假单胞菌噬菌体BIM bv - 45d的分子遗传结构

T. A. Pilipchuk, A. Akhremchuk, E. I. Kаlаmiуets
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引用次数: 0

摘要

分析了用于防治丁香假单胞菌引起的作物细菌性病害的生物农药Multiphage活性成分——噬菌体Pseudomonas phage BIM bv - 45d的全核苷酸序列。结果发现,噬菌体基因组以40383 b.p .的线性双链DNA为代表(平均GC含量为58%),包括46个开放阅读框,其中13个在密切相关的噬菌体基因组中描述。检测到4个被RNA聚合酶sigma因子(σ70)识别的典型细菌基因的对照序列,未定位特异性噬菌体启动子。假单胞菌噬菌体BIM bv - 45d的大部分蛋白质氨基酸序列与已知的假单胞菌噬菌体Andromeda的蛋白质序列一致(95% ~ 100%),同时DNA内切酶蛋白(基因22)序列与假单胞菌噬菌体polyc的相应蛋白相似(63%)。所得数据表明假单胞菌噬菌体BIM bv - 45d基因组的镶嵌结构是由于上述噬菌体之间的重组重排所致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular-genetic structure of Pseudomonas phage BIM BV-45 D
Full nucleotide sequence of bacteriophage Pseudomonas phage BIM BV-45 D – active component of biopesticide Multiphage intended for control of crop bacterial diseases caused by Pseudomonas syringae was analyzed. It was found that phage genome is represented by linear double-stranded DNA sized 40383 b. p. (average GC contents equals 58 %), comprising 46 open reading frames, including 13 described in genomes of closely related phages. 4 control sequences typical for bacterial genes recognized by sigma factor (σ70) of RNA polymerase were detected, specific phage promoters were not localized. The identity was established of the majority of protein amino acid sequences of the Pseudomonas phage BIM BV-45 D with the proteins of the known Pseudomonas phage Andromeda (95‒100 %), at the same time, the sequences of the DNA endonuclease protein (gene 22) are similar (63 %) to the corresponding protein of Pseudomonas phage PollyC. The obtained data suggest that the mosaic structure of Pseudomonas phage BIM BV-45 D genome is due to recombinant rearrangements between the afore-mentioned phages.
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