Practical Laboratory Medicine最新文献

{"title":"Comparative and stability study of glucose concentrations measured in both sodium fluoride and serum separator tubes","authors":"Mustapha Dibbasey,&nbsp;Solomon Umukoro,&nbsp;Abdoulie Bojang","doi":"10.1016/j.plabm.2024.e00360","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00360","url":null,"abstract":"<div><h3>Introduction</h3><p>Sodium fluoride/potassium oxalate (NaF/KOx) tubes has been regarded as the gold-standard tubes for glucose analysis. Even though their ineffectiveness in immediately inhibiting glycolysis has been reported in several studies especially in the first 1–4h, they are still used in our clinical biochemistry laboratory for glucose measurement. However, in its absence, only serum separator tubes are employed for glucose measurement. We aim to determine whether serum separator tubes (SSTs) can replace NaF/KOx tubes for laboratory measurement of blood glucose and to assess the stability of glucose concentrations for 3 days period.</p></div><div><h3>Methods and findings</h3><p>NaF/KOx tube type was the reference method while SSTs type was the candidate method for glucose measurement. A total of 50 paired samples collected separately in NaF/KOx tubes and SSTs from healthy adult participants in the Gambia Adults Reference Intervals Study (GARIS) project were used as the project sample size. Following blood collection and separation, the glucose concentration was measured within 2 h, and at 24h, 42h and 72h time-points. Our data analysis showed no significant difference in the mean glucose concentrations between the reference tube and candidate tube types (Mean difference = 0.06 mmol/L; P = 0.38) recorded in the different timepoints. Using growth trajectory and mixed effects model, the study data further showed no significant change in the glucose concentrations (p = 0.25) for three days period.</p></div><div><h3>Conclusions</h3><p>The study confirms that SSTs can produce similar glucose results when employed in the absence of NaF/KOx tubes. Besides, the glucose concentrations were stable in both tubes for three days when the samples were separated within 2 h and refrigerated in 2–8°C.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00360"},"PeriodicalIF":1.9,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000064/pdfft?md5=853bc903207429646d72e91070a938b5&pid=1-s2.0-S2352551724000064-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139653560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a simple, fast and sensitive liquid chromatography-tandem mass spectrometry method to establish reference intervals for 24-h urinary free normetanephrine, metanephrine and methoxytyramine","authors":"Yan Song ,&nbsp;Runhao Xu ,&nbsp;Dan Liu ,&nbsp;Jie Zhang","doi":"10.1016/j.plabm.2024.e00358","DOIUrl":"10.1016/j.plabm.2024.e00358","url":null,"abstract":"<div><h3>Objective</h3><p>To develop and validate a rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect urinary free metanephrines and methoxytyramine, establishing reference intervals.</p></div><div><h3>Methods</h3><p>Urine samples were diluted with isotope internal standard solution, then analyzed directly using tandem mass spectrometry with multiple reaction monitoring measurement and electrospray ionization source in positive ion mode. Analytical parameters including linearity, lower limit of quantitation, imprecision and accuracy of the method were evaluated. The reference intervals for urinary catecholamine metabolites were established by analyzing 24-h urine samples collected from 81 apparently healthy volunteers.</p></div><div><h3>Results</h3><p>The analytical times for MN, NMN, and 3-MT were at 2.79, 2.80, and 2.74 min, respectively. The method displayed excellent linearity (r &gt; 0.99) in the range of 1-1000 ng/mL, with lower limits of quantification (LLOQ) at 0.50 ng/mL for MN and NMN, and 0.25 ng/mL for 3-MT. The method's intra-day and inter-day imprecisions were less than 8 %. The method recovery ranged from 96.8% to 105.8 % for MN, 89.7%–106.4 % for NMN, and 93.5%–106.2 % for 3-MT. No carry-over was observed during the analysis of all analytes. The LC-MS/MS method was used to establish reference intervals in 24-h urine samples from 81 apparently healthy volunteers. There was no association of sex with urinary free metabolites.</p></div><div><h3>Conclusion</h3><p>This study established a novel, fast and sensitive LC-MS/MS method for determining urinary free catecholamine metabolites, which could facilitate screening and diagnosis for catecholamine-related tumors more conveniently and quickly.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00358"},"PeriodicalIF":1.9,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000040/pdfft?md5=be00b0e547aca2b06fab6571e7593cd0&pid=1-s2.0-S2352551724000040-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139633875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of plasma aldosterone measured by chemiluminescence immunoassay and liquid chromatography-tandem mass spectrometry in screening test for primary aldosteronism","authors":"Wenzhan Chen ,&nbsp;Fenghua Lai ,&nbsp;Xiaoyu Huang,&nbsp;Shuang Yu,&nbsp;Nan Chen,&nbsp;Changliu Xu,&nbsp;Chenxue Wang,&nbsp;Shuhui Liang,&nbsp;Yanbing Li,&nbsp;Haipeng Xiao,&nbsp;Xiaopei Cao","doi":"10.1016/j.plabm.2024.e00361","DOIUrl":"10.1016/j.plabm.2024.e00361","url":null,"abstract":"<div><h3>Background</h3><p>Whether chemiluminescence immunoassay (CLIA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for plasma aldosterone concentration (PAC) measurement can be used interchangeably in primary aldosteronism (PA) screening is still controversial. The purpose of this study was to compare CLIA to LC-MS/MS for PAC measurement in PA screening.</p></div><div><h3>Methods</h3><p>All participants underwent aldosterone-to-renin ratio (ARR) testing. PA was diagnosed by captopril challenge test or saline infusion test. PAC in screening test was measured with CLIA and LC-MS/MS. Plasma direct renin concentration in screening and confirmatory test was measured with CLIA. The concordance between CLIA and LC-MS/MS for PAC measurement in PA screening was analyzed.</p></div><div><h3>Results</h3><p>Twenty-one healthy volunteers, 61 patients with essential hypertension (EH) and 43 PA patients were enrolled. Median PAC by CLIA was 84.7 % higher than that by LC-MS/MS in screening test (<em>P</em> &lt; 0.001). A positive correlation of PAC was observed between the two assays (Pearson <em>r</em> coefficient 0.770, <em>P</em> &lt; 0.001). When ARR was used in differentiating PA from EH, there was no difference in the area under the receiver operating characteristic curve between CLIA and LC-MS/MS for PAC measurement (0.968 <em>vs</em> 0.950, <em>P</em> = 0.249).</p></div><div><h3>Conclusion</h3><p>CLIA and LC-MS/MS for PAC measurement exhibited high and comparable efficacy in PA screening. CLIA is a reliable and feasible alternative in PA screening test.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00361"},"PeriodicalIF":1.9,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000076/pdfft?md5=8e7b6dc11f5e503e0cace75b3ec0907e&pid=1-s2.0-S2352551724000076-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139634654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological predictors of preeclampsia among pregnant women attending ante-natal clinic at Arba Minch General Hospital, South Ethiopia: A comparative cross-sectional study","authors":"Woldeteklehaymanot Kassahun ,&nbsp;Aschalew Kidanewold ,&nbsp;Getahun Koira ,&nbsp;Gelila Biresaw ,&nbsp;Mulu Shiferaw","doi":"10.1016/j.plabm.2024.e00362","DOIUrl":"10.1016/j.plabm.2024.e00362","url":null,"abstract":"<div><h3>Background</h3><p>Preeclampsia is a kind of pregnancy-related hypertension that affects 5.47 % of pregnancies in Ethiopia and 18.25 % of pregnant women who visit Arba Minch public health facilities for antenatal care. This study sought to identify hematological preeclampsia markers in pregnant women who received prenatal care at Arba Minch General Hospital.</p></div><div><h3>Methodology</h3><p>An institution-based comparative cross-sectional study was done from July 22 to October 30, 2021 at Arba Minch General Hospital. A total of 136 pregnant women were included in the study (46 with preeclampsia and 90 without preeclampsia). Epidata version 4.4. was used to enter data, and SPSS version 25.0 and Stata version17 were used for analysis. An independent sample <em>t</em>-test was used to examine the hematological parameter differences between study groups. Potential hematological markers were determined using receiver operating characteristic (ROC) analysis of the area under the curve (AUC). Statistical significance was defined if P value less than 0.05.</p></div><div><h3>Results</h3><p>A total of 136 pregnant women were studied. The complete blood count analysis showed that there were means differences in Red Cell Distribution (RDW) (p &lt; 0.036), neutrophil-to-lymphocyte ratio (NLR) (p &lt; 0.016) and relative lymphocyte count (Lymp%) (p &lt; 0.047). The ROC analysis of the AUC for RDW, NLR and Lymp% resulted in 0.607, 0.609, 0.600 respectively.</p></div><div><h3>Conclusion</h3><p>RDW, NLR and Lymphocyte count could be potential candidate tools for the diagnosis and screening of preeclampsia. However, the robustness of the markers should be tested with prospective studies assessing changes present in each trimester.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00362"},"PeriodicalIF":1.9,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000088/pdfft?md5=7b3c4c9dd005154474ea25ec1eb45193&pid=1-s2.0-S2352551724000088-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139537197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two methods for dimethylarginines quantification","authors":"Vendula Sudová ,&nbsp;Pavel Prokop ,&nbsp;Ladislav Trefil ,&nbsp;Jaroslav Racek ,&nbsp;Daniel Rajdl","doi":"10.1016/j.plabm.2024.e00359","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00359","url":null,"abstract":"<div><h3>Objectives</h3><p>Both dimethylarginines are widely bound to chronic kidney disease (CKD). This study was focused to validate published LC-MS/MS method and compared the measured data with an immunoassay.</p></div><div><h3>Design and methods</h3><p>The analysis was performed on a Dionex UltiMate 3000 UHPLC-Standard (Thermo Fisher Scientific, Waltham, Massachusetts, USA) with an amaZon SL ion trap (Bruker, Billerica, Massachusetts, USA). Comparison was evaluated by using Passing Bablok regression and Bland Altman plot. Healthy volunteers (n = 40) were used for validation and as control group to patients group (n = 40) with different stages of CKD.</p></div><div><h3>Results</h3><p>The results in healthy controls determined by the LC-MS/MS (ELISA) method were 0.52 ± 0.0892 with 95 % CI: 0.49–0.55 (0.61 ± 0.1213 with 95 % CI: 0.57–0.64) μmol/L for AD MA and 0.56 ± 0.0810 with 95 % CI: 0.53–0.58 (0.62 ± 0.0752 with 95 % CI: 0.57–0.65) μmol/L for SDMA. In the same way, the patient group values determined by the LC-MS/MS (ELISA) method were 0.82 ± 0.1604 with 95 % CI: 0.75–0.88 (1.06 ± 0.3002 with 95 % CI: 0.94–1.19) μmol/L and 2.14 ± 0.8778 with 95 % CI: 1.47–2.58 (1.65 ± 0.5160 with 95 % CI: 1.40–1.98) μmol/L for ADMA and SDMA, respectively. The correlation between the methods, expressed as the Spearman correlation coefficient (R), was 0.858 (0.8059) for ADMA (p &lt; 0.0001) and 0.895 (0.9607) for SDMA (p &lt; 0.0001).</p></div><div><h3>Conclusions</h3><p>ADMA levels determined by the immunoassay were almost 30 % overestimated, in contrast to SDMA levels, which were 3 % underestimated. According to our findings, a better correlation could be obtained by simple sample dilution.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00359"},"PeriodicalIF":1.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000052/pdfft?md5=4abd9e1a9454feaf56a37f9a9380d981&pid=1-s2.0-S2352551724000052-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures","authors":"Tze Ping Loh ,&nbsp;Brian R. Cooke ,&nbsp;Thi Chi Mai Tran ,&nbsp;Corey Markus ,&nbsp;Rosita Zakaria ,&nbsp;Chung Shun Ho ,&nbsp;Elvar Theodorsson ,&nbsp;Ronda F. Greaves ,&nbsp;IFCC Working Group on Method Evaluation Protocols (WG-MEP)","doi":"10.1016/j.plabm.2024.e00355","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00355","url":null,"abstract":"<div><p>Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers, and editors in deciding the quality of a submitted manuscript.</p><p>The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00355"},"PeriodicalIF":1.9,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000015/pdfft?md5=f1ca2d253fbe730a00389903c37a7dda&pid=1-s2.0-S2352551724000015-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139505420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical assessment and validation of the ProteinSimple ELLA serum B-cell maturation antigen assay","authors":"Daniel Conrad Kirchhoff,&nbsp;Wei Zhang,&nbsp;Athanasia Chandras,&nbsp;Damodara Rao Mendu","doi":"10.1016/j.plabm.2023.e00354","DOIUrl":"https://doi.org/10.1016/j.plabm.2023.e00354","url":null,"abstract":"<div><h3>Objectives</h3><p>Soluble B-Cell Maturation Antigen (sBCMA) is a degradation product of plasma cell-bound BCMA found in serum. Serum sBCMA concentrations correlate with bone marrow plasma cellularity, making it an attractive biomarker for monitoring plasma cell disorders, such as multiple myeloma. Here we evaluated the automated BCMA immunoassay for the ProteinSimple ELLA, for the analysis of sBCMA.</p></div><div><h3>Design &amp; methods</h3><p>Inter and intra-run precision was assessed through replicate sBCMA measurements at 3 different concentration levels. Linearity was determined through serial dilution of a high sBMCA patient sample. Accuracy was assessed through split specimen analysis on two separate lots of reagents. Stability was assessed at 3 temperature levels over 14 days. Cross-reactivity was assessed on BCMA targeting and non-targeting chemotherapeutics. A reference range was established through the analysis of 146 healthy donor samples. The effect of endogenous interferents was assessed through spiking and recovery studies.</p></div><div><h3>Results</h3><p>Inter and intra-run precision studies afforded CVs of &lt;10% at all three concentration levels. Analytical measurement range was confirmed from 0.1 to 7 ng/mL. Accuracy studies afforded a slope of 0.976, intercept of 1.22, R² of 0.996. Assayed sBCMA values were unaffected by endogenous interferents and non-BMCA targeting antibodies. BCMA targeting therapeutics negatively affected assayed sBCMA concentrations. The reference range was established at 19–58 ng/mL sBCMA is analytically stable.</p></div><div><h3>Conclusions</h3><p>The ProteinSimple ELLA sBCMA assay shows acceptable performance for the clinical assessment of sBCMA. The assay was highly affected by BCMA targeting therapeutics, thereby patients undergoing this therapy should not have their sBCMA levels assessed by this method.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"38 ","pages":"Article e00354"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551723000483/pdfft?md5=9b9411a7476dae998d012d5ec1e6ae83&pid=1-s2.0-S2352551723000483-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139433508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a fluorescence immunochromatography method for quantitative measurement of matrix metalloproteinase-9","authors":"Jingyan Huang ,&nbsp;Cuicui Chen ,&nbsp;Huankun Liang ,&nbsp;Wenqi Dong ,&nbsp;Laiqing Li ,&nbsp;Hongyan Ma","doi":"10.1016/j.plabm.2024.e00356","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00356","url":null,"abstract":"<div><h3>Objective</h3><p>Abnormal serum matrix metalloproteinase-9 (MMP-9) levels are closely related to the occurrence and development of many diseases. This study aimed to establish a fluorescence immunochromatography (FIC) method using the lanthanide fluorescent element europium(III) (Eu³⁺) for the quantitative measurement of MMP-9 in serum.</p></div><div><h3>Design &amp; Methods</h3><p>The FIC method for quantifying MMP-9 was optimized and established, and the FIC test strips (FICTS) were assembled and subsequently evaluated for sensitivity, specificity and precision. Furthermore, the reference interval and clinical sensitivity/specificity were estimated using clinical healthy/positive serum samples, and a commercial ELISA was used for comparison.</p></div><div><h3>Results</h3><p>We successfully established an FIC method and prepared FICTS. The analytical sensitivity of the FICTS was 0.92 ng/mL, with a linearity range of 0–1000 ng/mL. The cross-reactivity of the 7 common serum interferents was less than 1.56%. All recoveries of the intra-array and inter-array samples ranged from 102.50% to 110.99%, and all <em>CVs</em> were less than 5%. The reference interval of the FICTS was &gt;161.15 ng/mL. The clinical sensitivity was 96.00%, and the specificity was 97.5%. The results of 270 clinical serum samples were highly coincident with the clinical diagnostic results. Pearson correlation analysis and Bland‒Altman plots indicated that the FICTS and commercial ELISA results were consistent with the quantitative MMP-9 concentration.</p></div><div><h3>Conclusions</h3><p>The designed FIC method and test strips may be suitable for point-of-care quantitative measurement of MMP-9, which provides a new method for screening for atherosclerosis, xerophthalmia, etc.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"38 ","pages":"Article e00356"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000027/pdfft?md5=283e5035d274edefb4061b80dbdd444a&pid=1-s2.0-S2352551724000027-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139487291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of results and age-related changes in establishing reference intervals for CEA, AFP, CA125, and CA199 using four indirect methods","authors":"Juping Chen ,&nbsp;Lina Fan ,&nbsp;Zheng Yang ,&nbsp;Dagan Yang","doi":"10.1016/j.plabm.2023.e00353","DOIUrl":"https://doi.org/10.1016/j.plabm.2023.e00353","url":null,"abstract":"","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"38 ","pages":"Article e00353"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551723000471/pdfft?md5=42ad9ed6fec02768065ea6494843fd0a&pid=1-s2.0-S2352551723000471-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139107801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative real-time PCR and magnetic separation strategy for specific detection of group B streptococcus in perinatal Women's urine","authors":"Xu Tang ,&nbsp;Lin-Ping Fan ,&nbsp;Yang Liu","doi":"10.1016/j.plabm.2023.e00348","DOIUrl":"https://doi.org/10.1016/j.plabm.2023.e00348","url":null,"abstract":"<div><h3>Introduction</h3><p>Group B streptococcus(GBS)often causes adverse outcomes such as urinary system infection, intrauterine infection, premature birth, and stillbirth in perinatal women. Perinatal screening of GBS is conducive to guiding clinical scientific intervention and improving delivery outcomes.This study quantitative real-time PCR (RT-qPCR) combined with magnetic separation was used for GBS detection.</p></div><div><h3>Materials and methods</h3><p>Sample pre-treatment in this study involved the utilization of magnetic separation (MS) technology, aiming to expedite the detection process and enhance detection sensitivity, and the cfb gene of group B streptococcus was used as the target gene to establish quantitative real-time PCR (RT-qPCR) to detect group B streptococcus.</p></div><div><h3>Results</h3><p>It was found that penicillin-functionalized magnetic beads had a good ability to enrich and capture group B Streptococcus.The findings revealed an exceptional detection sensitivity, with the ability to detect B streptococcus in urine samples at levels as low as 10² CFU/mL.</p></div><div><h3>Conclusions</h3><p>The utilization of MS technology in conjunction with the RT-qPCR (MS-RT-qPCR) assay, as demonstrated in this study, offers a viable approach for prenatal screening of group B streptococcus among perinatal women.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"38 ","pages":"Article e00348"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551723000422/pdfft?md5=cfa7db8d49c4c225775100984b7970fb&pid=1-s2.0-S2352551723000422-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139107800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}