound closure and improved tissue regeneration. These findings suggest that repaglinide can be repurposed for wound healing applications, particularly for chronic and diabetic wounds, and lay the foundation for future studies on its broader regenerative potential.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 551-562"},"PeriodicalIF":3.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Historical evolution, hotspots, and trends in tendon tissue engineering: A bibliometric analysis","authors":"Wenlong Hou ,&nbsp;Wenwen Liu ,&nbsp;Mengtian Tan ,&nbsp;Jiaxu Fang ,&nbsp;Keye Cao ,&nbsp;Xincheng Du ,&nbsp;Haijun Zhang","doi":"10.1016/j.reth.2025.04.009","DOIUrl":"10.1016/j.reth.2025.04.009","url":null,"abstract":"<div><h3>Introduction</h3><div>As a significant branch of tissue engineering, tendon tissue engineering has developed rapidly, driven by advancements in new technologies and the refinement of medical theories. This study summarizes its development by analyzing historical evolution, research hotspots, and future trends to reveal core research directions and potential pathways.</div></div><div><h3>Methods</h3><div>Based on literature from WoS CC (1991–2024) and analyzed using VOSviewer, CiteSpace, and Bibliometrix, the study evaluates publication trends and research patterns.</div></div><div><h3>Results</h3><div>Findings show continuous growth in annual publications. The United States and China are key contributors with close collaboration. Chang James is the most prolific author, and stable research teams have emerged, though inter-team cooperation remains limited. Acta Biomaterialia publishes the most papers in this field, characterized by interdisciplinary integration. Key research focuses include scaffolds, seed cells, and rotator cuff injuries. Frontiers involve ideal scaffold construction, tendon adhesion prevention, and regeneration strategies. Future directions emphasize novel biomaterials, tendon-bone interface healing, innovative seed cell cultivation, tendon growth microenvironment construction, and clinical translation of research outcomes.</div></div><div><h3>Conclusion</h3><div>This bibliometric analysis reveals the core research directions and potential pathways in tendon tissue engineering, providing a reference for future studies.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 600-612"},"PeriodicalIF":3.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing intestinal disease research using gut-on-a-chip","authors":"Fuki Yokoi ,&nbsp;Sayaka Deguchi ,&nbsp;Kazuo Takayama","doi":"10.1016/j.reth.2025.04.023","DOIUrl":"10.1016/j.reth.2025.04.023","url":null,"abstract":"<div><div>The intestine is exposed constantly to mechanical stimuli, including blood flow or peristalsis. Recently, gut-on-a-chip models have been developed to recapitulate these physiological stresses. In such models, blood flow and peristalsis are simulated by perfusing culture medium through microchannels and applying cyclic suction to vacuum chambers. With these gut-on-a-chips, the effects of mechanical stimuli on the intestinal structure and function have been elucidated. Additionally, gut-on-a-chip models are used to study intestinal diseases, such as infectious diseases, inflammatory bowel disease, and colorectal cancer. Because they accurately recapitulate the pathophysiology of intestinal diseases, research leveraging their use has revealed mechanisms underlying disease onset and progression in the intestine. In this review, we summarize the development of gut-on-a-chip systems and highlight recent research on intestinal diseases using these models.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 541-550"},"PeriodicalIF":3.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis of hydrogels in bone regeneration: Research trends and future perspectives","authors":"Alimujiang Abudourousuli ,&nbsp;Aikebaierjiang Aisaiti ,&nbsp;Kan Jiang ,&nbsp;Shalayiding Aierxiding ,&nbsp;Kutiluke Shoukeer ,&nbsp;Ran Han ,&nbsp;Aikeremujiang Muheremu","doi":"10.1016/j.reth.2025.04.006","DOIUrl":"10.1016/j.reth.2025.04.006","url":null,"abstract":"<div><h3>Objective</h3><div>To analyze the research status and development prospects of hydrogels in the field of bone regeneration based on bibliometric methods.</div></div><div><h3>Methods</h3><div>In the Web of Science Core Collection database, a total of 1382 representative articles related to the application of hydrogels in bone regeneration were selected as of the end of 2023. Utilizing the tools VOSviewer and CiteSpace, an in-depth bibliometric visualization analysis was conducted to reveal the countries, authors, core literature, and journals that are highly productive in this field. Additionally, the research trends and development prospects within this area were analyzed.</div></div><div><h3>Results</h3><div>This study uses VOSviewer and CiteSpace to analyze 1382 highly cited articles on bone regeneration and materials science, revealing key trends and the evolution of the field. The United States, China, South Korea, Germany, the UK, and Japan are the leading countries in scientific output, with strong collaboration networks. Major research institutions, particularly in China and Japan, focus on hydrogels for bone regeneration. Top journals include <em>International Journal of Biological Macromolecules</em> and <em>Acta Biomaterialia</em>. Key authors include Timothy E. L. Douglas, known for developing mineralization-enhanced hydrogels, and Robert E. Guldberg, focused on growth factor delivery systems. The field has shifted from simple filling materials to multifunctional composites, with advancements in gene editing, signaling molecules, and bone regeneration drugs. Future trends include smart hydrogels, 3D printing, bioactive factor integration, cell engineering, and optimizing biodegradability and biocompatibility. Multifunctional composite hydrogels are expected to become the go-to materials for clinical bone repair.</div></div><div><h3>Conclusion</h3><div>Advancements in technology have transformed hydrogels from simple fillers to multifunctional composites, playing a crucial role in bone regeneration. Future trends include smart hydrogels, 3D printing, bioactive factor integration, cell engineering, and optimized biodegradability and biocompatibility. These developments indicate hydrogels will become the preferred material for clinical bone defect repair, significantly enhancing bone tissue repair and regeneration.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 587-599"},"PeriodicalIF":3.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in sciatic nerve regeneration: A review of contemporary techniques","authors":"Sardar Ali ,&nbsp;Ming Sun ,&nbsp;Muhammad Nadeem Khan ,&nbsp;Fang Qiang","doi":"10.1016/j.reth.2025.04.016","DOIUrl":"10.1016/j.reth.2025.04.016","url":null,"abstract":"<div><div>Sciatic nerve injury, affecting the longest and thickest nerve in the human body, often leads to severe pain, weakness, and impaired motor function in the lower extremities. Despite the peripheral nervous system's inherent capacity for some degree of regeneration, complete recovery remains elusive, necessitating advanced therapeutic approaches. This review explores two promising modalities electrical stimulation (ES) and platelet-rich plasma (PRP) that have shown the potential to enhance nerve repair and functional recovery. ES, through techniques such as transcutaneous electrical nerve stimulation (TENS), neuromuscular electrical stimulation (NMES), and direct current stimulation (DCS), facilitates neuronal regeneration by guiding axonal growth, releasing neurotrophic factors, and promoting synaptic plasticity. PRP, derived from autologous blood, is rich in growth factors such as Platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and nerve growth factor (NGF), which are essential for nerve regeneration, angiogenesis, and reducing inflammation. Clinical evidence supports the efficacy of ES and PRP in promoting nerve regeneration and functional recovery (Figure 1). However, further research is needed to optimize their application and understand their long-term outcomes. This review highlights the potential of these therapies to capitalize on their actions, potentially creating a robust regenerative milieu. Further research is needed to optimize treatment procedures and validate their efficacy and safety in humans.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 563-574"},"PeriodicalIF":3.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventing MSC aging and enhancing immunomodulation: Novel strategies for cell-based therapies","authors":"Faranak Elmi ,&nbsp;Fatemeh Soltanmohammadi ,&nbsp;Tahura Fayeghi ,&nbsp;Safar Farajnia ,&nbsp;Effat Alizadeh","doi":"10.1016/j.reth.2025.04.014","DOIUrl":"10.1016/j.reth.2025.04.014","url":null,"abstract":"<div><div>The efficacy of mesenchymal stem cells (MSCs) mediated regenerative therapies has been hindered by the senescence of them during long period cultures. Aged MSCs exhibit altered morphology, decreased stemmas, changed intercellular communication, and poor differentiation ability. Besides in physiological condition, upon transplantation of senescent MSCs, they are capable of activating both the innate and adaptive immune systems, playing a crucial role in preserving tissue homeostasis. Therefore, enhancing immunomodulation properties and preventing aging progress of MSCs to achieve successful future clinical applications seems necessary. This review delves into the current knowledge of the underlying cellular and molecular mechanisms that promote MSCs senescence as well as the developed approaches for reversing or preventing MSCs aging. These include pre-treatment of MSCs with various types of molecules to inhibit aging process and implementation of different types of three-dimensional culture systems. In addition, the recently developed strategies to improve immunomodulatory properties of MSCs have been discussed. By addressing the limitations of aged MSCs and augmenting their immunomodulation, these approaches offer a promising avenue for the future of cell therapy and provide valuable tools for maximizing the effectiveness of MSCs therapy in biomedical applications.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Pages 517-539"},"PeriodicalIF":3.4,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Platelet-rich plasma therapy for knee osteoarthritis: Insights from real-world clinical data in Japan” [Regen Ther 29 (2025) 427–434]","authors":"Yoshitomo Saita","doi":"10.1016/j.reth.2025.04.022","DOIUrl":"10.1016/j.reth.2025.04.022","url":null,"abstract":"","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"29 ","pages":"Page 540"},"PeriodicalIF":3.4,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}