Regenerative Therapy最新文献

{"title":"Meta-analysis highlight the therapeutic potential of stem cells for premature ovarian failure","authors":"Amna Umer ,&nbsp;Khalil Ahmad ,&nbsp;Nasar Khan ,&nbsp;David Lawrence Greene ,&nbsp;Sabiha Shamim ,&nbsp;Umm E. Habiba","doi":"10.1016/j.reth.2024.07.001","DOIUrl":"10.1016/j.reth.2024.07.001","url":null,"abstract":"<div><p>Stem cell (SC) transplantation has shown potential as a therapeutic approach for premature ovarian failure (POF). Despite this, no quantitative analysis has been conducted on the efficacy of SC therapy for POF in humans. To address this gap, the present study conducted a meta-analysis to evaluate the effectiveness of the transplantation of SC in improving ovarian function among POF patients. A systematic review in this regard by searching PubMed, ScienceDirect, clinicalTrial.gov, and Cochrane's library databases was conducted to identify relevant studies, while associated reviews were also considered. The extracted data included parameters such as estradiol (E2), follicle-stimulating hormone (FSH), follicle count (FC), ovarian weight (OW), number of pregnancies, and live birth. As per the combined effect taking the last follow-up time, the level of FSH and AMH for the SC group was lower than these were at the baseline as (SMD: 1.58, 95% CI: 0.76 to 3.92, P-value: 0.185 &gt; 0.05, I²: 94.03%) and (SMD: 1.34, 95% CI: 0.77 to 1.92, P-value: 0.001 &lt; 0.05, I²: 0%) respectively. While the means of E2 and OW for the SC group was higher than these were at the baseline as (SMD: −0.47, 95% CI: −0.73 to −0.21, P-value: 0.001 &lt; 0.01, I²: 38.23%) and (SMD: −1.18, 95% CI: −2.62 to 0.26, P-value: 0.108 &gt; 0.05, I²: 76.68%) respectively. The overall effect size measured with proportion of pregnancy and live birth at a 5% level of significance expected SC transplantation results were as (combined proportion: 0.09, 95% CI: 0.03 to 0.15, P-value: 0.002 &lt; 0.05, I²: 46.29%) and (SMD: 0.09, 95% CI: 0.03 to 0.15, P-value: 0.003 &lt; 0.05, I²: 1.76%) respectively. Based on the fixed-effects model, the estimated average log odds ratio of Follicles count was 1.0234 (95% CI: 0.1252 to 1.9216). Therefore, the average outcome differed significantly from zero (P-value: 0.0255 &lt; 0.05) due to SC transplantation. These results suggest that using SCs to restore ovarian function may be viable for treating POF. However, larger and better-quality investigations would need to be conducted in the future due to the heterogeneity of the examined studies.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 478-488"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001299/pdfft?md5=6df36b12b0334769dbf8674133764709&pid=1-s2.0-S2352320424001299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141638252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing renal research: The future of kidney-on-a-chip in biotechnology","authors":"Yusuke Nishimura","doi":"10.1016/j.reth.2024.06.006","DOIUrl":"https://doi.org/10.1016/j.reth.2024.06.006","url":null,"abstract":"<div><p>In vitro models of kidneys have limited effectiveness owing to the complex structure and functions of the kidney when compared with other organs. Therefore many renal function evaluations are currently being carried out through animal experiments. In contrast, efforts are being made to apply biomimetic systems, such as organ-on-a-chip, which is based on microfluidic device technology, to serve as an in vitro model for the kidney. These systems aimed to recreate a physiological cultivation environment. This review has provided an overview of organ-on-a-chip research focused on glomeruli and tubules as in vitro models for the kidney and discusses future prospects.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 275-280"},"PeriodicalIF":4.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001135/pdfft?md5=8279c510ca98bb1c2928617961ccd1d4&pid=1-s2.0-S2352320424001135-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141423552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicle-liposome hybrids via membrane fusion using cell-penetrating peptide-conjugated lipids","authors":"Yuya Sato ,&nbsp;Weixu Zhang ,&nbsp;Teruhiko Baba ,&nbsp;Ung-il Chung ,&nbsp;Yuji Teramura","doi":"10.1016/j.reth.2024.07.006","DOIUrl":"10.1016/j.reth.2024.07.006","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are natural carriers for intercellular communication within the human body. Mimicking and utilizing EVs by combining them with artificial nanocarriers such as liposomes for drug delivery has garnered considerable attention. However, current technologies for manipulating EVs to facilitate their fusion with liposomes are limited; the existing technique of polyethylene glycol (PEG)-induced fusion is highly inefficient for fusion. In our previous study, we demonstrated that membrane fusion could be induced by Tat peptide (YGRKKRRQRRR)-conjugated poly(ethylene glycol)-phospholipids (Tat-PEG-lipids), in which the Tat peptide and lipid domain facilitate membrane attachment and subsequent fusion between cells and liposomes. This approach is promising for forming EV and liposomal hybrids. In this study, we aim to fuse EVs and liposomes using Tat-PEG-lipids. We isolated and characterized EVs derived from HEK293T cell culture medium and treated a mixture of EVs and liposomes composed of 1,2-dipalmitoyl-<em>sn</em>-glycero-3-phosphocholine and cholesterol (1:1, molar ratio), with Tat-PEG-lipids with different lipid chain lengths. Here, we used nonanoyl (C9), dodecanoyl (C12), and myristoyl (C14) groups as lipid anchors with 5 kDa PEG chains. Dynamic light scattering analysis revealed a large increase in the apparent size of mixture of EVs and liposomes by adding Tat-PEG-lipids (especially C14, C12, followed by C9). Fluorescence resonance energy transfer, confocal laser scanning microscopy, and transmission electron microscopy, used to analyze the reaction process, revealed that the membrane fusion occurred between EVs and liposomes but not their aggregates. The short lipid domain of Tat-PEG-lipids effectively induced membrane fusion and the formation of hybrid EVs and liposomes. Thus, Tat-PEG-lipids (C9 and C12) could be promising candidates for inducing membrane fusion to fabricate EV-liposome hybrids.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 533-540"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001342/pdfft?md5=cc3217ff798fba428b75a85d6ea67dbd&pid=1-s2.0-S2352320424001342-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141950279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series","authors":"Yasuhiro Maruya ,&nbsp;Yuko Akazawa ,&nbsp;Kiyuu Norimatsu ,&nbsp;Yerkezhan Sailaubekova ,&nbsp;Nazigul Zhumagazhiyeva ,&nbsp;Shinichiro Kobayashi ,&nbsp;Miki Higashi ,&nbsp;Keiichi Hashiguchi ,&nbsp;Naoyuki Yamaguchi ,&nbsp;Masahiro Nakashima ,&nbsp;Kazuhiko Nakao ,&nbsp;Kengo Kanetaka ,&nbsp;Susumu Eguchi","doi":"10.1016/j.reth.2024.08.007","DOIUrl":"10.1016/j.reth.2024.08.007","url":null,"abstract":"<div><p>Autologous oral mucosal epithelial cell sheet (AOMECS) transplantation has recently been applied in human patients to prevent postprocedural stenosis following endoscopic submucosal dissection (ESD) for esophageal squamous cell carcinoma. However, the long-term safety of AOMECS transplantation remains unclear. We evaluated the long-term outcomes of 10 patients who participated in a clinical trial of AOMECS transplantation after esophageal ESD. Additionally, we assessed the local DNA damage response in the esophageal epithelium using p53 binding protein 1 (53BP1) immunofluorescence in post-AOMECS biopsy specimens. The median follow-up period was 118.5 months (range: 46–130 months). Two patients developed primary esophageal cancer near the AOMECS site and successfully underwent additional ESD. One patient developed lymph node metastasis and underwent chemotherapy. None of the patients died from the original disease, although one patient died from unrelated causes. The rate of abnormal 53BP1 nuclear foci, indicative of increased genome instability, increased with the progression of neoplasia in patients post AOMECS. Our case series suggests that AOMECS transplantation provides an acceptable long-term prognosis and 53BP1 foci may serve as a useful marker for assessing DNA instability in the post-AOMECS esophageal epithelium.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 557-563"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001433/pdfft?md5=b5ec71b720f5b50a171febc18c3b633d&pid=1-s2.0-S2352320424001433-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical stimulation induced pre-vascularization of engineered dental pulp tissue","authors":"Ying-tong Wang ,&nbsp;Jia-ying Zhou ,&nbsp;Kai Chen ,&nbsp;Xiao Yu ,&nbsp;Zhi-yong Dong ,&nbsp;Yu-shan Liu ,&nbsp;Xiao-ting Meng","doi":"10.1016/j.reth.2024.06.014","DOIUrl":"https://doi.org/10.1016/j.reth.2024.06.014","url":null,"abstract":"<div><p>Vascularization is a key step to achieve pulp tissue regeneration and <em>in vitro</em> pre-vascularized dental pulp tissue could be applied as a graft substitute for dental pulp tissue repair. In this study, human dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (hUVECs) were co-cultured in 3D Matrigel and 150 mV/mm electric fields (EFs) were used to promote the construction of pre-vascularized dental pulp tissue. After optimizing co-cultured ratio of two cell types, immunofluorescence staining, and live/dead detection were used to investigate the effect of EFs on cell survival, differentiation and vessel formation in 3D engineered dental pulp tissue. RNA sequencing was used to investigate the potential molecular mechanisms by which EF regulates vessel formation in 3D engineered dental pulp tissue. Here we identified that EF-induced pre-vascularized engineered dental pulp tissue not only had odontoblasts, but also had a rich vascular network, and smooth muscle-like cells appeared around the blood vessels. The GO enrichment analysis showed that these genes were significantly enriched in regulation of angiogenesis, cell migration and motility. The most significant term of the KEGG pathway analysis were NOTCH signaling pathway and Calcium signaling pathway etc. The PPI network revealed that NOTCH1 and IL-6 were central hub genes. Our study indicated that EFs significantly promoted the maturation and stable of blood vessel in 3D engineered pulp tissue and provided an experimental basis for the application of EF in dental pulp angiogenesis and regeneration.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 354-365"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001214/pdfft?md5=8d2fb8b43749ed97894a88f8dcc0f5a2&pid=1-s2.0-S2352320424001214-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141483236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subpial transplantation of adipose-derived stem cells alleviates paraplegia in a rat model of aortic occlusion/reperfusion-induced spinal cord infarction","authors":"Eisaku Takahara ,&nbsp;Kota Kamizato ,&nbsp;Manabu Kakinohana ,&nbsp;Hiroshi Sunami ,&nbsp;Yuya Kise ,&nbsp;Kojiro Furukawa ,&nbsp;Edward Hosea Ntege ,&nbsp;Yusuke Shimizu","doi":"10.1016/j.reth.2024.08.005","DOIUrl":"10.1016/j.reth.2024.08.005","url":null,"abstract":"<div><h3>Background</h3><p>Thoracoabdominal periprocedural occlusion/reperfusion injury of the spinal cord (SCII/R) can lead to devastating paraplegia, underscoring the critical need for effective interventions. However, our knowledge of optimal medical strategies and their efficacy remains limited. Preclinical investigations have shown promise in harnessing adult stem cells, including pluripotent and multipotent stem cells such as mesenchymal stem cells (MSCs), to address SCII/R by enhancing neuro-inflammation, axonal growth, and myelination. Particularly, growth factors derived from adipose tissue-derived MSCs (ADSCs) have been proposed to facilitate recovery. Despite advancements, achieving complete recovery remains a formidable challenge. Therefore, gaining a more profound insight into the role of ADSCs in alleviating SCII/R-induced paraplegia, including optimizing the delivery systems for therapies, is imperative.</p></div><div><h3>Materials and methods</h3><p>In this study, we assessed the impact of subpial allogeneic rat adipose tissue-derived MSCs (rADSCs) transplantation on paraplegia using a rat SCII/R model induced by ephemeral aortic occlusion, known as the Taira-Marsala model. rADSCs were isolated from adipose tissue of male Sprague-Dawley rats, cultured, characterized, and cryopreserved. One week following the induction of paraplegia, rADSCs (n = 6) or physiological saline (n = 6) were transplanted. Hind limb motor function was evaluated before treatment and at 3-, 7-, and 14-days post-treatment using the Basso-Beattie-Bresnahan scoring system.</p></div><div><h3>Results</h3><p>The rADSC-treated group demonstrated a significant improvement in hind limb motor function compared to the saline-treated group (p &lt; 0.05), with 5 out of 6 rats exhibiting enhanced motor function following treatment.</p></div><div><h3>Conclusions</h3><p>Our findings suggest that subpial rADSC engraftment may enhance SCII/R-induced paraplegia recovery. These initial results drive further research to validate this potential, understand the molecular mechanisms, and optimize therapies.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 611-619"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001408/pdfft?md5=5177fc068df17f0ab38cfdf027c380da&pid=1-s2.0-S2352320424001408-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector","authors":"Kazuto Kimura ,&nbsp;Masaya Tsukamoto ,&nbsp;Hiroko Sugisaki ,&nbsp;Miyuu Tanaka ,&nbsp;Mitsuru Kuwamura ,&nbsp;Yuki Matsumoto ,&nbsp;Genki Ishihara ,&nbsp;Kei Watanabe ,&nbsp;Mika Okada ,&nbsp;Mahito Nakanishi ,&nbsp;Kikuya Sugiura ,&nbsp;Shingo Hatoya","doi":"10.1016/j.reth.2024.08.012","DOIUrl":"10.1016/j.reth.2024.08.012","url":null,"abstract":"<div><p>Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research has not been reported. Here, we generated footprint-free fiPSCs derived from embryonic cells, as well as juvenile feline uterus-derived cells using Sendai virus vector harboring six feline-specific pluripotency-associated genes. The fiPSCs were confirmed to be of high quality with the potential to form teratomas including all three germ layers. Furthermore, our fiPSCs were maintained under feeder-free and chemically-defined conditions using StemFit® AK02N and recombinant laminin 511, iMatrix-511. Further research on fiPSCs could result in their widespread application in veterinary regenerative medicine, which could pave the way for their use in advanced regenerative medicine research for humans.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 708-716"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001494/pdfft?md5=f7008751271e272e08f82d2357e37fe8&pid=1-s2.0-S2352320424001494-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of endometrial stem cells encapsulated in alginate/gelatin hydrogel to treat of polycystic ovary syndrome","authors":"Fatemeh Kouchakzadeh ,&nbsp;Somayeh Ebrahimi-Barough ,&nbsp;Behrouz Aflatoonian ,&nbsp;Jafar Ai ,&nbsp;Fahime Mazaheri ,&nbsp;Fateme Montazeri ,&nbsp;Fatemeh Hajizadeh-Tafti ,&nbsp;Jalal Golzadeh ,&nbsp;Reza Naser ,&nbsp;Masoumeh Sepehri ,&nbsp;Seyed Mehdi Kalantar","doi":"10.1016/j.reth.2024.08.016","DOIUrl":"10.1016/j.reth.2024.08.016","url":null,"abstract":"<div><p>Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder in women, often leading to infertility due to anovulation. Recent advances suggest that endometrial stem cells (EnSCs) hold considerable promise for tissue regeneration, which could be pivotal in treating PCOS. To enhance the survival and stabilization of EnSCs within the ovary, the EnSCs were encapsulated in an injectable alginate/gelatin hydrogel (SC–H), which has excellent biocompatibility to support the survival of EnSCs. Polycystic ovary syndrome was induced in female Wistar rats using intraperitoneal injection of letrozole over 21 days. Then the rats were treated with SC, SC-H and clomiphene citrate for one-month post-PCOS induction. The effects of these treatments were evaluated based on changes in body and ovarian weights, inflammatory markers, endocrine profiles, and ovarian histology. The Induction of PCOS led to a significant increase in body and ovarian cyst weight, elevated serum levels of testosterone, luteinizing hormone (LH), and anti-Müllerian hormone (AMH), alongside reduced follicle-stimulating hormone (FSH) and progesterone levels. Histologically, there was a decrease in granulosa cells, immature follicles, and corpus luteum numbers. Treatment with SC and SC-H significantly mitigated these alterations, indicating improved PCOS conditions. Our findings demonstrate that SC and SC-H treatments can effectively ameliorate the symptoms of letrozole-induced PCOS in rats, primarily through their anti-inflammatory effects. This study lays the groundwork for potential clinical applications of EnSCs encapsulated in alginate/gelatin hydrogel as a novel therapeutic strategy for PCOS, highlighting the importance of biomaterials in stem cell-based therapies.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 693-707"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001524/pdfft?md5=a5775bab8d167247e83a9a87641ff0f9&pid=1-s2.0-S2352320424001524-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative medicine in cardiovascular disease","authors":"Takasumi Goto ,&nbsp;Yuki Nakamura ,&nbsp;Yoshito Ito ,&nbsp;Shigeru Miyagawa","doi":"10.1016/j.reth.2024.09.004","DOIUrl":"10.1016/j.reth.2024.09.004","url":null,"abstract":"<div><div>Owing to the rapid increase in the number of people with severe heart failure, regenerative medicine is anticipated to play a role in overcoming the limitations inherent in existing surgical interventions. There are essentially two types of cardiac regenerative therapies for a failing heart. Cellular regenerative therapies using various stem cells improve the functional recovery of the heart mainly by cytokine paracrine effects. The implantation of induced pluripotent stem cell–derived cardiomyocytes can contribute not only to the inhibition of adverse heart remodeling by paracrine effects but also to the supply of newly born functional myocytes with the recipient myocardium as “mechanically working cells.” Cell transplantation, including autologous myoblast transplantation, reduces heart failure exacerbations and benefits patients without the need for other treatment options. Although cellular therapy is currently the mainstream approach, it requires an in-house cell-processing center with an aseptic environment. In addition, these stem cells are usually introduced via several invasive delivery methods, including intracoronary administration, and cellular sheet implantation. Simplifying the culture methods for these cells is a crucial problem that needs to be resolved.</div><div>Drug-induced regenerative therapy is another option that enhances self-endogenous regenerative systems in the human body and does not require invasive methods or cell cultures. Therefore, drug-induced regenerative therapies may overcome the disadvantages of these cellular therapies. The purpose of this report is to summarize cell transplantation therapy in the cardiovascular system and regenerative therapy for heart failure using an autologous endogenous regenerative system.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 859-866"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat","authors":"Mozafar Khazaei ,&nbsp;Mohammad Rasool Khazaei ,&nbsp;Sepideh Kadivarian ,&nbsp;Shahid Muhsin Hama ,&nbsp;Hevar Hassan Hussein ,&nbsp;Hojjat Haghighimanesh ,&nbsp;Leila Rezakhani","doi":"10.1016/j.reth.2024.09.017","DOIUrl":"10.1016/j.reth.2024.09.017","url":null,"abstract":"<div><div>Tissue regeneration in many skin defects is progressing with new treatments in recent years. Tissue engineering with the use of scaffolds offers more versatile and faster solutions in treatment. Extracellular matrix (ECM) and its three-dimensional (3D) network structure as a biological bond by imitating the tissue microstructure has been used for tissue repair, which can answer many existing challenges. Vitamin A, which comes in several forms such as retinols, retinals, and retinoic acids, is a necessary vitamin that is crucial for wound healing. In this research, sheep kidney capsule tissue decellularized with sodium dodecyl sulfate (SDS) containing different doses of vitamin A has been used as an ECM in skin tissue engineering. The above scaffold was evaluated in terms of properties such as biocompatibility, analysis of mechanical properties, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), hydrophilicity, antibacterial, and cell adhesion. The findings reported suitable properties for wound dressing, especially at a dose of 15,000 U/ml vitamin A for this scaffold. Then, the above scaffold was evaluated on the full-thickness wound model in rat, which showed good wound contraction, and increased VEGF factor. It showed a decrease in IL-1β level. Therefore, the use of the above-mentioned decellularized scaffold in combination with medicinal agents effective in wound healing can be introduced for further pre-clinical studies.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 867-878"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}