An alternative method for inferring Pandy's test using cerebrospinal fluid total protein

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY
Liu Dong , Xiaoqing Wang , Qianqian Xu, Ruoshui Cao, Xuan Deng, Jian Chen, Haoqin Jiang
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引用次数: 0

Abstract

Background

Pandy's test is used to assess the globulin level in cerebrospinal fluid (CSF). As a semi-quantitative manual method, the practicality and clinical value of Pandy's test has been challenged.

Objective

We tend to summarize the relationship between CSF total protein (CSF-TP) quantification and Pandy's results, providing a formula to estimate Pandy's results merely by CSF-TP value.

Methods

This retrospective study involved 1090 cases hospitalized in Huashan Hospital during 1/1/2023 to 20/4/2023. All samples were divided into six group based on their Pandy's results. Their corresponding CSF-TP quantitative results were subsequently analyzed and summarized. Another 364 patients were also gathered for verification.

Results

The turbidity of samples won't affect examiners'ocular inspection and interpretation of Pandy's tests in positive groups. The results of Pandy's tests can be deduced based on CSF-TP quantitative results according to following rules: CSF-TP quantitative results 0–614 mg/L for Pandy negative (−), 615–1322 mg/L for extremely weak positive (±), 1323–2953 mg/L for weak positive (1+), 2954–6561 mg/L for medium positive results (2+), 6562–13007 mg/L for strong positive results (3+) and CSF-TP results >13007 for strongest positive (4+). The quantitative range above was experimentally verified as effective and correct by calculating the agreement rate through another 364 samples and the R ratio of each Pandy group was greater than 90 %.

Conclusion

There is an excellent correlation between CSF-TP and Pandy's test. Therefore, CSF-TP quantification test through PROT Slides can be used to infer the results of Pandy's test to accelerate the abolish of this traditional manual test.

利用脑脊液总蛋白推断潘迪试验的另一种方法
背景潘迪试验用于评估脑脊液(CSF)中的球蛋白水平。方法本回顾性研究涉及华山医院 2023 年 1 月 1 日至 2023 年 4 月 20 日住院的 1090 例患者。所有样本根据潘迪结果分为六组。随后对其相应的 CSF-TP 定量结果进行分析和总结。结果在阳性组中,样本的浑浊度不会影响检查人员的眼部检查和对 Pandy's 检测结果的解读。根据 CSF-TP 定量结果,可按以下规则推断 Pandy's 试验的结果:CSF-TP 定量结果 0-614 mg/L 为潘迪阴性(-),615-1322 mg/L 为极弱阳性(±),1323-2953 mg/L 为弱阳性(1+),2954-6561 mg/L 为中等阳性(2+),6562-13007 mg/L 为强阳性(3+),CSF-TP 结果 >13007 为最强阳性(4+)。通过计算另外 364 份样本的一致率,实验验证了上述定量范围的有效性和正确性,各 Pandy 组的 R 比值均大于 90%。因此,通过 PROT 幻灯片进行 CSF-TP 定量试验可用于推断 Pandy 试验的结果,从而加快取消这种传统的人工试验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Practical Laboratory Medicine
Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology
CiteScore
3.50
自引率
0.00%
发文量
40
审稿时长
7 weeks
期刊介绍: Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.
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