碱性成纤维细胞生长因子对软骨到骨的影响：多指衍生软骨细胞体内软骨形成的时间过程组织学分析

IF 3.4 3区环境科学与生态学 Q3 CELL & TISSUE ENGINEERING

Regenerative Therapy Pub Date : 2025-01-31 DOI:10.1016/j.reth.2025.01.013

Michiyo Nasu , Shinichiro Takayama , Rin Amagase , Akihiro Umezawa

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引用次数: 0

摘要

软骨组织是人体中普遍存在的重要结缔组织。然而，关于软骨细胞生长的限制和碱性FGF （bFGF）在体内的作用的信息很少。本研究旨在探讨bFGF对NOD/ shiscid il - 2r - γ缺失小鼠（NOG小鼠）移植培养的骨骺软骨源性软骨细胞生长速率、细胞学和形态学的影响。方法从多指畸形患者的指骺软骨中分离软骨细胞，分别在添加bFGF （bFGF处理过的细胞）和不添加bFGF （bFGF未处理过的细胞）的培养基中培养。这些培养的细胞被皮下植入NOG小鼠的背部。结果bfgf处理后的细胞生长速率高于未处理的细胞。软骨在植入两周后形成。经bfgf处理的细胞生成的软骨（下文简称“bt -软骨”）比未经bfgf处理的细胞生成的软骨（下文简称“but -软骨”）体积更大，重量更重。10周后，bt软骨呈指数增长。结论：与未处理的细胞相比，bfgf处理的细胞具有更高的增殖和分化能力。这项研究的结果可能会导致通过使用骨骺软骨细胞来产生新的骨和软骨替代疗法。

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Effects of basic fibroblast growth factor on cartilage to bone: Time-course histological analysis of in vivo cartilage formation from polydactyly-derived chondrocytes

Introduction

Cartilage tissue is important in the human body as a ubiquitous connective tissue. However, information on the limits of chondrocyte growth and the effects of basic FGF (bFGF) in vivo is scarce. This study aims to investigate the effects of bFGF on the growth rate, cytology, and morphology of implanted cultured epiphyseal cartilage-derived chondrocytes into NOD/Shi-scid IL-2Rγ^null mice (NOG mice).

Methods

Chondrocytes were isolated from the epiphyseal cartilage derived from the digits of polydactyly patients and were cultivated in a medium supplemented with bFGF (bFGF-treated cells) and without bFGF (bFGF-untreated cells). These cultivated cells were subcutaneously implanted into the backs of NOG mice.

Results

bFGF-treated cells exhibited a higher growth rate than bFGF-untreated cells. Cartilage was formed after two weeks of implantation. The cartilages generated by bFGF-treated cells (denoted as “BT-cartilage” hereafter) were larger in size and heavier in weight than those cartilages generated by bFGF-untreated cells (denoted as “BUT-cartilage” hereafter). BT-cartilage grew exponentially after 10 weeks.

Conclusions

From these results, bFGF-treated cells exhibited increased ability for both proliferation and differentiation compared to bFGF-untreated cells. The results of this study may lead to the generation of new alternative therapies for bone and cartilage through the use of epiphyseal chondrocytes.

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来源期刊

Regenerative Therapy Engineering-Biomedical Engineering

CiteScore

6.00

自引率

2.30%

发文量

106

审稿时长

49 days

期刊介绍： Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.