Probiotics and Antimicrobial Proteins最新文献_第6页

Fish-Derived Clostridium butyricum LZK-08: Genomic Insights and Probiotic Evaluation in Largemouth Bass. 鱼源丁酸梭菌LZK-08：大黑鲈基因组研究和益生菌评价。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-05 DOI: 10.1007/s12602-025-10721-0

Zhikun Liu, Jiaojiao Guo, Qingyang Su, Shikun Feng, Xindang Zhang, Yanmin Zhang, Guokun Yang, Xulu Chang, Xiaolin Meng

{"title":"Fish-Derived Clostridium butyricum LZK-08: Genomic Insights and Probiotic Evaluation in Largemouth Bass.","authors":"Zhikun Liu, Jiaojiao Guo, Qingyang Su, Shikun Feng, Xindang Zhang, Yanmin Zhang, Guokun Yang, Xulu Chang, Xiaolin Meng","doi":"10.1007/s12602-025-10721-0","DOIUrl":"https://doi.org/10.1007/s12602-025-10721-0","url":null,"abstract":"Clostridium butyricum has gained attention as a probiotic in aquaculture due to its ability to improve growth, gut health, and immune function. However, most strains currently used are derived from non-aquatic sources, which may limit their colonization and efficacy in fish. In this study, a novel strain, C. butyricum LZK-08, was isolated from the intestine of grass carp. The strain was identified based on morphological features, 16S rRNA sequencing, and whole-genome analysis. Its probiotic properties were assessed through comprehensive in vitro assays and in vivo trials in largemouth bass. LZK-08 exhibited strong acid (pH 4.0), bile salt (0.3%), and heat (up to 80 °C) tolerance, along with high auto-aggregation (92.3%) and notable surface hydrophobicity. It produced digestive enzymes (amylase, protease, lipase) and short-chain fatty acids, particularly butyrate (523.32 μg/mL). Genomic analysis revealed a 4.71 Mb genome composed of a circular chromosome and plasmid, encoding 4,207 protein-coding genes. Key genes were enriched in carbohydrate and amino acid metabolism, stress response, and adhesion. Feeding trials in largemouth bass showed that dietary supplementation with LZK-08 significantly improved growth parameters, reduced feed conversion ratio, enhanced intestinal antioxidant enzyme and digestive enzyme activities. Histological analysis revealed improved intestinal morphology, including increased villus height and muscle thickness. 16S rRNA sequencing further showed that LZK-08 modulated gut microbiota by enriching beneficial taxa such as Lactobacillus and reducing potentially unfavorable Romboutsia. These findings indicate that C. butyricum LZK-08 is a physiologically robust, and genomically safe probiotic with strong functional attributes, demonstrating high potential for use in sustainable aquaculture.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Shredded Potato and Research on Their Prebiotic, Antioxidant, and Fermentative Properties. 传统发酵马铃薯丝中乳酸菌的分离鉴定及其益生元、抗氧化和发酵特性研究

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-03 DOI: 10.1007/s12602-025-10746-5

Nan Hu, Jie Ji, Hongji Zhao, Jingyi Sun, Shanzi Cong

{"title":"Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Shredded Potato and Research on Their Prebiotic, Antioxidant, and Fermentative Properties.","authors":"Nan Hu, Jie Ji, Hongji Zhao, Jingyi Sun, Shanzi Cong","doi":"10.1007/s12602-025-10746-5","DOIUrl":"10.1007/s12602-025-10746-5","url":null,"abstract":"The aim of this study was to screen lactic acid bacteria (LAB) suitable for starch-based fermentation from traditional fermented shredded potatoes (TFSP). Analysis of the microbial diversity by 16S rDNA sequencing in TFSP revealed that LAB were a genus with high relative abundance of the bacteria population. Thirty LAB strains were isolated and purified from TFSP. Ten LAB strains with sequence similarity greater than 98% from TFSP were obtained, and the fermentation, antioxidant, and probiotic properties were tested. The results showed that 10 strains had acid-producing abilities. Lactiplantibacillus plantarum ZYN-03, ZYN-04, ZYN-05, ZYN-09, and ZYN-10 showed strong extracellular enzyme activity and SOD enzyme activity. Lacticaseibacillus rhamnosus ZYN-08 showed a higher survival rate in 8% ethanol (v/v). Salt tolerance and nitrite degradation capacity assays revealed that Lp. plantarum ZYN-05 and ZYN-09 had high survival rates. Lp. plantarum ZYN-05 and ZYN-10 and L. rhamnosus ZYN-08 displayed good antioxidant activity, while Lp. plantarum ZYN-01 and ZYN-04 and L. rhamnosus ZYN-08 had viable counts of LAB above 5.5 log CFU/mL in gastric acid, bile, and in vitro simulated cultures. Therefore, LAB isolated from TFSP have good fermentation capacity, probiotic properties, and antioxidant activities, and have the potential application for the fermentation of plant-based raw materials.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus salivarius CCFM1416 Modulates Oral Immunity via IL-17/IKBζ and Combats Candida albicans Infection. 唾液链球菌CCFM1416通过IL-17/IKBζ调节口腔免疫并对抗白色念珠菌感染

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-03 DOI: 10.1007/s12602-025-10723-y

Lijiang Ding, Yanxiao Zhang, Qiuxiang Zhang, Xin Tang, Yueyan Wang, Bingyong Mao, Jianxin Zhao, Shumao Cui

{"title":"Streptococcus salivarius CCFM1416 Modulates Oral Immunity via IL-17/IKBζ and Combats Candida albicans Infection.","authors":"Lijiang Ding, Yanxiao Zhang, Qiuxiang Zhang, Xin Tang, Yueyan Wang, Bingyong Mao, Jianxin Zhao, Shumao Cui","doi":"10.1007/s12602-025-10723-y","DOIUrl":"10.1007/s12602-025-10723-y","url":null,"abstract":"Oropharyngeal candidiasis (OPC) commonly occurs in immunocompromised individuals, and enhancing endogenous antimicrobial defenses with probiotics has emerged as an effective prevention strategy. Given the critical role of human β-defensin-2 (HBD-2) in combating Candida albicans infection in the oral cavity, this study aimed to identify oral commensal bacteria promoting HBD-2 production in the host. Thirty-five Streptococcus salivarius strains were screened using the HOK-16B cell line, and two were identified as significantly promoting HBD-2 secretion. These strains were further validated in a murine OPC model. The results indicated that oral intervention with S. salivarius CCFM1416 activated the IL-17/IKBζ signaling pathway in the host (IKBζ from 67.73 to 122.27 pg/mg), upregulating the expression of β-defensin (from 13.64 to 25.21 pg/mg). Consequently, C. albicans colonization and adhesion in the oral cavity were markedly reduced. Finally, a 14-day oral toxicity study demonstrated that oral administration of S. salivarius CCFM1416 is safe in vivo. In summary, the research results indicated that S. salivarius CCFM1416 can effectively prevent the occurrence of OPC and is an excellent probiotic candidate for the prevention of oral mucosal infections.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and Phenotypic Evaluation of the Gliadin-Degrading Probiotic Bacillus amyloliquefaciens EG025 from Cheonggukjang for Celiac Disease Treatment. 清果酱解淀粉芽孢杆菌EG025治疗乳糜泻的基因组和表型评价

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-02 DOI: 10.1007/s12602-025-10728-7

Jinchul Jo, Seoae Cho, Heebal Kim

{"title":"Genomic and Phenotypic Evaluation of the Gliadin-Degrading Probiotic Bacillus amyloliquefaciens EG025 from Cheonggukjang for Celiac Disease Treatment.","authors":"Jinchul Jo, Seoae Cho, Heebal Kim","doi":"10.1007/s12602-025-10728-7","DOIUrl":"https://doi.org/10.1007/s12602-025-10728-7","url":null,"abstract":"Bacillus amyloliquefaciens EG025 was isolated from cheonggukjang, a traditional Korean fermented soybean paste. Among multiple isolates from diverse fermented foods, B. amyloliquefaciens EG025 uniquely exhibited potent gliadin-degrading activity, a trait with the potential to attenuate the immunogenicity of gluten peptides implicated in celiac disease. B. amyloliquefaciens EG025 maintained robust enzymatic activity across a wide pH spectrum (neutral to alkaline) and, remarkably, retained over 90.4% of its maximal activity following a 2-h exposure to pH 2. In addition to its gliadin-degrading capability, B. amyloliquefaciens EG025 demonstrated exceptional acid and bile tolerance, indicating a strong capacity to survive and function under the harsh conditions of the human gastrointestinal tract. Whole-genome sequencing and comprehensive annotation revealed biosynthetic gene clusters for secondary metabolites known to confer host benefits, alongside key probiotic functional genes involved in gliadin degradation, acid and bile resistance, stress response, mucosal adhesion, and de novo vitamin biosynthesis. Importantly, safety assessment confirmed the absence of concerning antibiotic resistance determinants or virulence factors, underscoring the strain's safety profile. Phylogenetic reconstruction further highlighted the genetic distinctiveness of EG025 relative to other B. amyloliquefaciens strains, emphasizing its novelty within the species. Collectively, these combined in vitro assay, genomic, and phylogenetic results underscore the significant probiotic potential of B. amyloliquefaciens EG025 and support its future development as a novel therapeutic candidate for improving gluten tolerance and managing celiac disease. Its safety and multifunctional attributes position it as a promising ingredient for functional foods targeted at gluten-sensitive populations.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety of One of the Most Commonly Used Probiotic Strains: Systematic Review and Meta-analysis of Reported Adverse Events. 一种最常用的益生菌菌株的安全性：报告不良事件的系统回顾和荟萃分析。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-02 DOI: 10.1007/s12602-025-10740-x

Aneesha Kumar, Daniel Merenstein, Tina Tan, Peggy-Ita Obeng-Nyarkoh, Iva Hojsak, Frank D'Amico

{"title":"Safety of One of the Most Commonly Used Probiotic Strains: Systematic Review and Meta-analysis of Reported Adverse Events.","authors":"Aneesha Kumar, Daniel Merenstein, Tina Tan, Peggy-Ita Obeng-Nyarkoh, Iva Hojsak, Frank D'Amico","doi":"10.1007/s12602-025-10740-x","DOIUrl":"https://doi.org/10.1007/s12602-025-10740-x","url":null,"abstract":"Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. Probiotics are widely used in everyday foods and supplements but very few studies have thoroughly evaluated safety. We evaluated adverse events (AEs) for one of the most widely used strains, Bifidobacterium animalis subsp. lactis (BB-12). A systematic review (SR) and meta-analysis (MA) were conducted to evaluate adverse events in human trials using BB-12. Two independent reviewers separately searched for randomized controlled trials (RCTs) using the following mesh words for inclusion: English language, double-blinded, randomized or random allocation, and placebo-controlled trial of BB-12. One hundred and eighty-one studies were identified of which 35 qualified for inclusion. All authors of the 35 studies were contacted to perform an individual patient data meta-analysis (IPDMA). Results from the MA studies showed there was no statistical difference in the rates of AE between the probiotic (15.2%) and placebo (14%) groups, odds ratio = 1.10, 95% confidence interval (0.88, 1.36), p = .40. Further, results from the IPDMA showed statistically similar unadjusted (27% vs. 25%) and adjusted rates of AE (43% vs. 41%) between BB-12 and placebo, with the relative risk of AE being 1.05, 95% confidence interval (.94, 1.15). Both meta-analysis approaches showed no difference in the rates of AE for the BB-12 and placebo groups. The data support the safety of wide usage of BB-12. However, half of the RCTs analyzed for the MA did not report AE data. This is a major limitation of published literature. This study has been registered in PROSPERO: https://www.crd.york.ac.uk/PROSPERO/view/CRD42023434556.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Next-Generation Probiotics in Colorectal Cancer Pathways: Mechanisms and Therapeutic Potential. 下一代益生菌在结直肠癌通路中的作用：机制和治疗潜力。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-01 DOI: 10.1007/s12602-025-10745-6

Sheikh Saba Naz, Josep Sabate Ortega

{"title":"The Role of Next-Generation Probiotics in Colorectal Cancer Pathways: Mechanisms and Therapeutic Potential.","authors":"Sheikh Saba Naz, Josep Sabate Ortega","doi":"10.1007/s12602-025-10745-6","DOIUrl":"https://doi.org/10.1007/s12602-025-10745-6","url":null,"abstract":"Colorectal cancer (CRC) is a second leading cause of human mortality worldwide. Due to the inconsistent effectiveness of traditional anticancer therapies, there is an increasing need for novel drug-free cancer therapy that enhances the efficacy of current treatments with minimum side effects. CRC is linked to microbiome modifications, a phenomenon termed dysbiosis, which entails the reduction and/or augmentation of certain gut bacteria and their metabolites. Administration of next-generation probiotics (NGP) may serve as an effective and accessible cancer treatment by regulating gut microbiota composition. The review provides a focused analysis of NGPs in CRC, highlighting their preventive and therapeutic roles, modulation of gut microbiota and signaling pathways, and current translational progress. Restoration of intestinal microbiota in cancer patients may promote anti-inflammatory, anticarcinogenic, immunomodulatory, and other physiologically significant pathways that exhibit high selectivity for tumor cells. The potential advantages of NGP include the modulation of intestinal microbial community and functionality, in addition to influencing the host's inflammatory response. NGPs that help maintain gut balance include Bacteroides fragilis, Akkermansia muciniphila, Propionibacterium freudenreichii, and specific strains of Clostridium spp. This review emphasizes their applicability in CRC prevention, with promising preclinical evidence from animal models. Recent evidence suggests that NGP may provide novel therapeutic approaches for CRC by facilitating physiological processes via the generation of immuno-modulatory metabolites that counteract CRC. Despite the observed positive outcomes in preclinical studies, more research concentrating on targeted strain, safe use, and dosage specificity is necessary to confirm the effectiveness and safety profile of NGP in the treatment of CRC.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postbiotics and Parabiotics in Veterinary Medicine: A Market Overview. 兽药中的后生物制剂和副生物制剂：市场综述。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-01 DOI: 10.1007/s12602-025-10734-9

Seyede Hanieh Hosseini, Houshmand Sharafi, Amirhossein Emamjomeh, Fatemeh Nasri, Ali Hosseini, Sepideh Mardani, Mehran Moradi

{"title":"Postbiotics and Parabiotics in Veterinary Medicine: A Market Overview.","authors":"Seyede Hanieh Hosseini, Houshmand Sharafi, Amirhossein Emamjomeh, Fatemeh Nasri, Ali Hosseini, Sepideh Mardani, Mehran Moradi","doi":"10.1007/s12602-025-10734-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10734-9","url":null,"abstract":"Postbiotics and parabiotics (PP) are innovative concepts in veterinary medicine that have garnered substantial interest owing to their unique properties and potential benefits. PP offers a promising alternative to live bacteria, demonstrating properties such as prebiotic, immunomodulatory, antimicrobial, anti-inflammatory, antioxidant, and anticancer effects, while potentially mitigating some of the common challenges associated with live bacterial applications. PP products are manufactured and commercially employed in livestock, poultry, and pets. Moreover, postbiotic-based commercial products have exhibited a diverse range of beneficial effects including enhanced gut health and microbiota, strengthened immune modulation, stress mitigation, and improved animal production. Moreover, their antimicrobial and anti-inflammatory properties make them viable dietary supplements or additives, potentially replacing antibiotics and addressing the growing concern of antibiotic resistance in the livestock industry. This work reviews the commercial applications of PP in animal nutrition and therapeutics, including in poultry, cattle, sheep, horses, and pet animals. The growing demand for PP products shows that we need to keep researching to find new uses for PP.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Enterocin A in Saccharomyces cerevisiae. 肠蛋白A在酿酒酵母中的表达。

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-08-30 DOI: 10.1007/s12602-025-10686-0

Michelle Rossouw, Gerhardt Coetzee, Rosemary A Cripwell, Willem H van Zyl, Leon M T Dicks, Carla L Ritter, Marinda Viljoen-Bloom

{"title":"Expression of Enterocin A in Saccharomyces cerevisiae.","authors":"Michelle Rossouw, Gerhardt Coetzee, Rosemary A Cripwell, Willem H van Zyl, Leon M T Dicks, Carla L Ritter, Marinda Viljoen-Bloom","doi":"10.1007/s12602-025-10686-0","DOIUrl":"https://doi.org/10.1007/s12602-025-10686-0","url":null,"abstract":"Recombinant expression in the yeast Saccharomyces cerevisiae offers an alternative approach to developing large-scale production systems for class II bacteriocins from lactic acid bacteria, such as enterocin A, mundticin ST4SA and plantaricin 423. An important consideration for bacteriocin activity is disulphide bond formation: mature mundticin ST4SA has one, and plantaricin 423 and enterocin A each have two disulphide bonds. The native bacteriocin operon typically includes accessory proteins that facilitate disulphide bond formation, but this gene is absent in the enterocin A operon. In this study, the recombinant expression of a codon-optimised gene for enterocin A in S. cerevisiae, was compared to that for a codon-optimised plantaricin 423 and mundticin ST4SA, previously successfully expressed in S. cerevisiae. Shake flasks delivered more than twofold higher peptide EntA_Opt levels than PlaX_Opt and MunX_Opt, with even higher EntA_Opt expression levels in batch fermentations. However, the bacteriocin activity of plantaricin 423 was considerably lower than that of enterocin A and mundticin ST4SA. It is postulated that this could be a result of incorrect disulphide bond conformation due to the absence of the plantaricin accessory protein, PlaC, in S. cerevisiae. Nano-LC-MS/MS analysis showed various post-translational modifications for the peptides, with a greater proportion of EntA_Opt peptides with the correct disulphide bond conformation than for PlaX_Opt. This study demonstrated that S. cerevisiae is a promising host to produce recombinant class IIa bacteriocins, particularly enterocin A. However, the co-expression of accessory proteins should be investigated to improve the activity of recombinant plantaricin 423.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation into the Anti-hyperglycemic Traits and Bioactive Constituents of Postbiotics Derived from Lacticaseibacillus paracasei NCUH012072. 副干酪乳杆菌NCUH012072后生制剂抗高血糖特性及活性成分研究

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-08-29 DOI: 10.1007/s12602-025-10726-9

Yazhou Xu, Tao Xiong, Linli Zhang, Xinyi Kang, Fang Ma, Mingwei Zhao

{"title":"Investigation into the Anti-hyperglycemic Traits and Bioactive Constituents of Postbiotics Derived from Lacticaseibacillus paracasei NCUH012072.","authors":"Yazhou Xu, Tao Xiong, Linli Zhang, Xinyi Kang, Fang Ma, Mingwei Zhao","doi":"10.1007/s12602-025-10726-9","DOIUrl":"https://doi.org/10.1007/s12602-025-10726-9","url":null,"abstract":"Type 2 diabetes (T2D) and its associated complications have emerged as significant global public health challenges. Postbiotics have shown potential benefits in managing T2D. To identify effective postbiotics for T2D amelioration and elucidate the material basis underlying their efficacy, this study developed an anti-hyperglycemic evaluation model. Using an LC-MS platform combined with bioinformatics approaches, we comprehensively analyzed the metabolic distribution profiles of postbiotics in cell-free supernatant (CFS) and cell-free extract (CFE). Through this analysis, we identified a series of notable differential metabolites, including alpha amino acids, D-gulono-1,4-lactone, and glutathione. Spectrum-effect analysis was employed to preliminarily investigate the functional active substances responsible for the anti-hyperglycemic effects of postbiotics, revealing compounds potentially associated with anti-hyperglycemic properties, such as pyroglutamic acid and fumaric acid. Additionally, ultra-filtration mass spectrometry experiments were conducted to target and isolate potential bioactive compounds interacting with α-amylase, α-glucosidase, and tyrosine phosphatase 1B (PTP-1B), including mesoxalic acid, γ-glutamylphenylalanine, and L-3-phenyllactic acid.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Probiotic Properties and Cholesterol Metabolism Regulation Potential of Lactiplantibacillus plantarum QL-19. 植物乳杆菌QL-19益生菌特性及胆固醇代谢调控潜力的研究

IF 4.4 2区生物学

Probiotics and Antimicrobial Proteins Pub Date : 2025-08-29 DOI: 10.1007/s12602-025-10724-x

Mingkai Liu, Yanlei Han, Shanshan Wang, Yiming Wang, Hui Xu

{"title":"Characterization of the Probiotic Properties and Cholesterol Metabolism Regulation Potential of Lactiplantibacillus plantarum QL-19.","authors":"Mingkai Liu, Yanlei Han, Shanshan Wang, Yiming Wang, Hui Xu","doi":"10.1007/s12602-025-10724-x","DOIUrl":"https://doi.org/10.1007/s12602-025-10724-x","url":null,"abstract":"Lactic acid bacteria (LAB) play a crucial role in food fermentation and human health. This study introduces Lactiplantibacillus plantarum (L. plantarum) QL-19, a novel probiotic strain isolated from traditional Hunan pickles and characterized via whole-genome sequencing and in vitro functional assays. The 3,396,702 genome contains 3236 predicted coding sequences, including genes associated with stress tolerance, adhesion, and cholesterol metabolism (cbh, cfa, and hydroxymethylglutaryl-CoA synthase). Genomic analysis further revealed biosynthetic gene clusters encoding antimicrobial and antioxidant compounds, emphasizing its functional versatility. A comprehensive safety assessment confirmed that QL-19 is non-pathogenic and non-hemolytic, meeting probiotic safety standards. In HepG2 cell-based experiments, L. plantarum QL-19 reduced intracellular total cholesterol levels. RT-qPCR and Western blotting were employed to evaluate the expression of genes and proteins involved in cholesterol metabolism. The results demonstrated that QL-19 enhanced cholesterol metabolism by upregulating the expression of SREBP2, LDLR, and CYP7A1, while downregulating NPC1L1 and HMGCR. The results suggest that QL-19 facilitates cholesterol metabolism through multiple pathways. Taken together with its 75.4% in vitro cholesterol degradation rate, these findings highlight the potential of QL-19 as a cholesterol-lowering probiotic candidate for cardiovascular health applications.","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0