Regenerative Therapy最新文献

{"title":"HUC-MSCs combined with platelet lysate treat diabetic chronic cutaneous ulcers in Bama miniature pig.","authors":"Yunyi Gao, Lihong Chen, Yan Li, Shiyi Sun, XingWu Ran","doi":"10.1016/j.reth.2024.11.005","DOIUrl":"10.1016/j.reth.2024.11.005","url":null,"abstract":"<p><p>Human umbilical cord mesenchymal stem cells (HUC-MSCs) and platelet lysate (PL) shows potential of wound healing. However, MSCs in combination with PL for wound healing is still lacking. In this study, we presented high glucose cultured wound related cells to mimic diabetic chronic ulcers (DCU) cells, wound healing indicators and the TGFβ/Smad signaling pathway were detected by PL cultured HUC-MSC supernatant (MSC-Sp) in vitro. In vivo study, diabetes was induced in pigs feeding a high-energy diet and multiple injections of streptozotocin (125 mg/kg). Chronic wounds were created on both sides of the backs of seven pigs by surgical creation and foreign body compression for eight weeks before treatment. The wounds were treated with saline control (N = 11), PL (N = 11), HUC- MSCs (N = 18, 6 × 10⁶/mL/cm²), and PL + HUC-MSCs (N = 18, 6 × 10⁶/mL/cm²) respectively. Tissue samples were collected to observe new collagen, neovascularization, wound healing factors, and the TGFβ/Smad signaling pathway. The resulting PL-cultured MSC-Sp promoted the proliferation of keratinocytes, fibroblasts, and vascular endothelial cells and inhibited the TGFβ1/TGFβ3 ratio, upregulated VEGF-α and PDGF-BB production by keratinocytes and fibroblasts, and downregulated the expression of CD86, IL-6, and TNF-α in RAW264.7 cells. PL + HUC-MSCs had the best wound healing rate in vivo, and promoted collagen formation, neovascularization, and inflammation, regulated the balance between IL-6/TGFβ1 and IL-6/Arg-1 and upregulated the expression of VEGF-α and TGFβ1. In summary, PL + HUC-MSCs had a better wound healing effect than HUC-MSCs or PL treatment alone by regulating the IL-6/Arg-1 and IL-6/TGFβ1 balance and upregulating TGFβ1, VEGF-α, Col1, and α-SMA.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1138-1149"},"PeriodicalIF":3.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem cell assisted low-intensity shockwave for erectile dysfunction treatment: Current perspective.","authors":"Datesh Daneshwar, Yemin Lee, Abid Nordin","doi":"10.1016/j.reth.2024.11.006","DOIUrl":"10.1016/j.reth.2024.11.006","url":null,"abstract":"<p><p>Stem cell therapy and low-intensity extracorporeal shockwave (LI-ECSW) are recognized as potential restorative therapies and have been used in the treatment of erectile dysfunction (ED). Stem cell therapy is well-known due to its attributed regenerative ability and thus can help to improve erectile function in patients with vasculogenic ED. Besides, current evidence also shows that LI-ECSW therapy can help stimulate cell recruitment and proliferation and promote angiogenesis and vascularization in the damaged tissue. Hence, due to the therapeutic and restorative effects of both therapies, the success of ED treatment can be elevated through a combination therapy between stem cell therapy and LI-ECSW. In this review, a detailed description and efficacy discussion of combination therapies between different types of stem cells and LI-ECSW therapy are described. Besides, other potential cell types to use together with LI-ECSW are also listed in this review. Thus, this review provides better insight on the efficacy of combination therapy for ED treatment.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1150-1158"},"PeriodicalIF":3.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight of the interrelationship and association mechanism between periodontitis and diabetes mellitus.","authors":"Yongqiang Yang, Xia Sun, Yucheng Yang, Yingchun Qie","doi":"10.1016/j.reth.2024.11.001","DOIUrl":"10.1016/j.reth.2024.11.001","url":null,"abstract":"<p><p>Periodontitis and diabetes mellitus are two prevalent chronic diseases that have been recognized to exhibit a bidirectional relationship. Individuals with diabetes are more susceptible to periodontitis, and conversely, periodontitis can exacerbate glycemic control in diabetic patients. The underlying mechanisms of this interrelationship involve complex pathways, including inflammatory responses, altered immune functions, and microbial dysbiosis. The mechanistic insights into the interrelationship between periodontitis and diabetes mellitus revolve around the role of inflammation as a common link between the two diseases. Inflammatory mediators such as cytokines, chemokines, and prostaglandins play a crucial role in the pathogenesis and progression of the diseases. The dysregulation of the immune response in diabetes can exacerbate the inflammatory response in periodontitis, leading to increased tissue destruction and bone resorption. The chronic inflammation in periodontitis can contribute to insulin resistance and impaired glycemic control in diabetic patients. Future directions in research aim to further elucidate the molecular mechanisms underlying the interrelationship between periodontitis and diabetes mellitus. Modulating the inflammatory response, restoring microbial balance, and improving glycemic control hold promise in managing both conditions simultaneously. Herein, we will provide an overview of the interrelationship of periodontitis and diabetes mellitus, and retrospect the underlying mechanisms, which may inspire investigators with further research directions.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1159-1167"},"PeriodicalIF":3.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived stem cells as a modern therapeutic approach for skin rejuvenation and hair regrowth.","authors":"Fatemeh Norouzi, Sanaz Aghajani, Nasim Vosoughi, Shiva Sharif, Kazem Ghahremanzadeh, Zeinab Mokhtari, Javad Verdi","doi":"10.1016/j.reth.2024.10.001","DOIUrl":"10.1016/j.reth.2024.10.001","url":null,"abstract":"<p><strong>Background: </strong>The skin covers the surface of the body and acts as the first defense barrier against environmental damage. Exposure of the skin to environmental physical and chemical factors such as mechanical injuries, UV rays, air pollution, chemicals, etc. Leads to numerous damages to skin cells such as fibroblasts, keratinocytes, melanocytes, etc. The harmful effects of environmental factors on skin cells could lead to various skin diseases, chronic wounds, wrinkles, and skin aging. Hair is an essential part of the body, serving multiple functions such as regulating body temperature and protecting against external factors like dust (through eyelashes and eyebrows). It also reflects an individual's personality. Therefore, the need for new treatment methods for skin diseases and lesions and at the same time preserving the youth, freshness, and beauty of the skin has been highly noticed by experts. Exosomes are nanovesicles derived from cells that contain various biological compounds such as lipids, proteins, nucleic acids, and carbohydrates. They are secreted by a variety of mammalian cells and even different plants. Exosomes are of great interest as a new therapeutic approach due to their stability, ability to be transported throughout the body, paracrine and endocrine effects, as well as the ability to carry various compounds and drugs to target cells.</p><p><strong>Aim: </strong>In this review, we have discussed the characteristics of exosomes, their cellular sources, and their therapeutic effects on wrinkles, skin aging, and rejuvenation and hair regrowth.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1124-1137"},"PeriodicalIF":3.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreasing electricity costs of clean room for cell products during non-operation.","authors":"Mitsuru Mizuno, Koki Abe, Takashi Kakimoto, Hisashi Hasebe, Ichiro Sekiya","doi":"10.1016/j.reth.2024.11.002","DOIUrl":"10.1016/j.reth.2024.11.002","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Cell processing facilities are susceptible to environmental bacteria and must maintain sterile environments to safeguard cell products. This process involves circulating air through high-efficiency particulate air (HEPA) filters, which incurs significant maintenance costs. While cost-reduction strategies have been explored in the semiconductor industry, validations specific to cell processing facilities remain unreported. This study aims to verify whether optimizing air-conditioning management in cell processing facilities can achieve energy savings by using particle counters to measure air quality during both non-operational and hypothetical operational conditions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The study assessed particle generation under varying air conditions to evaluate potential savings and the impact of reducing air-change rates. The air conditions were defined as follows: Condition 1 (C1) represented normal air conditions (100 %), followed by C2 (72.87 %), C3 (45.74 %), C4 (18.60 %), and C5 (0 %). The number of particles was evaluated across these conditions. Particle counters measured the quantity of particles during non-operational periods and during a 2-min walking motion. The time taken for particle levels to stabilize and become undetectable was also analyzed. Theoretical electricity cost savings were estimated for hypothetical operating and non-operating hours, with calculations adjusted for facilities ranging in size from small (100 m&lt;sup&gt;2&lt;/sup&gt;) to large (1000 m&lt;sup&gt;2&lt;/sup&gt;).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Results indicated that under air conditions C1, C2, C3, and C4, almost no particles were detected, whereas in C5, where air conditioning was halted, particle counts still remained below guideline values. Total particle counts at the four positions were significantly higher at both 0.5 and 5 μm under conditions C4 and C5 compared to other settings. The study also demonstrated that the rate of particle increase during operation varied by air-conditioning condition and position. Notably, reducing the air-change rate significantly enhanced energy savings, especially in larger facilities. For instance, annual electricity consumption in a large facility could potentially be reduced from approximately 31 million yen to approximately 9.6 million yen, yielding savings of approximately 20 million yen.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Even with a reduced air-change rate during non-operation, it was possible to maintain the cleanliness standards for each grade. The findings suggest that current operational practices are often excessive and that significant reductions in operating costs can be achieved by adjusting ventilation frequencies during non-operational periods. This study provides crucial insights for managing cell processing facilities facing challenges such as low production rates, the necessity of operating at full capacity due to on-demand autotransplantation, and high maintenance costs.&lt;/","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1117-1123"},"PeriodicalIF":3.4,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cells: Guardians of women's health.","authors":"Guanwen Gao, Li Li, Changling Li, Degao Liu, Yunfei Wang, Changzhong Li","doi":"10.1016/j.reth.2024.10.011","DOIUrl":"10.1016/j.reth.2024.10.011","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) have attracted more and more attention because of their multidirectional differentiation potential, immune regulatory abilities and self-renewal capacity. In recent years, their use has become prominent in the domains of regenerative medicine and tissue engineering. MSCs have shown promise in therapeutic studies for a variety of diseases and have become a new source of innovative solutions for the treatment of some obstetric and gynecological diseases. This review systematically presents the latest research on the use of MSCs in the treatment of obstetrics- and gynecology-related diseases. Specifically, this review encompasses the latest findings related to the role of MSCs in premature ovarian failure, polycystic ovary syndrome, ovarian cancer, fallopian tube-related diseases, uterine adhesions, endometriosis, cesarean scar defects, postmenopausal osteoporosis, and pelvic floor dysfunction. The shortcomings and challenges of the future use of MSCs in disease treatment are also discussed, with the intent to motivate improvements in MSC applications in clinical therapy. It is believed that with further research, MSCs will play a more important role in the treatment of obstetrics- and gynecology-related diseases.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1087-1098"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles originating from the mechanical microenvironment in the pathogenesis and applications for cardiovascular diseases.","authors":"Yu Zeng, Xiaodong Cui, Hong Li, Yanhui Wang, Min Cheng, Xiaoyun Zhang","doi":"10.1016/j.reth.2024.10.012","DOIUrl":"10.1016/j.reth.2024.10.012","url":null,"abstract":"<p><p>The mechanical microenvironment plays a crucial regulatory role in the growth and development of cells. Mechanical stimuli, including shear, tensile, compression, and extracellular matrix forces, significantly influence cell adhesion, migration, proliferation, differentiation, and various other cellular functions. Extracellular vesicles (EVs) are involved in numerous physiological and pathological processes, with their occurrence and secretion being strictly regulated by the mechanical microenvironment. Recent studies have confirmed that alterations in the mechanical microenvironment are present in cardiovascular diseases, and the components of EVs can respond to changes in mechanical signals, thereby impacting the progression of these diseases. Additionally, engineered EVs, created by leveraging mechanical microenvironments, can serve as natural drug-delivery vehicles for treating and managing specific diseases. This article systematically reviews the regulatory mechanisms through which the mechanical microenvironment influences EVs and summarizes the role and advancements of EVs derived from this environment in the context of cardiovascular diseases.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1069-1077"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1.","authors":"Yin-Tzu Chen, Tai-Horng Young, Yu-Hsin Wang, Chih-Hsuan Huang, Yu-Yun Gao, Tsung-Wei Huang","doi":"10.1016/j.reth.2024.10.014","DOIUrl":"10.1016/j.reth.2024.10.014","url":null,"abstract":"<p><strong>Introduction: </strong>Sensorineural olfactory dysfunction significantly impairs the life quality of patients but without effective treatments to date. Orexin is a neurotrophic factor activates neuronal network activity. However, it is still unknown whether orexin can promote differentiation in human olfactory sensory neurons (OSNs). This study seeks to explore the impact of orexin on the differentiation of human olfactory neuroepithelial cells (HONCs).</p><p><strong>Methods: </strong>The primary olfactory epithelium cells were cultured with or without orexin-A. The neural maturation-related and functional proteins were analyzed through immunofluorescence staining and Western blot. The function of HONCs were evaluated through the synaptic vesicle recycling assay.</p><p><strong>Results: </strong>The results showed that HONCs in the orexin-A group expressed higher levels of stage-specific markers, including achaete-scute homolog 1, βIII-tubulin, and olfactory marker protein. Additionally, more components of signaling transduction pathways compared to the control group. The orexin-A-mediated differentiation of OSN effect can be nullified with dual orexin receptor antagonist suvorexant and the selective orexin receptor type 1 antagonist SB674042, instead of selective orexin receptor type 2 antagonist TCS-OX2-29.</p><p><strong>Conclusions: </strong>Orexin-A elevates the expression of protein markers in human olfactory neuronal progenitor cells to stimulate the differentiation of OSN and enhances the formation of components of the olfactory-specific signaling transduction pathway via orexin receptor type 1.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1058-1068"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds.","authors":"Yu Yamazaki, Kaoruko Kikuchi, Yoko Yamada, Sakurako Neo, Suguru Nitta, Hirotaka Igarashi, Akihide Kamiya, Masaharu Hisasue","doi":"10.1016/j.reth.2024.09.003","DOIUrl":"10.1016/j.reth.2024.09.003","url":null,"abstract":"<p><strong>Introduction: </strong>Exploring techniques for differentiating and culturing canine hepatocytes serves as a means to establish systems for liver transplantation and drug metabolism testing. However, establishing consistent methods for culturing stable hepatocytes remains a challenge. Recently, several investigations have shown the reprogramming of mature hepatocytes into hepatic progenitor cells by applying specific small molecule compounds, including Y-27632, (a ROCK inhibitor), A-83-01 (a TGFβ inhibitor), and CHIR99021 (a GSK3 inhibitor) (termed YAC) in rat, mouse, and humans, respectively. However, reports or evidence of successful reprogramming using these small-molecule compounds in dogs are absent. This study aimed to induce the differentiation of mature canine hepatocytes into progenitor cells.</p><p><strong>Methods: </strong>Cryopreserved canine hepatocytes (cHep) were cultured for 14 d in a YAC-supplemented hepatocyte growth medium. Subsequently, an assessment was conducted involving morphological observations, quantitative real-time polymerase chain reaction (qRT-PCR), and immunocytochemistry.</p><p><strong>Results: </strong>Notably, cryopreserved cHep cells emerged and exhibited ongoing proliferation and concurrently developed colonies within the YAC-enriched culture. These observations indicated that the mature hepatocytes reprogrammed into hepatic progenitor cells. Moreover, qRT-PCR analysis revealed a notable enhancement in gene expression levels. Specifically, the genes encoding α-fetoprotein (AFP), epithelial cell adhesion molecule (EpCAM), Cytokeratin 19 (CK19) and SRY-box9 (Sox9) displayed approximately 12-, 2.2-, 517- and 2.9- increases in hepatic progenitor cells, respectively, on day 14 as compared to their state before induction of differentiation. Hepatocyte-related protein expression of AFP, EPCAM, SOX9 and CK19 was confirmed via immunocytochemistry on day 21. In contrast, ALB and MRP2, which are highly expressed in mature hepatocytes, were decreased compared to those before YAC addition, which is consistent with the characteristics of undifferentiated hepatocytes.</p><p><strong>Conclusions: </strong>Herein, we effectively promoted the reprogramming of cryopreserved cHep cells into hepatic progenitor cells using three small-molecule compounds. The mRNA and protein expression analyses demonstrated increased levels of hepatic progenitor cells-specific markers, whereas markers related to mature hepatocytes decreased, suggesting that reprogramming cryopreserved cHep cells to hepatic progenitor cells was achieved using YAC. Therefore, cultivating liver progenitor cells holds the potential to offer valuable insights into the development of artificial livers for drug discovery research and transplantation therapy aimed at addressing liver diseases in dogs.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1078-1086"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scientometric and visualization analysis of 3D printing scaffolds for vascularized bone tissue engineering over the last decade.","authors":"Siyang Cao, Yihao Wei, Yaohang Yue, Deli Wang, Ao Xiong, Jun Yang, Hui Zeng","doi":"10.1016/j.reth.2024.10.013","DOIUrl":"10.1016/j.reth.2024.10.013","url":null,"abstract":"<p><p>The introduction of three-dimensional (3D) printing scaffolds has emerged as an effective approach to achieving satisfactory revascularization for bone tissue engineering (BTE). However, there is a notable absence of analytical and descriptive investigations concerning the trajectory, essential research directions, current research scenario, pivotal investigative focuses, and forthcoming perspectives. Hence, the objective of this research is to offer a thorough overview of the advancements achieved in 3D printing structures for vascularized BTE within the last 10 years. Information extracted from the Web of Science repository spans from January 1, 2014, to April 1, 2024. Utilizing advanced analytical instruments, we conducted comprehensive scientometric and visual analyses. The findings underscore the predominant influence of China, representing 59.62 % of the overall publications and playing a pivotal role in shaping research within this field. Notable productivity was evident at various institutions, including Shanghai Jiao Tong University, Chinese Academy of Sciences, and Sichuan University. Wang Jinwu and Wu Chengtie stand out as the most prolific contributors in this domain. The highest number of publications in this area was contributed by the journal <i>Advanced Healthcare Materials</i>. In this study, osteogenesis imperfecta, osteosarcoma, fractures, osteonecrosis, and cartilage diseases were identified as the most significant disorders investigated in this research area. By providing a comprehensive scientometric assessment, this study benefits both experienced researchers and newcomers alike, offering prompt access to essential information and fostering the extraction of innovative concepts within this specific field.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1099-1116"},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}