Polish Journal of Microbiology最新文献_第9页

Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus. 苦楝果实水提物化学成分及其对金黄色葡萄球菌转录组的影响。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-12-01 Epub Date: 2021-12-23 DOI: 10.33073/pjm-2021-041

Hong Peng, Ying-Si Wang, Jie Wang, Su-Juan Li, Ting-Li Sun, Tong Liu, Qing-Shan Shi, Gang Zhou, Xiao-Bao Xie

{"title":"Chemical Components of Aqueous Extracts of Melia azedarach Fruits and Their Effects on The Transcriptome of Staphylococcus aureus.","authors":"Hong Peng, Ying-Si Wang, Jie Wang, Su-Juan Li, Ting-Li Sun, Tong Liu, Qing-Shan Shi, Gang Zhou, Xiao-Bao Xie","doi":"10.33073/pjm-2021-041","DOIUrl":"https://doi.org/10.33073/pjm-2021-041","url":null,"abstract":"Staphylococcus aureus is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of Melia azedarach fruits inhibit the planktonic growth and initial biofilm formation of S. aureus in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after S. aureus exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The majority of DEGs were categorized into abundant sub-groups in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, untargeted UHPLC-MS/MS analyses of the aqueous extracts of M. azedarach fruits demonstrated a highly complex profile in positive and negative electrospray ionization modes. The extracts primarily consisted of lipids and lipid-like molecules, organic acids and their derivatives, phenylpropanoids, polyketides, organoheterocyclic compounds, and benzenoids annotated by abundant lipid maps and KEGG pathways. Overall, this study provides evidences that the aqueous extracts of M. azedarach fruits can control S. aureus infections and sought to understand the mode of action of these extracts on S. aureus.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 4","pages":"447-459"},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d7/dc/pjm-70-4-041.PMC8702609.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39913665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

QuantiFERON-TB Gold Plus Test in Diagnostics of Latent Tuberculosis Infection in Children Aged 1-14 in a Country with a Low Tuberculosis Incidence. QuantiFERON-TB Gold Plus试验在结核病低发病率国家1-14岁儿童潜伏性结核感染诊断中的应用

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-12-01 Epub Date: 2021-12-23 DOI: 10.33073/pjm-2021-042

Dagmara Borkowska-Tatar, Maria Krasińska, Ewa Augustynowicz-Kopeć

{"title":"QuantiFERON-TB Gold Plus Test in Diagnostics of Latent Tuberculosis Infection in Children Aged 1-14 in a Country with a Low Tuberculosis Incidence.","authors":"Dagmara Borkowska-Tatar, Maria Krasińska, Ewa Augustynowicz-Kopeć","doi":"10.33073/pjm-2021-042","DOIUrl":"https://doi.org/10.33073/pjm-2021-042","url":null,"abstract":"The aim of the study was to evaluate the QuantiFERON-TB Gold Plus (QFT-Plus) test usability in the identification of latent tuberculosis infection (LTBI) in children and the determination of features associated with tuberculin skin test (TST) and QFT-Plus-positive results concerning LTBI. Two-hundred thirteen children aged 1-14 were screened for LTBI due to household contact with TB, suspected TB, or were qualified for biological therapy. The objective of this study was to evaluate the QFT-Plus affectivity as a diagnostic test in the absence of a gold standard (GS) test for the diagnosis of LTBI. The children were diagnosed with QFT-Plus, TST, and culture of TB. The QFT-Plus results were analyzed depending on the children's age, TST size, and type. In children aged 1-4, the positive predictive value of QFT-Plus was 1, the negative predictive value was 0.94, QFT-Plus sensitivity was 75%, and specificity was 100%. It was observed that in children aged 5-14 years, the level of agreement decreased to the substantial, i.e., 87.2%. Moreover, the negative predictive value was 0.83. QFT-Plus sensitivity was 64%, and specificity was 100%. Statistical analysis of QFT-Plus and TST results showed substantial and almost perfect agreements. Our study suggests that QFT-Plus is helpful in a pediatric practice showing good sensitivity and specificity for LTBI. The BCG vaccine, infections, and concomitant morbidities do not affect QFT-Plus results.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 4","pages":"461-468"},"PeriodicalIF":2.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/07/pjm-70-4-042.PMC8702605.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39913666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Effect of Long-Term Storage on Mycobacterium bovis. 长期贮藏对牛分枝杆菌的影响。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-031

Olexiy Tkachenko, Natali Kozak, Maryna Bilan, Volodymyr Hlebeniuk, Natalia Alekseeva, Liliya Kovaleva, Vitalii Nedosekov, Olexandr Galatiuk

{"title":"The Effect of Long-Term Storage on Mycobacterium bovis.","authors":"Olexiy Tkachenko, Natali Kozak, Maryna Bilan, Volodymyr Hlebeniuk, Natalia Alekseeva, Liliya Kovaleva, Vitalii Nedosekov, Olexandr Galatiuk","doi":"10.33073/pjm-2021-031","DOIUrl":"https://doi.org/10.33073/pjm-2021-031","url":null,"abstract":"It was established that when stored for many years (10-13 years) in low-temperature conditions (3°C), without sub-culture on a nutrient medium, Mycobacterium bovis grew as visible colonies along the line of inoculation. However, due to long-term storage in conditions of low temperature (3°C) morphology of mycobacteria differed significantly from initial cultures formed by rod-shaped bacteria. Some of them became pigment-forming and smooth on the surface. Unlike the initial strain of mycobacteria, a perennial bacteria stored under hard conditions did not cause the death of guinea pigs or their sensitization to a purified protein derivative for mammals. Morphological forms of the perennial mycobacteria had the following changes: pigment forming, L-forms of the vesicular type, non-acid-fast thread-like (filamentous) bacillary forms, and elementary bodies when compared to the initial strain. There were also some genetic changes in the target DNA due to the long-term storage of M. bovis. It may indicate a mutation in the pathogen's DNA. These mycobacteria had altered biochemical activity during storage. The number of passages on the solid nutrient medium did not affect their fermentative activity. However, the low cultivation temperature increases mycobacterial catalase activity and the ability to hydrolyze Tween-80.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"327-337"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ab/48/pjm-70-3-327.PMC8459005.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil. 药用植物 Houttuynia cordata Thunb.的潜伏病原真菌受次生代谢物和来自土壤的定植微生物群的调节。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-034

Hai-Tao Ye, Shi-Qiong Luo, Zhan-Nan Yang, Yuan-Shuai Wang, Qian Ding

{"title":"Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil.","authors":"Hai-Tao Ye, Shi-Qiong Luo, Zhan-Nan Yang, Yuan-Shuai Wang, Qian Ding","doi":"10.33073/pjm-2021-034","DOIUrl":"10.33073/pjm-2021-034","url":null,"abstract":"Latent pathogenic fungi (LPFs) affect plant growth, but some of them may stably colonize plants. LPFs were isolated from healthy Houttuynia cordata rhizomes to reveal this mechanism and identified as Ilyonectria liriodendri, an unidentified fungal sp., and Penicillium citrinum. Sterile H. cordata seedlings were cultivated in sterile or non-sterile soils and inoculated with the LPFs, followed by the plants' analysis. The in vitro antifungal activity of H. cordata rhizome crude extracts on LPF were determined. The effect of inoculation of sterile seedlings by LPFs on the concentrations of rhizome phenolics was evaluated. The rates of in vitro growth inhibition amongst LPFs were determined. The LPFs had a strong negative effect on H. cordata in sterile soil; microbiota in non-sterile soil eliminated such influence. There was an interactive inhibition among LPFs; the secondary metabolites also regulated their colonization in H. cordata rhizomes. LPFs changed the accumulation of phenolics in H. cordata. The results provide that colonization of LPFs in rhizomes was regulated by the colonizing microbiota of H. cordata, the secondary metabolites in the H. cordata rhizomes, and the mutual inhibition and competition between the different latent pathogens.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"359-372"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/7a/pjm-70-3-359.PMC8458996.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium chimaera as an Underestimated Cause of NTM Lung Diseases in Patients Hospitalized in Pulmonary Wards. 分枝杆菌嵌合是肺部病房住院患者NTM肺病的一个被低估的病因。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-028

Anna T Zabost, Monika Szturmowicz, Sylwia A Brzezińska, Magdalena D Klatt, Ewa M Augustynowicz-Kopeć

{"title":"Mycobacterium chimaera as an Underestimated Cause of NTM Lung Diseases in Patients Hospitalized in Pulmonary Wards.","authors":"Anna T Zabost, Monika Szturmowicz, Sylwia A Brzezińska, Magdalena D Klatt, Ewa M Augustynowicz-Kopeć","doi":"10.33073/pjm-2021-028","DOIUrl":"https://doi.org/10.33073/pjm-2021-028","url":null,"abstract":"Mycobacterium chimaera is the newly described species belonging to Mycobacterium avium complex (MAC), with morphology and growth characteristics closely related to Mycobacterium intracellulare. The aim of this retrospective study was to analyze the frequency and clinical significance of M. chimaera identification in the population of patients with previous positive respiratory cultures for M. intracellulare or MAC. 200 strains of M. intracellulare or MAC, isolated from respiratory specimens of patients hospitalized in pulmonary wards, between 2011 and 2020, were retrospectively analyzed with GenoType NTM-DR test. 88 (44%) of strains were re-classified to M. chimaera species. Analysis of clinical data in 30 patients with positive M. chimaera isolates revealed that they were diagnosed with chronic obstructive pulmonary disease (COPD) - 27%, past tuberculosis - 20%, or interstitial lung diseases - 17%, respectively. Non-tuberculous mycobacterial lung disease (NTMLD) caused by M. chimaera has been recognized in 53% of patients, most often in those presenting with post-tuberculous lung lesions. M. chimaera was almost exclusively isolated from respiratory specimens of patients with underlying lung diseases, especially those with COPD and/or past tuberculosis. NTMLD due to M. chimaera was diagnosed predominantly in patients with past tuberculosis.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"315-320"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/ca/pjm-70-3-315.PMC8458994.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Transcriptome Analysis of Komagataeibacter europaeus CGMCC 20445 Responses to Different Acidity Levels During Acetic Acid Fermentation. Komagataeibacter europaeus CGMCC 20445 在醋酸发酵过程中对不同酸度反应的转录组分析。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-027

Liting Wang, Housheng Hong, Chengbo Zhang, Zunxi Huang, Huiming Guo

{"title":"Transcriptome Analysis of Komagataeibacter europaeus CGMCC 20445 Responses to Different Acidity Levels During Acetic Acid Fermentation.","authors":"Liting Wang, Housheng Hong, Chengbo Zhang, Zunxi Huang, Huiming Guo","doi":"10.33073/pjm-2021-027","DOIUrl":"10.33073/pjm-2021-027","url":null,"abstract":"In the industrial production of high-acidity vinegar, the initial ethanol and acetic acid concentrations are limiting factors that will affect acetic acid fermentation. In this study, Komagataeibacter europaeus CGMCC 20445 was used for acetic acid shake flask fermentation at an initial ethanol concentration of 4.3% (v/v). We conducted transcriptome analysis of K. europaeus CGMCC 20445 samples under different acidity conditions to elucidate the changes in differentially expressed genes throughout the fermentation process. We also analyzed the expression of genes associated with acid-resistance mechanisms. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the differentially expressed genes were enriched in ribosomes, citrate cycle, butanoate metabolism, oxidative phosphorylation, pentose phosphate, and the fatty acid biosynthetic pathways. In addition, this study found that K. europaeus CGMCC 20445 regulates the gene expression levels of cell envelope proteins and stress-responsive proteins to adapt to the gradual increase in acidity during acetic acid fermentation. This study improved the understanding of the acid resistance mechanism of K. europaeus and provided relevant reference information for the further genetic engineering of this bacterium.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"305-313"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3a/a9/pjm-70-3-305.PMC8459000.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Full Genome Characterization of SARS-CoV-2 in Stool Specimen from a Recovered Patient, China. 中国一名康复患者粪便标本中SARS-CoV-2的发现和全基因组表征

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-029

Yongdong Li, Y I Chen, Hongxia Ni, B O Yi, Dandan Zhang, Jianing Zhang, Wenjing Wang, Yuhui Liu, Suli Jiao, Guozhang Xu, Weidong Qian

引用次数: 1

Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water. 利用超声波减少水中大肠弯曲杆菌的数量。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-032

Marek Selwet

{"title":"Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water.","authors":"Marek Selwet","doi":"10.33073/pjm-2021-032","DOIUrl":"https://doi.org/10.33073/pjm-2021-032","url":null,"abstract":"The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate Campylobacter spp. from water. The strains used in this research were isolated from water contaminated with sewage. Campylobacter coli alone was detected in the samples and used for further research. The reference strain C. coli ATCC 33559 was simultaneously tested. The isolate was exposed to ultrasounds at frequencies of 37 kHz and 80 kHz in a continuous operation device with ultrapure deionized water. After 5 min of sonication, the count of C. coli decreased by 5.78% (37 kHz) and 6.27% (80 kHz), whereas the temperature increased by 3°C (37 kHz), and 6°C (80 kHz). After 30 min of sonication, the death rates of bacterial cells were 40.15% (37 kHz) and 55.10% (80 kHz), whereas the temperature reached the maximum values of 36°C (37 kHz), and 39°C (80 kHz). Sonication at the frequency of 80 kHz reduced the bacterial count from 6.86 log CFU/ml to 3.08 log CFU/ml, whereas the frequency of 37 kHz reduced the bacterial count from 6.75 log CFU/ml to 4.04 log CFU/ml. Despite significant differences (p < 0.05) in the number of C. coli cells, the cell death rate remained at the same level.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"339-343"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cc/8e/pjm-70-3-339.PMC8458995.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test. 改良联合圆盘法快速检测和鉴别肠杆菌分离株中KPC和MBL碳青霉烯酶。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-036

Ming Wei, Peng Wang, Shuai Wang, Chunxia Yang, L I Gu

{"title":"Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test.","authors":"Ming Wei, Peng Wang, Shuai Wang, Chunxia Yang, L I Gu","doi":"10.33073/pjm-2021-036","DOIUrl":"https://doi.org/10.33073/pjm-2021-036","url":null,"abstract":"This study was conducted to develop a cheap, rapid, and accurate modified combined-disk test (mCDT) approach to detect and differentiate KPC and MBL carbapenemases among clinical carbapenem-resistant Enterobacterales (CRE) isolates and simultaneously distinguish them from carbapenem-susceptible Enterobacterales (CSE) isolates. A total of 163 CRE and 90 third-generation cephalosporin-resistant Enterobacterales isolates were tested using imipenem and meropenem disks and different concentrations of carbapenemase inhibitors. The optimal sensitivity and specificity for detecting KPC carbapenemase were 97.2% and 100%, respectively. The sensitivity and specificity for detecting MBL carbapenemase were 100% and 100% with imipenem or meropenem and carbapenemase inhibitors within six hours. The inhibitory zone diameter of 18 mm for imipenem or meropenem disks without inhibitor could distinguish CRE from CSE isolates. Therefore, this mCDT approach may be a useful tool in clinical laboratories to detect CRE isolates and differentiate KPC and MBL producers, which is beneficial for patient management and hospital infection prevention and control.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"387-394"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/45/pjm-70-3-387.PMC8458999.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fungal Infections in COVID-19 Intensive Care Patients. COVID-19 重症监护患者的真菌感染。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-039

Ayşenur Sümer Coşkun, Şenay Öztürk Durmaz

{"title":"Fungal Infections in COVID-19 Intensive Care Patients.","authors":"Ayşenur Sümer Coşkun, Şenay Öztürk Durmaz","doi":"10.33073/pjm-2021-039","DOIUrl":"10.33073/pjm-2021-039","url":null,"abstract":"Opportunistic fungal infections increase morbidity and mortality in COVID-19 patients monitored in intensive care units (ICU). As patients' hospitalization days in the ICU and intubation period increase, opportunistic infections also increase, which prolongs hospital stay days and elevates costs. The study aimed to describe the profile of fungal infections and identify the risk factors associated with mortality in COVID-19 intensive care patients. The records of 627 patients hospitalized in ICU with the diagnosis of COVID-19 were investigated from electronic health records and hospitalization files. The demographic characteristics (age, gender), the number of ICU hospitalization days and mortality rates, APACHE II scores, accompanying diseases, antibiotic-steroid treatments taken during hospitalization, and microbiological results (blood, urine, tracheal aspirate samples) of the patients were recorded. Opportunistic fungal infection was detected in 32 patients (5.10%) of 627 patients monitored in ICU with a COVID-19 diagnosis. The average APACHE II score of the patients was 28 ± 6. While 25 of the patients (78.12%) died, seven (21.87%) were discharged from the ICU. Candida parapsilosis (43.7%) was the opportunistic fungal agent isolated from most blood samples taken from COVID-19 positive patients. The mortality rate of COVID-19 positive patients with candidemia was 80%. While two out of the three patients (66.6%) for whom fungi were grown from their tracheal aspirate died, one patient (33.3%) was transferred to the ward. Opportunistic fungal infections increase the mortality rate of COVID-19-positive patients. In addition to the risk factors that we cannot change, invasive procedures should be avoided, constant blood sugar regulation should be applied, and unnecessary antibiotics use should be avoided.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"395-400"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/c5/pjm-70-3-395.PMC8459001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0