Proceedings of Anticancer Research最新文献_第2页

Optimization of Polysaccharides Extraction from Physalis alkekengi L. Peel and Its Effect on the Expression of Inflammation-Related Proteins in SW620 Cells 多糖提取的优化及其对 SW620 细胞中炎症相关蛋白表达的影响

Proceedings of Anticancer Research Pub Date : 2024-08-09 DOI: 10.26689/par.v8i4.7906

Dan Shao, Chengxin Hao, Yubi Mei, Jiaxin Xu, Tianjiao Wang, Wei Yang, Jiamei Wu

{"title":"Optimization of Polysaccharides Extraction from Physalis alkekengi L. Peel and Its Effect on the Expression of Inflammation-Related Proteins in SW620 Cells","authors":"Dan Shao, Chengxin Hao, Yubi Mei, Jiaxin Xu, Tianjiao Wang, Wei Yang, Jiamei Wu","doi":"10.26689/par.v8i4.7906","DOIUrl":"https://doi.org/10.26689/par.v8i4.7906","url":null,"abstract":"Objective: To establish an optimized aqueous extraction process for polysaccharides from Physalis alkekengi L. peel and to preliminarily explore its in vitro anti-inflammatory activity against colorectal cancer SW620 cells. Methods: A single-factor test combined with orthogonal test analysis was used to evaluate the effects of the material-to-liquid ratio, extraction temperature, and extraction time on the yield of polysaccharides from Physalis alkekengi L. peel. The antioxidant activity of the polysaccharides was assessed by analyzing their free radical scavenging ability in vitro, and the anti-inflammatory effect was evaluated using SW620 cells. Results: The optimal extraction conditions were a material-to-liquid ratio of m(g):V(mL) = 1:30, an extraction temperature of 100°C, and an extraction time of 40 minutes, with a predicted polysaccharide yield of 25.7%. The polysaccharides from Physalis peruviana peel effectively scavenged DPPH, superoxide anion, and hydroxyl radicals. After treatment with Physalis peruviana polysaccharides, the levels of IL-1β, IL-18, and TNF-α in the cell culture medium were significantly reduced, and the phosphorylation level of P65 protein in SW620 cells was decreased. Conclusion: This extraction method is stable and reliable, and the prepared Physalis alkekengi L. polysaccharides exhibit significant in vitro antioxidant and anti-inflammatory activities. This study provides a theoretical basis for developing drugs for the prevention and treatment of colorectal cancer.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141922681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Effect of Tislelizumab Combined with Chemotherapy in the Treatment of Stage IIIb–IV Non-Small Cell Lung Cancer Tislelizumab 联合化疗治疗 IIIb-IV 期非小细胞肺癌的临床效果

Proceedings of Anticancer Research Pub Date : 2024-08-09 DOI: 10.26689/par.v8i4.7901

Jie Jiang

{"title":"Clinical Effect of Tislelizumab Combined with Chemotherapy in the Treatment of Stage IIIb–IV Non-Small Cell Lung Cancer","authors":"Jie Jiang","doi":"10.26689/par.v8i4.7901","DOIUrl":"https://doi.org/10.26689/par.v8i4.7901","url":null,"abstract":"Objective: To analyze the therapeutic effect of tislelizumab combined with chemotherapy in patients with stage IIIb–IV non-small cell lung cancer (NSCLC). Methods: A total of 50 patients with stage IIIb–IV NSCLC admitted between January 2022 and January 2024 were randomly divided into two groups using a random number table. The observation group included 25 cases treated with tislelizumab combined with chemotherapy, while the reference group included 25 cases treated with conventional chemotherapy. The clinical control rate, adverse reaction rate, tumor markers, immune function indicators, and quality of life scores were compared between the two groups. Results: The observation group had a higher clinical control rate and a lower adverse reaction rate compared to the reference group (P < 0.05). Before treatment, there were no significant differences in tumor markers, immune function indicators, and quality of life scores between the two groups (P > 0.05). Three months after treatment, the tumor marker levels in the observation group were lower than those in the reference group. Except for CD8+, all immune function indicators in the observation group were higher than those in the reference group, and the quality-of-life scores in the observation group were higher than those in the reference group (P < 0.05). Conclusion: Implementing tislelizumab combined with chemotherapy in patients with stage IIIb–IV NSCLC can improve the clinical control rate, reduce the adverse reaction rate, lower tumor marker levels, protect immune function, and improve quality of life.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141922266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of ZNF207 in Liver Hepatocellular Carcinoma: Expression Analysis and Prognostic Implications ZNF207 在肝脏肝细胞癌中的作用：表达分析和预后意义

Proceedings of Anticancer Research Pub Date : 2024-08-09 DOI: 10.26689/par.v8i4.7894

Chengrui Peng, Linfei Wu, Xiaofang Yang, Hanyun Yao, Yan Xie

{"title":"The Role of ZNF207 in Liver Hepatocellular Carcinoma: Expression Analysis and Prognostic Implications","authors":"Chengrui Peng, Linfei Wu, Xiaofang Yang, Hanyun Yao, Yan Xie","doi":"10.26689/par.v8i4.7894","DOIUrl":"https://doi.org/10.26689/par.v8i4.7894","url":null,"abstract":"Objective: To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma (LIHC) based on The Cancer Genome Atlas (TCGA) database. Methods: The mRNA sequencing data of 371 cases of primary liver cancer, 50 cases of normal tissues, and 3 cases of recurrent liver cancer were downloaded from the TCGA database. The corresponding clinical information of the 371 cases of hepatocellular carcinoma was subsequently analyzed. The difference in ZNF207 expression between normal and tumor tissues was analyzed using the UALCAN online database. The impact of ZNF207 expression on survival prognosis was assessed using the Kaplan-Meier method in R software. The GO and KEGG pathways of ZNF207 were analyzed. The Cox proportional hazards model was used to evaluate the prognostic factors of patients with LIHC. RT-qPCR was employed to verify the expression of ZNF207 in LIHC cells. Results: ZNF207 was highly expressed in LIHC tissues and HepG2 cells, with a significant difference (P < 0.05). Multivariate Cox regression analysis revealed that patients with high ZNF207 expression had a significantly shorter overall survival time compared to those with low ZNF207 expression (HR = 1.466, 95% CI: 1.011–2.126, P < 0.05). GO enrichment analysis suggested that ZNF207 may influence the onset and progression of hepatocellular carcinoma by regulating mRNA splicing and mRNA transcription processing through the spliceosome. KEGG pathway enrichment analysis indicated that ZNF207 might affect the onset and progression of hepatocellular carcinoma through mitophagy, mRNA surveillance, homologous recombination, spliceosome, and nuclear-cytoplasmic transport. Conclusion: The expression of ZNF207 may be an independent predictor of the prognosis of patients with LIHC and could influence the development of hepatocellular carcinoma through various gene functions and pathways. It has the potential to serve as a novel molecular marker for predicting the prognosis of hepatocellular carcinoma.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141923619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted Therapy of CEA-CAR-NK Cells Against Colorectal Cancer Cells 针对结直肠癌细胞的 CEA-CAR-NK 细胞靶向疗法

Proceedings of Anticancer Research Pub Date : 2024-07-17 DOI: 10.26689/par.v8i4.7607

Xinyu Zheng, Xiaomeng Chen, Xingzhou Xia, Wenzhen Wang, Qian Liu

{"title":"Targeted Therapy of CEA-CAR-NK Cells Against Colorectal Cancer Cells","authors":"Xinyu Zheng, Xiaomeng Chen, Xingzhou Xia, Wenzhen Wang, Qian Liu","doi":"10.26689/par.v8i4.7607","DOIUrl":"https://doi.org/10.26689/par.v8i4.7607","url":null,"abstract":"Objective: Investigate the cytotoxic effect of CAR-NK cells targeting CEA on colorectal cancer cells with positive CEA expression. Methods: The mRNA and protein levels of CEA in different CRC cell lines were detected by qRT-PCR and Western blot analysis. Lentiviral transduction was used to construct CAR-NK cells and empty vector CON-NK cells targeting CEA. Fluorescence microscopy and WB were used to determine whether the cells successfully constructed and expressed CAR structures. The effector NK cells were co-cultured with target cells, and the levels of LDH, IFN-γ, and GM-CSF were detected. The killing rate of effector cells was calculated, and the release of cytokines during the killing of target cells by different effector cells was compared. Results: The expression level of CEA in colorectal cancer patients was significantly higher than that in normal samples and other tumor samples, and the prognosis survival time of patients with high CEA expression was lower than that of CRC patients with low or no CEA expression (P < 0.05). The CEA expression of the HT29 cell line was significantly higher than that of the SW1116 cell line at both the mRNA and protein levels. CEA-CAR-NK92 cells and CON-NK92 cells expressed green fluorescence under a microscope, and WB results showed that CEA-CAR-NK92 cells successfully expressed the CAR structure. Compared with CON-NK92 cells and NK92 cells, CEA-CAR-NK92 cells effectively killed HT29 cells (P < 0.05). CEA-CAR-NK92 cells secreted a large amount of IFN-γ and GM-CSF during the killing of HT29 cells, while the cytokine secretion of CON-NK92 cells and NK92 cells was not significant (P < 0.05). Conclusion: CAR-NK92 cells targeting CEA can effectively kill CEA-positive colorectal cancer cells.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141829182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Value of MRI and CT in the Diagnosis of Retroperitoneal Tumours 核磁共振成像和 CT 在诊断腹膜后肿瘤中的价值

Proceedings of Anticancer Research Pub Date : 2024-07-11 DOI: 10.26689/par.v8i4.7518

Xinlian Jin, Ying Zhou

引用次数: 0

Analysis of the Clinical Value of Immunohistochemical Testing in the Pathological Diagnosis of Breast Cancer 免疫组化检验在乳腺癌病理诊断中的临床价值分析

Proceedings of Anticancer Research Pub Date : 2024-07-10 DOI: 10.26689/par.v8i4.7494

Qiushuang Ma, Jirui Sun, Haizhi Qiao, Xueli Wang, Jinku Zhang

{"title":"Analysis of the Clinical Value of Immunohistochemical Testing in the Pathological Diagnosis of Breast Cancer","authors":"Qiushuang Ma, Jirui Sun, Haizhi Qiao, Xueli Wang, Jinku Zhang","doi":"10.26689/par.v8i4.7494","DOIUrl":"https://doi.org/10.26689/par.v8i4.7494","url":null,"abstract":"Objective: To investigate the clinical value of immunohistochemistry (IHC) detection in the pathological diagnosis of breast cancer. Methods: Eighty breast cancer patients admitted to Baoding No. 1 Central Hospital from June 2022 to June 2023 were selected as study subjects. The samples were divided into a positive group (40 cases) and a negative group (40 cases) according to ER and PR test results. Immunohistochemistry was performed on all patients to compare the differences between the two groups in C-erbB-2 positive expression and axillary lymph node metastasis. Results: The positive expression rate of C-erbB-2 in the positive group (35.00%) was significantly lower than that in the negative group (80.00%), with a highly significant difference (P < 0.001). The axillary lymph node metastasis rate in the positive group (40.00%) was significantly lower than that in the negative group (75.00%), with a significant difference (P < 0.05). Conclusion: Immunohistochemical detection in breast cancer pathology enhances diagnostic accuracy, predicts prognosis, and supports personalized treatment by identifying ER, PR, and C-erbB-2. It is worth being widely adopted in clinical practice.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141662535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the Progress of Treating Gastroesophageal Reflux Disease with Modified Classic Traditional Chinese Medicine Prescription 改良经典中药方剂治疗胃食管反流病的进展研究

Proceedings of Anticancer Research Pub Date : 2024-06-04 DOI: 10.26689/par.v8i3.7079

Haojie Fu, Yahui Huang, Xiaonan Shi

引用次数: 0

Role of TSH Inhibition Therapy in the Postoperative Management of Patients with Differentiated Thyroid Cancer TSH 抑制疗法在分化型甲状腺癌患者术后管理中的作用

Proceedings of Anticancer Research Pub Date : 2024-04-12 DOI: 10.26689/par.v8i2.6295

Xudong Gao, Bo Zhao, Ya Su, Shenglin He

引用次数: 0

Effect of Sequential Early Enteral Nutrition on Postoperative Rehabilitation and Complications in Gastric Cancer Patients 序贯早期肠内营养对胃癌患者术后康复和并发症的影响

Proceedings of Anticancer Research Pub Date : 2024-04-10 DOI: 10.26689/par.v8i2.6413

Dongchou Han, Qiya Feng, Yingmei Fu, Feijian Zhang, Dazhen Chen, Junmei Wu

{"title":"Effect of Sequential Early Enteral Nutrition on Postoperative Rehabilitation and Complications in Gastric Cancer Patients","authors":"Dongchou Han, Qiya Feng, Yingmei Fu, Feijian Zhang, Dazhen Chen, Junmei Wu","doi":"10.26689/par.v8i2.6413","DOIUrl":"https://doi.org/10.26689/par.v8i2.6413","url":null,"abstract":"Objective: To analyze the effect of sequential early enteral nutrition in patients with gastric cancer after surgery. Methods: A total of 139 gastric cancer patients, treated between October 2021 and October 2023, were randomly selected and divided into two groups: Group A (68 cases, receiving early enteral nutrition) and Group B (71 cases, receiving sequential early enteral nutrition), using computer randomization. The effects of the interventions on both groups were compared. Results: Seven days post-operation, the levels of nutritional indicators in Group B were significantly higher than those in Group A (P < 0.05). Group B showed significantly better levels of inflammatory factors and immune factors compared to Group A seven days post-operation (P < 0.05). The postoperative complication rate in Group B was 4.23%, significantly lower than that in Group A, which was 16.18% (χ2 = 5.477, P = 0.019). Conclusion: The utilization of sequential early enteral nutrition in gastric cancer patients after surgery demonstrated notable improvements in nutritional status and inflammation markers, along with enhanced immunity, effectively reducing postoperative complications.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laparoscopic Surgery and Modified Xiaoyan Lidan Decoction: An Effective Combination for Treating Cholecystitis with Cholelithiasis 腹腔镜手术与改良小青囊煎剂：治疗胆囊炎合并胆石症的有效联合疗法

Proceedings of Anticancer Research Pub Date : 2023-12-22 DOI: 10.26689/par.v7i6.5613

Sidian Xiao

{"title":"Laparoscopic Surgery and Modified Xiaoyan Lidan Decoction: An Effective Combination for Treating Cholecystitis with Cholelithiasis","authors":"Sidian Xiao","doi":"10.26689/par.v7i6.5613","DOIUrl":"https://doi.org/10.26689/par.v7i6.5613","url":null,"abstract":"Objective: To assess the clinical effects of combining laparoscopic surgery with Modified Xiaoyan Lidan Decoction in patients diagnosed with cholecystitis and cholelithiasis. Methods: Following the guidelines of the double-blind method, 86 cases of cholecystitis with cholelithiasis were randomly divided into two groups, each comprising 43 cases. Both groups underwent laparoscopic surgery, with the observation group additionally receiving Modified Xiaoyan Lidan Decoction. A comparative analysis was conducted on clinical treatment effectiveness, general observation indicators, Traditional Chinese Medicine (TCM) syndrome scores, and the occurrence of adverse reactions between the two groups. Results: The observation group demonstrated a significantly higher overall clinical treatment effectiveness compared to the control group (P < 0.05). The clinical symptom improvement time and hospitalization time were shorter in the observation group, and the pain score and TCM syndrome score after treatment were lower than those in the control group (P < 0.05). No statistically significant difference was observed in the total reaction values (P > 0.05). Conclusion: The combined application of laparoscopic surgery and Modified Xiaoyan Lidan Decoction can enhance clinical treatment efficiency for patients with cholecystitis and cholelithiasis. It facilitates a quicker improvement in clinical symptoms without causing serious adverse reactions, suggesting its potential for widespread adoption.","PeriodicalId":20511,"journal":{"name":"Proceedings of Anticancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138944892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0