Practical Laboratory Medicine最新文献

{"title":"An alternative method for inferring Pandy's test using cerebrospinal fluid total protein","authors":"Liu Dong ,&nbsp;Xiaoqing Wang ,&nbsp;Qianqian Xu,&nbsp;Ruoshui Cao,&nbsp;Xuan Deng,&nbsp;Jian Chen,&nbsp;Haoqin Jiang","doi":"10.1016/j.plabm.2024.e00411","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00411","url":null,"abstract":"<div><h3>Background</h3><p>Pandy's test is used to assess the globulin level in cerebrospinal fluid (CSF). As a semi-quantitative manual method, the practicality and clinical value of Pandy's test has been challenged.</p></div><div><h3>Objective</h3><p>We tend to summarize the relationship between CSF total protein (CSF-TP) quantification and Pandy's results, providing a formula to estimate Pandy's results merely by CSF-TP value.</p></div><div><h3>Methods</h3><p>This retrospective study involved 1090 cases hospitalized in Huashan Hospital during 1/1/2023 to 20/4/2023. All samples were divided into six group based on their Pandy's results. Their corresponding CSF-TP quantitative results were subsequently analyzed and summarized. Another 364 patients were also gathered for verification.</p></div><div><h3>Results</h3><p>The turbidity of samples won't affect examiners'ocular inspection and interpretation of Pandy's tests in positive groups. The results of Pandy's tests can be deduced based on CSF-TP quantitative results according to following rules: CSF-TP quantitative results 0–614 mg/L for Pandy negative (−), 615–1322 mg/L for extremely weak positive (±), 1323–2953 mg/L for weak positive (1+), 2954–6561 mg/L for medium positive results (2+), 6562–13007 mg/L for strong positive results (3+) and CSF-TP results &gt;13007 for strongest positive (4+). The quantitative range above was experimentally verified as effective and correct by calculating the agreement rate through another 364 samples and the R ratio of each Pandy group was greater than 90 %.</p></div><div><h3>Conclusion</h3><p>There is an excellent correlation between CSF-TP and Pandy's test. Therefore, CSF-TP quantification test through PROT Slides can be used to infer the results of Pandy's test to accelerate the abolish of this traditional manual test.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"40 ","pages":"Article e00411"},"PeriodicalIF":1.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S235255172400057X/pdfft?md5=b5b628c6d5255304cd0b92e6160d6cd3&pid=1-s2.0-S235255172400057X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141239037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refractory hypoxia and saturation gap in a COVID-19 patient","authors":"Abidah Mobarak ,&nbsp;Subashini C. Thambiah ,&nbsp;Ana Daliela Masiman ,&nbsp;Intan Nureslyna Samsudin ,&nbsp;Yin Ye Lai","doi":"10.1016/j.plabm.2024.e00395","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00395","url":null,"abstract":"<div><p>Acquired methemoglobinemia, predominantly due to oxidizing medications occurs when heme iron in hemoglobin is oxidized from ferrous to ferric ion and binds oxygen irreversibly leading to functional anemia, cyanosis, and tissue hypoxia. We report a case of a 60-year-old man with multiple comorbidities who was diagnosed with coronavirus disease 2019 (COVID-19) and developed methemoglobinemia after consumption of prescribed supplements. He presented with dyspnea and cyanosis. An oxygen saturation gap with characteristic chocolate-brown arterial blood indicated methemoglobinemia. Outsourced methemoglobin (MetHb) was increased at 9.0%. Despite aggressive intervention, he succumbed to his illness. In this case, we discuss the pathophysiology of why some individuals, especially the elderly with COVID-19 are more susceptible to develop methemoglobinemia after possibly being exposed to oxidizing agents. Laboratory methods for assessing oxygen saturation, including pulse oximetry, arterial blood gas and co-oximetry are examined in relation to this case. The importance of considering a diagnosis of methemoglobinemia based on clinical and biochemical findings although MetHb assay or co-oximetry are not readily available is also emphasized.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"40 ","pages":"Article e00395"},"PeriodicalIF":1.9,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000416/pdfft?md5=f4925cc54af639949a5b0292b7eee6dd&pid=1-s2.0-S2352551724000416-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140639349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a clamshell isothermal nucleic acid amplification analyzer in the detection of lower respiratory tract bacteria","authors":"Guanbin Zhang ,&nbsp;Xiaoying Lin ,&nbsp;Wenkun Mu ,&nbsp;Jun Luo ,&nbsp;Yiyuan Xu ,&nbsp;Chicheng Song ,&nbsp;Jiang Li","doi":"10.1016/j.plabm.2024.e00394","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00394","url":null,"abstract":"<div><h3>Objectives</h3><p>The clamshell isothermal nucleic acid amplification analyzer RTisochip-S, a next-generation instrument featuring improved structural design, enhanced functional integration, reduced cost, and increased portability, was assessed for its suitability in clinical respiratory pathogens detection.</p></div><div><h3>Methods</h3><p>The certificated detection kit for lower respiratory tract bacteria (LRTB-kit) was applied to evaluate the performance of RTisochip-S via sensitivity, specificity, and repeatability analysis. The clinical specimens, including 51 sputum specimens and 10 bronchoalveolar lavage fluid specimens, were simultaneously detected on both RTisochip-S and a certificated reference instrument (RTisochip-A) to assess the consistency.</p></div><div><h3>Results</h3><p>The results indicated that RTisochip-S fulfills the sensitivity, specificity, and repeatability requirements of the LRTB-Kit, and the results of clinical specimens on the two instruments were consistent.</p></div><div><h3>Conclusions</h3><p>RTisochip-S is satisfying the clinical detection of respiratory pathogens while enhancing portability and compactness, making it more well-suited for point-of-care testing (POCT) applications.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"40 ","pages":"Article e00394"},"PeriodicalIF":1.9,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000404/pdfft?md5=cf27d7a272776a666a30c260483b2917&pid=1-s2.0-S2352551724000404-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140618087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of salivary cortisol measurements using different assay methods in relation to serum-free cortisol measurement","authors":"Anna Lee ,&nbsp;Sooah Jang ,&nbsp;Sanghoo Lee ,&nbsp;Hyun-Kyung Park ,&nbsp;In-Young Kim ,&nbsp;Ryunsup Ahn ,&nbsp;Jeong-Ho Seok ,&nbsp;Kyoung-Ryul Lee","doi":"10.1016/j.plabm.2024.e00393","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00393","url":null,"abstract":"<div><h3>Objectives</h3><p>Salivary cortisol reflects the biologically active form of serum cortisol, offering a noninvasive evaluation method for the diurnal rhythm of the hypothalamic-pituitary-adrenal (HPA) axis. While liquid chromatography-tandem mass spectrometry (LC-MS/MS) is known for its specificity, immunoassays (IA) are commonly used because of their simplicity. This study aimed to assess the performance of salivary cortisol measurement using both IA and LC-MS/MS in comparison to serum-free cortisol measurement.</p></div><div><h3>Methods</h3><p>Assay results for 188 saliva and 94 serum samples from 47 participants were analyzed. Salivary samples collected at different time points were analyzed using IA and LC-MS/MS. Serum samples were analyzed for cortisol, cortisol-binding globulin, and free cortisol. The statistical analyses included correlations and method comparisons.</p></div><div><h3>Results</h3><p>The diurnal salivary cortisol profiles exhibited a comparable circadian rhythm pattern; however, the concentrations measured using IA were consistently higher than those measured using LC-MS/MS. The correlation analysis revealed robust associations among salivary cortisol (IA), salivary cortisol (LC-MS/MS), and serum-free cortisol levels (LC-MS/MS). However, the method comparison revealed a systematic bias between IA and LC-MS/MS in salivary cortisol measurement.</p></div><div><h3>Conclusions</h3><p>This study contributes to the ongoing debate on assay techniques by affirming the suitability of IA and LC-MS/MS for salivary cortisol measurement to assess dynamic changes in HPA axis activity. The identified systematic bias emphasizes the importance of selecting methods based on specific research or clinical requirements.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"40 ","pages":"Article e00393"},"PeriodicalIF":1.9,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000398/pdfft?md5=89279cbc1bebd280b01274033e305a55&pid=1-s2.0-S2352551724000398-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140549343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy","authors":"Sedat Abusoglu ,&nbsp;Halil Guven ,&nbsp;Busra Ecer ,&nbsp;Ahmet Emre Yorulmaz ,&nbsp;Abdullah Sivrikaya ,&nbsp;Fatma Humeyra Yerlikaya Aydemir ,&nbsp;Ali Unlu ,&nbsp;Gulsum Abusoglu ,&nbsp;Muhittin Abdulkadir Serdar","doi":"10.1016/j.plabm.2024.e00386","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00386","url":null,"abstract":"<div><h3>Objectives</h3><p>Urinalysis is a first-line test for screening for urinary tract infection. Several devices performing strip and sediment analysis have been introduced. The aim of this study was to compare the performance of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy.</p></div><div><h3>Methods</h3><p>463 urine samples were analyzed. Digital image processing and particle recognition automatically display the cells in a flowing sheath fluid mixed monolayer urine sample, take the pictures of particles via digital camera, analyse these pics with a particle recognition software, transfer images of the formed elements to the screen and allow well-trained personnel to select, reclassify or remove them. Manual microscopy was used for comparison.</p></div><div><h3>Results</h3><p>Agreement between Tricell-100 and manual microscopy was very good for RBC (ϰ = 0.80), and WBC (ϰ = 0.83); good for CaOx (ϰ = 0.69), SEC (ϰ = 0.80), YLC (ϰ = 0.72), HC (0.69) and LC (ϰ = 0.64); moderate for BAC (ϰ = 0.51), APC (ϰ = 0.43) and MT (ϰ = 0.55); fair for GC (ϰ = 0.39) and RTEC (ϰ = 0.32).</p></div><div><h3>Conclusions</h3><p>Labsan Trion TriCell-1000 demonstrated satisfactory performance and can be used in routine urinalysis. In the case of low counts of RBC, presence of yeast, crystal, casts or cell clumping in urine sediment, characterization of urine particles should be performed by manual microscopy.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00386"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000325/pdfft?md5=7f05a63231e96c752eb99bf724ad7d26&pid=1-s2.0-S2352551724000325-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140015837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Added value of a connected glucose meter for glycorrhachia assessment","authors":"Laurent Blairon ,&nbsp;Marie Tré-Hardy ,&nbsp;Sophie Collignon ,&nbsp;François Coenen ,&nbsp;Ingrid Beukinga ,&nbsp;Roberto Cupaiolo","doi":"10.1016/j.plabm.2024.e00384","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00384","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer.</p></div><div><h3>Design and Methods</h3><p>Intra-assay and inter-assay accuracies were calculated using residual clinical samples. Accuracies were measured by comparing the results obtained with the glucometer to those from the central laboratory on a large routine chemistry platform.</p></div><div><h3>Results</h3><p>The intra-assay coefficients of variation were between 6.1% and 6.2% for low values (18 mg/dL) and between 5.6% and 6.8% for high values (58 mg/dL). The inter-assay coefficients of variation were between 9.4% and 16.3% for the low values (18 mg/dL) and between 5.7% and 8.7% for the high values (pool; ±75 mg/dL). The regression equation by comparison to the central laboratory was y = 4.08 + 0.82 x, with a coefficient of determination (r²) of 0.95.</p></div><div><h3>Conclusions</h3><p>The measurement of glycorrhachia with a connected glucometer before the analysis in the central laboratory allows a rapid orientation in the deferential diagnosis of a meningitis of viral vs bacterial origin. The response time is fast (6 s) and requires only a small amount of fluid (1.2 μL), which is important in infants, especially since lumbar puncture is an integral part of the investigation of the origin of a fever in this population.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00384"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000301/pdfft?md5=832c69b64e8568ee7d0ec1e53b2808dd&pid=1-s2.0-S2352551724000301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140031400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of plasma collection tubes on N-glycome in human blood samples","authors":"Zejian Zhang ,&nbsp;Xiangyi Cui ,&nbsp;Nan Zhou ,&nbsp;Lisi Zhu ,&nbsp;Yuxiang Zhi ,&nbsp;Shuyang Zhang","doi":"10.1016/j.plabm.2024.e00383","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00383","url":null,"abstract":"<div><h3>Background and aims</h3><p>Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.</p></div><div><h3>Materials and methods</h3><p>We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.</p></div><div><h3>Results</h3><p>Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits.</p></div><div><h3>Conclusion</h3><p>Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00383"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000295/pdfft?md5=2f804ffd65e96a5c5246b7e8c695a672&pid=1-s2.0-S2352551724000295-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of chemiluminescent immunoassay and enzyme-linked immunosorbent assay for detecting phospholipase A2 receptor antibody in primary membranous nephropathy","authors":"Xiaotao Ma ,&nbsp;Ruiting Wang ,&nbsp;Linting Wei ,&nbsp;Pengfei Liu ,&nbsp;Lanmei Jing ,&nbsp;Jinghua Wang ,&nbsp;Wei Dong ,&nbsp;Xuefei Tian ,&nbsp;Rongguo Fu","doi":"10.1016/j.plabm.2024.e00385","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00385","url":null,"abstract":"<div><h3>Objective</h3><p>The accurate detection of phospholipase A2 receptor (PLA2R) autoantibody is crucial in the diagnosis and monitoring of primary membranous nephropathy (pMN). While enzyme-linked immunosorbent assay (ELISA) is the commonly used detection method, its complexity and time-consuming nature pose challenges, especially for small sample sizes. Chemiluminescence immunoassay (CLIA) has emerged as a rapid alternative for clinical immunoassays. This study aims to compare the sensitivity, specificity, and precision of CLIA and ELISA in detecting PLA2R autoantibody.</p></div><div><h3>Method</h3><p>A total of 145 patients with biopsy-confirmed primary membranous nephropathy and 85 patients with non-membranous nephropathy were enrolled in this comparative study. CLIA and ELISA were employed to test all samples for the presence of PLA2R autoantibodies. Statistical analysis of sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) was performed using SPSS 26.0. The diagnostic value of ELISA and CLIA for pMN was analyzed using the ROC curve, and Correlation analysis was performed using Spearman.</p></div><div><h3>Results</h3><p>Serum levels of anti-PLA2R antibody in pMN group were significantly higher than those in nMN group(P &lt; 0.05). The accuracy of CLIA for detecting anti-PLA2R antibody was 76.96%, while ELISA showed an accuracy of 74.78%. The sensitivity for CLIA was 64.83%, compared to 60% for ELISA. However, no statistically significant difference was observed between the two methods (<em>P</em> &gt; 0.05). The overall qualitative agreement of anti-PLA2R detection was 93.35% (95% confidence interval[CI] 89.47–96.3). ROC curve analysis showed that AUC of anti-PLA2R antibody detected by ELISA and CLIA were 0.8737 (95% confidence interval [CI] 0.8270–0.9204), 0.8914 (95% confidence interval [CI]0.8495–0.9332), respectively. The Spearman correlation analysis revealed a significant correlation between them(<em>P</em> &lt; 0.05). Notably, CLIA demonstrated a significant time-saving advantage, particularly when the sample size was less than 200, and especially when it was less than 20.</p></div><div><h3>Conclusion</h3><p>CLIA and ELISA showed similar accuracy and consistency in detecting anti-PLA2R antibody for primary membranous nephropathy. However, CLIA exhibited a significant advantage in terms of automation and time-saving compared to ELISA, particularly for smaller sample sizes. This finding suggests that CLIA has the potential to become a preferred and widely adopted test in the future.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00385"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000313/pdfft?md5=77ed71aee7e179b45c671f2edfe84687&pid=1-s2.0-S2352551724000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between locally versus centrally processed serum procalcitonin during emergency department research evaluation of febrile infants aged 0–60 days","authors":"Cosby G. Arnold ,&nbsp;Prashant Mahajan ,&nbsp;Russell K. Banks ,&nbsp;John M. VanBuren ,&nbsp;Nam K. Tran ,&nbsp;Octavio Ramilo ,&nbsp;Nathan Kuppermann","doi":"10.1016/j.plabm.2024.e00391","DOIUrl":"10.1016/j.plabm.2024.e00391","url":null,"abstract":"<div><h3>Introduction</h3><p>Procalcitonin (PCT) is a useful biomarker in the initial evaluation of febrile infants for serious bacterial infections (SBIs). However, PCT is not always available locally and must at times be frozen and shipped to a reference laboratory for research studies. We sought to compare PCT measured locally versus centrally at a reference laboratory during a research study.</p></div><div><h3>Materials and methods</h3><p>This was a secondary analysis of a multicenter study of febrile infants ≤60 days evaluated for SBIs from June 2016 to April 2019. A PCT cutoff value of 0.5 ng/mL was used to stratify infants at low-versus high-risk of SBIs. Statistical analyses consisted of Spearman's correlation, Bland-Altman difference plotting, Passing-Bablok regression, Deming regression, and Fisher's exact testing at the 0.5 ng/mL threshold.</p></div><div><h3>Results</h3><p>241 febrile infants had PCT levels measured both locally and at the reference laboratory. PCT levels measured locally on 5 different platforms and from the frozen research samples demonstrated strong Spearman's correlation (ρ = 0.83) and had similar mean PCT values with an average relative difference of 0.02%. Eleven infants with SBIs had PCT values &lt; 0.5 ng/mL in both the clinical and research samples. Six other infants had differences in SBI prediction based on PCT values at the 0.5 ng/mL threshold between the clinical and research platforms.</p></div><div><h3>Conclusions</h3><p>We found no significant differences in detection of febrile infants at high risk for SBI based on locally (on multiple platforms) versus centrally processed PCT. Testing at a central reference laboratory after freezing and shipping is an accurate and reliable alternative for research studies or when rapid turnaround is not required.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00391"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000374/pdfft?md5=4f51ab267812173286559ea7c32b5f65&pid=1-s2.0-S2352551724000374-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140275291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are we ready for widespread implementation of lactate POCT in fetal blood sampling? An analytical and clinical verification of the novel statstrip POCT analyzer","authors":"Nienke Spronk ,&nbsp;Madeleine SQ. Kortenhorst ,&nbsp;Jasmijn A. van Balveren","doi":"10.1016/j.plabm.2024.e00375","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00375","url":null,"abstract":"<div><h3>Objectives</h3><p>Currently, pH values are used in fetal scalp blood sampling as a parameter to rule out fetal distress during the delivery. Due to a high number of pre-analytical errors in pH measurements, lactate measurement is already extensively examined as an alternative. Our objectives were to confirm the analytical performance of the StatStrip lactate POCT analyzer and to compare pH and lactate as a marker of fetal distress.</p></div><div><h3>Design</h3><p><em>and methods</em>: Fetal blood scalp, umbilical and arterial blood test results (n = 100) were analyzed to compare the current POC blood gas analyzer including lactate (iSTAT-1) with the new POCT analyzer (StatStrip) to test the analytical performance. Furthermore, in all fetal scalp blood tests with a lactate en pH measurement from 2021 to 2023, clinical delivery data was collected to perform a clinical verification.</p></div><div><h3>Results</h3><p>The lactate concentration on the StatStrip analyzer correlated well with the iSTAT-1 (Pearson's r ≥ 0.95). 73 Fetal scalp blood tests showed 18% discrepant results when comparing pH and lactate with regard to fetal distress and consequent delivery intervention. Lactate showed more false positive results than pH (4 versus 1), but no false negatives as opposed to pH (0 versus 1).</p></div><div><h3>Conclusions</h3><p>The lactate Statstrip and iSTAT-1 POCT analyzers were analytically equivalent. The clinical verification study showed that lactate is a good predictor of fetal distress, although more false positive results were found in our limited dataset. However, unnecessary interventions due to failed pH measurements might be prevented when a lactate measurement is introduced.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00375"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000210/pdfft?md5=264c8d80341769c182b509e0f7e6321b&pid=1-s2.0-S2352551724000210-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140160673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}