Regenerative Therapy最新文献

{"title":"Interplay between pioneer transcription factors and epigenetic modifiers in cell reprogramming","authors":"Gerardo Mirizio ,&nbsp;Samuel Sampson ,&nbsp;Makiko Iwafuchi","doi":"10.1016/j.reth.2024.12.014","DOIUrl":"10.1016/j.reth.2024.12.014","url":null,"abstract":"<div><div>The generation of induced pluripotent stem cells (iPSCs) from differentiated somatic cells by Yamanaka factors, including pioneer transcription factors (TFs), has greatly reshaped our traditional understanding of cell plasticity and demonstrated the remarkable potential of pioneer TFs. In addition to iPSC reprogramming, pioneer TFs are pivotal in direct reprogramming or transdifferentiation where somatic cells are converted into different cell types without passing through a pluripotent state. Pioneer TFs initiate a reprogramming process through chromatin opening, thereby establishing competence for new gene regulatory programs. The action of pioneer TFs is both influenced by and exerts influence on epigenetic regulation. Despite significant advances, many direct reprogramming processes remain inefficient, which limits their reliability for clinical applications. In this review, we discuss the molecular mechanisms underlying pioneer TF-driven reprogramming, with a focus on their interactions with epigenetic modifiers, including Polycomb repressive complexes (PRCs), nucleosome remodeling and deacetylase (NuRD) complexes, and the DNA methylation machinery. A deeper understanding of the dynamic interplay between pioneer TFs and epigenetic modifiers will be essential for advancing reprogramming technologies and unlocking their full clinical potential.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 246-252"},"PeriodicalIF":3.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel preparation of laponite based theranostic silver nanocomposite for drug delivery, radical scavenging and healing efficiency for wound care management after surgery","authors":"Lizhen Long ,&nbsp;Xiaoan Ma ,&nbsp;Hua Zhang ,&nbsp;Changgong Lan","doi":"10.1016/j.reth.2024.12.004","DOIUrl":"10.1016/j.reth.2024.12.004","url":null,"abstract":"<div><div>In this work, laponite (LAP) was used to develop the silver (Ag) based nanocomposite for improved anti-bacterial action and wound healing properties. The amphiphilic co-polymers such as PLGA polymer was embedded with the surface of LAP molecules and polyethyleneimine (PEI) through the interaction of hydrophobic binding and it was formed as LAP/PLA-PEG/PEI formulation through the coupling chemistry. The Ag nanoparticles was loaded into formulation to develop LAP/PLA-PEG/PEI/Ag nanocomposite and characterized by different analytical techniques. The UV–Vis and XRD results report that the nanocomposite has shown Surface Plasmon Resonance (SPR) peak at 415 nm for AgNPs particles and provide the strong intensity peak along with 48 nm particle size, respectively. The HR-TEM image showed that the LAP/PLA-PEG/PEI/Ag nanocomposite has shown crystal lattice with spherical morphology. In addition, the synthesized nanocomposite requested in the swelling along with degradation, radial scavenging activity and drug releasing behaviour by <em>in vitro</em> method. The anti-bacterial analysis results showed that the nanocomposite have greater bacterial inhibition. Moreover, <em>In vitro</em> cell model and <em>In vivo</em> wound healing studies confirmed that nanocomposite have significant biocompatibility and healing properties. Importantly, <em>in vivo</em> histological analyses confirmed the effective wound healing effect of nanocomposite by complete re-epithelialization, formation of new granulation tissue and blood vessels. These observations provide strong evidences to use this novel nanoformulation for wound healing application with anti-microbial action.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 235-245"},"PeriodicalIF":3.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline magnetic resonance imaging findings associated with short-term clinical outcomes after intraarticular administration of autologous adipose-derived stem cells for knee osteoarthritis","authors":"Ryota Yamagami ,&nbsp;Tomohiro Terao ,&nbsp;Taro Kasai ,&nbsp;Hisatoshi Ishikura ,&nbsp;Masaki Hatano ,&nbsp;Junya Higuchi ,&nbsp;Shuichi Yoshida ,&nbsp;Yusuke Arino ,&nbsp;Ryo Murakami ,&nbsp;Masashi Sato ,&nbsp;Yuji Maenohara ,&nbsp;Yuma Makii ,&nbsp;Tokio Matsuzaki ,&nbsp;Keita Inoue ,&nbsp;Shinsaku Tsuji ,&nbsp;Sakae Tanaka ,&nbsp;Taku Saito","doi":"10.1016/j.reth.2024.12.012","DOIUrl":"10.1016/j.reth.2024.12.012","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to determine the association between the baseline magnetic resonance imaging (MRI) findings and clinical outcomes after articular injection of adipose-derived mesenchymal stem cells (ASCs) for knee osteoarthritis (KOA).</div></div><div><h3>Methods</h3><div>This retrospective study included 149 patients with varus-type KOA treated with a single intraarticular ASC injection. All patients underwent a MRI evaluation before treatment. Patients were categorized following the MRI Osteoarthritis Knee Score (MOAKS) system cartilage score into the mild, moderate, or severe KOA groups. Additionally, joint effusion and synovitis, bone marrow lesions (BMLs), and meniscal extrusions were graded with the MOAKS. Knee Osteoarthritis Outcome Score (KOOS) was obtained at baseline, 1-, 3-, 6-, and 12-month posttreatment. The responder rate in the Outcome Measures in Arthritis Clinical Trials-Osteoarthritis Research Society International was assessed with the KOOS. Multivariate logistic regression analyses were conducted to determine factors associated with the responder rate.</div></div><div><h3>Results</h3><div>All KOOS subscales significantly enhanced with the greatest improvement from baseline to 6 months which plateaued between 6 and 12 months. The responder rate was 65.4 % in the mild/moderate KOA compared to 35.2 % in the severe KOA at 12 months. Lower OA grade (odds ratio [OR]: 0.52; 95 % confidence interval (CI): 0.31–0.88; <em>P</em> = 0.015), smaller BMLs in medial femoral condyle (OR: 0.36; 95 % CI: 0.14–0.94; <em>P</em> = 0.037), and less meniscal extrusion (OR: 0.31; 95 % CI: 0.11–0.89; <em>P</em> = 0.029) were associated with higher responder rate at 6 months in multivariable logistic regression analysis. The factors associated with higher responder rate at 12 months included lower OA grade (OR: 0.42; 95 % CI: 0.25–0.73; <em>P</em> = 0.002) and younger age (OR: 1.04; 95 % CI: 1.00–1.08; <em>P</em> = 0.042).</div></div><div><h3>Conclusions</h3><div>ASC treatment for KOA enhanced short-term clinical outcomes. MRI findings, including cartilage lesions, BMLs, and meniscal extrusion, were associated with responder rate, helping physicians identify which patients may benefit from this therapy.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 227-234"},"PeriodicalIF":3.4,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11741093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of GATA4 in mesenchymal stem cell senescence: A new frontier in regenerative medicine","authors":"M. Arockia Babu ,&nbsp;Renuka Jyothi S ,&nbsp;Irwanjot Kaur ,&nbsp;Sachin Kumar ,&nbsp;Naveen Sharma ,&nbsp;M. Ravi Kumar ,&nbsp;Pranchal Rajput ,&nbsp;Haider Ali ,&nbsp;Gaurav Gupta ,&nbsp;Vetriselvan Subramaniyan ,&nbsp;Ling Shing Wong ,&nbsp;Vinoth Kumarasamy","doi":"10.1016/j.reth.2024.11.017","DOIUrl":"10.1016/j.reth.2024.11.017","url":null,"abstract":"<div><div>The Mesenchymal Stem Cell (MSC) is a multipotent progenitor cell with known differentiation potential towards various cell lineage, making it an appealing candidate for regenerative medicine. One major contributing factor to age-related MSC dysfunction is cellular senescence, which is the hallmark of relatively irreversible growth arrest and changes in functional properties. GATA4, a zinc-finger transcription factor, emerges as a critical regulator in MSC biology. Originally identified as a key regulator of heart development and specification, GATA4 has since been connected to several aspects of cellular processes, including stem cell proliferation and differentiation. Accumulating evidence suggests that the involvement of GATA4-nuclear signalizing in the process of MSC senescence-related traits may contribute to age-induced alterations in MSC behavior. GATA4 emerged as the central player in MSC senescence, interacting with several signaling pathways. Studies have shown that GATA4 expression is reduced with age in MSCs, which is associated with increased expression levels of senescence markers and impaired regenerative potential. At the mechanistic level, GATA4 regulates the expression of genes involved in cell cycle regulation, DNA repair, and oxidative stress response, thereby influencing the senescence phenotype in MSCs. The findings underscore the critical function of GATA4 in MSC homeostasis and suggest a promising new target to restore stem cell function during aging and disease. A better understanding of the molecular mechanisms that underlie GATA4 mediated modulation of MSC senescence would provide an opportunity to develop new therapies to revitalize old MSCs to increase their regenerative function for therapeutic purposes in regenerative medicine.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 214-226"},"PeriodicalIF":3.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MAPT-A152T mutation drives neuronal hyperactivity through Fyn-NMDAR signaling in human iPSC-Derived neurons: Insights into Alzheimer's pathogenesis","authors":"Maika Itsuno ,&nbsp;Hirokazu Tanabe ,&nbsp;Etsuko Sano ,&nbsp;Takashi Sasaki ,&nbsp;Chisato Oyama ,&nbsp;Hiroko Bannai ,&nbsp;Koichi Saito ,&nbsp;Kazuhiko Nakata ,&nbsp;Setsu Endoh-Yamagami ,&nbsp;Hideyuki Okano ,&nbsp;Sumihiro Maeda","doi":"10.1016/j.reth.2024.12.009","DOIUrl":"10.1016/j.reth.2024.12.009","url":null,"abstract":"<div><h3>Introduction</h3><div>Tau protein plays a pivotal role in the pathogenesis of Alzheimer's disease (AD) and in regulating neuronal excitability. Among tau-coding microtubule associated protein tau (<em>MAPT</em>) gene mutations, the A152T mutation is reported to increase the risk of AD and neuronal excitability in mouse models.</div></div><div><h3>Methods</h3><div>To investigate the effects of <em>MAPT</em> gene expression and its mutations on neuronal activity in human neurons, we employed genome editing technology to introduce the A152T or P301S mutations into induced pluripotent stem cells (iPSCs). We then differentiated them into excitatory and inhibitory neurons. As a control, iPSCs in which the <em>MAPT</em> gene was replaced with a fluorescent protein were also created.</div></div><div><h3>Results</h3><div>In excitatory neuronal cultures, the A152T mutation was found to enhance spontaneous neuronal activity and the association of tau and Fyn. However, in inhibitory neuron-enriched cultures, the A152T mutation did not affect neuronal activity. Inhibition of NMDA receptors (NMDAR) and the reduction of tau protein levels decreased neuronal excitability in both A152T/A152T and healthy control (WT/WT) excitatory neurons. In addition, the A152T mutation increased the interaction between tau and Fyn. These findings suggest that the tau-Fyn interaction plays a critical role in regulating neuronal activity under physiological conditions, while the A152T mutation enhances neuronal activity by strengthening this endogenous interaction between tau and Fyn. In addition, transcriptomic analysis revealed structural changes specific to excitatory neurons with the A152T mutation. Common changes observed in both A152T and P301S lines recapitulated a dedifferentiation phenotype, consistent with previous reports.</div></div><div><h3>Conclusions</h3><div>These data demonstrate that the A152T mutation in the <em>MAPT</em> gene increases neuronal excitability through the tau-Fyn-NMDAR pathway in excitatory neurons, shedding light on its role in AD pathogenesis.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 201-213"},"PeriodicalIF":3.4,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem cell therapy for bladder regeneration: A comprehensive systematic review","authors":"Ali Faegh ,&nbsp;Shima Jahani ,&nbsp;Fatemeh Chinisaz ,&nbsp;Hamoon Baghaei ,&nbsp;Masoumeh Majidi Zolbin","doi":"10.1016/j.reth.2024.12.005","DOIUrl":"10.1016/j.reth.2024.12.005","url":null,"abstract":"<div><div>Tissue engineering has been considered a potential choice for urinary system reconstruction. Here, we aim to a broad spectrum of employed stem cells in bladder regeneration by performing a comprehensive systematic review. In January 2024, we searched Scopus, PubMed, and Embase databases for studies that tried bladder regeneration by tissue engineering using stem cells. We excluded non-English studies, review articles, and manuscripts that met the other exclusion criteria. Among 43 included studies, comparative studies demonstrated the similar or superior potentiality of stem cells to regenerate tissues and improve bladder function compared with autologous cells. Furthermore, data suggest an increased use of bio-synthetic scaffolds and their appropriate bio-compatibility with stem cells. The evidence establishes that adipose-derived and bone marrow-derived mesenchymal stem cells are the most frequently used stem cells. And both are suitable for urothelium and smooth muscle formation along with the capability of bone marrow-derived mesenchymal stem cells for lamina propria formation. Additionally, the competency of smooth muscle-derived progenitor cells, urine-derived stem cells, umbilical mesenchymal SCs for smooth muscle and urothelium regeneration, and the capability of hair follicle stem cells for smooth muscle formation are demonstrated. Also, the superiority of endothelial progenitor cells for neo-vascularization and smooth muscle progenitor cells for neuron formation are demonstrated. In addition to adding growth factors to the culturing media, hypoxic conditions and intra-peritoneal incubation are introduced as promoter conditions that can improve histological and physiological components. Available evidence is limited, although it suggests the precious capability of stem cells for bladder regeneration.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 191-200"},"PeriodicalIF":3.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardizing decellularization protocols for optimized uterine tissue bioengineering","authors":"Edina Sehic ,&nbsp;Lucía de Miguel-Gómez","doi":"10.1016/j.reth.2024.12.011","DOIUrl":"10.1016/j.reth.2024.12.011","url":null,"abstract":"<div><div>Bioengineering is applied in different areas, including women's infertility management. Among other approaches, decellularized tissues are being used to treat uterine disorders causing infertility. Biomaterials made from decellularized tissue consist of tissue-specific extracellular matrix and, as acellular scaffolds, are thought to be immune inert. Hence, they are good grafting candidates to replace and regenerate excised damaged uterine tissue to cure infertility. However, decellularization approaches differ among species and research groups, posing challenges for comparison and standardization. The diversity in data reporting and studied properties of the resulting decellularized scaffold make it even more difficult, especially when the ultimate goal is clinical translation. Thus, this review aims to critically assess whole uterus decellularization studies, extracting and comparing their main results and conclusions. After carefully evaluating the reviewed studies, we noticed that the vast majority base the uterus decellularization success and resulting scaffold efficacy on the DNA removal efficacy, while other crucial aspects, including the extracellular matrix integrity or immunogenicity, are underestimated. Thus, this review further proposes practical points for what should be considered and how results can be reported in studies involving whole uterus decellularization to facilitate comparison between studies and translational progress.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 183-190"},"PeriodicalIF":3.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron-Quercetin complex enhances mesenchymal stem cell-mediated HGF secretion and c-Met activation to ameliorate acute kidney injury through the prevention of tubular cell apoptosis","authors":"Yuan-Xia Zou ,&nbsp;Jiraporn Kantapan ,&nbsp;Hong-Lian Wang ,&nbsp;Jian-Chun Li ,&nbsp;Hong-Wei Su ,&nbsp;Jian Dai ,&nbsp;Nathupakorn Dechsupa ,&nbsp;Li Wang","doi":"10.1016/j.reth.2024.12.003","DOIUrl":"10.1016/j.reth.2024.12.003","url":null,"abstract":"<div><h3>Background</h3><div>Acute kidney injury (AKI) is a life-threatening clinical syndrome with no effective treatment currently available. This study aims to investigate whether Iron-Quercetin complex (IronQ) pretreatment can enhance the therapeutic efficacy of Mesenchymal stem cells (MSCs) in AKI and explore the underlying mechanisms.</div></div><div><h3>Methods</h3><div>A cisplatin-induced AKI model was established in male C57BL/6 mice, followed by the intravenous administration of 1x10ˆ6 MSCs or IronQ-pretreated MSCs (MSC^IronQ). Renal function, histology, and tubular cell apoptosis were analyzed three days post-treatment. In vitro, apoptosis was induced in mouse tubular epithelial cells (mTECs) using cisplatin, followed by treatment with MSCs or MSC^IronQ conditioned medium (CM). Apoptosis was evaluated using TUNEL assay, RT-PCR, and western blotting. Furthermore, RNA sequencing (RNA-seq) was performed on MSC^IronQ to explore the underlying mechanisms.</div></div><div><h3>Results</h3><div>Compared to MSC-treated AKI mice, those treated with MSC^IronQ showed significantly improved renal function and histological outcomes, with reduced tubular cell apoptosis. A similar effect was observed in cisplatin-treated mTECs exposed to MSC^IronQ-CM. Mechanistically, RNA-seq and subsequent validation revealed that IronQ treatment markedly upregulated the expression and secretion of hepatocyte growth factor (HGF) in MSCs. Furthermore, RNA interference or antibody-mediated neutralization of HGF effectively abolished the anti-apoptotic effects of MSC^IronQ on mTECs. This mechanistic insight was reinforced by pharmacological inhibition of c-Met, the specific receptor of HGF, in both in vitro and in vivo models.</div></div><div><h3>Conclusions</h3><div>IronQ pretreatment enhances MSCs efficacy in AKI by promoting HGF expression and secretion, activating the HGF/c-Met pathway to suppress tubular cell apoptosis. These findings indicate that IronQ improves MSC-based therapies and offers insights into molecular mechanisms, supporting the development of better AKI treatments.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 169-182"},"PeriodicalIF":3.4,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of SFRP1 in human dermal papilla cell growth and its potential molecular mechanisms as a target in regenerative therapy","authors":"Chaofan Wang ,&nbsp;Yimei Du ,&nbsp;Changpei Lu ,&nbsp;Lingbo Bi ,&nbsp;Yunbu Ding ,&nbsp;Weixin Fan","doi":"10.1016/j.reth.2024.12.001","DOIUrl":"10.1016/j.reth.2024.12.001","url":null,"abstract":"<div><h3>Background</h3><div>Secreted frizzled-related protein 1 (SFRP1) inhibits Wnt signaling and is differentially expressed in human hair dermal papilla cells (DPCs). However, the specific effect of SFRP1 on cell function remains unclear. Telomerase reverse transcriptase (TERT) representing telomerase activity was found highly active around the hair dermal papilla. TERT levels can be enhanced by activation of the Wnt pathway in cancer cells and embryonic stem cells. Whether this regulatory mechanism is still present in DPCs has not been studied so far.</div></div><div><h3>Methods</h3><div>In this study, DNA plasmids and siRNAs were constructed against the SFRP1 gene and transfected into DPCs cultured in vitro. We detected the viability, proliferation, and migration of DPCs by Calcein/PI fluorescence, CCK-8, <em>trans</em>-well, or cell scratch experiments, and the expression of potential target genes was also determined through quantitative detection of RNA and protein.</div></div><div><h3>Results</h3><div>The results demonstrate a significant difference in SFRP1 levels from the control group, suggesting successful transfection of the DNA plasmid and siRNA of SFRP1 into IDPCs. Also, SFRP1 regulates the cell proliferation capacity of IDPCs and reduces their migration functions. The DPCs' living activity, proliferation, and migration function exhibited a negative correlation with the level of SFRP1. SFPR1 also inhibits the protein or RNA expression of β-catenin and TERT in DPCs.</div></div><div><h3>Conclusion</h3><div>It was proven that in human DPCs, different levels of SFRP1 change how cells work and control Wnt/β-catenin signaling or telomerase activity. This means that blocking SFRP1 could become a new way to treat hair loss diseases in the future.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 161-168"},"PeriodicalIF":3.4,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11718413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioengineering strategies for regeneration of skin integrity: A literature review","authors":"Makoto Shiraishi ,&nbsp;Yoshihiro Sowa ,&nbsp;Ataru Sunaga ,&nbsp;Kenta Yamamoto ,&nbsp;Mutsumi Okazaki","doi":"10.1016/j.reth.2024.12.006","DOIUrl":"10.1016/j.reth.2024.12.006","url":null,"abstract":"<div><h3>Objective</h3><div>The skin is a complex organ that includes various stem cell populations. Current approaches for non-healing skin defects are sometimes inadequate and many attempts have been made to regenerate skin integrity. The aim of this review is to bridge the gap between basic research and clinical application of skin integrity regeneration.</div></div><div><h3>Methods</h3><div>A literature search was carried out in PubMed using combinations of the keywords “skin integrity”, “tissue-engineered skin”, “bioengineered skin”, and “skin regeneration”. Articles published from 1968 to 2023 reporting evidence from <em>in vivo</em> and <em>in vitro</em> skin regeneration experiments were included.</div></div><div><h3>Results</h3><div>These articles showed that stem cells can be differentiated into normal skin cells, including keratinocytes, and are a significant source of skin organoids, which are useful for investigating skin biology; and that emerging direct reprogramming methods have great potential to regenerate skin from the wounded skin surface.</div></div><div><h3>Conclusion</h3><div>Recent advances in skin regeneration will facilitate further advancement of both basic and clinical research in skin biology.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"28 ","pages":"Pages 153-160"},"PeriodicalIF":3.4,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11713503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142954065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}