Preventive Nutrition and Food Science最新文献_第3页

Characterization of Rhodiola heterodonta (Crassulaceae): Phytocomposition, Antioxidant and Antihyperglycemic Activities. 红景天（十字花科）的特征：植物成分、抗氧化和降血糖活性。

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.135

Zaripova Mr, Gayibova Sn, Makhmudov Rr, Mamadrahimov Aa, Vypova Nl, Gayibov Ug, Miralimova Sm, Aripov Tf

引用次数: 0

Effect of Green Tea Supplementation on Inflammatory Markers among Patients with Metabolic Syndrome and Related Disorders: A Systematic Review and Meta-Analysis. 补充绿茶对代谢综合征及相关疾病患者炎症标志物的影响：系统回顾与元分析》。

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.106

Fernanda Souza de Oliveira Assis, Gabriel Lima Vasconcellos, Diego José Pereira Lopes, Leandro Roberto de Macedo, Maísa Silva

{"title":"Effect of Green Tea Supplementation on Inflammatory Markers among Patients with Metabolic Syndrome and Related Disorders: A Systematic Review and Meta-Analysis.","authors":"Fernanda Souza de Oliveira Assis, Gabriel Lima Vasconcellos, Diego José Pereira Lopes, Leandro Roberto de Macedo, Maísa Silva","doi":"10.3746/pnf.2024.29.2.106","DOIUrl":"10.3746/pnf.2024.29.2.106","url":null,"abstract":"Several randomized controlled trials (RCTs) have investigated the potential benefits of green tea on the inflammatory process in metabolic syndrome (MetS). However, the results are inconclusive and inconsistent. In the present study, we performed a literature review and meta-analysis to evaluate the effect of green tea supplementation on inflammatory markers [e.g., tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), and interleukin-6 (IL-6)] among patients with MetS and related disorders. We systematically searched for relevant publications up to March 2022 in the PubMed, Scopus, Web of Science, and SciELO databases. The review was registered with PROSPERO (CRD42022320345). Mean differences with 95% confidence intervals were pooled on the basis of the random effects model to compare the effects of green tea with placebo. We used meta-regression and subgroup analyses to determine the cause of heterogeneity and performed study quality assessment using the Grading of Recommendations Assessment, Development, and Evaluation method. We assessed publication bias using funnel plots and Egger's tests. Out of the total 15 RCTs that were included in this systematic review, 12 were chosen for the meta-analysis. The results revealed that green tea significantly decreased TNF-α levels but did not affect CRP and IL-6 levels. Subgroup analysis showed that green tea supplementation in studies lasting ≤8 weeks significantly increased CRP levels. Furthermore, meta-regression analysis demonstrated a significant association between increasing IL-6 concentration and treatment duration. According to our meta-analysis, green tea was shown to considerably lower circulating TNF-α levels. To confirm these findings, carefully planned trials are required.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"106-117"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Enzyme-Assisted Extraction from Ginger (Zingiber officinale) Leaf and Its Immune-Stimulating Effects on Macrophages. 优化生姜叶的酶辅助提取及其对巨噬细胞的免疫刺激作用

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.228

Hanbi Kim, Mina Kim, Sangnam Oh, Yujin Hwang, Jeong-Sook Choe

{"title":"Optimization of Enzyme-Assisted Extraction from Ginger (Zingiber officinale) Leaf and Its Immune-Stimulating Effects on Macrophages.","authors":"Hanbi Kim, Mina Kim, Sangnam Oh, Yujin Hwang, Jeong-Sook Choe","doi":"10.3746/pnf.2024.29.2.228","DOIUrl":"10.3746/pnf.2024.29.2.228","url":null,"abstract":"The ginger leaves contain terpenoids and phenolic compounds, such as gingerol and shogaol, which exert various physiological effects. This study focused on determining the optimal conditions for an enzyme (Ultimase MFC) extraction to enhance the bioactive components of underutilized ginger leaves using the response surface method. The extracted material was evaluated in terms of its yield and antioxidant capacity (total phenolic content, total flavonoid content, and activities of 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid). As a result, the optimal conditions included an enzyme concentration of 0.1% (v/v), a liquid-solid ratio of 33.939 mL/g, and an extraction time of 4 h. The optimized conditions resulted in an improvement in yield and antioxidant capacity, except for the total phenolic content of ginger leaves, when compared to the reference control extract. Additionally, the possibility of improving immunity was confirmed as nitric oxide and cytokines increased in macrophage cells compared with non-treatment control. Therefore, these extraction conditions enhance the potential industrial value of ginger leaves and underscore their promise as a natural ingredient for functional foods.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"228-236"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of Nicotinamide and Agastache rugosa Extract: A Potent Strategy for Protecting Hs68 Cells from UVB-Induced Photoaging. 烟酰胺与姬松茸提取物的结合：保护 Hs68 细胞免受紫外线诱导的光老化的有效策略

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.162

Seungjoo Baik, Huijin Heo, Seonghwa Hong, Heon Sang Jeong, Junsoo Lee, Hana Lee

{"title":"Combination of Nicotinamide and Agastache rugosa Extract: A Potent Strategy for Protecting Hs68 Cells from UVB-Induced Photoaging.","authors":"Seungjoo Baik, Huijin Heo, Seonghwa Hong, Heon Sang Jeong, Junsoo Lee, Hana Lee","doi":"10.3746/pnf.2024.29.2.162","DOIUrl":"10.3746/pnf.2024.29.2.162","url":null,"abstract":"This study investigated the protective effects of nicotinamide (NAM) and Agastache rugosa extract (AR) against ultraviolet B (UVB)-induced photoaging in Hs68 cells. The results demonstrated that NAM and AR, alone or in combination, exhibited concentration-dependent protective effects against UVB radiation. The highest synergistic effect was observed at a NAM:AR ratio of 6:4. This combination exhibited a synergistic protective effect against UVB-induced photoaging. The sample concentration required for 80% cell survival was 9.70 μM and 131.16 ppm for NAM and AR, respectively. However, when combined, they exhibited strong synergistic effects with concentrations as low as 0.11 μM and 17.50 ppm. Moreover, 5.26 μM of NAM and 1,082.13 ppm of AR were required to inhibit 30% of reactive oxygen species, but the combination treatment required 0.62 μM and 95.49 ppm, respectively. This combination significantly reduced the production of matrix metalloproteinase and increased collagen production. These findings highlight the potential of combining NAM and AR as functional cosmetic materials to protect against UVB-induced photoaging. The synergistic effects observed in this study provide valuable information for developing novel strategies for cosmetic combinations that target UVB-mediated skin damage.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"162-169"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation Characteristics of Unripe Citrus unshiu Vinegar Production Using Acetic Acid Bacteria Isolated from Traditional Fermented Vinegars. 利用从传统发酵醋中分离出的醋酸菌生产未成熟柑橘未熟醋的发酵特性。

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.220

Sang-Hun Won, Yeong-Jun Kim, Kyu-Taek Choi, Jun-Su Choi, Heui-Dong Park, Sae-Byuk Lee

{"title":"Fermentation Characteristics of Unripe Citrus unshiu Vinegar Production Using Acetic Acid Bacteria Isolated from Traditional Fermented Vinegars.","authors":"Sang-Hun Won, Yeong-Jun Kim, Kyu-Taek Choi, Jun-Su Choi, Heui-Dong Park, Sae-Byuk Lee","doi":"10.3746/pnf.2024.29.2.220","DOIUrl":"10.3746/pnf.2024.29.2.220","url":null,"abstract":"Here, we aimed to isolate an acetic acid bacterium that is suitable for the production of unripe Citrus unshiu vinegar from traditional fermented vinegars. We compared the halo sizes of isolates to select a strain with superior acetic acid production capabilities and selected Komagataeibacter kakiaceti P6 (P6) as the final strain. Using Acetobacter pasteurianus CY (CY) and A. pasteurianus KACC 17058 (KACC 17058) as controls, we analyzed the total phenolic compounds, total flavonoid content, antioxidant activities, and organic acids of the selected strain to verify its suitability for acetic acid fermentation. On the 30th day of the fermentation period, P6 showed a total acidity of 4.86%, which was higher than that of control groups (CY, 4.16%; KACC 17058, 4.01%). The total phenolic compounds, total flavonoid content, 1,1-diphenyl-2-picrylhydrazyl scavenging activity, and ferric ion reducing antioxidant power values significantly increased during fermentation with P6 compared with the initial C. unshiu wine, and no significant differences were observed from the vinegars produced by CY and KACC 17058. Moreover, organic acid analysis revealed that the unripe C. unshiu vinegar produced with P6 had an acetic acid content of 26.15 mg/mL, which was significantly higher than those produced with CY and KACC 17058, indicating that the P6 strain effectively produces acetic acid without adversely affecting other quality aspects during fermentation. In conclusion, the novel P6 strain is expected to be used as a starter for fermenting unripe C. unshiu vinegar, and its excellent acetic acid production capabilities suggest potential applications for other vinegars.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"220-227"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rose Petal Extract Ameliorates Obesity in High Fat Diet-Induced Obese Mice. 玫瑰花瓣提取物可改善高脂饮食诱发的肥胖小鼠的肥胖症。

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.125

Jaeeun Jung, Minhee Lee, Seong-Hoo Park, Wonhee Cho, Jinhak Kim, Sangwon Eun, Jeongmin Lee

{"title":"Rose Petal Extract Ameliorates Obesity in High Fat Diet-Induced Obese Mice.","authors":"Jaeeun Jung, Minhee Lee, Seong-Hoo Park, Wonhee Cho, Jinhak Kim, Sangwon Eun, Jeongmin Lee","doi":"10.3746/pnf.2024.29.2.125","DOIUrl":"10.3746/pnf.2024.29.2.125","url":null,"abstract":"In Asia, Rosa spp. has been used in traditional medicine for the treatment of osteoarthritis, rheumatoid arthritis, and edema. In this study, we investigated the effect of rose petal extract (RPE) on high fat diet (HFD)-induced obesity in mice. C57BL/6J mice were fed with either an AIN-93G diet (normal control), a 60% HFD, or a HFD plus supplementation with RPE at 100 or 200 mg/kg body weight (HFD+R100, HFD+R200) for 14 weeks. The HFD increased the body weight gain, liver and fat weight, lipid profiles (total cholesterol, triglyceride, high density lipoprotein cholesterol, and low density lipoprotein cholesterol), and the serum aspartate aminotransferase and alanine aminotransferase levels of mice, while RPE supplementation significantly decreased these parameters compared with the HFD group. Furthermore, the HFD increased the protein expressions of adipogenesis- and lipogenesis-related factors and decreased the protein expression of lipolysis- and energy metabolism-related factors. Conversely, RPE supplementation significantly decreased the protein expression of adipogenesis- and lipogenesis-related factors and increased the protein expression of lipolysis- and energy metabolism-related factors compared to the HFD group. Taken together, the results provide preliminary evidence for the potential protective effects of the RPE against obesity.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"125-134"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Research on the Role of Isomaltooligosaccharides in Gastrointestinal Health and Metabolic Diseases. 关于异麦芽寡糖在胃肠道健康和代谢性疾病中的作用的当前研究。

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.93

Dina Mustika Rini, Wenxi Xu, Takuya Suzuki

{"title":"Current Research on the Role of Isomaltooligosaccharides in Gastrointestinal Health and Metabolic Diseases.","authors":"Dina Mustika Rini, Wenxi Xu, Takuya Suzuki","doi":"10.3746/pnf.2024.29.2.93","DOIUrl":"10.3746/pnf.2024.29.2.93","url":null,"abstract":"The intestinal epithelium plays an important role in maintaining the intestinal barrier and facilitating nutrient absorption. It also serves as a critical physical barrier against the infiltration of foreign substances from the intestinal lumen into the circulation. Intestinal barrier dysfunction has been implicated in the development of several diseases. Isomaltooligosaccharides (IMOs), which are a type of dietary fiber, possess multiple health benefits. However, there is limited information regarding their efficacy against gastrointestinal diseases. This review explores the therapeutic potential of IMOs in obesity, diabetes mellitus, inflammatory bowel disease (IBD), hyperlipidemia, and constipation. High-fat diet (HFD)-induced obesity models have shown that IMOs, administered alone or in combination with other compounds, exhibit potent antiobesity effects, making them promising agents in the treatment of obesity and its associated complications. Moreover, IMOs exhibit preventive effects against HFD-induced metabolic dysfunction by modulating gut microbiota and short-chain fatty acid levels, thereby ameliorating symptoms. Furthermore, IMOs can reduce IBD and alleviate hyperlipidemia, as indicated by the reduced histological colitis scores and improved lipid profiles observed in clinical trials and animal studies. This review highlights IMOs as a versatile intervention strategy that can improve gastrointestinal health by modulating gut microbiota, immune responses, and metabolic parameters, providing a multifaceted approach to address the complex nature of gastrointestinal disorders.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"93-105"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Korean Mint (Agastache rugosa) Extract and Its Bioactive Compound Tilianin Alleviate Muscle Atrophy via the PI3K/Akt/FoxO3 Pathway in C2C12 Myotubes. 韩国薄荷（Agastache rugosa）提取物及其生物活性化合物 Tilianin 可通过 PI3K/Akt/FoxO3 通路缓解 C2C12 肌细胞的肌肉萎缩。

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.154

Yu Kyong Woo, Minseong Kang, Changhee Kim, Jae-Kwan Hwang

{"title":"Korean Mint (Agastache rugosa) Extract and Its Bioactive Compound Tilianin Alleviate Muscle Atrophy via the PI3K/Akt/FoxO3 Pathway in C2C12 Myotubes.","authors":"Yu Kyong Woo, Minseong Kang, Changhee Kim, Jae-Kwan Hwang","doi":"10.3746/pnf.2024.29.2.154","DOIUrl":"10.3746/pnf.2024.29.2.154","url":null,"abstract":"Skeletal muscle atrophy, which is characterized by diminished muscle mass, strength, and function, is caused by malnutrition, physical inactivity, aging, and diseases. Korean mint (Agastache rugosa Kuntze) possesses various biological functions, including anti-inflammatory, antioxidant, anticancer, and antiosteoporosis activities. Moreover, it contains tilianin, which is a glycosylated flavone that exerts antioxidant, anti-inflammatory, antidiabetic, and neuroprotective activities. However, no studies have analyzed the inhibitory activity of A. rugosa extract (ARE) and tilianin on muscle atrophy. Thus, the present study investigated the potential of ARE and tilianin on muscle atrophy and their underlying mechanisms of action in C2C12 myotubes treated with tumor necrosis factor-α (TNF-α). The results showed that ARE and tilianin promoted the phosphatidylinositol 3-kinase/protein kinase B pathway, thereby activating mammalian target of rapamycin (a protein anabolism-related factor) and its downstream factors. Moreover, ARE and tilianin inhibited the mRNA expression of muscle RING-finger protein-1 and atrogin-1 (protein catabolism-related factors) by blocking Forkhead box class O3 translocation. ARE and tilianin also mitigated inflammatory responses by downregulating nuclear factor-kappa B expression levels, thereby diminishing the expression levels of inflammatory cytokines, including TNF-α and interleukin-6. Additionally, ARE and tilianin enhanced the expression levels of antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. Overall, these results suggest that ARE and tilianin are potential functional ingredients for preventing or improving muscle atrophy.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"154-161"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Haskap (Lonicera caerulea L.) Extracts against Oxidative Stress and Inflammation in RAW 264.7 Cells. 哈斯卡普（忍冬）提取物对 RAW 264.7 细胞氧化应激和炎症的影响

IF 1.6

Preventive Nutrition and Food Science Pub Date : 2024-06-30 DOI: 10.3746/pnf.2024.29.2.146

Chong Li, Jeong Hyeon Kang, Kyung Im Jung, Mi Hwa Park, Mihyang Kim

{"title":"Effects of Haskap (Lonicera caerulea L.) Extracts against Oxidative Stress and Inflammation in RAW 264.7 Cells.","authors":"Chong Li, Jeong Hyeon Kang, Kyung Im Jung, Mi Hwa Park, Mihyang Kim","doi":"10.3746/pnf.2024.29.2.146","DOIUrl":"10.3746/pnf.2024.29.2.146","url":null,"abstract":"This study aimed to evaluate the antioxidant and anti-inflammatory activities of Lonicera caerulea L. ethanol extract (LCEE) and water extract (LCWE) in vitro. We primarily evaluated the improvement effect of LCWE and LCEE on hydrogen peroxide (H2O2)-induced oxidative damage and lipopolysaccharide (LPS)-induced inflammatory damage in RAW 264.7 cells by detecting oxidation-related indicators and inflammatory factors, respectively. Cellular studies showed that LCWE and LCEE increased superoxide dismutase and catalase antioxidant enzyme levels and decreased malondialdehyde and nitric oxide peroxide levels in H2O2-induced RAW 264.7 cells. Moreover, LCWE and LCEE decreased the secretion of inflammatory factors [e.g., interleukin (IL)-6, IL-1β, and tumor necrosis factor-α] in LPS-induced RAW 264.7 cells. In conclusion, LCWE and LCEE demonstrated excellent antioxidant and anti-inflammatory effects in vitro. However, LCWE was superior to LCEE, which may be related to its chemical composition and requires further research.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"29 2","pages":"146-153"},"PeriodicalIF":1.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harmine Hydrochloride Induces G2/M Cell Cycle Arrest and Apoptosis in SK-Hep1 Hepatocellular Carcinoma Cells by Regulating Mitogen-Activated Protein Kinases and the PI3K/AKT Pathway. 盐酸哈明通过调节丝裂原活化蛋白激酶和 PI3K/AKT 通路诱导 SK-Hep1 肝细胞癌细胞的 G2/M 细胞周期停滞和凋亡

Preventive Nutrition and Food Science Pub Date : 2023-12-31 DOI: 10.3746/pnf.2023.28.4.436

Gi Dae Kim

{"title":"Harmine Hydrochloride Induces G2/M Cell Cycle Arrest and Apoptosis in SK-Hep1 Hepatocellular Carcinoma Cells by Regulating Mitogen-Activated Protein Kinases and the PI3K/AKT Pathway.","authors":"Gi Dae Kim","doi":"10.3746/pnf.2023.28.4.436","DOIUrl":"10.3746/pnf.2023.28.4.436","url":null,"abstract":"Liver cancer is a globally common form of cancer. Thus, novel drugs derived from natural products are needed to reduce the side effects of chemotherapy. The present study aimed to analyze the anticancer properties and effects of harmine hydrochloride (HMH), a water-soluble metabolite of harmine that can be easily absorbed into tissues, in treating liver cancer cells. HMH dose-dependently inhibited cell growth, migration, invasion, and colony formation in SK-Hep1 cells. It also induced G2/M arrest by reducing the expression of p-cdc2, cyclin B1, and Rb (G2/M phase regulatory proteins) in a dose-dependent manner. HMH treatment reduced the expression of caspase-9, caspase-3, PARP, and Bcl-2 and increased the expression of Bax (a proapoptotic protein). Moreover, it increased the production of reactive oxygen species and decreased the intracellular uptake of rhodamine 123 due to mitochondrial dysfunction because of oxidative stress. HMH treatment also upregulated the phosphorylation of JNK, p38, and FOXO3a in SK-Hep1 cells and downregulated the PI3K/AKT signaling pathway. Our findings suggest that HMH may activate the compounds responsible for anticancer effects in hepatocellular carcinoma cells.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"28 4","pages":"436-443"},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10764232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139378196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0