1st Portuguese Biomedical Engineering Meeting最新文献_第5页

New platforms for the downstream processing of biopharmaceuticals 生物制药下游加工新平台

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026043

A. Azevedo, M. Aires-Barros

引用次数: 1

A cardiac signal monitoring and processing system 一种心脏信号监测与处理系统

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026046

V. Almeida, T. Pereira, H. Pereira, J. Cardoso, C. Correia

引用次数: 0

Production of biopolyhydroxyalkanoates 生物聚羟基烷酸酯的生产

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026078

Catarina Dias de Almeida, M. Cesário, M.M.R. da Fonseca

引用次数: 0

Membrane chromatography for therapeutic DNA production: Adsorption membranes development 用于治疗性DNA生产的膜层析:吸附膜的发展

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026054

M. Mateus, L. Raiado-Pereira, M. Prazeres

引用次数: 0

On the benefits of classical multidimensional scaling in Epileptic seizure prediction studies 经典多维标度在癫痫发作预测研究中的应用

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026063

B. Direito, C. Teixeira, A. Dourado

引用次数: 1

Effect of hypoxia on proliferation and neural commitment of embryonic stem cells at different stages of pluripotency 缺氧对胚胎干细胞多能性不同阶段增殖和神经功能的影响

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026039

Tiago G Fernandes, M. M. Diogo, A. Fernandes-Platzgummer, C. Lobato da Silva, J. Cabral

{"title":"Effect of hypoxia on proliferation and neural commitment of embryonic stem cells at different stages of pluripotency","authors":"Tiago G Fernandes, M. M. Diogo, A. Fernandes-Platzgummer, C. Lobato da Silva, J. Cabral","doi":"10.1109/ENBENG.2011.6026039","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026039","url":null,"abstract":"We have studied the effect of low oxygen levels (2% O2), or hypoxia, in the expansion and neural commitment of mouse embryonic stem (ES) cells. When ES cells were maintained in culture with leukemia inhibitory factor (LIF), cell proliferation was reduced at low oxygen levels and a simultaneous reduction in cell viability was also observed. Morphological changes and different cell cycle patterns also occurred, suggesting some early differentiation under hypoxic conditions. However, when cells were maintained in a ground state of pluripotency, by inhibition of autocrine FGF4/ERK and GSK3 signaling, hypoxia did not affect cell proliferation, and did not induce early differentiation. Nevertheless, during neural commitment, low oxygen tension exerted a positive effect on early differentiation of ES cells, resulting in a faster commitment towards neural progenitors. Overall our results demonstrate the need to specifically regulate the oxygen content, especially hypoxia, along with other culture conditions, when developing new strategies for ES cell expansion and/or controlled differentiation.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116949109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Automatic classification of cognitive states 认知状态的自动分类

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026089

C. Cabral, M. Silveira, P. Figueiredo

{"title":"Automatic classification of cognitive states","authors":"C. Cabral, M. Silveira, P. Figueiredo","doi":"10.1109/ENBENG.2011.6026089","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026089","url":null,"abstract":"Functional Magnetic Resonance Imaging has established itself as the most powerful technique available today to measure brain activity induced by a perceptual or cognitive state. The inverse problem is considered in this study; given the measured brain activity, our goal is to predict the perceptual state. Machine Learning algorithms were used to address this problem in this work. Multi-subject fMRI data analysis poses a great challenge for the machine learning paradigm, by its characteristics: the low Signal to Noise Ratio (SNR), high dimensionality, small number of examples and inter-subject variability. To address this problem, several methods of classification and feature selection were tested. The main criterion of feature selection was mutual information in a univariate method, but a multivariate feature selection was also proposed. Both a single classifier and an ensemble of classifiers were tested. The ensemble of classifiers approach consisted on training an optimized classifier for each class and then the combination was made. The data analysed was obtained from three multi-subject experiments of visual stimulation with 4 classes of stimuli, at different magnetic field strengths. The ensemble of classifiers performs best for most data sets and methods of feature selection. In summary, the results suggest that a combination of classifiers can perform better than a single classifier, particularly when decoding stimuli associated with specific brain areas.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129134920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic separation of undifferentiated mouse Embryonic Stem (ES) cells from neural progenitor cultures using a microfluidic device 利用微流体装置磁分离未分化小鼠胚胎干(ES)细胞与神经祖细胞的培养

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026049

A. F. Sousa, J. Loureiro, M. M. Diogo, J. Cabral, P. Freitas

引用次数: 0

In vitro evaluation of osteoconductive starch based scaffolds under dynamic conditions 动态条件下骨导电性淀粉基支架的体外评价

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026083

Ana I. Rodrigues, M. Gomes, I. Leonor, R. Reis

{"title":"In vitro evaluation of osteoconductive starch based scaffolds under dynamic conditions","authors":"Ana I. Rodrigues, M. Gomes, I. Leonor, R. Reis","doi":"10.1109/ENBENG.2011.6026083","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026083","url":null,"abstract":"The purpose of this research work is the in vitro evaluation of the osteogenic potential of fiber meshes scaffolds based on a blend of corn starch with poly-caprolactone with Si-OH groups using a flow perfusion bioreactor. Scaffolds based on a blend of corn starch with poly-caprolactone with Si-OH groups were seeded with human adipose stem cells and cultured in basal or osteogenic medium, under static and dynamic conditions for 7, 14 and 21 days. Starch with poly-caprolactone scaffolds were analysed in the same conditions and used as controls. The DNA quantification assay showed a high proliferation rate of human adipose stem cells cultured on scaffolds of of corn starch with poly-caprolactone with Si-OH groups in osteogenic medium under dynamic conditions. Furthermore, scanning electron microscopy images showed that cells penetrated into the pores of the scaffold and demonstrated the existence of some mineralization nodules with significant Calcium and Phosphorous levels as quantified by energy dispersive spectroscopy analysis. Osteogenic differentiation of human adipose stem cells was also confirmed by positive Alizarin Red staining in cell cultured scaffolds of corn starch with poly-caprolactone with Si-OH groups and by relevant ALP activity.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122886814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Response and resistance to drugs and chemical stress in the yeast model: A genome-wide view 酵母模型对药物和化学胁迫的反应和抗性:全基因组视角

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026076

S. D. dos Santos, Miguel C. Teixeira, N. Mira, T. R. Cabrito, M. Palma, Artur B. Lourenço, P. Dias, J. Guerreiro, A. Moreira, Sílvia F. Henriques, I. Sá-Correia

引用次数: 0