Influence of plasma collection tubes on N-glycome in human blood samples

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY
Zejian Zhang , Xiangyi Cui , Nan Zhou , Lisi Zhu , Yuxiang Zhi , Shuyang Zhang
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引用次数: 0

Abstract

Background and aims

Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.

Materials and methods

We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.

Results

Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits.

Conclusion

Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.

Abstract Image

血浆采集管对人体血液样本中 N-糖蛋白的影响
背景和目的定量分析血浆N-甘氨酸是一种很有前景的确定疾病生物标志物的方法。本研究旨在调查使用不同抗凝剂的采血管对血浆 N-lycome的影响。材料和方法我们使用一种稳健的质谱方法对两组健康志愿者(组群 1,n = 16;组群 2,n = 53)的血浆 N-lycome进行了分析。结果主要成分分析表明,根据采血管的不同,血样的聚类也不同。配对比较显示,在 82 个量化的 N-糖特征中,EDTA 和肝素血浆在 55 个特征上存在显著差异;在 82 个特征中,EDTA 和柠檬酸血浆在 62 个特征上存在显著差异。这些差异涵盖了各种 N-聚糖特征,包括聚糖类型、硅烷基化、半乳糖基化、岩藻糖基化和双链化。与 EDTA 血浆相比,柠檬酸盐和肝素血浆中 N 聚糖的变化趋势基本一致。在相关性分析(EDTA 与肝素;EDTA 与枸橼酸盐)中,大多数 N-糖特征的皮尔逊相关系数始终高于 0.7。结论样品基质的变化会影响血浆 N-糖蛋白的测量,在糖化学项目中比较来自不同血浆收集管的样品时必须谨慎。
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来源期刊
Practical Laboratory Medicine
Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology
CiteScore
3.50
自引率
0.00%
发文量
40
审稿时长
7 weeks
期刊介绍: Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.
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