Regenerative Therapy最新文献

{"title":"Status and prospects for the development of regenerative therapies for corneal and ocular diseases","authors":"Hiroshi Takayanagi ,&nbsp;Ryuhei Hayashi","doi":"10.1016/j.reth.2024.09.001","DOIUrl":"10.1016/j.reth.2024.09.001","url":null,"abstract":"<div><p>Among the regenerative therapies being put into clinical use, the field of corneal regenerative therapy is one of the most advanced, with several regulatory approved products. This article describes the progress from initial development through to clinical application in the eye field, with a particular focus on therapies for corneal epithelial and endothelial diseases that have already been regulatory approved as regenerative therapy products. The applications of regenerative therapy to the corneal epithelium were attempted and confirmed earlier than other parts of the cornea, following advancements in basic research on corneal epithelial stem cells. Based on these advances, four regenerative therapy products for corneal epithelial disease, each employing distinct cell sources and culture techniques, have been commercialized since the regulatory approval of Holoclar® in Italy as a regenerative therapy product for corneal epithelial disease in 2015. Corneal endothelial regenerative therapy was started by the development of an <em>in vitro</em> method to expand corneal endothelial cells which do not proliferate in adults. The product was approved in Japan as Vyznova® in 2023. The development of regenerative therapies for retinal and ocular surface diseases is actively being pursued, and these therapies use somatic stem cells and pluripotent stem cells (PSCs), especially induced pluripotent stem cells (iPSCs). Accordingly, the eye field is anticipated to play a pioneering role in regenerative therapy development going forward.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 819-825"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001627/pdfft?md5=f847733a1503529e24332b9703c41d32&pid=1-s2.0-S2352320424001627-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142239629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo biocompatibility assessment of 3D printed bioresorbable polymers for brain tissue regeneration. A feasibility study","authors":"Julien Clauzel ,&nbsp;Nina Colitti ,&nbsp;Maylis Combeau ,&nbsp;Wafae Labriji ,&nbsp;Lorenne Robert ,&nbsp;Adrien Brilhault ,&nbsp;Carla Cirillo ,&nbsp;Franck Desmoulin ,&nbsp;Isabelle Raymond-Letron ,&nbsp;Isabelle Loubinoux","doi":"10.1016/j.reth.2024.10.004","DOIUrl":"10.1016/j.reth.2024.10.004","url":null,"abstract":"<div><h3>Introduction</h3><div>The limited capacity of brain tissue to regenerate after acute injury, hampered by cell death, edema and inflammation, has led to an interest in promising and innovative approaches such as implantable regenerative scaffolds designed to improve brain plasticity. Leveraging the capabilities of bioprinting, these scaffolds can be tailored to match the intricate architecture of the brain.</div></div><div><h3>Methods</h3><div>In this methodological study, we performed in vivo biocompatibility assessments after a brain lesion on three distinct bioeliminable or bioresorbable materials: Poly(ethylene glycol) diacrylate (PEGDA), Polycaprolactone (PCL) and a PEGDA mixed with gelatin methacrylate (PEGDA-GelMA).</div></div><div><h3>Results</h3><div>A scaffold with a complex shape was printed with patterns, spatial resolution and porosity adapted to cerebral cortex reconstruction. In vivo evaluations were complemented by behavioral monitoring, affirming the safety of these materials. High-resolution T2 MRI imaging effectively captured scaffold structures and demonstrated their non-invasive utility in monitoring degradability. ASL MRI imaging quantified cerebral blood flow and was positively and significantly correlated with lectin immunofluorescent labeling. It may be used to non-invasively monitor progressive revascularization of implants.</div><div>PEGDA produced an intense foreign-body response, encapsulated by a fibro-inflammatory barrier. On the other hand, PCL provoked a controlled inflammatory reaction and facilitated cell migration into the scaffold, although it induced a fibrotic response around PCL fibers. Conversely, the PEGDA-GelMA composite emerged as a promising candidate for intracerebral implantation. It facilitated the creation of a permissive glial layer, while also inducing neovascularization and attracting neuronal progenitors.</div></div><div><h3>Conclusion</h3><div>Behavior, MRI monitoring and histology allowed a thorough following of biomaterial biocompatibility. The collective findings position PEGDA-GelMA as a convincing biomaterial option as a basis for treating severe brain lesions, offering new avenues in the search for effective treatments.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 941-955"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory insights on advanced CAR-T cell products, AAV-based Gene therapies, and medical care/practice in cell and Gene therapies: Report from the 6th Asia partnership conference of regenerative medicine- April 20, 2023","authors":"Hiroshi Karasawa ,&nbsp;Hirokuni Mizoguchi ,&nbsp;Bryan Choi ,&nbsp;Chia-Ling Hsieh ,&nbsp;Masaaki Miyano ,&nbsp;Yuu Moriya ,&nbsp;Sasikumar Muthusamy ,&nbsp;Koji Takakura ,&nbsp;Kunihiko Suzuki ,&nbsp;Yoshie Tsurumaki ,&nbsp;Takeshi Watanabe ,&nbsp;Karen Wen ,&nbsp;Tomohiro Yoneda ,&nbsp;Ta-Tung Yuan ,&nbsp;Masayuki Nomura","doi":"10.1016/j.reth.2024.09.015","DOIUrl":"10.1016/j.reth.2024.09.015","url":null,"abstract":"<div><div>The 6th Asia Partnership Conference of Regenerative Medicine (APACRM) was held in person with online on April 20, 2023, to promote the regulatory harmonization of regenerative medicine products throughout Asia. Recognizing domestic regulatory guidelines within each country and region and the underlying rationales are important initial steps toward harmonizing regulations. The 6th APACRM featured an open dialog regarding non-clinical evaluation for advanced CAR-T products, regulation of clinical trials for AAV-based gene therapies, and cell and gene therapies provided to patients as medical care/medical practices without market authorization through presentations from the industry and panel discussions with regulatory agencies. The latest updates on regenerative medicine in each country and region are introduced. This paper summarizes the proceedings of the 6th APACRM for public dissemination to foster future discussions.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 967-980"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic effect of sufficient oxygen-rich PRP injection in facial rejuvenation by multiple objective evaluations in 26 cases","authors":"Jianyun Lu ,&nbsp;Tong Zhang ,&nbsp;Lu Zhou ,&nbsp;Xiaoliang Tong ,&nbsp;Rong Gui ,&nbsp;Ling Jiang ,&nbsp;Zhen Tang ,&nbsp;Yunfeng Fu ,&nbsp;Guosheng Zhao ,&nbsp;Jinrong Zeng ,&nbsp;Lihua Gao","doi":"10.1016/j.reth.2024.05.013","DOIUrl":"https://doi.org/10.1016/j.reth.2024.05.013","url":null,"abstract":"<div><h3>Background</h3><p>Ozone can enhance the expression of some growth factors (GFs) in platelet rich plasma (PRP), recent study showed oxygen-rich PRP (ozonized PRP) have better therapeutic effects on bone and joint diseases. PRP injection has been widely used in the treatment of facial rejuvenation, but the efficacy of sufficient oxygen-rich PRP in facial rejuvenation has not been studied.</p></div><div><h3>Objective</h3><p>Firstly, we examined whether ozone treatment can increase the concentration of GFs of PRP in vitro. And then a variety of subjective and objective detection methods were used to evaluate the effect of sufficient(10–12 mL each time for the injection of face and neck) oxygen-rich (ozonized PRP) PRP injection in facial rejuvenation by follow-up for 6 months. At last, we investigated the satisfaction, side effects and pain score of the treatment through a questionnaire survey.</p></div><div><h3>Methods</h3><p>The concentration of main GFs in PRP treated with different dose of ozone in vitro was measured by ELISA. Clinical picture, the collagen thickness of dermis by reflectance confocal microscope(RCM), skin conditions (including spots, ultraviolet (UV) spots, brown spots, red area, pores, wrinkles, texture and porphyrin) by VISIA were collected before treatment and each month follow-up visit after treatment until 6-month follow-up period was finished. Patients’ satisfaction, side effects and pain score were collected at the end of follow-up period.</p></div><div><h3>Results</h3><p>PRP treated by high-dose ozone (57 μg/mL, ozone/PRP volume ratio:1/1) in vitro showed a significant increase in endothelial growth factor (EGF) and transforming growth factor-β (TGF-β) compared to baseline(<em>P</em> &lt; 0.05). Collagen thickness of forehead, cheek and neck improved significantly compare to the baseline until to the 6 months after treatment. Spots, UV spots, brown spots, red area and texture improved significantly compare to the baseline(<em>P</em> &lt; 0.05). All of participants reported improvement and have a median pain score of 4.19. No serious adverse events were observed.</p></div><div><h3>Conclusions</h3><p>Ozone treatment can increase the concentration of GFs such as EGF and TGF-β in PRP in vitro. Sufficient oxygen-rich PRP injection may be an effective and promising method to treat facial rejuvenation.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 213-218"},"PeriodicalIF":4.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001007/pdfft?md5=1ff0a7a6ccfeadd3bdfc811c69bc41c9&pid=1-s2.0-S2352320424001007-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High efficiency protocol for platelet derived fibrin gel loaded with mesenchymal stromal cells extracellular vesicles","authors":"Enrico Ragni ,&nbsp;Paola De Luca ,&nbsp;Simona Landoni ,&nbsp;Federico Valli ,&nbsp;Leonardo Mortati ,&nbsp;Silvia Palombella ,&nbsp;Giuseppe Talò ,&nbsp;Matteo Moretti ,&nbsp;Laura de Girolamo","doi":"10.1016/j.reth.2024.06.020","DOIUrl":"https://doi.org/10.1016/j.reth.2024.06.020","url":null,"abstract":"<div><h3>Introduction</h3><p>Extracellular vesicles from mesenchymal stromal cells (MSC-EVs) are potent stimulators of naïve cartilage and their injection is studied in clinical trials for cartilage lesions, since often cartilage repaired with conventional approaches is incomplete or less performant leading to joint degeneration. The main pitfall of these innovative approaches is the high EVs dispersion into the joint cavity and consequent low concentration at lesion site. Thus, biological scaffolds for concentration of EVs where needed might be a promising option. This work aimed at producing an enhanced platelet-derived fibrin gel loaded with adipose-derived MSCs (ASCs)-EVs.</p></div><div><h3>Methods</h3><p>EVs’ embedment efficiency in platelet gel, their release and incorporation in OA chondrocytes and cartilage explants were monitored by flow cytometry, microfluidic approaches, scansion electron microscopy and real-time quantitative multimodal nonlinear optics imaging. The effect of released EVs was tested in OA chondrocytes by gene expression studies.</p></div><div><h3>Results</h3><p>A protocol ensuring high incorporation EVs efficiency in platelet gels was defined, relying on a one-step modification of the standard procedure used in current clinical practice. Trapped EVs were released continuously for up to 4 weeks and uptaken in pathologic chondrocytes and cartilage explants. The release of the EVs-loaded platelet gel had stronger and synergic anti-inflammatory/matrix remodelling effects with respect to both EVs per se and unloaded gel released products.</p></div><div><h3>Conclusions</h3><p>These results suggest the feasibility of producing a platelet gel loaded with MSC-EVs at high efficiency that can be used as an enhanced tool to foster chondrocyte homeostasis, a key requisite for proper cartilage healing.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 442-457"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001275/pdfft?md5=9883c821b10a2bce31c3ec42b29b40a9&pid=1-s2.0-S2352320424001275-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141583232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study","authors":"Chengjin Chen ,&nbsp;Hui Zhao ,&nbsp;Wenlu Zhang ,&nbsp;Xuelan Hong ,&nbsp;Shengjie Li ,&nbsp;Saeed Rohani","doi":"10.1016/j.reth.2024.06.018","DOIUrl":"10.1016/j.reth.2024.06.018","url":null,"abstract":"<div><p>Diabetic wounds pose an enduring clinical hurdle, marked by delayed recovery, persistent inflammation, and an elevated susceptibility to infections. Conventional treatment approaches often fall short of delivering optimal outcomes, prompting the exploration of innovative methods to enhance the healing process. Electrospun wound dressings offer superior healing, controlled drug release, enhanced cell proliferation, biocompatibility, high surface area, and antimicrobial properties. In the current study, polycaprolactone/gelatin-based nanofibrous wound dressings were developed for the delivery of Wharton's jelly stem cells and curcumin into the diabetic wounds bed. Curcumin was loaded into the polycaprolactone/gelatin solution and electrospun to produce curcumin-loaded scaffolds. In vitro experiments including scanning electron microscopy, cell viability assay, release assay, hemocompatibility assay, cell proliferation assay, and antibacterial assay were utilized to characterize the delivery system. Then, curcumin-loaded scaffolds were seeded with 30,000 Wharton's jelly stem cells and implanted into a rat model of diabetic wounds. Study showed that the scaffolds containing both Wharton's jelly stem cells and curcumin significantly improved diabetic wound closure (86.32 3.88% at the end of 14th day), augmented collagen deposition, and improved epithelial tissue formation. Gene expression studies showed that VEGF and IGF genes were significantly upregulated by the co-delivery system. Our developed system may have augmented diabetic wound healing via upregulating pro-healing genes.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 547-556"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001263/pdfft?md5=e3b1d760f124180ff507600d7d5d875e&pid=1-s2.0-S2352320424001263-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141950281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative medicine in Obstetrics & Gynecology: Current status under the Act on the Safety of Regenerative Medicine in Japan","authors":"Satoshi Hosoya ,&nbsp;Sena Awano-Kim ,&nbsp;Ryo Yokomizo ,&nbsp;Yuichiro Ukon ,&nbsp;Kazuki Morita ,&nbsp;Yuta Kasahara ,&nbsp;Hiroshi Kishi ,&nbsp;Aikou Okamoto","doi":"10.1016/j.reth.2024.08.003","DOIUrl":"10.1016/j.reth.2024.08.003","url":null,"abstract":"<div><h3>Introduction</h3><p>While the provision of unapproved regenerative medicine has been problematic worldwide, few studies have examined the implementation status of regenerative medicine (RM) in the specific field. This study aimed to determine the current status of therapy and clinical research in the obstetrics and gynecology (OBGYN) in Japan under the Act on the Safety of Regenerative Medicine (RM Act).</p></div><div><h3>Methods</h3><p>Detailed data were extracted from publicly available websites provided by the Ministry of Health, Labour, and Welfare. We extracted descriptive details, including risk classification of the RM Act, modality, target disease, locality, institution, and administration route. For therapy, the price for each modality was evaluated.</p></div><div><h3>Results</h3><p>The total number of therapeutic provision plans in OBGYN (1.9% of RM in Japan) are classified as Class II (moderate) risk. Most were administered in clinics in urban areas for treating endometrial or ovarian infertility by locally administering platelet-rich plasma (PRP) or autologous mesenchymal stem cells (MSCs). The price using MSCs is approximately eight times more expensive that of those involving PRP (1832.1 ± 1139.8 vs 240.8 ± 106.5 thousand yen, p &lt; 0.0001). Regarding research, four plans (2.2%) were submitted to target implantation failure and advanced gynecological cancer using autologous lymphocytes, dendritic cells, or MSCs.</p></div><div><h3>Conclusion</h3><p>The RM Act permits knowledge of the current status of regenerative medicine even for unapproved uses in a specific clinical field. The study findings shall prompt a worldwide discussion regarding the required regulations for therapy and clinical research of RM.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 564-570"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001391/pdfft?md5=03dca65353bf40aa19ebdbb6e87d1e11&pid=1-s2.0-S2352320424001391-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing engineered muscle tissues utilizing standard cell culture plates and mesenchymal stem cell-conditioned medium","authors":"Yihao Wen ,&nbsp;Jia Tian ,&nbsp;Juan Li ,&nbsp;Xiangming Na ,&nbsp;Ziyi Yu ,&nbsp;Weiqing Zhou","doi":"10.1016/j.reth.2024.08.011","DOIUrl":"10.1016/j.reth.2024.08.011","url":null,"abstract":"<div><p>The construction of engineered muscle tissues that resemble the function and microstructure of human muscles holds significant promise for various applications, including disease modeling, regenerative medicine, and biological machines. However, current muscle tissue engineering approaches often rely on complex equipment which may limit their accessibility and practicality. Herein, we present a convenient approach using a standard 24-well cell culture plate to construct a platform to facilitate engineered muscle tissues formation and culture. Using this platform, engineered muscle tissue with differentiation characteristics can be manufactured in large quantities. Additionally, the mesenchymal stem cell conditioned medium was utilized to promote the formation and functionality of the engineered muscle tissues. The resulting tissues comprised a higher cell density and a better differentiation effect in the tissues. Taken together, this study provides a simple, convenient, and effective platform for studying muscle tissue engineering.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 683-692"},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001482/pdfft?md5=e4f0ae84a7a76d08342f88460fc8a953&pid=1-s2.0-S2352320424001482-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring gellan gum-based hydrogels for regenerating human embryonic stem cells in age-related macular degeneration therapy: A literature review","authors":"Mthabisi Talent George Moyo ,&nbsp;Terin Adali ,&nbsp;Pinar Tulay","doi":"10.1016/j.reth.2024.05.018","DOIUrl":"https://doi.org/10.1016/j.reth.2024.05.018","url":null,"abstract":"<div><p>Age-related macular degeneration (AMD) is a progressive ocular disease marked by the deterioration of retinal photoreceptor cells, leading to central vision decline, predominantly affecting the elderly population worldwide. Current treatment modalities, such as anti-VEGF agents, laser therapy, and photodynamic therapy, aim to manage the condition, with emerging strategies like stem cell replacement therapy showing promise. However, challenges like immune rejection and cell survival hinder the efficacy of stem cell interventions. Regenerative medicine faces obstacles in maximizing stem cell potential due to limitations in mimicking the dynamic cues of the extracellular matrix (ECM) crucial for guiding stem cell behaviour. Innovative biomaterials like gellan gum hydrogels offer tailored microenvironments conducive to enhancing stem cell culture efficacy and tissue regeneration. Gellan gum-based hydrogels, renowned for biocompatibility and customizable mechanical properties, provide crucial support for cell viability, differentiation, and controlled release of therapeutic factors, making them an ideal platform for culturing human embryonic stem cells (hESCs). These hydrogels mimic native tissue mechanics, promoting optimal hESC differentiation while minimizing immune responses and facilitating localized delivery. This review explores the potential of Gellan Gum-Based Hydrogels in regenerative AMD therapy, emphasizing their role in enhancing hESC regeneration and addressing current status, treatment limitations, and future directions.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 235-250"},"PeriodicalIF":4.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001056/pdfft?md5=c27fc2ce1ecb1407658859499c7582ee&pid=1-s2.0-S2352320424001056-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141303406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture","authors":"Zhi Liu ,&nbsp;Shunxin Jin ,&nbsp;Dapeng Cheng ,&nbsp;Hao Chen ,&nbsp;Yuxiang Wang ,&nbsp;Chao Ji ,&nbsp;Zhenzhen Yan ,&nbsp;Xiao Fang ,&nbsp;Shichu Xiao ,&nbsp;Xinling Bi","doi":"10.1016/j.reth.2024.05.014","DOIUrl":"https://doi.org/10.1016/j.reth.2024.05.014","url":null,"abstract":"<div><h3>Introduction</h3><p>With the increasing emphasis on the use of nonanimal ingredients in clinical care, studies have proposed the use of TrypLE™ as an alternative to trypsin. However, previous research has reported insufficient cell yield and viability when using TrypLE to isolate skin cells compared to the dispase/trypsin-EDTA method. This study aimed to propose an improved method for increasing the yield and viability of cells isolated by TrypLE and to evaluate isolated keratinocytes and melanocytes.</p></div><div><h3>Methods</h3><p>Foreskin tissues were isolated to keratinocytes and melanocytes using the trypsin-EDTA protocol and our modified TrypLE protocol. The yield and viability of freshly isolated cells were compared, the epidermal residue after cell suspension filtration was analyzed histologically, and the expression of cytokeratin 14 (CK14) and Melan-A was detected by flow cytometry. After cultivation, keratinocytes and melanocytes were further examined for marker expression and proliferation. A coculture model of melanocytes and HaCaT cells was used to evaluate melanin transfer.</p></div><div><h3>Results</h3><p>The yield, viability of total cells and expression of the keratinocyte marker CK14 were similar for freshly isolated cells from both protocols. No differences were observed in the histologic analysis of epidermal residues. Moreover, no differences in keratinocyte marker expression or melanocyte melanin transfer function were observed after culture. However, melanocytes generated using the TrypLE protocol exhibited increased Melan-A expression and proliferation in culture.</p></div><div><h3>Conclusion</h3><p>Our TrypLE protocol not only solved the problems of insufficient cell yield and viability in previous studies but also preserved normal cell morphology and function, which enables the clinical treatment of depigmentation diseases.</p></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"Pages 203-212"},"PeriodicalIF":4.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352320424001019/pdfft?md5=342e12c45f82405eb1d0ec3b4b3a98c8&pid=1-s2.0-S2352320424001019-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}