Polish Journal of Microbiology最新文献

{"title":"Lipoic Acid Does Not Affect The Growth of <i>Mycoplasma hominis</i> Cells <i>In Vitro</i>.","authors":"Małgorzata Biernat-Sudolska, Danuta Rojek-Zakrzewska, Paulina Gajda, Anna Bilska-Wilkosz","doi":"10.33073/pjm-2021-050","DOIUrl":"10.33073/pjm-2021-050","url":null,"abstract":"<p><p><i>Mycoplasma hominis</i> is associated with various infections, for which the treatment can be complex. Lipoic acid (LA) plays a role as a cofactor in eukaryotes, most Bacteria, and some Archea. Research of recent years has increasingly pointed to the therapeutic properties of exogenously supplemented LA. The present study was conducted on 40 strains of <i>M. hominis</i> cultured with the following LA concentrations: 1,200 μg/ml, 120 μg/ml, and 12 μg/ml. The bacterial colonies of each strain were counted and expressed as the number of colony-forming units/ml (CFU). The number of CFU in <i>M. hominis</i> strains obtained in the presence of LA was compared with the number of CFU in the strains grown in the media without LA. The obtained results indicated that the presence of LA in the medium did not affect the growth of <i>M. hominis</i>. The investigation of the influence of LA on the growth and survival of microbial cells not only allows for obtaining an answer to the question of whether LA has antimicrobial activity and, therefore, can be used as a drug supporting the treatment of patients infected with a given pathogenic microorganism. Such studies are also crucial for a better understanding of LA metabolism in the microbial cells, which is also important for the search for new antimicrobial drugs. This research is, therefore, an introduction to such further studies.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/c3/pjm-70-4-050.PMC8702601.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39913670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of the Cervicovaginal Microenvironment in Childbearing-Age Women with Different Degrees of Cervical Lesions and HR-HPV Positivity.","authors":"Qingzhi Zhai,&nbsp;Weiyi Zhang,&nbsp;Zhe Zhang,&nbsp;Yurong Fu,&nbsp;Yang Li,&nbsp;Xueqi Wang,&nbsp;Li'an Li,&nbsp;Yuanguang Meng","doi":"10.33073/pjm-2021-046","DOIUrl":"https://doi.org/10.33073/pjm-2021-046","url":null,"abstract":"<p><p>Persistent infection with high-risk human papillomavirus (HR-HPV) is the most important determinate in the development of cervical cancer, and cervical microecology can modulate cervical viral infection. However, few studies have been conducted on the microecological analysis of cervical diseases using strict physiological factors. This study investigated the characteristics and dynamics of cervical microecology in childbearing-age Chinese women with different degrees of HR-HPV-positive cervical lesions. A total of 168 subjects were selected according to the selection criteria, including healthy HPV-negative individuals (n = 29), HR-HPV-infected individuals (n = 29), low-grade squamous intraepithelial lesion individuals (LSIL, n = 32), high-grade squamous intraepithelial lesion individuals (HSIL, n = 40), and cervical cancer individuals (n = 38). We sampled cervical secretions from each subject and performed comparative analysis using the 16S rRNA sequencing method. Comparison analysis showed that <i>Lactobacillus</i> and <i>Ignatzschineria</i> were the dominant genera in the healthy group, while <i>Gardnerella</i> and <i>Prevotella</i> were more enriched in the disease groups. Based on the taxa composition, we roughly divided the development of cervical cancer into two phases: phase I was from healthy status to HR-HPV infection and LSIL; phase II was from LSIL to HSIL and cervical cancer. Different interactions among different genera were observed in different groups. <i>Prevotella</i> inhibited the abundance of <i>Lactobacillus</i> in the healthy group, while <i>Prevotella</i> inhabited the abundance of <i>Gardnerella</i> in the other groups. In the HR-HPV infection group, <i>Ignatzschineria</i> and <i>Enterococcus</i> showed a positive interaction but dissociated with the increase in cervical lesions, which might eventually lead to a continuous decrease in the abundances of <i>Lactobacillus</i> and <i>Ignatzschineria</i>.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/5f/pjm-70-4-046.PMC8702608.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39775314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel LysR Family Factor STM0859 is Associated with The Responses of <i>Salmonella</i> Typhimurium to Environmental Stress and Biofilm Formation.","authors":"Zhongmei Ma,&nbsp;N A Li,&nbsp;Chengcheng Ning,&nbsp;Yucheng Liu,&nbsp;Yun Guo,&nbsp;Chunhui Ji,&nbsp;Xiaozhen Zhu,&nbsp;Qingling Meng,&nbsp;Xianzhu Xia,&nbsp;Xingxing Zhang,&nbsp;Xuepeng Cai,&nbsp;Kuojun Cai,&nbsp;Qiao Jun","doi":"10.33073/pjm-2021-045","DOIUrl":"https://doi.org/10.33073/pjm-2021-045","url":null,"abstract":"<p><p><i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the <i>STM0859</i> gene-deficient strain and the complementation strain <i>ΔSTM0859/STM0859</i> were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene <i>rcsB</i> by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-<i>ΔSTM0859</i> strain were significantly weaker, as compared with those of <i>Salmonella</i> Typhimurium SL1344 (ST-SL1344) wild strain (<i>p</i> < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, <i>rcsB</i> and <i>rpoS</i>, of ST-<i>ΔSTM0859</i> strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-<i>ΔSTM0859</i>/<i>STM0859</i>. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the <i>rcsB</i> gene, suggesting that STM0859 can promote the transcription of the <i>rcsB</i> gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/0e/pjm-70-4-045.PMC8702606.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39913668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silent Genes: Antimicrobial Resistance and Antibiotic Production.","authors":"Monika Stasiak,&nbsp;Elżbieta Maćkiw,&nbsp;Joanna Kowalska,&nbsp;Katarzyna Kucharek,&nbsp;Jacek Postupolski","doi":"10.33073/pjm-2021-040","DOIUrl":"https://doi.org/10.33073/pjm-2021-040","url":null,"abstract":"<p><p>Silent genes are DNA sequences that are generally not expressed or expressed at a very low level. These genes become active as a result of mutation, recombination, or insertion. Silent genes can also be activated in laboratory conditions using pleiotropic, targeted genome-wide, or biosynthetic gene cluster approaches. Like every other gene, silent genes can spread through horizontal gene transfer. Most studies have focused on strains with phenotypic resistance, which is the most common subject. However, to fully understand the mechanism behind the spreading of antibiotic resistance, it is reasonable to study the whole resistome, including silent genes.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2a/82/pjm-70-4-040.PMC8702603.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39677435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can <i>Lactobacillus</i> spp. Be a Factor Reducing the Risk of Miscarriage?","authors":"Aneta Kiecka, Barbara Macura, Marian Szczepanik","doi":"10.33073/pjm-2021-043","DOIUrl":"10.33073/pjm-2021-043","url":null,"abstract":"<p><p>Pregnancy loss is a common obstetric problem. Significant causes of miscarriage include genetic and epigenetic disorders of the embryo, immunological and endocrine factors, uterine malformations, improper embryo selection, and lifestyle. Perhaps a hitherto underappreciated cause of miscarriage may be an abnormal microbiota composition of the female reproductive system. <i>Lactobacillus</i> spp. is the most common bacteria within the reproductive tract. However, the protective role of <i>Lactobacilli</i> in the vagina has been well described in the literature, while it is still unknown what function <i>Lactobacilli</i> may have in the uterus. Moreover, new research shows that <i>Lactobacillus</i> spp. can have a role in miscarriage. However, both molecular and immunological mechanisms of host-<i>Lactobacillus</i> spp. interactions are not fully understood. Understanding these relationships will help address the importance and extent of the protective role of <i>Lactobacillus</i> spp. in miscarriage.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dc/5b/pjm-70-4-043.PMC8702604.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39677436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Components of Aqueous Extracts of <i>Melia azedarach</i> Fruits and Their Effects on The Transcriptome of <i>Staphylococcus aureus</i>.","authors":"Hong Peng,&nbsp;Ying-Si Wang,&nbsp;Jie Wang,&nbsp;Su-Juan Li,&nbsp;Ting-Li Sun,&nbsp;Tong Liu,&nbsp;Qing-Shan Shi,&nbsp;Gang Zhou,&nbsp;Xiao-Bao Xie","doi":"10.33073/pjm-2021-041","DOIUrl":"https://doi.org/10.33073/pjm-2021-041","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of <i>Melia azedarach</i> fruits inhibit the planktonic growth and initial biofilm formation of <i>S. aureus</i> in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after <i>S. aureus</i> exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The majority of DEGs were categorized into abundant sub-groups in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, untargeted UHPLC-MS/MS analyses of the aqueous extracts of <i>M. azedarach</i> fruits demonstrated a highly complex profile in positive and negative electrospray ionization modes. The extracts primarily consisted of lipids and lipid-like molecules, organic acids and their derivatives, phenylpropanoids, polyketides, organoheterocyclic compounds, and benzenoids annotated by abundant lipid maps and KEGG pathways. Overall, this study provides evidences that the aqueous extracts of <i>M. azedarach</i> fruits can control <i>S. aureus</i> infections and sought to understand the mode of action of these extracts on <i>S. aureus</i>.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d7/dc/pjm-70-4-041.PMC8702609.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39913665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QuantiFERON-TB Gold Plus Test in Diagnostics of Latent Tuberculosis Infection in Children Aged 1-14 in a Country with a Low Tuberculosis Incidence.","authors":"Dagmara Borkowska-Tatar,&nbsp;Maria Krasińska,&nbsp;Ewa Augustynowicz-Kopeć","doi":"10.33073/pjm-2021-042","DOIUrl":"https://doi.org/10.33073/pjm-2021-042","url":null,"abstract":"<p><p>The aim of the study was to evaluate the QuantiFERON-TB Gold Plus (QFT-Plus) test usability in the identification of latent tuberculosis infection (LTBI) in children and the determination of features associated with tuberculin skin test (TST) and QFT-Plus-positive results concerning LTBI. Two-hundred thirteen children aged 1-14 were screened for LTBI due to household contact with TB, suspected TB, or were qualified for biological therapy. The objective of this study was to evaluate the QFT-Plus affectivity as a diagnostic test in the absence of a gold standard (GS) test for the diagnosis of LTBI. The children were diagnosed with QFT-Plus, TST, and culture of TB. The QFT-Plus results were analyzed depending on the children's age, TST size, and type. In children aged 1-4, the positive predictive value of QFT-Plus was 1, the negative predictive value was 0.94, QFT-Plus sensitivity was 75%, and specificity was 100%. It was observed that in children aged 5-14 years, the level of agreement decreased to the substantial, i.e., 87.2%. Moreover, the negative predictive value was 0.83. QFT-Plus sensitivity was 64%, and specificity was 100%. Statistical analysis of QFT-Plus and TST results showed substantial and almost perfect agreements. Our study suggests that QFT-Plus is helpful in a pediatric practice showing good sensitivity and specificity for LTBI. The BCG vaccine, infections, and concomitant morbidities do not affect QFT-Plus results.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/07/pjm-70-4-042.PMC8702605.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39913666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Long-Term Storage on <i>Mycobacterium bovis</i>.","authors":"Olexiy Tkachenko,&nbsp;Natali Kozak,&nbsp;Maryna Bilan,&nbsp;Volodymyr Hlebeniuk,&nbsp;Natalia Alekseeva,&nbsp;Liliya Kovaleva,&nbsp;Vitalii Nedosekov,&nbsp;Olexandr Galatiuk","doi":"10.33073/pjm-2021-031","DOIUrl":"https://doi.org/10.33073/pjm-2021-031","url":null,"abstract":"<p><p>It was established that when stored for many years (10-13 years) in low-temperature conditions (3°C), without sub-culture on a nutrient medium, <i>Mycobacterium bovis</i> grew as visible colonies along the line of inoculation. However, due to long-term storage in conditions of low temperature (3°C) morphology of mycobacteria differed significantly from initial cultures formed by rod-shaped bacteria. Some of them became pigment-forming and smooth on the surface. Unlike the initial strain of mycobacteria, a perennial bacteria stored under hard conditions did not cause the death of guinea pigs or their sensitization to a purified protein derivative for mammals. Morphological forms of the perennial mycobacteria had the following changes: pigment forming, L-forms of the vesicular type, non-acid-fast thread-like (filamentous) bacillary forms, and elementary bodies when compared to the initial strain. There were also some genetic changes in the target DNA due to the long-term storage of <i>M. bovis</i>. It may indicate a mutation in the pathogen's DNA. These mycobacteria had altered biochemical activity during storage. The number of passages on the solid nutrient medium did not affect their fermentative activity. However, the low cultivation temperature increases mycobacterial catalase activity and the ability to hydrolyze Tween-80.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ab/48/pjm-70-3-327.PMC8459005.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latent Pathogenic Fungi in the Medicinal Plant <i>Houttuynia cordata</i> Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil.","authors":"Hai-Tao Ye, Shi-Qiong Luo, Zhan-Nan Yang, Yuan-Shuai Wang, Qian Ding","doi":"10.33073/pjm-2021-034","DOIUrl":"10.33073/pjm-2021-034","url":null,"abstract":"<p><p>Latent pathogenic fungi (LPFs) affect plant growth, but some of them may stably colonize plants. LPFs were isolated from healthy <i>Houttuynia cordata</i> rhizomes to reveal this mechanism and identified as <i>Ilyonectria liriodendri</i>, an unidentified fungal sp., and <i>Penicillium citrinum</i>. Sterile <i>H. cordata</i> seedlings were cultivated in sterile or non-sterile soils and inoculated with the LPFs, followed by the plants' analysis. The <i>in vitro</i> antifungal activity of <i>H. cordata</i> rhizome crude extracts on LPF were determined. The effect of inoculation of sterile seedlings by LPFs on the concentrations of rhizome phenolics was evaluated. The rates of <i>in vitro</i> growth inhibition amongst LPFs were determined. The LPFs had a strong negative effect on <i>H. cordata</i> in sterile soil; microbiota in non-sterile soil eliminated such influence. There was an interactive inhibition among LPFs; the secondary metabolites also regulated their colonization in <i>H. cordata</i> rhizomes. LPFs changed the accumulation of phenolics in <i>H. cordata</i>. The results provide that colonization of LPFs in rhizomes was regulated by the colonizing microbiota of <i>H. cordata</i>, the secondary metabolites in the <i>H. cordata</i> rhizomes, and the mutual inhibition and competition between the different latent pathogens.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/7a/pjm-70-3-359.PMC8458996.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Mycobacterium chimaera</i> as an Underestimated Cause of NTM Lung Diseases in Patients Hospitalized in Pulmonary Wards.","authors":"Anna T Zabost,&nbsp;Monika Szturmowicz,&nbsp;Sylwia A Brzezińska,&nbsp;Magdalena D Klatt,&nbsp;Ewa M Augustynowicz-Kopeć","doi":"10.33073/pjm-2021-028","DOIUrl":"https://doi.org/10.33073/pjm-2021-028","url":null,"abstract":"<p><p><i>Mycobacterium chimaera</i> is the newly described species belonging to <i>Mycobacterium avium</i> complex (MAC), with morphology and growth characteristics closely related to <i>Mycobacterium intracellulare</i>. The aim of this retrospective study was to analyze the frequency and clinical significance of <i>M. chimaera</i> identification in the population of patients with previous positive respiratory cultures for <i>M. intracellulare</i> or MAC. 200 strains of <i>M. intracellulare</i> or MAC, isolated from respiratory specimens of patients hospitalized in pulmonary wards, between 2011 and 2020, were retrospectively analyzed with GenoType NTM-DR test. 88 (44%) of strains were re-classified to <i>M. chimaera</i> species. Analysis of clinical data in 30 patients with positive <i>M. chimaera</i> isolates revealed that they were diagnosed with chronic obstructive pulmonary disease (COPD) - 27%, past tuberculosis - 20%, or interstitial lung diseases - 17%, respectively. Non-tuberculous mycobacterial lung disease (NTMLD) caused by <i>M. chimaera</i> has been recognized in 53% of patients, most often in those presenting with post-tuberculous lung lesions. <i>M. chimaera</i> was almost exclusively isolated from respiratory specimens of patients with underlying lung diseases, especially those with COPD and/or past tuberculosis. NTMLD due to <i>M. chimaera</i> was diagnosed predominantly in patients with past tuberculosis.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/ca/pjm-70-3-315.PMC8458994.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}