Practical Laboratory Medicine最新文献

{"title":"Corrigendum to “Comparative analysis of diagnostic kits for detecting Ureaplasma spp. and M. hominis in genital and urinary samples” [Pract Lab Med, 49 (2026) 525]","authors":"Juliette Beaucarne, Ivana Buttice, Nada Riahi, Ethel Seyll, Mony Hing, Bram Vanmechelen, Delphine Martiny","doi":"10.1016/j.plabm.2026.e00545","DOIUrl":"10.1016/j.plabm.2026.e00545","url":null,"abstract":"","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"51 ","pages":"Article e00545"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct rapid antimicrobial susceptibility testing (RAST) from positive blood cultures: Recent advances, challenges, and future directions","authors":"Haiyi Li,&nbsp;Qiongfang Zhu,&nbsp;Yalu Ren,&nbsp;Meili Shen,&nbsp;Jie Xu","doi":"10.1016/j.plabm.2026.e00555","DOIUrl":"10.1016/j.plabm.2026.e00555","url":null,"abstract":"<div><div>Bloodstream infections (BSIs) carry high morbidity and mortality, and delayed targeted therapy worsens survival. Conventional antimicrobial susceptibility testing (AST) from positive blood cultures typically requires 24–48 h (and &gt;72 h for some resistant organisms), delaying appropriate treatment and leaving 14–78% of empirical regimens unnecessary or ineffective. Direct rapid antimicrobial susceptibility testing (RAST) addresses this bottleneck by delivering AST in 4–16 h with categorical agreement (CA) ≥90% for major pathogens, using volatile organic compound (VOC) detection, modified disk diffusion, microfluidics, and molecular approaches. RAST generally makes susceptibility results available approximately 8–44 h earlier, with savings exceeding 56 h when conventional testing requires more than 72 h. This review compares the principles, turnaround times (TAT), and performance of leading RAST platforms. It further identifies persistent bottlenecks restricting cross-platform comparability and clinical uptake, most notably the absence of standardized protocols, the inability of molecular assays to determine minimum inhibitory concentrations (MICs), and unresolved pre-analytical confounders. Although RAST reliably shortens time-to-result and supports antimicrobial stewardship, its routine integration demands platform-specific validation, transparent regulatory and cost-effectiveness assessment.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"51 ","pages":"Article e00555"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Maternal vaginal colonization screening for term singleton pregnancy: comparative evaluation of metagenomic next-generation sequencing (mNGS) versus real-time quantitative PCR (qPCR)” [Pract. Lab. Med. (2026) 542]","authors":"Jinyu Zhang,&nbsp;Jingyi Liang,&nbsp;Fei Lv,&nbsp;Zhusheng Guo","doi":"10.1016/j.plabm.2026.e00552","DOIUrl":"10.1016/j.plabm.2026.e00552","url":null,"abstract":"","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"51 ","pages":"Article e00552"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of free light chain assays in AL amyloidosis: Performance of Sebia versus Freelite and N-Latex","authors":"Frances Zhao ,&nbsp;Carel Pretorius ,&nbsp;Peter Mollee","doi":"10.1016/j.plabm.2026.e00519","DOIUrl":"10.1016/j.plabm.2026.e00519","url":null,"abstract":"","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"49 ","pages":"Article e00519"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Truenat® chip-based real-time PCR platform for infectious disease diagnostics","authors":"João Renato Rebello Pinho ,&nbsp;Gabriela Rampazzo Cruz ,&nbsp;Priscila de Meira Oliveira ,&nbsp;Fernanda de Mello Malta ,&nbsp;Roberta Cardoso Petroni ,&nbsp;Roberta Miraglia ,&nbsp;Ana Claudia Campana ,&nbsp;Alice Friedenberg de Ulhoa Cintra ,&nbsp;Ricardo Andreotti Siqueira ,&nbsp;Rubia Anita Ferraz Santana ,&nbsp;Fabiane Camargo Gomes Nunes ,&nbsp;Marinês Dalla Valle Martino ,&nbsp;André Mario Doi ,&nbsp;Nair Hideko Muto ,&nbsp;Cristóvão Luis Pitangueira Mangueira","doi":"10.1016/j.plabm.2026.e00522","DOIUrl":"10.1016/j.plabm.2026.e00522","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the Trueprep/Truelab platform with four Truenat assays (COVID-19, <em>Mycobacterium tuberculosis</em> - MTB, malaria, leptospirosis), focusing on analytical performance, repeat rates, and time-to-result.</div></div><div><h3>Methods</h3><div>Remnant clinical specimens and commercial controls were analyzed under routine conditions. Performance metrics included accuracy, reproducibility, limit of detection (LOD), linearity, and specificity, when applicable. Each analyte was tested in replicates, and results were compared with established reference methods.</div></div><div><h3>Results</h3><div>The Truenat COVID-19 assay achieved 100% agreement with the reference and LOD of 500 copies/mL. Truenat MTB showed 95% concordance with the comparator, though repeat runs were occasionally required (7.3%). Malaria assays demonstrated high reproducibility and linearity (R² = 0.9996) with an observed LOD of 3000 copies/mL. Leptospira assays yielded 100% accuracy with a LOD of 750 copies/mL. Performance generally matched manufacturer specifications, though low-burden MTB samples were less consistently detected.</div></div><div><h3>Conclusion</h3><div>Truenat assays showed reliable analytical performance, especially for COVID-19. While MTB detection remains more robust with Xpert Ultra in low-burden cases, Truenat provides a viable point-of-care alternative in resource-limited settings. For malaria and leptospirosis, broader clinical validation is needed before routine implementation in our service.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"49 ","pages":"Article e00522"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146190342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic investigation and data-driven correction of lipemic interference in hematological parameters on the Mindray BC-6800 hematology analyzer","authors":"Shoumin Li ,&nbsp;Haiping Yang ,&nbsp;Qian Zhang ,&nbsp;Fu Zhao ,&nbsp;Yong Mu ,&nbsp;Yingjiang Chen","doi":"10.1016/j.plabm.2026.e00521","DOIUrl":"10.1016/j.plabm.2026.e00521","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to systematically investigate the interference of lipemia on the Mindray BC-6800 hematology analyzer, elucidate the underlying mechanisms via optical signal analysis, and develop data-driven strategies for its recognition and correction.</div></div><div><h3>Methods</h3><div>In vitro models of moderate and severe lipemia were established using 30 healthy specimens. Complete blood count (CBC) parameters, leukocyte differentials, and optical signals were measured and compared against controls. A random forest model was constructed for interference identification, and a multiple linear regression model was developed from 600 normal samples to predict hemoglobin (HGB).</div></div><div><h3>Results</h3><div>Lipemia significantly increased Hb, MCH, and MCHC (P &lt; 0.05), with severe lipemia further affecting HCT, LYMPH%, and MONO%. Optical analysis revealed increased X-axis (side scatter) and decreased Y-/Z-axis (fluorescence) signals (P &lt; 0.001). The random forest model achieved an AUC of 0.952, and a simplified rule (“MCH &gt;36 pg &amp; MCHC &gt;410 g/L″) attained 81.1% accuracy. The HGB prediction model (HGB = 6.67 + 3.14 × HCT) performed robustly across lipemia levels (R² &gt; 0.96).</div></div><div><h3>Conclusions</h3><div>Lipemia interferes with multiple parameters on the Mindray BC-6800 through alterations in cellular optical signals. The developed recognition and correction models demonstrate high clinical applicability for improving result accuracy.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"49 ","pages":"Article e00521"},"PeriodicalIF":1.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146190341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UF-5000 Atyp.C parameter for urinary screening: Impact of detecting atypical and inclusion-bearing cells","authors":"Yuki Fujiwara ,&nbsp;Yuzuru Takei ,&nbsp;Hiroto Nakazawa","doi":"10.1016/j.plabm.2026.e00520","DOIUrl":"10.1016/j.plabm.2026.e00520","url":null,"abstract":"<div><div>Urine sediment examination is vital for detecting atypical urothelial cells but is highly operator-dependent. The UF-5000 automated urine analyzer quantifies nucleic acid-containing particles. The atypical cell (Atyp.C) parameter reflects increased nucleic acid content and detects atypical urothelial cells and intracytoplasmic inclusion-bearing (ICIB) cells linked to viral infection or inflammation. We assessed the relationship between Atyp.C, atypical cells, and ICIBs and evaluated the parameter's screening performance. Overall, 264 urine sediment samples from 203 patients were analyzed using the UF-5000. Manual microscopy was used to identify atypical and ICIB-positive cells. Atyp.C values were compared between groups using the Mann–Whitney <em>U</em> test. Diagnostic performance was assessed using receiver operating characteristic (ROC) analysis and sensitivity, specificity, and Cohen's κ calculation at cutoffs of 0.1, 0.3, and 0.5 cells/μL. Atyp.C values were significantly higher in atypical-cell-positive specimens (<em>p &lt;</em> 0.001). Atypical cell and ICIB positivity showed moderate agreement (Cohen's κ = 0.534; 77 % agreement; <em>p &lt;</em> 0.01). ROC analysis showed an area under the curve of 0.829 for atypical cells, which increased to 0.895 when ICIB-positive samples were considered positive. At a cutoff of 0.1 cells/μL, Atyp.C exhibited high sensitivity (97.8 %) with low specificity (46.5 %) for detecting atypical cells; a 0.3 cells/μL cutoff provided optimal balance (sensitivity 85.2 %, specificity 68.2 %). The UF-5000 Atyp.C parameter effectively detects cells with increased nucleic acid content, including atypical and ICIB-positive cells. Recognizing ICIBs as diagnostically relevant improves screening sensitivity, supporting Atyp.C as a valuable tool for urinalysis. Combining automated detection with manual microscopy may improve the efficiency of atypical cell detection.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"48 ","pages":"Article e00520"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case report of an unconventional chronic lead poisoning resulting from daily eyelid application of kohl","authors":"Charlène Aïn ,&nbsp;Antoine Baudriller ,&nbsp;Olivier Mathieu ,&nbsp;Yoann Cazaubon","doi":"10.1016/j.plabm.2025.e00517","DOIUrl":"10.1016/j.plabm.2025.e00517","url":null,"abstract":"<div><h3>Introduction</h3><div>Lead can be found everywhere in the environment and continues to be a public health concern. Lead exposure through kohl is a well-identified problem. In adults, the few reported cases show that this exposure is underestimated. The health consequences of chronic exposure are not well known.</div></div><div><h3>Case report</h3><div>a 63-year-old woman contacted the Department of Medical Pharmacology and Toxicology to report suffering from numerous neurological disorders. She mentioned having previously undergone toxicological assessments showing abnormally high levels of lead and mercury about three years ago. Several lead tests revealed active lead exposure but at levels considered subtoxic, 170–198 μg/L. Subsequently, several interviews with an internist and a toxicologist allowed the identification of the contamination source: kohl. After discontinuing the use of kohl, it took a year for lead concentrations to drop below 50 μg/L. Apart from neurological symptoms, neither renal function nor hematopoiesis was affected.</div></div><div><h3>Discussion</h3><div>This is the first case report describing the effects of chronic lead poisoning in adults over a period of more than 40 years. It is crucial to raise awareness among populations about daily using traditional kohls and to avoid these products due to their manufacturing process using galena.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"48 ","pages":"Article e00517"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of two different blood collection tubes for hematological and biochemical testing","authors":"Michela Salvatici ,&nbsp;Francesca Carreras ,&nbsp;Monica Gaimarri ,&nbsp;Francesca Delia Sansico ,&nbsp;Paolo Marinoni ,&nbsp;Chiara Masserini ,&nbsp;Barbara Bianchi ,&nbsp;Carmen Sommese ,&nbsp;Lorenzo Drago","doi":"10.1016/j.plabm.2025.e00515","DOIUrl":"10.1016/j.plabm.2025.e00515","url":null,"abstract":"<div><h3>Background</h3><div>Blood collection tubes can influence sample stability and analytical accuracy. We compared two different commercially available tube systems, Vacutainer® (BD) and Vacusera® (Disera), assessing stability of hematological and biochemical parameters under various preanalytical conditions.</div></div><div><h3>Methods</h3><div>Residual blood samples from routine laboratory testing were aliquoted into both different tubes. Parameters measured included Red blood cells (RBC), White blood cells (WBC), Hemoglobin (Hb), Potassium (K), Chloride (Cl), Sodium (Na), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH), along with hemolysis index. Preanalytical variables included storage temperature (ambient vs 4 °C), storage time (T₀, 1h, 3h, 24h), and transport conditions (local vs remote collection sites, with/without pre-centrifugation).</div></div><div><h3>Results</h3><div>From January 27 to July 31, 2025, 95 samples were analyzed: 49 from the Castellanza Hospital site, located approximately 50 km from the main laboratory (26 stored at room temperature, 23 at 4 °C), and 46 from the Fantoli MultiLab Laboratory (25 samples at room temperature, 21 at 4 °C), yielding 6621 determinations. Comparative analysis demonstrated no clinically significant differences between the two tube types under all tested conditions. Minor variations in K, Na and LDH (Castellanza Hospital site), Hb and ALT (Fantoli MultiLab Laboratory) were within acceptable analytical variation. Hemolysis index remained comparable between tubes in all scenarios, including transport and delayed processing.</div></div><div><h3>Conclusion</h3><div>Vacutainer® (BD) and Vacusera® (Disera) tube systems showed analytical equivalence across hematology and biochemistry parameters under multiple preanalytical conditions. We conclude that the tubes are suitable for common clinical hematological use and show acceptable performance for common clinical chemistry parameters.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"48 ","pages":"Article e00515"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the stability of 20 biochemical analytes in serum and whole blood samples after storage at nonstandard temperatures","authors":"Gabriella Iacovetti ,&nbsp;Bradley B. Collier ,&nbsp;Jill M. Rafalko ,&nbsp;Mitchell Peevler ,&nbsp;Nicolas Tokunaga ,&nbsp;Jason Ragar ,&nbsp;Whitney C. Brandon ,&nbsp;Matthew R. Chappell ,&nbsp;Russell P. Grant ,&nbsp;Greg J. Sommer ,&nbsp;Ulrich Y. Schaff","doi":"10.1016/j.plabm.2025.e00514","DOIUrl":"10.1016/j.plabm.2025.e00514","url":null,"abstract":"<div><h3>Background</h3><div>Blood specimen transport conditions can have critical impacts on analyte stability and test result accuracy. This study evaluated the impact of multiday storage of specimens at 30 °C and 0 °C, approximating shipment under summer conditions and shipment in contact with melting ice, respectively.</div></div><div><h3>Methods</h3><div>Blood samples from 16 healthy subjects were processed as serum samples and as clotted whole blood (uncentrifuged) stored at 30 °C on separator gel, and as serum stored in microtainers at 0 °C, each for 24- and 72-h periods prior to analysis. Each sample was analyzed for 20 common analytes and compared to the values from a paired baseline control sample. Mean absolute and/or relative biases were calculated and compared to CLIA acceptance limits.</div></div><div><h3>Results</h3><div>Serum samples stored at 30 °C for 24- or 72-h met acceptance criteria for all assessed analytes, except for 72-h ALT, Total Bilirubin, Carbon Dioxide, Creatinine, and Sodium. Numerous analytes were unstable at 30 °C in uncentrifuged whole blood, with only Albumin, ALP, Total Bilirubin, Cholesterol, HDL, Total Protein, Triglycerides, Uric Acid, and hsCRP remaining stable up to 72 h. For serum samples stored at 0 °C, all 24- hour and 72-h analytes showed biases within limits.</div></div><div><h3>Conclusion</h3><div>Storage of serum samples at 0 °C for up to 72 h yielded valid results for all analytes studied. Storage at 30 °C for up to 72-h yielded valid results for most analytes when combined with centrifugation prior to storage. Compared to room temperature storage, ALT and Sodium in serum and whole blood showed signs of accelerated degradation at 30 °C.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"48 ","pages":"Article e00514"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145760774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}