Prostaglandins, leukotrienes, and essential fatty acids最新文献_第6页

Actions of transforming growth factors on amnion cell prostaglandin biosynthesis. 转化生长因子对羊膜细胞前列腺素生物合成的影响。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-09-01

M D Mitchell

引用次数: 0

Determination of leukotriene B4, 6-keto-prostaglandin F1 alpha and thromboxane B2 in peritoneal lavage fluid of sham-operated and adrenalectomized rats. 假手术和去肾上腺大鼠腹腔灌洗液中白三烯B4、6-酮-前列腺素F1 α和血栓素B2的测定。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-09-01

J E Vincent, F J Zijlstra

引用次数: 0

Stimulation of prostaglandin E2 biosynthesis by estradiol in rat kidneys. 雌二醇对大鼠肾脏前列腺素E2生物合成的刺激。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-09-01

W C Chang

引用次数: 0

Radiation protection of the murine intestine by misoprostol, a prostaglandin E1 analogue, given alone or with WR-2721, is stereospecific. 米索前列醇(一种前列腺素E1类似物)单独或与WR-2721联合使用对小鼠肠道的辐射保护具有立体特异性。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

W R Hanson, K A Houseman, A K Nelson, P W Collins

{"title":"Radiation protection of the murine intestine by misoprostol, a prostaglandin E1 analogue, given alone or with WR-2721, is stereospecific.","authors":"W R Hanson, K A Houseman, A K Nelson, P W Collins","doi":"","DOIUrl":"","url":null,"abstract":"Misoprostol, a prostaglandin (PG) E1 analogue, is one of the most effective radiation protectors of the PGs investigated to date. Misoprostol-induced protection is also additive to protection by the widely studied thiol compound, WR-2721. The mechanism of PG-induced radiation protection and its interaction with WR-2721 is unknown. One important step in the investigation of the mechanism is to determine if PG-induced protection and its interaction with WR-2721 is mediated through PG receptor sites. A direct determination of receptor sites on murine intestinal clonogenic cells could not be made; however an indirect approach was possible. Misoprostol is composed of four stereoisomers of about equal proportions of which only one is gastric antisecretory and cytoprotective. Studies reported here compared radiation protection by this active isomer with that of one of the three inactive isomers. Furthermore, the additional protection of the two isomers when administered with WR-2721 was investigated. Results showed that only the active isomer was protective from radiation injury and this isomer was the only one which afforded additional protection with WR-2721. These data show that PG-induced radiation protection is receptor site dependent and stereospecific.","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"32 3","pages":"101-5"},"PeriodicalIF":3.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13979933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of leukotriene B4 on permeability, prostacyclin and thromboxane release by normal and oxygen-preexposed isolated, perfused rat lungs. 白三烯B4对正常和氧预暴露离体灌注大鼠肺通透性、前列环素和凝血素释放的影响。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

R M Jackson, H S Ann

{"title":"Effects of leukotriene B4 on permeability, prostacyclin and thromboxane release by normal and oxygen-preexposed isolated, perfused rat lungs.","authors":"R M Jackson, H S Ann","doi":"","DOIUrl":"","url":null,"abstract":"This study assessed the hemodynamic and permeability effects of exogenous, synthetic leukotriene B4 (LTB4) on normal rat lungs and lungs from rats preexposed to oxygen for 48 h, which were isolated and perfused at constant flow in vitro. Adult, Sprague-Dawley rats were exposed to air or greater than 97% O2 for 48 h. After exposure, their lungs were removed from the thorax, ventilated with normoxic gas, and perfused at 12 ml/min with Krebs-Ringer bicarbonate buffer which contained 5 mM glucose and 3 mg/ml albumin. A total of 5.55 micrograms of synthetic LTB4 was infused in three separate boluses over 15 minutes. Perfusion and airway pressures were monitored, and the lungs release of 6-ketoprostaglandin F1 alpha and thromboxane B2 (TXB2) into the effluent from the pulmonary vasculature was measured by radioimmunoassay. The LTB4 had no measureable effects on pulmonary vascular pressures. LTB4 infusion caused a pronounced increase in permeability, indicated by increased albumin concentrations in alveolar lavage fluid from O2-preexposed lungs. Release of TXB2 from both air- and O2-preexposed lungs was increased after LTB4 infusion, while the change in 6-ketoprostaglandin F1 alpha release was not statistically significant. Both the increase in permeability enhanced TXB2 released after LTB4 infusion were inhibited by 10 microM indomethacin in the perfusate. These data indicate that exogenous LTB4 increases microvascular permeability in O2-exposed lungs in association with increased release of TXB2 into the pulmonary vascular effluent.","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"32 3","pages":"121-8"},"PeriodicalIF":3.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13980509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of sulfidopeptide leukotrienes and their metabolism in human synovial fluid of patients with rheumatoid arthritis. 类风湿关节炎患者滑液中硫肽类白三烯的测定及其代谢。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

Y Koshihara, T Isono, H Oda, S Karube, Y Hayashi

{"title":"Measurement of sulfidopeptide leukotrienes and their metabolism in human synovial fluid of patients with rheumatoid arthritis.","authors":"Y Koshihara, T Isono, H Oda, S Karube, Y Hayashi","doi":"","DOIUrl":"","url":null,"abstract":"Leukotriene (LT)C4 in the synovial fluid of patients with osteoarthritis deformans (OA) and rheumatoid arthritis (RA) was measured by radioimmunoassay (RIA) after extraction with Sep-Pak C18 cartridge. The amounts of immunoreactive LTC4 (i-LTC4) in samples from patients with OA and RA were not significantly different, being 0.198 +/- 0.018 pmol/ml (n = 11) and 0.179 +/- 0.016 pmol/ml (n = 12), respectively. After separation by high performance liquid chromatography (HPLC) and measurement by RIA, the levels of other sulfidopeptide LTs, such as LTD4 and LTE4, in synovial fluid from patients with RA were found to be significantly higher than those in fluid from patients with OA. The leukocyte number in synovial fluids did not correlate with the i-LTC4 level. The metabolic activities of these synovial fluids were determined by incubating them with 3H-LTC4 and then separating sulfidopeptide LTs by HPLC. The conversion of LTC4 to LTD4 in synovial fluids of patients with OA and RA were similar, but the dipeptidase activity converting LTD4 to LTE4 was higher in fluid from patients with RA. It is suggested that a high level of LTE4 may contribute to exudation of synovial fluid, since LTE4 increases vascular permeability.","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"32 3","pages":"113-9"},"PeriodicalIF":3.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13979934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse embryo fibroblasts in culture: characteristics of arachidonic acid metabolism during early passages. 培养小鼠胚胎成纤维细胞:早期传代花生四烯酸代谢特征。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

S Durant, D Duval, F Homo-Delarche

{"title":"Mouse embryo fibroblasts in culture: characteristics of arachidonic acid metabolism during early passages.","authors":"S Durant, D Duval, F Homo-Delarche","doi":"","DOIUrl":"","url":null,"abstract":"In a model of mouse embryo fibroblasts in culture, which is characterized by a very rapid decrease in rate of cell proliferation during early passages, we determined the production of prostanoids either by following the transformation of the radioactive precursor [14C]-arachidonic acid or by radioimmunoassays. Our results demonstrate that mouse embryo fibroblasts in culture produce spontaneously substantial amounts of PGE2 and 6ketoPGF1 alpha as well as trace amounts of PGF2 alpha and of lipoxygenase derivatives (Hetes). We investigated the stability of the different types of prostaglandins produced. We showed that 6ketoPGF1 alpha and PGF2 alpha were stable in vitro, whereas PGE2 as a consequence of its solubilization in an aqueous medium was metabolized by 50% over a 24 h period, independently of the presence of the cell, thus leading to a constant underestimation of real PGE2 concentration. However, comparison of the patterns of prostaglandin production among subcultures of different orders was possible, and showed that the total amount of prostaglandin produced as well as the relative proportions are fairly identical during the first three passages, although the cell proliferation pattern rapidly decreases among the serial subcultures. These results suggest that prostaglandin production does not represent in our experimental model an autocrine means of regulating cell growth.","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"32 3","pages":"129-37"},"PeriodicalIF":3.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14267986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The urinary excretion of prostaglandins and thromboxane B2 in healthy volunteers: a study in males and females, and on the influence of seminal fluid contamination. 健康志愿者尿中前列腺素和血栓素B2的排泄:一项男性和女性的研究，以及精液污染的影响。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

P P Koopmans, C M Thomas, R J van de Berg, T Thien, F W Gribnau

引用次数: 0

Prostacyclin does not change during an oxygen induced increase in pulmonary blood flow in the fetal lamb. 在氧诱导的胎羊肺血流增加过程中，前列环素不发生变化。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

F C Morin, E A Egan, C E Lundgren, D D Swartz

{"title":"Prostacyclin does not change during an oxygen induced increase in pulmonary blood flow in the fetal lamb.","authors":"F C Morin, E A Egan, C E Lundgren, D D Swartz","doi":"","DOIUrl":"","url":null,"abstract":"The role of prostacyclin in mediating the increase in pulmonary blood flow caused by an increase in oxygen tension in the fetal lamb was investigated. Plasma concentrations of 6-keto-PGF1 alpha, the hydrolysis product of prostacyclin, were measured during an increase in pulmonary blood flow caused by a rise in oxygen tension in eight intrauterine fetal lambs. Fetal oxygen tension was increased by placing the pregnant ewes in a hyperbaric chamber and having them breathe 100% oxygen at three atmospheres absolute pressure. This increased fetal PaO2 from 27 +/- 3 to 60 +/- 6 torr (mean +/- S.E., p less than or equal to 0.0001) and increased the proportion of right ventricular output distributed to the fetal lungs from 6 +/- 2 to 45 +/- 7% (mean +/- S.E., p less than or equal to 0.001). However, the fetal plasma concentration of 6-keto-PGF1 alpha did not change, 186 +/- 26 to 208 +/- 40 pg/ml (mean +/- S.E.). Indomethacin decreased plasma concentrations of 6-keto-PGF1 alpha in each of three fetuses but did not decrease the proportion of right ventricular output distributed to their lungs. The increase in pulmonary blood flow caused by an increase in oxygen tension in the fetal lamb is not associated with an increase in plasma concentrations of 6-keto-PGF1 alpha. Prostacyclin does not appear to be involved in the increase in pulmonary blood flow caused by the increase in oxygen tension at birth.","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"32 3","pages":"139-44"},"PeriodicalIF":3.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14178231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Failure of non-selective inhibition of arachidonic acid metabolism to ameliorate hyperoxic lung injury. 非选择性抑制花生四烯酸代谢以改善高氧肺损伤的失败。

IF 3 4区医学

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-06-01

J R Hageman, J Zemaitis, R B Holtzman, S E Lee, L J Smith, C E Hunt

{"title":"Failure of non-selective inhibition of arachidonic acid metabolism to ameliorate hyperoxic lung injury.","authors":"J R Hageman, J Zemaitis, R B Holtzman, S E Lee, L J Smith, C E Hunt","doi":"","DOIUrl":"","url":null,"abstract":"We have previously reported that bronchoalveolar lavage fluid cyclo-oxygenase products of arachidonic acid (AA) metabolism increase prior to the development of significant hyperoxic lung injury. To further assess the role of AA metabolites in the development of hyperoxic lung injury, we have utilized this same model of hyperoxic lung injury and administered either indomethacin (an inhibitor of the cyclo-oxygenase pathway of AA metabolism) or dexamethasone (inhibitor of AA release). A total of 46 adult rabbits were exposed to greater than 95% oxygen for 65 hours. Fourteen animals were given either 2 or 3 mg/kg/day indomethacin, 7 served as controls: 18 animals were given either 0.5 or 1.0 mg/kg/day of dexamethasone, 7 served as controls. The surviving animals were sacrificed after 65 hours of hyperoxia and bronchoalveolar lavage of the left lung was done; the right lung was examined by light microscopy. Treatment with indomethacin or dexamethasone failed to ameliorate the hyperoxic lung injury process. However, in both the indomethacin and dexamethasone treatment groups, significant suppression of 6-keto-PGF1 alpha, a PGI2 metabolite, was observed. Some suppression of TXB2 production was observed, but there was no evidence of any decrease in leukotriene production. We postulate that failure to ameliorate hyperoxic lung injury with either indomethacin or dexamethasone therapy was related to significant suppression of PGI2, a potentially protective AA metabolite, and/or to failure to significantly decrease production of potential pathogenic participants, such as TXA2 or LTB4.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"32 3","pages":"145-53"},"PeriodicalIF":3.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14268682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0