Practical Laboratory Medicine最新文献

{"title":"Comparative evaluation of four new immunoassays and LC-MS/MS for the measurement of urinary free cortisol in Cushing's syndrome diagnosis","authors":"Qi Zhang ,&nbsp;Danni Mu ,&nbsp;Yichen Ma ,&nbsp;Yuemeng Li ,&nbsp;Yumeng Gao ,&nbsp;Yingying Hu ,&nbsp;Kui Zhang ,&nbsp;Fang Zhao ,&nbsp;Ran Gao ,&nbsp;Liangyu Xia ,&nbsp;Huijuan Zhu ,&nbsp;Songlin Yu ,&nbsp;Ling Qiu ,&nbsp;Xinqi Cheng","doi":"10.1016/j.plabm.2025.e00484","DOIUrl":"10.1016/j.plabm.2025.e00484","url":null,"abstract":"<div><h3>Objectives</h3><div>Twenty-four-hour urinary free cortisol (UFC) measurement is the initial diagnostic test for Cushing's syndrome (CS). We compared UFC determination by four new immunoassays using Autobio A6200, Mindray CL-1200i, Snibe MAGLUMI X8 and Roche 8000 e801 with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, we evaluated the value of 24-h UFC measured by four direct immunoassays for diagnosing CS.</div></div><div><h3>Methods</h3><div>Residual 24-hr urine samples of 94 CS and 243 non-CS patients collected from previous cohort were used. A laboratory-developed LC-MS/MS method was used as reference. UFC was measured by immunoassays using Autobio, Mindray, Snibe and Roche platforms. Method was compared using Passing–Bablok regression and Bland–Altman plot analyses. Cut-off values for each assay and corresponding sensitivities and specificities were calculated by ROC analysis.</div></div><div><h3>Results</h3><div>All four immunoassays showed strong correlations with LC-MS/MS (Spearman coefficient r = 0.950, 0.998, 0.967, and 0.951, respectively). All immunoassays showed proportionally positive bias. The areas under the curve were 0.953 for Autobio, 0.969 for Mindray, 0.963 for Snibe, and 0.958 for Roche. The cut-off values varied from 178.5 to 272.0 nmol/24 h). Assay sensitivity and specificity ranged from 89.66 % to 93.10 % and from 93.33 % to 96.67 %, respectively.</div></div><div><h3>Conclusions</h3><div>Four newly available direct immunoassays for measuring UFC show good analytical consistency compared to LC-MS/MS. The elimination of organic solvent extraction simplifies workflows while maintaining high diagnostic accuracy. Additionally, they exhibited similarly high diagnostic accuracy for CS identification. Future multi-center studies are needed to validate our findings and establish method-specific UFC cut-off values to enhance clinical utility.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"46 ","pages":"Article e00484"},"PeriodicalIF":1.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance evaluation of the Cobas c 703 analytical unit as part of Cobas Pro integrated solutions using LED as the sole photometric light source","authors":"Peter Findeisen ,&nbsp;Inger Brandt ,&nbsp;Frederic Winnock ,&nbsp;Jan Furrer ,&nbsp;Kai Klopprogge","doi":"10.1016/j.plabm.2025.e00482","DOIUrl":"10.1016/j.plabm.2025.e00482","url":null,"abstract":"<div><h3>Background</h3><div>Most commercially available clinical chemistry systems today rely on a halogen light source to power photometric measurements, lasting only up to ∼750 h before replacement is required. Halogen lamps will soon be phased out in favor of new longer-lasting light-emitting diodes (LED) in commercial devices.</div></div><div><h3>Methods</h3><div>We compared the analytical performance of the new Cobas® c 703 analytical unit (LED; Cobas Pro integrated solutions) versus the Cobas c 701/c 702 modules (halogen light source; Cobas 8000 modular analyzer series; all Roche Diagnostics International Ltd). At two evaluation sites, precision of the c 703 analytical unit was evaluated and method comparison experiments comparing the c 703 analytical unit versus c 701/c 702 modules were performed.</div></div><div><h3>Results</h3><div>Results from 34 selected applications showed robust precision of the c 703 analytical unit (repeatability, intermediate precision, and reproducibility: 80/138 coefficients of variation &lt;1 %, 60/138 &lt; 1 %, and 48/69 &lt; 2 %, respectively). Method comparison experiments with 31 applications showed good comparability between c 703 and c 701/c 702 using LED versus halogen light sources, respectively (median slope = 1.00; median bias = 0.2 %; median Pearson's correlation coefficient = 0.997).</div></div><div><h3>Conclusions</h3><div>In addition to the newly introduced LED light source having a longer lifetime (10,000 h; data not shown), the c 703 analytical unit demonstrated equivalent analytical performance to that of the c 701/c 702 modules using halogen lamps as the light source as part of high-throughput clinical chemistry analyzers.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"46 ","pages":"Article e00482"},"PeriodicalIF":1.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of blood centrifugation on the parameters of thrombin generation assay revisited to look for possible revision of the current guidance","authors":"Erica Scalambrino ,&nbsp;Marigrazia Clerici ,&nbsp;Flora Peyvandi ,&nbsp;Armando Tripodi","doi":"10.1016/j.plabm.2025.e00478","DOIUrl":"10.1016/j.plabm.2025.e00478","url":null,"abstract":"<div><div>Blood centrifugation affects thrombin generation assays (TGA). Current guidance recommends double-centrifugation, which is uncommon in clinical laboratories.</div><div>We evaluated the impact of 4 centrifugation speeds on TGA performed with low-triggers (1pM tissue-factor/1.0 μM phospholipids) or high-triggers (5pM tissue-factor/5.0 μM phospholipids). TGA parameters were evaluated in the presence/absence of thrombomodulin.</div><div>We included 20 healthy subjects. Centrifugation speeds were: (i)Double-centrifugation: blood at 2,500g(15min) and plasma at 2500(15min) (reference method). (ii)Single-centrifugation at 3,000g(20min). (iii)Single-centrifugation of blood at 3,000g(20min), plasma freezing, then centrifugation of thawed plasma at 10,000g(5min). (iv)Single-centrifugation at 1,700g(10min). Results were also expressed as percentage difference relative to reference centrifugation.</div><div>Lag-time was affected when centrifugation speed was relatively slow (1,700g), regardless of low- or high-triggers, presence or absence of thrombomodulin, whereas it was scarcely affected by centrifugation at 3,000g. Peak-thrombin was marginally affected at relatively low-speed (1,700g). ETP was marginally affected at relatively low-speed (1,700g), except when TGA was performed in the presence of thrombomodulin. Peak-thrombin and ETP were not or were poorly affected by centrifugation at 3,000g or 10,000g after thawing, respectively.</div><div>In conclusion, slow-centrifugation (1,700g) had a considerable impact on lag-time. This centrifugation speed represents common practice in clinical laboratories and should not be used for TGA, unless controls samples centrifuged at the same speed are used for comparison. Single-centrifugation at 3,000g may be a suitable alternative, which would allow TGA testing without the complex and time-consuming double-centrifugation as recommended by current guidance. We propose that current guidance on plasma preparation for TGA be switched from double-to a more intense single-centrifugation.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"45 ","pages":"Article e00478"},"PeriodicalIF":1.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance verification of the new UF-1500 urine particle analyser: a new opportunity for small and medium laboratories","authors":"Giulia Previtali,&nbsp;Michela Seghezzi,&nbsp;Roberto Marozzi,&nbsp;Monica Fortino,&nbsp;Gianluca Agnolet,&nbsp;Mauro Barretta,&nbsp;Claudia Bizzoni,&nbsp;Valeria Bolla,&nbsp;Greta Bolzoni,&nbsp;Alessia Cesani,&nbsp;Matteo Diambrini,&nbsp;Sara Apassiti Esposito,&nbsp;Giorgia Giuliani,&nbsp;Alina Picciau,&nbsp;Maria Grazia Alessio","doi":"10.1016/j.plabm.2025.e00481","DOIUrl":"10.1016/j.plabm.2025.e00481","url":null,"abstract":"<div><h3>Background</h3><div>The UF-1500 is the new fully automated urine particle analyser by Sysmex specifically tailored for small and medium laboratories. This study aim to validate its analytical and diagnostic performance.</div></div><div><h3>Methods</h3><div>754 first morning mid-stream urines were analysed on UF-1500; 550 samples were used for the UF-5000 comparison and 204 were used for the correlation with manual count on Fuchs-Rosenthal chamber. Carry-over, linearity and imprecision of the UF-1500 were also assessed.</div></div><div><h3>Results</h3><div>Correlation with the UF-5000 was excellent, with <em>r</em> coefficient range between 0.88 and 1.00. Correlation with Fuchs-Rosenthal chamber was very good for all the parameters; <em>r coefficient</em> ranged between 0.67 and 0.94. Linearity regression coefficient of determination (R²) was excellent for almost all the parameters. No carry-over was observed. The within-run imprecision range between 2.93 % for RBC and 35.63 % for WBC. The between-run imprecision ranged between 2.1 % for RBC and 23.9 % for CAST, using low and high positive quality controls, respectively.</div></div><div><h3>Conclusion</h3><div>The analytical and diagnostic performance is satisfactory for almost all the parameters, when compared with the UF-5000; the correlation with the reference method is equally good.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"46 ","pages":"Article e00481"},"PeriodicalIF":1.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct identification of Mycobacterium species from liquid medium (MGIT) using FastPrep-2 bead beating system and MALDI-TOF mass spectrometry technology: a comparison with solid media and PCR-based method","authors":"Ali M. Bazzi ,&nbsp;Abdulaziz A. Sunki ,&nbsp;Mohab J. Hamdi ,&nbsp;Abdullah O. Khaldi ,&nbsp;Jaffar A. Al-Tawfiq","doi":"10.1016/j.plabm.2025.e00479","DOIUrl":"10.1016/j.plabm.2025.e00479","url":null,"abstract":"<div><h3>Background</h3><div>Mycobacterial infections present significant global health challenges. Traditional diagnostic methods are inadequate for the identification of Mycobacterium species, highlighting the need for more efficient techniques. Matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) technology offers potential advantages in rapid and accurate pathogen identification.</div></div><div><h3>Objective</h3><div>This study evaluates the accuracy and precision of the MALDI-TOF using the FastPrep-2 bead beating system and VITEK MS version 3.2 for identifying Mycobacterium species directly from Mycobacteria Growth Indicator Tube (MGIT), liquid medium, compared to MALDI-TOF (VITEK MS) based on the traditional solid medium (Lowenstein–Jensen). We also compared the result of the MALDI-TOF from MGIT to <em>M tuberculosis</em> results by PCR-based method.</div></div><div><h3>Methods</h3><div>The study included 16 <em>mycobacterium tuberculosis</em> (MTB) and 37 <em>nontuberculous mycobacteria</em> (NTM). Isolates were grown in LJ solid medium and MGIT liquid medium, and lysed using the FastPrep-2 bead beating system. Identification was performed using VITEK MS version 3.2 from liquid.</div></div><div><h3>Results</h3><div>NTM comprised 70 % (37/53) of the total isolates evaluated. <strong>Of these, 92 % (34/37) were successfully identified using VITEK MS from MGIT liquid medium.</strong> Overall, the method achieved 88.6 % accuracy for identifying <em>Mycobacterium</em> species from liquid medium, compared to 96.2 % from solid medium. The agreement between both methods was moderate (Kappa = 0.470, p &lt; 0.001). MTB isolates were identified with 100 % accuracy, and the approach demonstrated excellent reproducibility with 100 % intra-assay and inter-day consistency.</div></div><div><h3>Conclusion</h3><div>Using VITEK MS version 3.2 to directly identify MTB and NTM from MGIT liquid medium provides a rapid, cost-effective, and reliable method for identifying Mycobacterium species. Further optimization and database expansion are recommended to enhance accuracy for rare and less common mycobacterial species.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"46 ","pages":"Article e00479"},"PeriodicalIF":1.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical performance evaluation of intelligent quality management of blood gas analyzer","authors":"Hongting Tang ,&nbsp;Yawen Xiao ,&nbsp;Hong Luo,&nbsp;Jian Jiang,&nbsp;Hanqing Xu,&nbsp;Jun Yang,&nbsp;Lihua Yang,&nbsp;Xiang Yang","doi":"10.1016/j.plabm.2025.e00480","DOIUrl":"10.1016/j.plabm.2025.e00480","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to compare the application effectiveness and quality control (QC) performance of intelligent quality management for blood gas analysis (BGA) with those of traditional quality management.</div></div><div><h3>Methods</h3><div>We implemented intelligent quality management by employing the GEM Premier 5000 equipped with Intelligent Quality Management 2 (iQM 2). By collecting external quality assessment (EQA) and internal quality control (IQC) data, we compared the clinical application outcomes and quality control (QC) performance between the intelligent management and traditional management approaches.</div></div><div><h3>Results</h3><div>The average bias of EQA for pH, partial carbon dioxide pressure (pCO₂), partial oxygen pressure (pO₂), sodium (Na⁺) and calcium (Ca²⁺) decreased compared to pre-management levels; except for pO₂, the average coefficient of variation (CV%) of intelligent QC was lower. The average estimated total error (TE) in the intelligent QC met the specified acceptance criterion. According to the average sigma and the goal index ratio (QGI), both QC modes have issues with accuracy and precision; the probabilities of false rejection (Pfr) of traditional QC and intelligent QC are almost the same; except for pO₂ and Na⁺, the probability of error detection (Ped) of intelligent QC is greater, whereas the average detection time (ADT) of traditional QC is greater. In addition, intelligent QC identified errors in approximately 1.46 % of the samples.</div></div><div><h3>Conclusions</h3><div>The precision and accuracy of the BGA improved significantly compared to those before management, indicating significant advantages of intelligent quality management in quality management applications.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"45 ","pages":"Article e00480"},"PeriodicalIF":1.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing mutation-clinical correlations and treatment outcomes in Vietnamese non-small cell lung cancer patients","authors":"Hoang-Bac Nguyen ,&nbsp;Bang-Suong Nguyen-Thi ,&nbsp;Huu-Huy Nguyen ,&nbsp;Minh-Khoi Le ,&nbsp;Quoc-Trung Lam ,&nbsp;Tuan-Anh Nguyen","doi":"10.1016/j.plabm.2025.e00477","DOIUrl":"10.1016/j.plabm.2025.e00477","url":null,"abstract":"<div><h3>Introduction</h3><div>This study examines the genetic and clinical profiles of Vietnamese patients with non-small cell lung cancer (NSCLC), focusing on mutations in seven driver genes: <em>EGFR</em>, <em>KRAS</em>, <em>NRAS</em>, <em>BRAF</em>, <em>ALK</em>, <em>ROS1</em>, and <em>PIK3CA</em>. The goal is to identify mutation patterns and their correlations with clinical factors, thereby informing personalized treatment strategies.</div></div><div><h3>Materials and methods</h3><div>A cross-sectional study of 299 NSCLC patients at the University Medical Center, Ho Chi Minh City (2019–2022) recorded demographics, smoking history, and tumor stage. Pre-treatment samples were analyzed via massively parallel sequencing, and survival analysis assessed the impact of <em>EGFR/KRAS</em> mutations on survival and TKI response.</div></div><div><h3>Results</h3><div>Most patients (88.6 %) were diagnosed at stage IV. <em>EGFR</em> mutations were found in 43.5 % of cases, predominantly in female non-smokers, while <em>KRAS</em> mutations (15.4 %) were more common in male smokers. <em>EGFR</em> exon 19 deletions (46.3 %) and L858R (39.0 %) were the most frequent, with <em>KRAS</em> G12C (29.8 %) as the dominant variant. <em>EGFR</em>-mutant patients treated with TKIs had significantly longer survival (p = 0.027); however, no survival difference was observed between the <em>EGFR</em>- and <em>KRAS</em>-mutated groups. Co-mutations (3.7 %) were rare but may indicate resistance. Logistic regression confirmed <em>EGFR</em> mutations' association with female non-smokers and <em>KRAS</em> mutations with male smokers.</div></div><div><h3>Conclusions</h3><div>Genetic profiling in Vietnamese NSCLC patients reveals a high prevalence of actionable driver mutations, supporting the integration of routine molecular testing into NSCLC management. <em>EGFR</em>-mutated patients derive significant benefits from TKI therapy, underscoring the importance of personalized treatment strategies. Further research is needed to investigate resistance mechanisms and refine targeted therapeutic approaches.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"45 ","pages":"Article e00477"},"PeriodicalIF":1.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SII as a predictor of mortality in patients with non-ST-segment elevation myocardial infarction and diabetes mellitus","authors":"Cuiyuan Huang ,&nbsp;Jiajuan Yang ,&nbsp;Wenqiang Li ,&nbsp;Li Liu ,&nbsp;Wei Wang ,&nbsp;Haiyan Hu ,&nbsp;Jing Zhang ,&nbsp;Jian Yang","doi":"10.1016/j.plabm.2025.e00476","DOIUrl":"10.1016/j.plabm.2025.e00476","url":null,"abstract":"<div><h3>Background</h3><div>Systemic immune inflammation index (SII) is an innovative marker reflecting immune and inflammatory responses.</div></div><div><h3>Objectives</h3><div>To explore the predictive value of SII on the risk of death in patients with NSTEMI combined with T2DM.</div></div><div><h3>Methods</h3><div>An analysis of 448 patients with NSTEMI and T2DM admitted to our institution between December 2017 and May 2022 was conducted in this retrospective study. SII values were used to divide patients into high and low SII groups and investigate their impact on mortality.</div></div><div><h3>Results</h3><div>According to the analysis results, elevated SII levels are significantly linked to a poor prognosis in patients with NSTEMI and T2DM. Over an average follow-up period of 22.75 months, 106 (23.7 %) all-cause deaths were recorded. The optimal threshold for predicting death was found to be an SII value of 1384.596 × 10⁹/L through ROC curve analysis. Kaplan-Meier analysis indicated that the survival rates were higher in the low SII group compared to the high SII group (<em>P</em> &lt; 0.001). Elevated SII levels were independently linked to increased mortality in patients with NSTEMI and T2DM, according to univariate (HR:3.19, 95 % Cl: 2.18–4.68) and multivariate COX (HR: 2.72, 95 % Cl: 1.81–4.09) regression analyses.</div></div><div><h3>Conclusion</h3><div>High SII values were strongly associated with mortality in patients with NSTEMI and T2DM. SII serves as a valuable prognostic tool, enhancing the management and prognosis of patients with concurrent NSTEMI and T2DM.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"45 ","pages":"Article e00476"},"PeriodicalIF":1.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breath fingerprint of colorectal cancer patients by gas chromatography-mass spectrometry analysis preparatory to e-nose analyses","authors":"Stefano Dugheri ,&nbsp;Ilaria Rapi ,&nbsp;Giovanni Cappelli ,&nbsp;Niccolò Fanfani ,&nbsp;Donato Squillaci ,&nbsp;Simone De Sio ,&nbsp;Beatrice Mallardi ,&nbsp;Paola Mantellini ,&nbsp;Fabio Staderini ,&nbsp;Veronica Traversini ,&nbsp;Antonio Baldassarre ,&nbsp;Fabio Cianchi ,&nbsp;Nicola Mucci","doi":"10.1016/j.plabm.2025.e00475","DOIUrl":"10.1016/j.plabm.2025.e00475","url":null,"abstract":"<div><div>Colorectal cancer (CRC), according to the most recent data provided by GLOBOCAN, ranks fourth worldwide in incidence and third in mortality among all cancers. Current estimates project a global increase in colorectal cancer incidence of 60.5 % and mortality of 76.9 % between 2022 and 2045. The low sensitivity and adherence, coupled with the high costs associated with current diagnostic methods for CRC, underscore the need to explore innovative procedures for the early detection of tissue abnormalities. Existing research suggests that patients affected by this condition exhibit distinctive alterations in volatile organic compounds (VOCs) ratios in their exhaled breath.</div><div>This study presents a characterization of exhaled breath using Gas Chromatography-Mass Spectrometry (GC-MS) in patients with varying stages of the disease, as determined by conventional medical and clinical analyses. An electronic nose was utilized to develop a method aimed at rapidly analyzing a subject's exhaled breath to identify the group of belonging (healthy, affected). The aim of the study was to develop a rapid, cost-effective, and non-invasive early diagnostic system employing an electronic nose. Statistical analysis identified 12 compounds with the potential to distinguish between healthy and affected individuals and were selected for testing the application potential of the Cyranose 320 electronic nose. The ability of the method to identify the 40 subjects analyzed as Healthy Controls (HC) or CRC in terms of sensitivity and specificity (0.8 and 0.85, respectively) demonstrates the feasibility of using this method for rapid, low-cost, and non-invasive disease recognition.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"45 ","pages":"Article e00475"},"PeriodicalIF":1.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1,25-dihydroxyvitamin D in the elderly population: Comparison of liquid chromatography tandem mass spectrometry and CLIA immunoassay (LIAISON®XL) methods","authors":"Dorota Leszczyńska ,&nbsp;Alicja Szatko ,&nbsp;Magdalena Ostrowska ,&nbsp;Magdalena Zgliczyńska ,&nbsp;Konrad Kowalski ,&nbsp;Wojciech Zgliczyński ,&nbsp;Piotr Glinicki","doi":"10.1016/j.plabm.2025.e00474","DOIUrl":"10.1016/j.plabm.2025.e00474","url":null,"abstract":"<div><h3>Background</h3><div>1α,25-dihydroxyvitamin D is the biologically active form of vitamin D₃ (cholecalciferol) and vitamin D₂ (ergocalciferol). The determination of 1,25(OH)₂D is clinically relevant in the diagnostics of vitamin D metabolism disorders, PTH-independent hypercalceamia and hypophosphatemic syndromes. The quantitative assessment of 1,25(OH)₂D may be a challenge since it circulates in picomolar concentrations in the blood.</div></div><div><h3>Aims of the study</h3><div>Comparison of two methods: chemiluminescent immunoassay (CLIA, LIAISON®XL) and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) for determinations of 1,25(OH)₂D concentrations in elderly populations. The secondary aim was to assess correlation between 1,25(OH)₂D for CLIA_LIAISON®XL and LC-MS/MS methods and selected factors (vitamin D metabolites, calcium, albumin, PTH, creatinine concentrations in serum and age).</div></div><div><h3>Materials and methods</h3><div>The study was conducted on 54 patients aged from 60 to 96 at the Bielański Hospital in Warsaw, Poland. The determination of 1,25(OH)₂D using CLIA_LIAISON®XL and LC-MS/MS methods was performed.</div></div><div><h3>Results</h3><div>Both methods (CLIA_LIAISON®XL immunoassay and LC-MS/MS technique) were strongly positively correlated (r = 0.86; p &lt; 0.001). In the LC-MS/MS technique, concentration of 1,25(OH)₂D was significantly higher compared to the CLIA_LIAISON®XL immunoassay. The regression equation revealed method interchangeability. Concentration of 1,25(OH)₂D was significantly correlated with various basic biochemical parameters (albumin and calcium levels) for both methods.</div></div><div><h3>Conclusions</h3><div>In our study, measurement of 1,25(OH)₂D using CLIA_LIAISON®XL was not inferior to LC-MS/MS measurement. The assessment of 1,25(OH)₂D using CLIA_LIAISON®XL, characterized by short turnaround time, low costs and high accuracy, may be an optimal choice for elderly patients who often require prompt diagnosis and treatment.</div></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"45 ","pages":"Article e00474"},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}