Proceedings of the National Academy of Sciences of Belarus, Biological Series最新文献_第8页

Distribution of pBS72-like conjugative plasmids among natural bacteria of the genus Bacillus pbs72样结合质粒在芽孢杆菌属天然细菌中的分布

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-05-04 DOI: 10.29235/1029-8940-2022-67-2-219-228

A. Hurynovich, N. E. Satsunkevich, M. Titok

引用次数: 0

Carotenogenesis in Haematococcus pluvialis cells under the combined action of excess of NaCl in the culture medium and high light intensity 在培养基中过量NaCl和强光的共同作用下雨红球菌细胞中胡萝卜素的生成

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-66-74

T. V. Samovich, Y. V. Viazau, R. Goncharik, E. I. Pechenkina, N. Kozel

{"title":"Carotenogenesis in Haematococcus pluvialis cells under the combined action of excess of NaCl in the culture medium and high light intensity","authors":"T. V. Samovich, Y. V. Viazau, R. Goncharik, E. I. Pechenkina, N. Kozel","doi":"10.29235/1029-8940-2022-67-1-66-74","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-66-74","url":null,"abstract":"The parameters of growth and development of the microalga Haematococcus pluvialis, as well as the process of carotenogenesis, including the pigment composition and expression of the key genes of astaxanthin biosynthesis, were studied in algal cells under the combined action of excess of NaCl in the culture medium and high light intensity. It was found that the combination of excess of NaCl in the culture medium for Haematococcus pluvialis, initiated at the resting stage of the microalga, and high light intensity is not an effective method for induction of the accumulation of astaxanthin in the cells of Haematococcus pluvialis. A decrease in the level of expression of one of the key genes of carotenogenesis - phytoene synthase gene, was revealed under such conditions. At the same time, it was found that the combined action of high light intensity and excess of NaCl led to an increase in the ratio of astaxanthin cis-isomers, which are more biotechnologically valuable, in the total pigment pool.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88744867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and seasonal dynamics of diversity of spiders in the bilberry and mossy pine forests of the reserve “Pribuzhskoye Polesie” “Pribuzhskoye Polesie”保护区越橘和苔藓松林蜘蛛多样性的结构和季节动态

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-84-90

E. I. Fedosenko

引用次数: 0

Аge structure and fecundity of the noble crayfish populations in abandoned quarries Аge废弃采石场中高贵小龙虾种群的结构和繁殖力

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-75-83

A. Alekhnovich

{"title":"Аge structure and fecundity of the noble crayfish populations in abandoned quarries","authors":"A. Alekhnovich","doi":"10.29235/1029-8940-2022-67-1-75-83","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-75-83","url":null,"abstract":"The population of noble crayfish (Astacus astacus) was researched in quarries of an old brick plant in Molodechno district of Minsk region. Rare disappearing noble crayfish was found in 10 closed quarries. The presence of crayfish in closed quarries that are not connected with watercourses indicates that these populations were created by man. The population of noble crayfish in the quarries of an old brick factory is characterized by a high number. The average catch by eel traps was 1.31 ± 0.77 individuals/trap/day. Crayfish in the first and second year of life grew faster in quarries than in other habitats, but subsequently the average size of individuals in the quarry was smaller in comparison with the general model of noble crayfish growth in Belarus. Individuals that were older than 6 years have not been found in quarries. This indicates that there are factors that determine high mortality rate in older age groups. However, favorable conditions for the growth of juveniles allow the population to maintain their numbers at a high level. The reason for this phenomenon remains unknown. The size of noble crayfish individuals reaching puberty and the fertility is comparable to those from other habitats. In the quarry, the minimum length of an oviparous female was 7.2 cm. Egg-laying females in the 7.0-7.9 cm size class were 16.7 %, in the 8.0-8.9 cm size class - 38.8, in the 9.0-9.9 cm size class - 70.6 %, in the size class of 10.0-10.9 cm all the females were oviparous.The population of noble crayfish from the quarries of Molodechno district should be considered as donor population for carrying out resettlement into the water bodies of the river Neman.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84902418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of the spectral composition of light on the morphogenesis of the epiphytic orchid Cattleya labiata Lindl. cultivated in the greenhouse 光光谱组成对附生兰形态发生的影响。温室栽培

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-16-23

N. V. Hetko, N. A. Burchik, M. I. Barkun, V. Titok

{"title":"Influence of the spectral composition of light on the morphogenesis of the epiphytic orchid Cattleya labiata Lindl. cultivated in the greenhouse","authors":"N. V. Hetko, N. A. Burchik, M. I. Barkun, V. Titok","doi":"10.29235/1029-8940-2022-67-1-16-23","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-16-23","url":null,"abstract":"This work evaluates the test results of 3 types of LED lamps with different spectral composition of radiation and photon flux of photosynthesis (PPF, μmol/m2/sec): PPG T8I Agro (37), Navigator DSP-FITO (47.5) and DDP06-3×8-004 UHL 4 ‘Home Farm' (100), in the greenhouse culture of the orchid Cattleya labiata Lindl. The parameters of the thickness of annual leaves, the percentage of dry matter and the increase in biomass per unit of leaf surface (μg/cm2) formed over a period of 140 days were used as indicators to assess the degree of influence of the spectral composition of light on the morphogenesis of C. labiata plants. The spectral characteristics of the LED lamp DDP06-3×8-004 UHL 4 ‘Home Farm' with a white glow, intensity PPF 100 are considered as the most favorable for the successful greenhouse cultivation of the orchid Cattleya labiata Lindl. The combination in its spectrum of red and green spectra with their ratio equal to 1.3, and when the ratio of red and blue spectra equal to 3.34, a higher level of dry matter accumulation is observed per unit of leaf surface area white maintaining parameter of the leaf structure characteristic of a given plant species.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82171534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of Pseudomonas chlororaphis subsp. aurantiacа strains capable of phenasine compounds overproduction 绿假单胞菌亚种的蛋白质组学分析。能够过量生产phenasine化合物的aurantiac_2菌株

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-91-104

K. Verameyenka, M. A. Shapira, V. A. Naumouskaya, D. D. Ashmankevich, N. Maximova

{"title":"Proteomic analysis of Pseudomonas chlororaphis subsp. aurantiacа strains capable of phenasine compounds overproduction","authors":"K. Verameyenka, M. A. Shapira, V. A. Naumouskaya, D. D. Ashmankevich, N. Maximova","doi":"10.29235/1029-8940-2022-67-1-91-104","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-91-104","url":null,"abstract":"Proteomic analysis is a highly effective method for bacteria identification and the elucidation of protein's content in prokaryotic cells at different growth conditions. To our knowledge this approach is hardly ever used for characterization of producers of biologically active substances. The understanding of the changes in protein profile in mutant strains capable of biologically active substances overproduction helps to recognize the biochemical and molecular basis of metabolic changes which lead to overproduction. So that, proteomic analysis could be especially useful for optimization the producer's creation techniques.The purpose of current research was to carry out proteomic profiling of bacteria P. chlororaphis subsp. aurantiaca mutant strains capable of overproduction of phenazine antibiotics. Microbiological and biochemical methods were used for these aims.In current research a proteomic analysis of strains of P. chlororaphis subsp. aurantiaca producing phenazines was carried out. An early (during log-phase) onset of expression of individual genes of phz-operon which codes enzymes for phenazines synthesis was demonstrated. It was also found that the wild type strain has the highest level of PhzO protein. The gene encoding this protein is located outside the phz-operon. We weren't able to establish the correlation among PhzO protein content and concentration of the derivatives for which appearance PhzO is responsible. A general tendency of producer strains towards the accumulation of enzymes and proteins of the antioxidant defense system was revealed. Producer strains also demonstrate a significant increase in the concentration of proteins involved in DNA repair as well as chaperones involved in the native protein conformation maintenance.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75926972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of microorganism’s resistant to soil salinization 土壤耐盐碱化微生物的分离与鉴定

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-54-65

З. М. Алещенкова, И. Н. Ананьева, Идентификация Микроорганизмов, I. N. Ananyeva

{"title":"Isolation and identification of microorganism’s resistant to soil salinization","authors":"З. М. Алещенкова, И. Н. Ананьева, Идентификация Микроорганизмов, I. N. Ananyeva","doi":"10.29235/1029-8940-2022-67-1-54-65","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-54-65","url":null,"abstract":"315 variants of halotolerant bacteria were isolated from soil samples taken around Starobin potash deposit mined by Belaruskali concern. Nitrogen-fixing isolate CA-6 and phosphate-solubilizing isolate Cp-1 capable to withstand abiotic stress caused by sodium chloride in concentration 1710 and 2565 mM, respectively and water deficiency characterized by osmotic potential -0.20 and -0.42 MPa were sorted out. Isolate Cp-1 may dissolve phosphates yielding halo zones 9 mm in diameter while isolate CA-6 displays nitrogenase activity 44.0 nM C2H2/vial/3 days. Both isolates are growth-promoting organisms, distinguished by the ability to synthesize IAA in concentrations 89.7 and 37.8 μg IAA/ml of cultural liquid, respectively. The selected salt-resistant isolates were identified based on analysis of the nucleotide sequence of the 16S rRNA gene and data of MALDI-TOF mass spectrometry and were referred to the species Rhodococcus jostii and Priestia megaterium. Halotolerant strains Priestia megaterium Cp-1 and Rhodococcus jostii CA-6 were deposited in Belarusian collection of non-pathogenic microorganisms under the respective registration numbers BIM B-1314D and BIM B-1353D.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91092731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Three-dimensional 3D-bioprinting: the basis of technology and its application in the interests of biology and medicine 三维3d生物打印:技术基础及其在生物学和医学领域的应用

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-114-126

I. Volotovski, S. Pinchuk

{"title":"Three-dimensional 3D-bioprinting: the basis of technology and its application in the interests of biology and medicine","authors":"I. Volotovski, S. Pinchuk","doi":"10.29235/1029-8940-2022-67-1-114-126","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-114-126","url":null,"abstract":"This review is devoted to one of the actual problem of modern biology and medicine, we are witnessing. The text provides information on 3D printing method, equipment that is used to manufacture of three-dimensional bioproducts and the nature of the consumable materials - bioinks, fillers and cells which during printing transform into three-dimensional bioproduct characterized by vitality and expression of specific marker factors of corresponding tissues and organs. Special emphasis in this review is made on prospects of use of stem cells which are along with a variety of cells of tissues and organs have found application in bioprinting. Three methods of bioprinting: inkjet, extrusion and laser are described. Advantages and disadvantages of each method are discussed. Bioinks, their properties applicability to various printing technologies and strategy selection of bioink and cell composition defining viability of three-dimensional structures and possibility of their application in clinical praxis are detailly described. Short list of achievements in the field of bioprinting of various organism tissues and the characteristics of the bioproducts obtained are given. Significant progress and promising research in the field of 3D bioprinting are marked.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81818172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biologically active compounds of berries and leaves of Vaccinium corymbosum L. 金莲果和叶的生物活性成分研究。

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-7-15

O. V. Chizhik, A. M. Deeva, V. Reshetnikov

{"title":"Biologically active compounds of berries and leaves of Vaccinium corymbosum L.","authors":"O. V. Chizhik, A. M. Deeva, V. Reshetnikov","doi":"10.29235/1029-8940-2022-67-1-7-15","DOIUrl":"https://doi.org/10.29235/1029-8940-2022-67-1-7-15","url":null,"abstract":"Quantitative content of the sum of phenolic compounds and anthocyanins in their composition were study. The level of antiradical activity (ARA) in the berries and leaves of Vaccinium corymbosum L. was measured.The maximum content of the sum of phenolic compounds was observed in Blueray, Bluerose, Caroline Blue, Herbert, Jersey, Nelson cultivars. In the cultivars which have been studied the quantity of anthocyanins in the composition of phenolic compounds ranged from 0.01 to 0.8 % in green berries, and from 22 to 52 % in mature ones. Earlyblue, Duke, Bluecrop, Bluegold cultivars were characterized by the greatest quantity of phenolic compounds in leaves.The presence of a close positive correlation between the level of antiradical activity and the content of secondary metabolites of phenolic nature was determined. The differences in phenolic substances accumulation in blueberry cultivars obtained by in vivo and in vitro methods were revealed.It was shown that the proteomic profile of the highbush blueberry plants together with genetic passports can being used to determine the cultivar's identity of Vaccinium corymbosum L.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77287562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection criteria and characterization of sporulating bacterial strains of genus Bacillus – the basis of probiotic preparations and feed additives for livestock 芽孢杆菌属产孢菌株的选择标准和特性。家畜用益生菌制剂和饲料添加剂的基础

Proceedings of the National Academy of Sciences of Belarus, Biological Series Pub Date : 2022-02-02 DOI: 10.29235/1029-8940-2022-67-1-105-113

N. Sverchkova, E. Kolomiets

引用次数: 0