Polish Journal of Microbiology最新文献_第10页

Clinical Characteristics of Patients with Micrococcus luteus Bloodstream Infection in a Chinese Tertiary-Care Hospital. 某三级医院黄体微球菌血流感染的临床特点

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-030

Minghui Zhu, Qiang Zhu, Zhen Yang, Zhixin Liang

{"title":"Clinical Characteristics of Patients with Micrococcus luteus Bloodstream Infection in a Chinese Tertiary-Care Hospital.","authors":"Minghui Zhu, Qiang Zhu, Zhen Yang, Zhixin Liang","doi":"10.33073/pjm-2021-030","DOIUrl":"https://doi.org/10.33073/pjm-2021-030","url":null,"abstract":"Few pieces of research have focused on Micrococcus luteus bloodstream infection (BSI) because of its low incidence; hence data is needed to illustrate this uncommon infection. This study aimed to explore the clinical characteristics of patients with M. luteus BSI. From January 2010 to December 2019, inpatients that met the criteria for M. luteus BSI were included in this study. Data was collected by reviewing electronic records. Ninety-seven patients were enrolled in this study. Sixty-three percent of the patients have a higher neutrophil percentage (NEUT%). The average blood C-reactive protein (CRP) concentration was 5.5 ± 6.4 mg/dl. 48.5% of the patients had malignancy, and 40.2% underwent invasive surgeries. Linezolid was found to have the largest average diameter of the inhibition zone (36 mm), while erythromycin was found to have the smallest average zone diameter (15 mm). However, some M. luteus strains had a potentially broad antimicrobial resistance spectrum. Cephalosporins (59.2%) and quinolones (21.4%) were the most commonly used antibiotics for empirical therapies. In conclusion, M. luteus BSI mainly happens in immunocompromised patients or those with former invasive surgeries or indwelling catheters. M. luteus strains are less responsive to erythromycin. Cephalosporins and quinolones are effective empirical antibiotics for M. luteus BSI; however, vancomycin and teicoplanin should be considered for potentially broadly drug-resistant M. luteus strains.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"321-326"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ca/c6/pjm-70-3-321.PMC8459002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Characteristics of Vaginal Microbiome in Women with Pelvic Inflammatory Disease in Korea. 韩国女性盆腔炎患者阴道微生物组的特征

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-033

Sukyung Kim, Hoonhee Seo, Md Abdur Rahim, Hanieh Tajdozian, Yun-Sook Kim, Ho-Yeon Song

{"title":"Characteristics of Vaginal Microbiome in Women with Pelvic Inflammatory Disease in Korea.","authors":"Sukyung Kim, Hoonhee Seo, Md Abdur Rahim, Hanieh Tajdozian, Yun-Sook Kim, Ho-Yeon Song","doi":"10.33073/pjm-2021-033","DOIUrl":"https://doi.org/10.33073/pjm-2021-033","url":null,"abstract":"Human vaginal microorganisms play an important role in maintaining good health throughout the human life cycle. An imbalance in the vaginal microbiota is associated with an increased risk of pelvic inflammatory disease (PID). This study aimed to characterize and compare vaginal microbial profiles of premenopausal Korean women with and without PID. 74 Korean premenopausal female vaginal samples were obtained; 33 were from healthy women (a control group) and 41 from PID patients. Vaginal fluid samples were collected from the vaginal wall and posterior cervix and then analyzed by 16S ribosomal ribonucleic acid (rRNA) gene-based amplicon sequencing. Results showed a significant difference between the vaginal microbial communities of the two groups (Jensen-Shannon, p = 0.014; Bray-Curtis, p = 0.009; Generalized UniFrac, p = 0.007; UniFrac, p = 0.008). Lactobacillus accounted for the highest percentage (61.0%) of the control group but was significantly decreased (34.9%) in PID patients; this was the most significant difference among all bacterial communities (p = 0.028, LDA effect size = 5.129). In addition, in the PID patient group, species diversity significantly increased (Simpson, p = 0.07) as the proportion of various pathogens increased evenly, resulting in a polymicrobial infection. Similarly, lactate, which constituted the highest percentage of the organic acids in the control group, was significantly decreased in the PID patient group (p = 0.04). The present study's findings will help understand PID from the microbiome perspective and are expected to contribute to the development of more efficient PID diagnosis and treatment modalities.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"345-357"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/07/pjm-70-3-345.PMC8458998.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

First Isolation of Exiguobacterium aurantiacum in Serbia. 塞尔维亚首次分离到金吸出口杆菌。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-037

Vera P Gusman, Deana D Medić, Anika Dj Trudić, Pavle Z Banović, Nataša M Nikolić

引用次数: 0

Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella aerogenes. 支气管镜相关产气克雷伯氏菌的比较基因组分析和表型特征。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-038

Fang Huang, Shuang Li, Lan Lou, Junjun Mo, Hao Xu

引用次数: 0

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants. 某些禾本科植物内生细菌产生水解酶的研究。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-026

Gokhan Dogan, Bilgin Taskin

{"title":"Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants.","authors":"Gokhan Dogan, Bilgin Taskin","doi":"10.33073/pjm-2021-026","DOIUrl":"https://doi.org/10.33073/pjm-2021-026","url":null,"abstract":"Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium, and Bacillus genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"297-304"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/82/pjm-70-3-297.PMC8456377.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Bacterial Community Analysis and Potential Functions of Core Taxa in Different Parts of the Fungus Cantharellus cibarius. 中国刺参真菌不同部位核心类群的细菌群落分析及潜在功能。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-035

Wei Ge, Zhi-Yuan Zhang, Chun-Bo Dong, Yan-Feng Han, Sunil K Deshmukh, Zong-Qi Liang

{"title":"Bacterial Community Analysis and Potential Functions of Core Taxa in Different Parts of the Fungus Cantharellus cibarius.","authors":"Wei Ge, Zhi-Yuan Zhang, Chun-Bo Dong, Yan-Feng Han, Sunil K Deshmukh, Zong-Qi Liang","doi":"10.33073/pjm-2021-035","DOIUrl":"https://doi.org/10.33073/pjm-2021-035","url":null,"abstract":"Cantharellus cibarius is a widely distributed, popular, edible fungus with high nutritional and economic value. However, significant challenges persist in the microbial ecology and artificial cultivation of C. cibarius. Based on the 16S rRNA sequencing data, this study analyzed bacterial community structures and diversity of fruit bodies and rhizomorph parts of C. cibarius and mycosphere samples (collected in the Wudang District, Guiyang, Guizhou Province, China). It explored the composition and function of the core bacterial taxa. The analyzed results showed that the rhizomorph bacterial community structure was similar to mycosphere, but differed from the fruit bodies. Members of the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium complex had the highest abundance in the fruit bodies. However, they were either absent or low in abundance in the rhizomorphs and mycosphere. At the same time, members of the Burkholderia-Caballeronia-Paraburkholderia complex were abundant in the fruit bodies and rhizomorphs parts of C. cibarius, as well as mycosphere. Through functional annotation of core bacterial taxa, we found that there was an apparent trend of potential functional differentiation of related bacterial communities in the fruit body and rhizomorph: potential functional groups of core bacterial taxa in the fruit bodies centered on nitrogen fixation, nitrogen metabolism, and degradation of aromatic compounds, while those in rhizomorphs focused on aerobic chemoheterotrophy, chemoheterotrophy, defense against soil pathogens, decomposition of complex organic compounds, and uptake of insoluble inorganic compounds. The analysis of functional groups of bacteria with different structures is of great significance to understand that bacteria promote the growth and development of C. cibarius.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"373-385"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/02/pjm-70-3-373.PMC8459004.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing. 16S rRNA扩增子测序分析植物乳杆菌BW2013对小鼠肠道菌群的影响

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-06-01 Epub Date: 2021-06-21 DOI: 10.33073/pjm-2021-022

Tong Tong, Xiaohui Niu, Qian Li, Yuxi Ling, Zuming Li, Jia Liu, Michael Zhang, Zhihui Bai, Ran Xia, Zhichao Wu, Xiu Liu

{"title":"The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing.","authors":"Tong Tong, Xiaohui Niu, Qian Li, Yuxi Ling, Zuming Li, Jia Liu, Michael Zhang, Zhihui Bai, Ran Xia, Zhichao Wu, Xiu Liu","doi":"10.33073/pjm-2021-022","DOIUrl":"https://doi.org/10.33073/pjm-2021-022","url":null,"abstract":"Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 108 CFU/ml, a medium-dose group of 109 CFU/ml, and a high-dose group of 1010 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 2","pages":"235-243"},"PeriodicalIF":2.1,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/4b/pjm-70-2-022.PMC8326986.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39275992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Emerging Applications of Bacteriocins as Antimicrobials, Anticancer Drugs, and Modulators of The Gastrointestinal Microbiota. 细菌素作为抗菌剂、抗癌药物和胃肠道微生物群调节剂的新兴应用。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-06-01 Epub Date: 2021-06-21 DOI: 10.33073/pjm-2021-020

Catherine Cesa-Luna, Julia-María Alatorre-Cruz, Ricardo Carreño-López, Verónica Quintero-Hernández, Antonino Baez

引用次数: 0

First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia. 突尼斯污染场地中分离的对重金属和抗生素耐药的各种细菌的首次描述。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-06-01 Epub Date: 2021-06-21 DOI: 10.33073/pjm-2021-012

Samar Ben Miloud, Olfa Dziri, Sana Ferjani, Muntasir Md Ali, Mohamed Mysara, Ilhem Boutiba, Rob VAN Houdt, Chedly Chouchani

{"title":"First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia.","authors":"Samar Ben Miloud, Olfa Dziri, Sana Ferjani, Muntasir Md Ali, Mohamed Mysara, Ilhem Boutiba, Rob VAN Houdt, Chedly Chouchani","doi":"10.33073/pjm-2021-012","DOIUrl":"https://doi.org/10.33073/pjm-2021-012","url":null,"abstract":"Environmental bacteria belonging to various families were isolated from polluted water collected from ten different sites in Tunisia. Sites were chosen near industrial and urban areas known for their high degree of pollution. The aim of this study was to investigate cross-resistance between heavy metals (HM), i.e., silver, mercury and copper (Ag, Hg, and Cu), and antibiotics. In an initial screening, 80 isolates were selected on ampicillin, and 39 isolates, retained for further analysis, could grow on a Tris-buffered mineral medium with gluconate as carbon source. Isolates were identified based on their 16S rRNA gene sequence. Results showed the prevalence of antibiotic resistance genes, especially all isolates harbored the bla TEM gene. Some of them (15.38%) harbored bla SHV. Moreover, several were even ESBLs and MBLs-producers, which can threaten the human health. On the other hand, 92.30%, 56.41%, and 51.28% of the isolates harbored the heavy metals resistance genes silE, cusA, and merA, respectively. These genes confer resistance to silver, copper, and mercury. A cross-resistance between antibiotics and heavy metals was detected in 97.43% of our isolates.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 2","pages":"161-174"},"PeriodicalIF":2.1,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/60/pjm-70-2-012.PMC8318066.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39266722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Environmental Factors Associated with the Eukaryotic Microbial Community and Microalgal Groups in the Mountain Marshes of South Korea. 与韩国山地沼泽真核微生物群落和微藻群相关的环境因素。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-06-01 Epub Date: 2021-06-21 DOI: 10.33073/pjm-2021-019

Young-Saeng Kim, Hyun-Sik Yun, Jea Hack Lee, Han-Soon Kim, Ho-Sung Yoon

{"title":"Environmental Factors Associated with the Eukaryotic Microbial Community and Microalgal Groups in the Mountain Marshes of South Korea.","authors":"Young-Saeng Kim, Hyun-Sik Yun, Jea Hack Lee, Han-Soon Kim, Ho-Sung Yoon","doi":"10.33073/pjm-2021-019","DOIUrl":"https://doi.org/10.33073/pjm-2021-019","url":null,"abstract":"The diversity indices of eukaryotic microalgal groups in the Jeonglyeongchi, Waegok, and Wangdeungjae marshes of Mount Jiri, Korea, were measured using Illumina MiSeq and culture-based analyses. Waegok marsh had the highest species richness, with a Chao1 value of 828.00, and the highest levels of species diversity, with Shannon and Simpson index values of 6.36 and 0.94, respectively, while Wangdeungjae marsh had the lowest values at 2.97 and 0.75, respectively. The predominant species in all communities were Phagocata sibirica (Jeonglyeongchi, 68.64%), Aedes albopictus (Waegok, 34.77%), Chaetonotus cf. (Waegok, 24.43%), Eimeria sp. (Wangdeungjae, 26.17%), and Eumonhystera cf. (Wangdeungjae, 22.27%). Relative abundances of the microalgal groups Bacillariophyta (diatoms) and Chlorophyta (green algae) in each marsh were respectively: Jeonglyeongchi 1.38% and 0.49%, Waegok 7.0% and 0.3%, and Wangdeungjae 10.41% and 4.72%. Illumina MiSeq analyses revealed 34 types of diatoms and 13 types of green algae. Only one diatom (Nitzschia dissipata) and five green algae (Neochloris sp., Chlamydomonas sp., Chlorococcum sp., Chlorella vulgaris, Scenedesmus sp.) were identified by a culture-based analysis. Thus, Illumina MiSeq analysis can be considered an efficient tool for analyzing microbial communities. Overall, our results described the environmental factors associated with geographically isolated mountain marshes and their respective microbial and microalgal communities.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 2","pages":"215-233"},"PeriodicalIF":2.1,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/a4/pjm-70-2-019.PMC8326984.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39278100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1