流式细胞术。技术基础及其在生物学中的应用

I. Volotovski, S. Pinchuk
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引用次数: 0

摘要

流式细胞术在细胞生物学、生物物理学、生物化学和分子生物学等生物学的各个领域以及应用医学和诊断医学中都是一种广泛应用的基础研究方法,本文试图对流式细胞术问题进行集中探讨。流式细胞仪的方法原理,结构及其可能性(细胞群结构和功能状态的研究和细胞分选),激光在流式细胞仪中的应用,各种荧光团和单克隆抗体。给出了流式细胞仪方法在不同实验中的具体实例。展望了流式细胞术的发展趋势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Flow cytometry. Basics of technology and its application in biology
The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different experiments are given. The trends in development of flow cytometry are considered.
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