1st Portuguese Biomedical Engineering Meeting最新文献_第6页

Development of an electroactive textil system for the objective assessment of sleep movements and neurodegenerative diseases 用于客观评估睡眠运动和神经退行性疾病的电活性纺织系统的开发

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026072

M. Tavares, N. Belino, M. V. Patto, M. J. Geraldes

引用次数: 2

Electromagnetic tomography: Real-time imaging using linear approaches 电磁断层成像:使用线性方法的实时成像

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026096

J. Caeiros, R. C. Martins

{"title":"Electromagnetic tomography: Real-time imaging using linear approaches","authors":"J. Caeiros, R. C. Martins","doi":"10.1109/ENBENG.2011.6026096","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026096","url":null,"abstract":"The main objective of the present work consists in the development of linear image reconstruction algorithms for two tomographic technologies aimed for biomedical applications, the Magnetic Induction Tomography (MIT) and the Electrical Impedance Tomography (EIT). This required the implementation of numerical forward problem solvers, which simulate the interaction between a given electromagnetic field and a body with a specific conductivity distribution. A new subgridding scheme was developed for the MIT forward problem. Both solvers are fully automatic, requiring only the specification of the problem data and allow the obtainment of virtual measurements that can be used in the image reconstruction process. Regarding the inverse problems, different reconstruction algorithms were studied in order to get images in real-time. The Back-Projection and the Filtered Back-Projection methods were implemented, either along straight lines, or along magnetic flux lines (specifically for the MIT) or equipotential lines (specifically for EIT). The state of the art image reconstruction algorithm GREIT was also implemented and applied for the first time to the resolution of the 2D EIT and 3D MIT inverse problems. The results obtained were satisfying since they demonstrate the applicability of these technologies to the clinical environment.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126631066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Using systems biology approaches to study a multidrug resistance network 利用系统生物学方法研究多药耐药网络

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026073

P. Dias, Catarina Costa, I. Sá-Correia, Miguel C. Teixeira, Pedro T. Monteiro, Arlindo L. Oliveira, A. T. Freitas

{"title":"Using systems biology approaches to study a multidrug resistance network","authors":"P. Dias, Catarina Costa, I. Sá-Correia, Miguel C. Teixeira, Pedro T. Monteiro, Arlindo L. Oliveira, A. T. Freitas","doi":"10.1109/ENBENG.2011.6026073","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026073","url":null,"abstract":"Multidrug resistance (MDR), a phenomenon with impact in Human Health and in Agro-Food and Environmental Biotechnology, often results from the activation of drug efflux pumps, many times controlled at the transcriptional level. The complex transcriptional control of these genes has been on the focus of our research, guided by the information gathered in the YEASTRACT database. In this paper, the approach used to elucidate the transcriptional control of FLR1, encoding a Saccharomyces cerevisiae Drug:H+ Antiporter, in response to stress induced by the fungicide mancozeb is explained. The transcription regulatory network underlying FLR1 activation was defined based on experimental data. Subsequently, a mathematical model describing this network was built and its response to mancozeb stress in different genetic backgrounds was simulated, using the Genetic Network Analyzer (GNA) software. This approach allowed the identification of essential features of the transition from unstressed to fungicide stressed cells and to make new predictions on the dynamical behavior of the system, which were validated experimentally. This work provides a good example of the successful combination of experimental and computational approaches in a systems biology perspective.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"279 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123143988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of targets and mechanisms of resistance to imatinib and quinine using a molecular systems biology approach 利用分子系统生物学方法鉴定对伊马替尼和奎宁的耐药靶点和机制

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026077

S. D. dos Santos, M. Palma, S. Tenreiro, N. Mira, A. Moreira, I. Sá-Correia

{"title":"Identification of targets and mechanisms of resistance to imatinib and quinine using a molecular systems biology approach","authors":"S. D. dos Santos, M. Palma, S. Tenreiro, N. Mira, A. Moreira, I. Sá-Correia","doi":"10.1109/ENBENG.2011.6026077","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026077","url":null,"abstract":"Saccharomyces cerevisiae provides an excellent eukary-otic model for biomedical and medicinal research. In this article, yeast was applied in pharmacological studies to identify new targets and mechanisms of action of drugs, as well as pathways involved in drug resistance. The work here presented is focused on two distinct drugs: the traditional anti-malarial quinine, and the paradigmatic anti-cancer imatinib. Several genome-wide approaches were employed, ranging from transcriptomics to chemogenomics and quantitative proteomics. The main results that stem from this work include the identification of potential new targets and modes of action for the two drugs studied. In particular, the highly conserved yeast vacuolar H+-ATPase was shown to be inhibited by imatinib, both in vivo and in vitro, suggesting that vacuolar function is a novel imatinib target, while quinine was found to inhibit the uptake of glucose into yeast cells following a competitive inhibition kinetic model. These findings might have an important parallel in the malaria parasite biology, where glucose uptake is vital and mediated by PfHT1, a single-copy transporter highly homologous to yeasts.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124689494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing methodologies to evaluate benefits and costs of Arterial Spin Labeling in Alzheimer's Disease 发展评估阿尔茨海默病动脉自旋标记的收益和成本的方法

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026091

M. Rodrigues, Monica D. Oliveira, M. T. Pena

引用次数: 0

Nano-bioabsorbent composite wound dressing for exudate management 用于渗出液处理的纳米生物吸收复合伤口敷料

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026058

Janete Alves, N. Belino, M. J. Geraldes

引用次数: 2

Assessment of Obstructive Sleep Apnea Syndrome by spectral analysis of physiological parameters 生理参数谱分析评价阻塞性睡眠呼吸暂停综合征

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026088

A. Coito, J. Sanches

{"title":"Assessment of Obstructive Sleep Apnea Syndrome by spectral analysis of physiological parameters","authors":"A. Coito, J. Sanches","doi":"10.1109/ENBENG.2011.6026088","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026088","url":null,"abstract":"Obstructive Sleep Apnea Syndrome (OSAS) is a very common sleep disorder that is associated with cardiovascular and neurocognitive impairments. The present study aims to assess the electroencephalographic (EEG) power before, during and after obstructive apnea episodes, in four frequency bands: delta (δ), theta (θ), alpha α and beta (β). Furthermore, Autonomic Nervous System (ANS) activity was assessed through the spectral analysis of heart rate variability (HRV) components in the three mentioned periods. For that purpose, continuous wavelet transform was applied to the EEG and RR (obtained from the ECG signal) signals. A new approach was carried out for assessing EEG power changes during an OSA episode: topographic EEG brain mapping. This technique was found to be a powerful tool to better visualize spectral changes during OSA episodes across the whole brain. The results demonstrate that there is a significant decrease in the EEG δ power during OSA, that is not totally recovered immediately after the episode. Since δ waves are linked to learning and synaptic plasticity processes, it is hypothesized that decreased δ power during OSA may contribute to the cognitive deterioration in patients with OSAS. The HRV analysis results show the presence of a pattern during OSA episodes, which is related to an impaired autonomic control of the heart in these patients.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"18 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128263653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Synthesis of monodispersed ORMOSIL nanoparticles and conjugation with DNA for gene therapy 单分散ORMOSIL纳米颗粒的合成及其与DNA的结合用于基因治疗

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026053

R. Colaço, M. C. Gonçalves, L. Gonçalves, A. Almeida, M. Martins

{"title":"Synthesis of monodispersed ORMOSIL nanoparticles and conjugation with DNA for gene therapy","authors":"R. Colaço, M. C. Gonçalves, L. Gonçalves, A. Almeida, M. Martins","doi":"10.1109/ENBENG.2011.6026053","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026053","url":null,"abstract":"Nanomedicine is an emerging new field combining nanotechnology and medicine. Silica nanoparticles are chemical and biologically inert, optically transparent and can be doped with imaging agents and/or functionalized to promote its conjugation with different therapeutic molecules. Silica nanoparticles can be engineered to improve diagnosis, treatment and follow-up of diseases. A combination of diagnosis devices and therapeutics (theranostics) would be beneficial for patients. In this work, ORMOSIL nanoparticles as non-viral vectors for gene delivery were prepared via a modified Stober sol-gel process directly with 3-aminopropyltriethoxysilane and tetraethylorthosilicate as precursors. Dynamic light scattering, transmission electron microscopy and Fourier transformed infrared spectroscopy were used to characterizing the hybrid nanospheres. Synthesis has been optimized and monodisperse spherical nanoparticles with desired size have been obtained. Nanoparticle-DNA complexes were successfully obtained at different ratios (nanoparticle/pDNA) and confirmed by agarose gel electrophoresis and ethidium bromide exclusion test.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131909498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of a two-electrode ECG acquisition system with dynamic interference rejection 动态抗干扰双电极心电采集系统的研制

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026084

C. M. Pereira, P. Mendes

引用次数: 8

Design and operation of bioreactor systems for the expansion of pluripotent stem cell-derived neural stem cells 多能干细胞衍生神经干细胞的生物反应器系统的设计和运行

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI: 10.1109/ENBENG.2011.6026041

C. Rodrigues, M. M. Diogo, C. L. da Silva, J. Cabral

{"title":"Design and operation of bioreactor systems for the expansion of pluripotent stem cell-derived neural stem cells","authors":"C. Rodrigues, M. M. Diogo, C. L. da Silva, J. Cabral","doi":"10.1109/ENBENG.2011.6026041","DOIUrl":"https://doi.org/10.1109/ENBENG.2011.6026041","url":null,"abstract":"Neurodegenerative disorders, such as Parkinson's disease, Huntington's disease, and multiple sclerosis, affect millions of people worldwide, with devastating impact on the patients and families. A reliable method to obtain cells for replacement therapy, using ex-vivo culture techniques, would be of great value for the treatment of these conditions. This project focuses on the development of bioreactor culture systems for the large-scale expansion of neural stem cells, starting with a model line of mouse embryonic stem cell-derived neural stem cells under adherent culture conditions. Adherent conditions are an alternative to conventional culture of NS cells as aggregates and may circumvent problems associated with this system. Small scale stirred bioreactors were successfully used for mNS cell expansion, with microcarriers to support cell adhesion and proliferation, with retention of neural stem/progenitor cell markers. The system was optimized by determining the best values for parameters like stirring speed, microcarrier concentration or feeding regimen. The system here described may open a new door for applications requiring high numbers of mouse neural cells, providing an efficient way for their generation. Furthermore, the know-how obtained with this work may be applied for the development of an equivalent system for human cells, which may find clinical applications.","PeriodicalId":206538,"journal":{"name":"1st Portuguese Biomedical Engineering Meeting","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121475696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2