{"title":"某些禾本科植物内生细菌产生水解酶的研究。","authors":"Gokhan Dogan, Bilgin Taskin","doi":"10.33073/pjm-2021-026","DOIUrl":null,"url":null,"abstract":"<p><p>Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to <i>Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium</i>, and <i>Bacillus</i> genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":"70 3","pages":"297-304"},"PeriodicalIF":2.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/82/pjm-70-3-297.PMC8456377.pdf","citationCount":"12","resultStr":"{\"title\":\"Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants.\",\"authors\":\"Gokhan Dogan, Bilgin Taskin\",\"doi\":\"10.33073/pjm-2021-026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to <i>Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium</i>, and <i>Bacillus</i> genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.</p>\",\"PeriodicalId\":20272,\"journal\":{\"name\":\"Polish Journal of Microbiology\",\"volume\":\"70 3\",\"pages\":\"297-304\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/82/pjm-70-3-297.PMC8456377.pdf\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2021-026\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/9/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.33073/pjm-2021-026","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/9/17 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants.
Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium, and Bacillus genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.
期刊介绍:
Polish Journal of Microbiology (PJM) publishes original research articles describing various aspects of basic and applied microbiological research. We are especially interested in articles regarding
- basic biological properties of bacteria and archaea, viruses, and simple
- eukaryotic microorganisms
- genetics and molecular biology
- microbial ecology
- medical bacteriology and public health
- food microbiology
- industrial microbiology
- bacterial biotechnology