Polish Journal of Microbiology最新文献_第10页

Transcriptome Analysis of Komagataeibacter europaeus CGMCC 20445 Responses to Different Acidity Levels During Acetic Acid Fermentation. Komagataeibacter europaeus CGMCC 20445 在醋酸发酵过程中对不同酸度反应的转录组分析。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-027

Liting Wang, Housheng Hong, Chengbo Zhang, Zunxi Huang, Huiming Guo

{"title":"Transcriptome Analysis of Komagataeibacter europaeus CGMCC 20445 Responses to Different Acidity Levels During Acetic Acid Fermentation.","authors":"Liting Wang, Housheng Hong, Chengbo Zhang, Zunxi Huang, Huiming Guo","doi":"10.33073/pjm-2021-027","DOIUrl":"10.33073/pjm-2021-027","url":null,"abstract":"In the industrial production of high-acidity vinegar, the initial ethanol and acetic acid concentrations are limiting factors that will affect acetic acid fermentation. In this study, Komagataeibacter europaeus CGMCC 20445 was used for acetic acid shake flask fermentation at an initial ethanol concentration of 4.3% (v/v). We conducted transcriptome analysis of K. europaeus CGMCC 20445 samples under different acidity conditions to elucidate the changes in differentially expressed genes throughout the fermentation process. We also analyzed the expression of genes associated with acid-resistance mechanisms. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the differentially expressed genes were enriched in ribosomes, citrate cycle, butanoate metabolism, oxidative phosphorylation, pentose phosphate, and the fatty acid biosynthetic pathways. In addition, this study found that K. europaeus CGMCC 20445 regulates the gene expression levels of cell envelope proteins and stress-responsive proteins to adapt to the gradual increase in acidity during acetic acid fermentation. This study improved the understanding of the acid resistance mechanism of K. europaeus and provided relevant reference information for the further genetic engineering of this bacterium.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"305-313"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3a/a9/pjm-70-3-305.PMC8459000.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Full Genome Characterization of SARS-CoV-2 in Stool Specimen from a Recovered Patient, China. 中国一名康复患者粪便标本中SARS-CoV-2的发现和全基因组表征

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-029

Yongdong Li, Y I Chen, Hongxia Ni, B O Yi, Dandan Zhang, Jianing Zhang, Wenjing Wang, Yuhui Liu, Suli Jiao, Guozhang Xu, Weidong Qian

引用次数: 1

Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water. 利用超声波减少水中大肠弯曲杆菌的数量。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-032

Marek Selwet

{"title":"Use of Ultrasounds to Reduce the Count of Campylobacter coli in Water.","authors":"Marek Selwet","doi":"10.33073/pjm-2021-032","DOIUrl":"https://doi.org/10.33073/pjm-2021-032","url":null,"abstract":"The present study aimed to evaluate the effectiveness of low-frequency ultrasounds applied to eliminate Campylobacter spp. from water. The strains used in this research were isolated from water contaminated with sewage. Campylobacter coli alone was detected in the samples and used for further research. The reference strain C. coli ATCC 33559 was simultaneously tested. The isolate was exposed to ultrasounds at frequencies of 37 kHz and 80 kHz in a continuous operation device with ultrapure deionized water. After 5 min of sonication, the count of C. coli decreased by 5.78% (37 kHz) and 6.27% (80 kHz), whereas the temperature increased by 3°C (37 kHz), and 6°C (80 kHz). After 30 min of sonication, the death rates of bacterial cells were 40.15% (37 kHz) and 55.10% (80 kHz), whereas the temperature reached the maximum values of 36°C (37 kHz), and 39°C (80 kHz). Sonication at the frequency of 80 kHz reduced the bacterial count from 6.86 log CFU/ml to 3.08 log CFU/ml, whereas the frequency of 37 kHz reduced the bacterial count from 6.75 log CFU/ml to 4.04 log CFU/ml. Despite significant differences (p < 0.05) in the number of C. coli cells, the cell death rate remained at the same level.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"339-343"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cc/8e/pjm-70-3-339.PMC8458995.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test. 改良联合圆盘法快速检测和鉴别肠杆菌分离株中KPC和MBL碳青霉烯酶。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-036

Ming Wei, Peng Wang, Shuai Wang, Chunxia Yang, L I Gu

{"title":"Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test.","authors":"Ming Wei, Peng Wang, Shuai Wang, Chunxia Yang, L I Gu","doi":"10.33073/pjm-2021-036","DOIUrl":"https://doi.org/10.33073/pjm-2021-036","url":null,"abstract":"This study was conducted to develop a cheap, rapid, and accurate modified combined-disk test (mCDT) approach to detect and differentiate KPC and MBL carbapenemases among clinical carbapenem-resistant Enterobacterales (CRE) isolates and simultaneously distinguish them from carbapenem-susceptible Enterobacterales (CSE) isolates. A total of 163 CRE and 90 third-generation cephalosporin-resistant Enterobacterales isolates were tested using imipenem and meropenem disks and different concentrations of carbapenemase inhibitors. The optimal sensitivity and specificity for detecting KPC carbapenemase were 97.2% and 100%, respectively. The sensitivity and specificity for detecting MBL carbapenemase were 100% and 100% with imipenem or meropenem and carbapenemase inhibitors within six hours. The inhibitory zone diameter of 18 mm for imipenem or meropenem disks without inhibitor could distinguish CRE from CSE isolates. Therefore, this mCDT approach may be a useful tool in clinical laboratories to detect CRE isolates and differentiate KPC and MBL producers, which is beneficial for patient management and hospital infection prevention and control.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"387-394"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a3/45/pjm-70-3-387.PMC8458999.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fungal Infections in COVID-19 Intensive Care Patients. COVID-19 重症监护患者的真菌感染。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-039

Ayşenur Sümer Coşkun, Şenay Öztürk Durmaz

{"title":"Fungal Infections in COVID-19 Intensive Care Patients.","authors":"Ayşenur Sümer Coşkun, Şenay Öztürk Durmaz","doi":"10.33073/pjm-2021-039","DOIUrl":"10.33073/pjm-2021-039","url":null,"abstract":"Opportunistic fungal infections increase morbidity and mortality in COVID-19 patients monitored in intensive care units (ICU). As patients' hospitalization days in the ICU and intubation period increase, opportunistic infections also increase, which prolongs hospital stay days and elevates costs. The study aimed to describe the profile of fungal infections and identify the risk factors associated with mortality in COVID-19 intensive care patients. The records of 627 patients hospitalized in ICU with the diagnosis of COVID-19 were investigated from electronic health records and hospitalization files. The demographic characteristics (age, gender), the number of ICU hospitalization days and mortality rates, APACHE II scores, accompanying diseases, antibiotic-steroid treatments taken during hospitalization, and microbiological results (blood, urine, tracheal aspirate samples) of the patients were recorded. Opportunistic fungal infection was detected in 32 patients (5.10%) of 627 patients monitored in ICU with a COVID-19 diagnosis. The average APACHE II score of the patients was 28 ± 6. While 25 of the patients (78.12%) died, seven (21.87%) were discharged from the ICU. Candida parapsilosis (43.7%) was the opportunistic fungal agent isolated from most blood samples taken from COVID-19 positive patients. The mortality rate of COVID-19 positive patients with candidemia was 80%. While two out of the three patients (66.6%) for whom fungi were grown from their tracheal aspirate died, one patient (33.3%) was transferred to the ward. Opportunistic fungal infections increase the mortality rate of COVID-19-positive patients. In addition to the risk factors that we cannot change, invasive procedures should be avoided, constant blood sugar regulation should be applied, and unnecessary antibiotics use should be avoided.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"395-400"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/c5/pjm-70-3-395.PMC8459001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Characteristics of Patients with Micrococcus luteus Bloodstream Infection in a Chinese Tertiary-Care Hospital. 某三级医院黄体微球菌血流感染的临床特点

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-030

Minghui Zhu, Qiang Zhu, Zhen Yang, Zhixin Liang

{"title":"Clinical Characteristics of Patients with Micrococcus luteus Bloodstream Infection in a Chinese Tertiary-Care Hospital.","authors":"Minghui Zhu, Qiang Zhu, Zhen Yang, Zhixin Liang","doi":"10.33073/pjm-2021-030","DOIUrl":"https://doi.org/10.33073/pjm-2021-030","url":null,"abstract":"Few pieces of research have focused on Micrococcus luteus bloodstream infection (BSI) because of its low incidence; hence data is needed to illustrate this uncommon infection. This study aimed to explore the clinical characteristics of patients with M. luteus BSI. From January 2010 to December 2019, inpatients that met the criteria for M. luteus BSI were included in this study. Data was collected by reviewing electronic records. Ninety-seven patients were enrolled in this study. Sixty-three percent of the patients have a higher neutrophil percentage (NEUT%). The average blood C-reactive protein (CRP) concentration was 5.5 ± 6.4 mg/dl. 48.5% of the patients had malignancy, and 40.2% underwent invasive surgeries. Linezolid was found to have the largest average diameter of the inhibition zone (36 mm), while erythromycin was found to have the smallest average zone diameter (15 mm). However, some M. luteus strains had a potentially broad antimicrobial resistance spectrum. Cephalosporins (59.2%) and quinolones (21.4%) were the most commonly used antibiotics for empirical therapies. In conclusion, M. luteus BSI mainly happens in immunocompromised patients or those with former invasive surgeries or indwelling catheters. M. luteus strains are less responsive to erythromycin. Cephalosporins and quinolones are effective empirical antibiotics for M. luteus BSI; however, vancomycin and teicoplanin should be considered for potentially broadly drug-resistant M. luteus strains.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"321-326"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ca/c6/pjm-70-3-321.PMC8459002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Characteristics of Vaginal Microbiome in Women with Pelvic Inflammatory Disease in Korea. 韩国女性盆腔炎患者阴道微生物组的特征

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-033

Sukyung Kim, Hoonhee Seo, Md Abdur Rahim, Hanieh Tajdozian, Yun-Sook Kim, Ho-Yeon Song

{"title":"Characteristics of Vaginal Microbiome in Women with Pelvic Inflammatory Disease in Korea.","authors":"Sukyung Kim, Hoonhee Seo, Md Abdur Rahim, Hanieh Tajdozian, Yun-Sook Kim, Ho-Yeon Song","doi":"10.33073/pjm-2021-033","DOIUrl":"https://doi.org/10.33073/pjm-2021-033","url":null,"abstract":"Human vaginal microorganisms play an important role in maintaining good health throughout the human life cycle. An imbalance in the vaginal microbiota is associated with an increased risk of pelvic inflammatory disease (PID). This study aimed to characterize and compare vaginal microbial profiles of premenopausal Korean women with and without PID. 74 Korean premenopausal female vaginal samples were obtained; 33 were from healthy women (a control group) and 41 from PID patients. Vaginal fluid samples were collected from the vaginal wall and posterior cervix and then analyzed by 16S ribosomal ribonucleic acid (rRNA) gene-based amplicon sequencing. Results showed a significant difference between the vaginal microbial communities of the two groups (Jensen-Shannon, p = 0.014; Bray-Curtis, p = 0.009; Generalized UniFrac, p = 0.007; UniFrac, p = 0.008). Lactobacillus accounted for the highest percentage (61.0%) of the control group but was significantly decreased (34.9%) in PID patients; this was the most significant difference among all bacterial communities (p = 0.028, LDA effect size = 5.129). In addition, in the PID patient group, species diversity significantly increased (Simpson, p = 0.07) as the proportion of various pathogens increased evenly, resulting in a polymicrobial infection. Similarly, lactate, which constituted the highest percentage of the organic acids in the control group, was significantly decreased in the PID patient group (p = 0.04). The present study's findings will help understand PID from the microbiome perspective and are expected to contribute to the development of more efficient PID diagnosis and treatment modalities.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"345-357"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/07/pjm-70-3-345.PMC8458998.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

First Isolation of Exiguobacterium aurantiacum in Serbia. 塞尔维亚首次分离到金吸出口杆菌。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-037

Vera P Gusman, Deana D Medić, Anika Dj Trudić, Pavle Z Banović, Nataša M Nikolić

引用次数: 0

Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella aerogenes. 支气管镜相关产气克雷伯氏菌的比较基因组分析和表型特征。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-038

Fang Huang, Shuang Li, Lan Lou, Junjun Mo, Hao Xu

引用次数: 0

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants. 某些禾本科植物内生细菌产生水解酶的研究。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2021-09-01 Epub Date: 2021-09-17 DOI: 10.33073/pjm-2021-026

Gokhan Dogan, Bilgin Taskin

{"title":"Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants.","authors":"Gokhan Dogan, Bilgin Taskin","doi":"10.33073/pjm-2021-026","DOIUrl":"https://doi.org/10.33073/pjm-2021-026","url":null,"abstract":"Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium, and Bacillus genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"297-304"},"PeriodicalIF":2.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/82/pjm-70-3-297.PMC8456377.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39466497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12