Proceedings of the National Academy of Sciences of Belarus, Biological Series最新文献_第7页

Ecological functions of plant-microbial symbioses and their role in the development of resource-saving biotechnologies 植物-微生物共生的生态功能及其在资源节约型生物技术发展中的作用

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-05 DOI: 10.29235/1029-8940-2022-67-2-243-256

Ya. K. Kulikou

引用次数: 0

Molecular-genetic structure of Pseudomonas phage BIM BV-45 D 假单胞菌噬菌体BIM bv - 45d的分子遗传结构

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-190-196

T. A. Pilipchuk, A. Akhremchuk, E. I. Kаlаmiуets

{"title":"Molecular-genetic structure of Pseudomonas phage BIM BV-45 D","authors":"T. A. Pilipchuk, A. Akhremchuk, E. I. Kаlаmiуets","doi":"10.29235/1029-8940-2022-67-2-190-196","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-190-196","url":null,"abstract":"Full nucleotide sequence of bacteriophage Pseudomonas phage BIM BV-45 D – active component of biopesticide Multiphage intended for control of crop bacterial diseases caused by Pseudomonas syringae was analyzed. It was found that phage genome is represented by linear double-stranded DNA sized 40383 b. p. (average GC contents equals 58 %), comprising 46 open reading frames, including 13 described in genomes of closely related phages. 4 control sequences typical for bacterial genes recognized by sigma factor (σ70) of RNA polymerase were detected, specific phage promoters were not localized. The identity was established of the majority of protein amino acid sequences of the Pseudomonas phage BIM BV-45 D with the proteins of the known Pseudomonas phage Andromeda (95‒100 %), at the same time, the sequences of the DNA endonuclease protein (gene 22) are similar (63 %) to the corresponding protein of Pseudomonas phage PollyC. The obtained data suggest that the mosaic structure of Pseudomonas phage BIM BV-45 D genome is due to recombinant rearrangements between the afore-mentioned phages.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91112662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ecological adaptability of blueberry cultivars introduced in Belarus 白俄罗斯引种蓝莓品种生态适应性评价

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-206-218

N. Pavlovskiy

引用次数: 0

Amino acid balance of microbial-tissue complex in the small intestinal and liver under administration of lead acetate 醋酸铅对小肠和肝脏微生物组织复合物氨基酸平衡的影响

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-197-205

I. V. Nikalayeva, V. Sheibak, E. Doroshenko

{"title":"Amino acid balance of microbial-tissue complex in the small intestinal and liver under administration of lead acetate","authors":"I. V. Nikalayeva, V. Sheibak, E. Doroshenko","doi":"10.29235/1029-8940-2022-67-2-197-205","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-197-205","url":null,"abstract":"It is known that the initiating event of liver damage during lead intoxication is the effect of lead on the intestinal microbiome and the metabolic profile of enterocytes. The aim of the study was to reveal the dependence of the concentrations of free amino acids and their derivatives in the liver on the amino acid pool of the microbial-tissue complex of the small intestine. We used rats weighing 120‒140 g, which were injected intragastrically for three weeks with drinking water with lead acetate. Free amino acids and their nitrogen-containing metabolites in the microbial-tissue complex of the intestine and liver were determined by HPLC. Enteral intake of lead acetate with drinking water changes the profile of free amino acids and their derivatives in the microbial-tissue complex of the small intestine, which correlates with a violation of amino acid homeostasis in the liver. The analysis of the correlations of the indicators we determined indicates the essential role of nitrogen-containing metabolites of amino acids ‒ ethanolamine and phosphoethanolamine, taurine, and cystathionine. The direction of the correlation relationship between various nitrogen-containing metabolites of the microbial-tissue complex and the liver can be considered as marker of discoordination of homeostasis.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87327686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and characteristics of Ashworthius sidemi Schulz, 1933 (Nematoda, Trichostrongylidae) in wild ungulates in Belarus 白俄罗斯野生有蹄类动物中线虫的分布和特征

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-172-180

P. Y. Lobanovskaya, L. Akimova, E. Kheidorova

引用次数: 0

Molecular-genetic characteristics of patients with diabetes mellitus. Vestsi Natsyyanal’nai akademii navuk Belarusi 糖尿病患者的分子遗传学特征。Vestsi Natsyyanal 'nai akademii navuk白俄罗斯

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-158-171

M. Lushchyk, M. Ameliyanovich, H. Tuzava, I. Mosse, L. Danilova

{"title":"Molecular-genetic characteristics of patients with diabetes mellitus. Vestsi Natsyyanal’nai akademii navuk Belarusi","authors":"M. Lushchyk, M. Ameliyanovich, H. Tuzava, I. Mosse, L. Danilova","doi":"10.29235/1029-8940-2022-67-2-158-171","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-158-171","url":null,"abstract":"The article discusses the prospects for studying polymorphic variants of peroxisome proliferator-activated receptor genes (PPARs) of three types (PPARα, PPARδ, and PPARγ) in diabetes mellitus (DM), taking into account their key role in the regulation of energy homeostasis, production of pro-inflammatory cytokines, and lipid characteristics and glycemia control. The main emphasis is on the use of screening methods for testing patients for carriage of single nucleotide polymorphisms (SNPs) in order to improve approaches to identifying risk groups for the formation of DM and associated diseases, and subsequent personification of corrective measures. The clinical, laboratory and molecular genetic characteristics of groups of patients with type 1 and 2 diabetes, healthy volunteers are presented. The prevalence of SNPs in the genes of receptors activated by the peroxisome proliferator in patients with DM was studied in comparison with the control group. Among the evaluated SNPs of the rs135551 gene, PPARA showed the clearest association with the presence of DM. Four variants of haplotypes highly associated with DM1 and DM2 were identified. The expediency of further clarification of the clinical and genetic heterogeneity of cases of diabetes within the DM1 and DM2 groups is discussed. The prospects of this direction for the development of preventive technologies in diabetology, long-term epidemiological molecular genetic screenings are assessed.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79133099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of 5-aminolevulinic acid on physiological and biochemical characteristics of winter wheat varieties with different levels of anthocyanins in coleoptiles 5-氨基乙酰丙酸对芽鞘花青素含量不同冬小麦品种生理生化特性的影响

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-135-146

N. G. Averina, S. M. Savina, I. Dremuk, H. Yemelyanava, Yu. V. Pryshchepchyk, А. V. Usatov

{"title":"Influence of 5-aminolevulinic acid on physiological and biochemical characteristics of winter wheat varieties with different levels of anthocyanins in coleoptiles","authors":"N. G. Averina, S. M. Savina, I. Dremuk, H. Yemelyanava, Yu. V. Pryshchepchyk, А. V. Usatov","doi":"10.29235/1029-8940-2022-67-2-135-146","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-135-146","url":null,"abstract":"Physiological and biochemical characteristics of two varieties of winter wheat seedlings with red (Et W5) and green (Vladi) coleoptiles have been studied. Variety Et W5 with high content of anthocyanins (on average 412 ± 52 μmol/g fr wt), chlorophylls (Chl) and carotenoids and a low level of heme and proline in coleoptiles had high frost resistance (88 % of surviving plants exposed to temperatures of ‒8 °C for 5 h at the age of 7 days) and a high ability to continue growth and development after undergoing cooling. Variety Vladi with a low content of anthocyanins (on average 12.5 ± 1.2 μmol/g fr wt), Chl and carotenoids, but a higher level of heme and proline in coleoptiles compared to varieties Et W5 had low level of frost resistance (80 % of surviving plants) and was characterized by a low ability to continue growth and development after undergoing cooling. Soaking wheat seeds in a solution of exogenous ALA (50 mg/l) for 2 h before sowing increased the germination of seeds of both varieties, enhanced the length of plants of the Et W5 variety by 10 %, 1.4 times the content of anthocyanins in the coleoptiles of this variety plants and also increased their frost resistance up to 97 %. Under action of ALA frost resistance of Vladi seedlings increased up to 93 %.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81773735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of epibrassinolide and its sulfuric acid esters on the morphometric parameters of sunflower Helianthus annuus L. (cv. Helios) 表油菜素内酯及其硫酸酯对向日葵形态计量学参数的影响。赫利俄斯)

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-181-189

Ya. V. Khamiuk, R. Litvinovskaya, A. Kolbas

引用次数: 0

Flow cytometry. Basics of technology and its application in biology 流式细胞术。技术基础及其在生物学中的应用

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-229-242

I. Volotovski, S. Pinchuk

引用次数: 0

Highbush blueberry (Vaccinium corymbosum L.): proteomic and molecular genetic studies. Vestsi Natsyyanal’nai akademii navuk Belarusi 高丛蓝莓(Vaccinium corymbosum L.):蛋白质组学和分子遗传学研究。Vestsi Natsyyanal 'nai akademii navuk白俄罗斯

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-147-157

O. V. Chizhik, A. N. Yukhimuk, V. Reshetnikov

{"title":"Highbush blueberry (Vaccinium corymbosum L.): proteomic and molecular genetic studies. Vestsi Natsyyanal’nai akademii navuk Belarusi","authors":"O. V. Chizhik, A. N. Yukhimuk, V. Reshetnikov","doi":"10.29235/1029-8940-2022-67-2-147-157","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-2-147-157","url":null,"abstract":"The Vacciniaceae family introduction carry out by the Central Botanical Garden of the National Academy of Sciences of Belarus since 1980. It made it possible to give rise the new direction for the republic – the industrial berry growing. To increase the efficiency of high-quality planting material obtaining it is necessary to study the fundamental processes underlying the growth and development of plants and to establish the possible ways of their regulation. It is of current interest to confirm the conformity of the planting material to the declared variety (the genetic passports elaboration). The biotechnology development promotes a new approaches and technologies for studying and analyzing of a plant organism genome and proteome. For the first time the molecular genetic identification for Vaccinium corymbosum L. varieties were carried out by Start Codon Targeted (SCoT) system. This method of DNA certification makes it possible to check the cultivar conformity of the blueberry planting material. For the first time by 2D electrophoresis the proteomic maps of leaf tissue were obtained and the proteomic status of four highbush blueberry varieties – in vitro culture and parental plants (in vivo) was estimated. The species and varieties specificity of the expressed genome products were established. The proteins that claims to be markers for the Vaccinium species and the variety markers were identified for the first time, as well as the physiological state markers of high blueberry cultivated in vivo and in vitro.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89799429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0