Regenerative Therapy最新文献

{"title":"The effects of local delivery of laurus nobilis extract and adipose derived stem cells via electrospun gelatin scaffold on spinal cord injury inflammatoradscy response and its regeneration","authors":"Chong Zhang ,&nbsp;Jin Zhang ,&nbsp;Daotao Xie ,&nbsp;Gang Guo ,&nbsp;Saman Jalili","doi":"10.1016/j.reth.2024.09.008","DOIUrl":"10.1016/j.reth.2024.09.008","url":null,"abstract":"<div><div>When subjected to injury, the spinal cord's inherent complexity poses significant challenges for effective healing. In this study, gelatin nanofibers loaded with Laurus nobilis extract were developed to serve as a delivery system for adipose-derived stem cells (ADSCs), aiming to explore its potential immunomodulatory effects in a rat model of spinal cord injury. Through a series of in vitro assessments including scanning electron microscopy imaging, cell viability, anti-inflammatory, cell adhesion, biodegradation, and hemocompatibility assays, the characteristics of the delivery system were thoroughly evaluated. The in vitro studies revealed both the biocompatibility of the scaffolds and their notable anti-inflammatory properties, laying the groundwork for further investigation. Subsequent in vivo experiments demonstrated that rats treated with Laurus nobilis extract and ADSCs loaded scaffolds exhibited heightened functional recovery (BBB score of 14.66 ± 1.52 % and hot plate latency time of 8.33 0.26 s) and histological restoration at the 8-week mark post-injury. Notably, ELISA assay results revealed a significant reduction in tissue expression levels of key pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, suggesting a pronounced immunomodulatory effect of the Laurus nobilis extract-loaded scaffolds. The findings underscore the potential of this novel delivery system to improve clinical outcomes in spinal cord injury by enhancing functional recovery and reducing inflammation. This approach could lead to the development of new, natural-based therapeutic strategies for spinal cord injury, with potential extensions to other inflammatory or degenerative conditions. Future research should focus on optimizing this strategy in larger animal models and eventually translating these findings into human clinical trials.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 879-888"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wound fluid sampling methods and analysis of cytokine mRNA expression in ulcers from patients with diabetes mellitus","authors":"Beatriz Barbieri ,&nbsp;Amanda Silva ,&nbsp;Joseane Morari ,&nbsp;Flavia C. Zanchetta ,&nbsp;Bianca Oliveira ,&nbsp;Alexis Trott ,&nbsp;Eliana P. Araújo ,&nbsp;Geraldo Paula ,&nbsp;Beatriz Guitton Renaud Baptista de Oliveira ,&nbsp;Bruna Maiara Ferreira Barreto Pires ,&nbsp;Maria Helena Melo Lima","doi":"10.1016/j.reth.2024.06.016","DOIUrl":"https://doi.org/10.1016/j.reth.2024.06.016","url":null,"abstract":"<div><p>The development of diabetic foot ulcers is a common and severe complication of diabetes that can significantly affect quality of life. The physiological healing cascade does not progress tissue repair in diabetic foot ulcerations in a timely manner. Serum markers from foot ulcers have been used to characterize the healing process of the diabetic foot using various collection techniques. This study aimed to compare the use of cervical brushes and the Levine technique to collect wound fluid from foot ulcers of people with diabetes in order to determine the presence of cytokines. The collected material was used for gene expression analysis of macrophage/monocyte-associated cytokines IL1-β, IL-6, TNF-α, regulatory cytokine IL-10 and growth factor TGFβ, via quantitative polymerase chain reaction (qPCR). Both collection methods produced sufficient amounts of RNA, but significantly more RNA was collected using a cervical brush (brush 224.82 ng/μL vs. Levine 80.90 ng/μL p = 0.0001). Significantly higher levels of expression of the following cytokine genes were detected in samples collected using a cervical brush: IL1-β (p = 0.0001), IL-6 (p = 0.0106), IL-10 (p = 0.0277) and TGFβ (p = 0.0002). Understanding why some wounds are difficult to heal is important for developing more effective treatments, and biomarkers may be useful for predicting the healing trajectory. These results demonstrate that it is possible to collect material from the wound bed for RT-qPCR analysis, and the cervical brush proved to be a simple and rapid method for monitoring cytokine gene expression.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 425-431"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001238/pdfft?md5=67f8b3933f274a1ccef11588e4a18e86&pid=1-s2.0-S2352320424001238-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141583233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse events related to platelet-rich plasma therapy and future issues to be resolved","authors":"Anna Arita,&nbsp;Morikuni Tobita","doi":"10.1016/j.reth.2024.07.004","DOIUrl":"10.1016/j.reth.2024.07.004","url":null,"abstract":"<div><p>Platelet-rich plasma (PRP) is the portion of plasma with a platelet concentration above baseline that is recovered through centrifugation of autologous blood. PRP therapy is currently used for wound healing and pain relief in diverse medical fields. Although there have been recent reports of adverse events (AEs) possibly related to PRP treatment, the safety profile of PRP treatment remains unclear. Therefore, this review discusses the risks inherent in PRP therapy and the current issues by surveying reports on AEs associated with PRP treatment within different fields.</p><p>PubMed was searched for research articles referring to AEs associated with PRP therapy from inception to January 2024. Literature survey revealed that PRP therapy may involve several AEs, including postoperative infections, blindness, inflammation, allergic reactions, and nodule development. The most commonly reported AE was postoperative infections. Since PRP therapy generally proceeds in the process of blood collection, manufacturing, and administration to patients, it is conjectured that PRP may have been contaminated with microorganisms at some point in this series of processes, leading to bacterial infection. Additionally, because PRP cannot be sterilized like pharmaceuticals, it is important to prevent microbial contamination during each PRP treatment process. However, the specific process that involves the risk of microbial contamination remains unclear. To take measures to prevent microbial contamination of PRP, it may be necessary to elucidate the risk factors for microbial contamination during PRP treatment. It may be important to elucidate the effectiveness and risks of PRP therapy as well as to establish a follow-up system after PRP treatment. Currently, most reports of AEs related to PRP therapy are case reports; therefore, the accumulation of high-quality evidence and detailed verification are necessary to determine the causal relationship between PRP therapy and each AE.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 496-501"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001329/pdfft?md5=44d9ed4206195710c671d31f7ed990c6&pid=1-s2.0-S2352320424001329-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141732304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of GDF-5 for enhancing tendon regenerative healing","authors":"Hanyue Li ,&nbsp;Yini Li ,&nbsp;Linmei Xiang ,&nbsp;Shengyu Luo ,&nbsp;Yan Zhang ,&nbsp;Sen Li","doi":"10.1016/j.reth.2024.03.029","DOIUrl":"https://doi.org/10.1016/j.reth.2024.03.029","url":null,"abstract":"<div><p>Tendon injury is a common disorder of the musculoskeletal system, with a higher possibility of occurrence in elderly individuals and athletes. After a tendon injury, the tendon suffers from inadequate and slow healing, resulting in the formation of fibrotic scar tissue, ending up with inferior functional properties. Therapeutic strategies involving the application of growth factors have been advocated to promote tendon healing. Growth and differentiation-5 (GDF-5) represents one such factor that has shown promising effect on tendon healing in animal models and in vitro cultures. Although promising, these studies are limited as the molecular mechanisms by which GDF-5 exerts its effect remain incompletely understood. Starting from broadly introducing essential elements of current understanding about GDF-5, the present review aims to define the effect of GDF-5 and its possible mechanisms of action in tendon healing. Nevertheless, we still need more in vivo studies to explore dosage, application time and delivery strategy of GDF-5, so as to pave the way for future clinical translation.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 290-298"},"PeriodicalIF":4.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424000610/pdfft?md5=96982c681046d4dd17ae977453b1a5cc&pid=1-s2.0-S2352320424000610-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141429597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory systems and requirements for clinical trials of AAV-based gene therapies – Perspectives from six Asian countries or regions: Report from the 6th Asia Partnership Conference of Regenerative Medicine – April 20, 2023","authors":"Hirokuni Mizoguchi ,&nbsp;Alex J. Zhang ,&nbsp;Pawan Kumar Gupta ,&nbsp;Masato Komuro ,&nbsp;Wong Kum Cheun ,&nbsp;Chuan Wen Chiu ,&nbsp;Bryan Choi","doi":"10.1016/j.reth.2024.06.005","DOIUrl":"https://doi.org/10.1016/j.reth.2024.06.005","url":null,"abstract":"<div><p>Gene therapies, which include viral-vector gene delivery, genome editing, and genetically modified cell therapy, are innovative treatments with the potential to address the underlying genetic causes of disorders and to provide life-changing value in terms of curing disease. Although adeno-associated virus (AAV)-based gene therapy is one of the most advanced types of gene therapy, far fewer AAV-based gene therapy studies have been conducted in Asia than in North America and Europe. The 6th Asia Partnership Conference of Regenerative Medicine (APACRM) was held on April 20, 2023 in Tokyo, Japan. APACRM Working Group 3 comprehensively analyzed the regulatory processes that occur prior to the initiation of clinical trials as well as the regulatory requirements for AAV-based gene therapies for six Asian countries or regions (China, India, Japan, Singapore, South Korea, and Taiwan). In this article, we report the outcomes of this conference, summarizing the regulatory requirements for initiating clinical trials for AAV-based gene therapies in terms of the laws, regulations, and guidelines for gene therapy; consultations or reviews required by the health authorities; points to consider for scientific reviews by the health authorities; and specific challenges to address when developing gene therapy products in these locations. Finally, we present several policy recommendations, including simplifying the regulatory review system for multiple scientific review areas; simplifying the regulatory consultation system; and providing training programs and regulatory guidance to support the advancement of gene therapy development in Asia.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 334-345"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001111/pdfft?md5=99d702d482eeea618ee7a849e7ba6ad7&pid=1-s2.0-S2352320424001111-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141483234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokines released from human adipose tissue-derived stem cells by bFGF stimulation: Effects of IL-8 and CXCL-1 on wound healing","authors":"Chihiro Matsui ,&nbsp;Hiroshi Koide ,&nbsp;Tomomi Ikeda ,&nbsp;Takako Ikegami ,&nbsp;Takumi Yamamoto ,&nbsp;Joseph M. Escandón ,&nbsp;Arbab Mohammad ,&nbsp;Tomoyuki Ito ,&nbsp;Hiroshi Mizuno","doi":"10.1016/j.reth.2024.06.008","DOIUrl":"https://doi.org/10.1016/j.reth.2024.06.008","url":null,"abstract":"<div><h3>Objectives</h3><p>Adipose-derived stem cells (ADSCs) are widely used in wound care because they release a variety of cytokines. However, the molecular mechanism of paracrine action remains unclear. It has been reported that basic fibroblast growth factor (bFGF) enhances the therapeutic potential of ADSCs. In this study, we searched for cytokines whose release from ADSCs is enhanced by bFGF stimulation.</p></div><div><h3>Results</h3><p>Quantitative RT-PCR and ELISA analyses revealed that bFGF upregulates CXCL-1 and IL-8 mRNA synthesis and secretion from ADSCs. Both cytokines showed the ability to promote important processes for wound healing, including tube formation of vascular and lymphatic endothelial cells and cell migration of fibroblasts <em>in vitro</em>.</p></div><div><h3>Conclusions</h3><p>These results suggest that bFGF stimulation increases the secretion of CXCL-1 and IL-8 from ADSCs and that these cytokines may promote angiogenesis, lymphangiogenesis, and cell migration, leading to enhanced efficiency of wound healing.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 401-406"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001160/pdfft?md5=d150b7acdc5fe3dbd4f1cd06101a21e1&pid=1-s2.0-S2352320424001160-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of insulin-like growth factor 1 receptor-knockout pigs as a potential system for interspecies organogenesis","authors":"Masaki Nagaya ,&nbsp;Ayuko Uchikura ,&nbsp;Kazuaki Nakano ,&nbsp;Masahito Watanabe ,&nbsp;Hitomi Matsunari ,&nbsp;Kazuhiro Umeyama ,&nbsp;Naoaki Mizuno ,&nbsp;Toshiya Nishimura ,&nbsp;Hiromitsu Nakauchi ,&nbsp;Hiroshi Nagashima","doi":"10.1016/j.reth.2024.08.025","DOIUrl":"10.1016/j.reth.2024.08.025","url":null,"abstract":"<div><h3>Background</h3><p>To overcome organ shortage during transplantation, interspecies organ generation via blastocyst complementation has been proposed, although not yet in evolutionarily distant species. To establish high levels of chimerism, low chimerism is required early in development, followed by high chimerism, to effectively complement the organ niche. Very few human cells are expected to contribute to chimerism in heterologous animals. Previous studies had demonstrated increased donor chimerism in both intra- and interspecies chimeras in rodents, using <em>insulin-like growth factor 1 receptor</em> (<em>Igf1r</em>) knockout (KO) mice; deletion of the <em>Igf1r</em> gene in the mouse host embryo created a cell-competitive niche. The current study aimed to generate <em>IGF1R–</em>KO pigs and evaluate whether they have the same phenotype as <em>Igf1r</em>-KO mice.</p></div><div><h3>Methods</h3><p>To generate <em>IGF1R–</em>KO pigs, genome-editing molecules were injected into the cytoplasm of pig zygotes. The fetuses were evaluated at 104 days of gestation.</p></div><div><h3>Results</h3><p><em>IGF1R</em>–KO pigs were generated successfully. Their phenotypes were almost identical to those of <em>Igf1r</em>-KO mice, including small lungs and enlarged endodermal organs in fetuses, and they were highly reproducible.</p></div><div><h3>Conclusions</h3><p>Pigs may allow the generation of organs using blastocyst complementation with developmentally-compatible xenogeneic pluripotent stem cells over a large evolutionary distance.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 783-791"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001597/pdfft?md5=e56454f3d95607e8e040277970a84e44&pid=1-s2.0-S2352320424001597-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from epidermal growth factor-like domain protein 6-preconditioned mesenchymal stem cells for diabetic wound healing","authors":"Chen Gong ,&nbsp;Chengde Xia ,&nbsp;Linbo Liu","doi":"10.1016/j.reth.2024.09.009","DOIUrl":"10.1016/j.reth.2024.09.009","url":null,"abstract":"<div><div>Diabetic wounds are difficult to repair effectively in the clinic. Tissue engineering based on mesenchymal stem cells (MSCs) showed great therapeutic potential in wound healing. MSCs-derived exosome could reproduce the effect of MSCs by transferring the bioactive substance to the recipient cells. The biological function of exosomes was determined by the state of the derived MSCs. In this study, we cultured hUC-MSCs with EGFL6 and isolated EGFL6-preconditioned exosomes (EGF-Exos), and then investigated the effect of EGF-Exos on wound healing. The results revealed that EGF-Exos promoted the proliferation and migration of HUVECs, had the anti-inflammtory function and improved angiogenesis. Moreover, we fabricated Gelama hydrogel to load EGF-Exos to repair diabetic wounds. <em>In vivo</em> results showed that EGF-Exos contributed to the repair of diabetic wound and provided valuable data for understanding the role of EGF-Exos in diabetic wound healing.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 932-940"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skeletal muscle injury treatment using the Silk Elastin® injection in a rat model","authors":"Kyohei Nakata ,&nbsp;Masakazu Ishikawa ,&nbsp;Naosuke Kamei ,&nbsp;Shigeru Miyaki ,&nbsp;Nobuo Adachi ,&nbsp;Keiichiro Inoue ,&nbsp;Shingo Kawabata","doi":"10.1016/j.reth.2024.05.012","DOIUrl":"https://doi.org/10.1016/j.reth.2024.05.012","url":null,"abstract":"<div><h3>Background</h3><p>Skeletal muscle injury (SMI) is often treated conservatively, although it can lead to scar tissue formation, which impedes muscle function and increases muscle re-injury risk. However, effective interventions for SMIs are yet to be established.</p></div><div><h3>Hypothesis</h3><p>The administration of Silk Elastin® (SE), a novel artificial protein, to the SMI site can suppress scar formation and promote tissue repair.</p></div><div><h3>Study design</h3><p>A controlled laboratory study.</p></div><div><h3>Methods</h3><p><em>In vitro</em>: Fibroblast migration ability was assessed using a scratch assay. SE solution was added to the culture medium, and the fibroblast migration ability was compared across different concentrations. <em>In vivo</em>: An SMI model was established with Sprague–Dawley rats, which were assigned to three groups based on the material injected to the SMI site: SE gel (SE group; n = 8), atelocollagen gel (Atelo group; n = 8), and phosphate buffer saline (PBS group; n = 8). Histological evaluations were performed at weeks 1 and 4 following the SMI induction. In the 1-week model, we detected the expression of transforming growth factor (TGF)-β1 in the stroma using immunohistological evaluation and real-time polymerase chain reaction analysis. In the 4-week model, we measured tibialis anterior muscle strength upon peroneal nerve stimulation as a functional assessment.</p></div><div><h3>Results</h3><p><em>In vitro</em>: The fibroblast migration ability was suppressed by SE added at a concentration of 10⁴ μg/mL in the culture medium. <em>In vivo</em>: In the 1-week model, the SE group exhibited significantly lower TGFβ −1 expression than the PBS group. In the 4-week model, the SE group had a significantly larger regenerated muscle fiber diameter and smaller scar formation area ratio than the other two groups. Moreover, the SE group was superior to the other two groups in terms of regenerative muscle strength.</p></div><div><h3>Conclusion</h3><p>Injection of SE gel to the SMI site may inhibit tissue scarring by reducing excessive fibroblast migration, thereby enhancing tissue repair.</p></div><div><h3>Clinical relevance</h3><p>The findings of this study may contribute to the development of an early intervention method for SMIs.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 180-187"},"PeriodicalIF":4.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424000993/pdfft?md5=7ba05ddf601d47aa939e6adf1e03de7a&pid=1-s2.0-S2352320424000993-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical application of a hyaluronic acid-based hydrogel integrated with secretome of human mesenchymal stromal cells for diabetic ulcer repair","authors":"Fabio Salvatore Palumbo ,&nbsp;Matteo Calligaris ,&nbsp;Laura Calzà ,&nbsp;Calogero Fiorica ,&nbsp;Vito Antonio Baldassarro ,&nbsp;Anna Paola Carreca ,&nbsp;Luca Lorenzini ,&nbsp;Alessandro Giuliani ,&nbsp;Claudia Carcione ,&nbsp;Nicola Cuscino ,&nbsp;Giovanna Pitarresi ,&nbsp;Simone Dario Scilabra ,&nbsp;Pier Giulio Conaldi ,&nbsp;Cinzia Maria Chinnici","doi":"10.1016/j.reth.2024.07.008","DOIUrl":"10.1016/j.reth.2024.07.008","url":null,"abstract":"<div><p>This preclinical proof-of-concept study aimed to evaluate the effectiveness of secretome therapy in diabetic mice with pressure ulcers. We utilized a custom-made hyaluronic acid (HA)-based porous sponge, which was rehydrated either with normal culture medium or secretome derived from human mesenchymal stromal cells (MSCs) to achieve a hydrogel consistency. Following application onto skin ulcers, both the hydrogel-only and the hydrogel + secretome combination accelerated wound closure compared to the vehicle group. Notably, the presence of secretome significantly enhanced the healing effect of the hydrogel, as evidenced by a thicker epidermis and increased revascularization of the healed area compared to the vehicle group. Notably, molecular analysis of healed skin revealed significant downregulation of genes involved in delayed wound healing and abnormal inflammatory response in ulcers treated with the hydrogel + secretome combination, compared to those treated with the hydrogel only. Additionally, we found no significant differences in therapeutic outcomes when comparing the use of secretome from fetal dermal MSCs to that from umbilical cord MSCs. This observation is supported by the proteomic profile of the two secretomes, which suggests a shared molecular signature responsible of the observed therapeutic effects.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 520-532"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001366/pdfft?md5=b100b3562cc7d084e448cbc6323d30e2&pid=1-s2.0-S2352320424001366-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141949970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}