Stimulation of prostaglandin E2 biosynthesis by estradiol in rat kidneys.

IF 2.9 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1988-09-01

W C Chang

引用次数: 0

Abstract

The effect of estradiol administration on renal prostaglandin (PG) E2 biosynthetic activity in rats was studied. A specific radioimmunoassay for PGE2 was developed and applied in the quantitation of PGE2 biosynthesis in kidney. Conversion of exogenous arachidonic acid into PGE2 by renal microsomal fraction was assayed. Formation of PGE2 was linear in fashion up to 5 min incubation at 37 degrees C, and linear in fashion up to 3.5 mg of microsome used as enzyme source. The renal biosynthesis of PGE2 was significantly increased by estradiol treatment.

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雌二醇对大鼠肾脏前列腺素E2生物合成的刺激。

研究了雌二醇对大鼠肾前列腺素E2生物合成活性的影响。建立了一种特异的PGE2放射免疫测定方法，并应用于肾脏中PGE2生物合成的定量测定。测定外源性花生四烯酸经肾微粒体部分转化为PGE2的情况。PGE2的形成在37℃下的5分钟内呈线性，在3.5 mg的微粒体中呈线性。雌二醇治疗可显著提高PGE2的肾脏生物合成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Prostaglandins, leukotrienes, and essential fatty acids 医学-内分泌学与代谢

CiteScore

6.40

自引率

6.70%

发文量

审稿时长

13.2 weeks

期刊介绍： The role of lipids, including essential fatty acids and their prostaglandin, leukotriene and other derivatives, is now evident in almost all areas of biomedical science. Cell membrane behaviour and cell signalling in all tissues are highly dependent on the lipid constituents of cells. Prostaglandins, Leukotrienes & Essential Fatty Acids aims to cover all aspects of the roles of lipids in cellular, organ and whole organism function, and places a particular emphasis on human studies. Papers concerning all medical specialties are published. Much of the material is particularly relevant to the development of novel treatments for disease.