Flow cytometry. Basics of technology and its application in biology

Abstract

The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different experiments are given. The trends in development of flow cytometry are considered.