Highbush blueberry (Vaccinium corymbosum L.): proteomic and molecular genetic studies. Vestsi Natsyyanal’nai akademii navuk Belarusi

The Vacciniaceae family introduction carry out by the Central Botanical Garden of the National Academy of Sciences of Belarus since 1980. It made it possible to give rise the new direction for the republic – the industrial berry growing. To increase the efficiency of high-quality planting material obtaining it is necessary to study the fundamental processes underlying the growth and development of plants and to establish the possible ways of their regulation. It is of current interest to confirm the conformity of the planting material to the declared variety (the genetic passports elaboration). The biotechnology development promotes a new approaches and technologies for studying and analyzing of a plant organism genome and proteome. For the first time the molecular genetic identification for Vaccinium corymbosum L. varieties were carried out by Start Codon Targeted (SCoT) system. This method of DNA certification makes it possible to check the cultivar conformity of the blueberry planting material. For the first time by 2D electrophoresis the proteomic maps of leaf tissue were obtained and the proteomic status of four highbush blueberry varieties – in vitro culture and parental plants (in vivo) was estimated. The species and varieties specificity of the expressed genome products were established. The proteins that claims to be markers for the Vaccinium species and the variety markers were identified for the first time, as well as the physiological state markers of high blueberry cultivated in vivo and in vitro.