Polish Journal of Microbiology最新文献

Seroepidemiological Survey of Cytomegalovirus Infection among Pregnant Women in Sudan. 苏丹孕妇巨细胞病毒感染的血清流行病学调查。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-09-20 eCollection Date: 2023-09-01 DOI: 10.33073/pjm-2023-026

Ehssan H Moglad, Ahmed O Hassan, Mawada S Atta Elmanan, Samar M Saeed, Wafaa Mohammed Abdalla, Kawthar Abdelgaleil Mohammedsalih, Hatim T Ali, Mohammed Siddig Abd Elaziz, Hind Haidar Ahmed

{"title":"Seroepidemiological Survey of Cytomegalovirus Infection among Pregnant Women in Sudan.","authors":"Ehssan H Moglad, Ahmed O Hassan, Mawada S Atta Elmanan, Samar M Saeed, Wafaa Mohammed Abdalla, Kawthar Abdelgaleil Mohammedsalih, Hatim T Ali, Mohammed Siddig Abd Elaziz, Hind Haidar Ahmed","doi":"10.33073/pjm-2023-026","DOIUrl":"10.33073/pjm-2023-026","url":null,"abstract":"Human Cytomegalovirus (HCMV) is a leading healthcare problem associated with stillbirth and congenital abnormalities. Determining the seroprevalence and the possible risk factors related to HCMV infections may be a cornerstone in preventing its complications. This cross-sectional study was conducted in Kassala and River Nile States to determine the seroprevalence and risk factors associated with HCMV infection in pregnant women. One hundred eighty-four (n = 184) blood specimens were collected from pregnant women from February 2018 to January 2020. Enzyme-linked immunosorbent assay (ELISA) was used to detect HCMV-specific IgG and IgM antibodies. Socio-demographical characteristics of the women were collected using structured questionnaires. The results showed that HCMV IgG was detected in 170 (92.4%) of the blood specimens, and IgM was detected in 29/93 (31.2%). There was a significant relationship between the history of miscarriage and the presence of IgG and IgM with a p-value = 0.001 and between HCMV IgM and gestational stage (p-value = 0.028). The study found a strikingly high seroprevalence of HCMV infections among pregnant women in the investigated States. This high percentage of illiterate housewives living in rural areas makes it possible to reduce the incidence of HCMV infection in pregnant women by improving their knowledge, attitude, and practice regarding the route of viral transmission, which may reflect in lowering the rate of congenital diseases in their infants.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/71/df/pjm-72-3-pjm-2023-026.PMC10508969.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10157271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Study of Linezolid and Vancomycin Regimens in One-Stage Surgery for Treating Limb Traumatic Osteomyelitis Caused by Methicillin-Resistant Staphylococcus aureus. 利奈唑胺和万古霉素方案一期手术治疗耐甲氧西林金黄色葡萄球菌致四肢外伤性骨髓炎的比较研究。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-09-20 eCollection Date: 2023-09-01 DOI: 10.33073/pjm-2023-024

Rongchang Zhou, Kai Huang, Qiaofeng Guo, Bingyuan Lin, Haiyong Ren, Yiyang Liu, Hongu Song

{"title":"Comparative Study of Linezolid and Vancomycin Regimens in One-Stage Surgery for Treating Limb Traumatic Osteomyelitis Caused by Methicillin-Resistant Staphylococcus aureus.","authors":"Rongchang Zhou, Kai Huang, Qiaofeng Guo, Bingyuan Lin, Haiyong Ren, Yiyang Liu, Hongu Song","doi":"10.33073/pjm-2023-024","DOIUrl":"10.33073/pjm-2023-024","url":null,"abstract":"To compare the clinical outcomes of linezolid and vancomycin regimens combined with one-stage surgery in treating traumatic osteomyelitis of the limbs caused by methicillin-resistant Staphylococcus aureus (MRSA) infection. A retrospective study was performed to analyze patients with traumatic osteomyelitis of the limbs attributable to MRSA infection. All of these patients received one-stage surgery to debride their wounds, with subsequent implantation of a vancomycin-loaded calcium sulfate artificial bone. Patients received either intravenous linezolid (study group) or vancomycin (control group) during the perioperative period. The postoperative inflammatory markers, renal function, duration of drainage catheter placement, duration of antibiotic administration, length of hospital stay, adverse events, and recurrence of osteomyelitis in these two groups were compared. The study group had a shorter duration of antibiotic administration and length of hospital stay (p < 0.05). There was a significant difference in the incidences of adverse events between the two groups (5.88% and 17.65% in the study and control groups, respectively, p < 0.05). There was no recurrence in either group during the three-year follow-up period. As of year, five after the surgery, one patient in the control group had a recurrence of osteomyelitis. The linezolid regimen should be preferred to the vancomycin regimen in patients with traumatic osteomyelitis of the extremities caused by MRSA infection because the linezolid regimen showed fewer adverse events, shorter periods of antibiotic use, and shorter hospital stay. However, both treatment regimens achieved satisfactory outcomes and warranted further investigations.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dd/f6/pjm-72-3-pjm-2023-024.PMC10508968.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10096880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of Biochemical Processes between Chronic Obstructive Pulmonary Disease (COPD), Pulmonary Arterial Hypertension (PAH), and Coronavirus Disease 2019 (COVID-19). 慢性阻塞性肺疾病(COPD)、肺动脉高压(PAH)与2019冠状病毒病(COVID-19)生化过程的相互作用

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-015

Zhe Tian, Lilan Cen

{"title":"Interaction of Biochemical Processes between Chronic Obstructive Pulmonary Disease (COPD), Pulmonary Arterial Hypertension (PAH), and Coronavirus Disease 2019 (COVID-19).","authors":"Zhe Tian, Lilan Cen","doi":"10.33073/pjm-2023-015","DOIUrl":"https://doi.org/10.33073/pjm-2023-015","url":null,"abstract":"Both pulmonary arterial hypertension (PAH) and chronic obstructive pulmonary disease (COPD) are risk factors for coronavirus disease 2019 (COVID-19). Patients with lung injury and altered pulmonary vascular anatomy or function are more susceptible to infections. The purpose of the study is to ascertain whether individuals with COPD or PAH are affected synergistically by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Data sources for the construction of a protein-protein interaction (PPI) network and the identification of differentially expressed genes (DEGs) included three RNA-seq datasets from the GEO database (GSE147507, GSE106986, and GSE15197). Then, relationships between miRNAs, common DEGs, and transcription factor (TF) genes were discovered. Functional analysis using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and other databases, as well as the forecasting of antiviral medications for COPD and PAH patients infected with SARS-CoV-2, were also performed. Eleven common DEGs were found in the three datasets, and their biological functions were primarily enriched in the control of protein modification processes, particularly phosphorylation. Growth factor receptor binding reflects molecular function. KEGG analysis indicated that co-DEGs mainly activate Ras, and PI3K-Akt signaling pathways and act on focal adhesions. NFKB1 interacted with HSA-miR-942 in the TF-miRNA-DEGs synergistic regulatory network. Acetaminophen is considered an effective drug candidate. There are some connections between COPD and PAH and the development of COVID-19. This research could aid in developing COVID-19 vaccines and medication candidates that would work well as COVID-19 therapies.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c9/53/pjm-72-2-pjm-2023-015.PMC10266291.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9628702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Rapid Bactericidal and Anti-Biofilm Alpha-Mangostin In Vitro Activity against Staphylococcus aureus. 快速杀菌和抗生物膜α -山竹苷体外抗金黄色葡萄球菌活性的机制。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-021

Xiangbin Deng, Hongbo Xu, Duoyun Li, Jinlian Chen, Zhijian Yu, Qiwen Deng, Peiyu Li, Jinxin Zheng, Haigang Zhang

{"title":"Mechanisms of Rapid Bactericidal and Anti-Biofilm Alpha-Mangostin In Vitro Activity against Staphylococcus aureus.","authors":"Xiangbin Deng, Hongbo Xu, Duoyun Li, Jinlian Chen, Zhijian Yu, Qiwen Deng, Peiyu Li, Jinxin Zheng, Haigang Zhang","doi":"10.33073/pjm-2023-021","DOIUrl":"https://doi.org/10.33073/pjm-2023-021","url":null,"abstract":"Alpha-mangostin (α-mangostin) was discovered as a potent natural product against Gram-positive bacteria, whereas the underlying molecular mechanisms are still unclear. This study indicated that α-mangostin (at 4 × MIC) rapidly killed Staphylococcus aureus planktonic cells more effectively (at least 2-log10 CFU/ml) than daptomycin, vancomycin and linezolid at 1 and 3 h in the time-killing test. Interestingly, this study also found that a high concentration of α-mangostin (≥4×MIC) significantly reduced established biofilms of S. aureus. There were 58 single nucleotide polymorphisms (SNPs) in α-mangostin nonsensitive S. aureus isolates by whole-genome sequencing, of which 35 SNPs were located on both sides of the sarT gene and 10 SNPs in the sarT gene. A total of 147 proteins with a different abundance were determined by proteomics analysis, of which 91 proteins increased, whereas 56 proteins decreased. The abundance of regulatory proteins SarX and SarZ increased. In contrast, the abundance of SarT and IcaB was significantly reduced (they belonged to SarA family and ica system, associated with the biofilm formation of S. aureus). The abundance of cell membrane proteins VraF and DltC was augmented, but the abundance of cell membrane protein UgtP remarkably decreased. Propidium iodide and DiBaC4(3) staining assay revealed that the fluorescence intensities of DNA and the cell membrane were elevated in the α-mangostin treated S. aureus isolates. In conclusion, this study reveals that α-mangostin was effective against S. aureus planktonic cells by targeting cell membranes. The anti-biofilm effect of α-mangostin may be through inhibiting the function of SarT and IcaB.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/29/pjm-72-2-pjm-2023-021.PMC10266293.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9995043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physicochemical and Microbiological Water Quality Assessment of a Northwestern Algerian Dam: Detection of Ichtyopathogenic Bacteria. 阿尔及利亚西北部一座大坝的理化和微生物水质评价:鱼致病菌的检测。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-020

Hanane Boumerdassi, Lydia Neïla Djouadi, Abderrahmane Hambli, Marie-Laure Fardeau, Hadda-Imen Ouzari, Farida Nateche

{"title":"Physicochemical and Microbiological Water Quality Assessment of a Northwestern Algerian Dam: Detection of Ichtyopathogenic Bacteria.","authors":"Hanane Boumerdassi, Lydia Neïla Djouadi, Abderrahmane Hambli, Marie-Laure Fardeau, Hadda-Imen Ouzari, Farida Nateche","doi":"10.33073/pjm-2023-020","DOIUrl":"https://doi.org/10.33073/pjm-2023-020","url":null,"abstract":"Freshwater fish are often exposed to threats from anthropogenic or natural origins, such as pathogenic or opportunistic microorganisms responsible for a broad range of severe infections. In this study, we aimed to assess this microbiological threat to fish in an Algerian northwestern dam Sekkak (Tlemcen) by evaluating the diversity of ichtyopathogenic bacteria. In order to determine the water quality, physicochemical analyses of the dam water were carried out in situ. Ichtyopathogenic bacteria were isolated on selective media and identified by API galleries and molecular techniques (PCR and sequencing of the 16S rRNA gene). Besides, the antibiograms were constructed for all the isolates. The physicochemical and bacteriological analyses allowed us to classify the dam water as moderately polluted to polluted. Furthermore, an important diversity of ichtyopathogenic bacterial species was observed as Aeromonas hydrophila, Providencia rettgeri, and Pseudomonas aeruginosa were retrieved. The antibiogram test revealed notable resistance. The antibiotic family for which most resistances were found was the β-lactam family, followed by aminoglycosides and macrolides. These results indicate that aquatic environments can shelter multidrug-resistant pathogenic bacteria representing a threat to the endemic fauna. Therefore, it is important to closely monitor these waters in order to improve the fish's living environment and ensure healthier production.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d5/ff/pjm-72-2-pjm-2023-020.PMC10266294.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9995046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Pathogen Isolates in Chronic Wound Infections in Poland. 波兰慢性伤口感染的病原菌分离。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-014

Marcin Malka, Arkadiusz Krakowiecki, Magdalena Chojak, Marek Pławski, Mariusz Wądołek, Agnieszka Wołowicz, Aleksandra Dyczewska, Aleksandra Paź, Katarzyna Pawlik, Tomasz Grzela

{"title":"The Pathogen Isolates in Chronic Wound Infections in Poland.","authors":"Marcin Malka, Arkadiusz Krakowiecki, Magdalena Chojak, Marek Pławski, Mariusz Wądołek, Agnieszka Wołowicz, Aleksandra Dyczewska, Aleksandra Paź, Katarzyna Pawlik, Tomasz Grzela","doi":"10.33073/pjm-2023-014","DOIUrl":"https://doi.org/10.33073/pjm-2023-014","url":null,"abstract":"Chronic wound infection is one of the factors that hinder or prevent its healing. The incidence of infection may vary depending on the type of wound. It is estimated that clinically significant infection in diabetic foot syndrome occurs in up to 30% of patients. Accurate diagnosis of infection features and proper microbiological tests are crucial for introducing of appropriate local and often systemic treatment. The aim of the study was a comparative analysis of the microbiota found in infected chronic wounds in patients from Poland, consulted on an outpatient basis at a wound care center in 2013-2021. The indication for microbiology culture tests was the detection of local signs of infection, and sampling was preceded by appropriate wound debridement. The standard culture technique was a deep-tissue biopsy. Material for the study was collected from 1,199 patients. Overall, 3,917 results of microbiological tests were subjected to retrospective analysis. The paper presents the results in the form of the number of cultured microorganisms and their relative incidence as percentages, considering the division into the types of wounds from which the material was obtained. The most frequently isolated microorganisms in the analyzed group were Staphylococcus aureus (14.3% of this group were MRSA - methicillin-resistant Staphylococcus aureus) and Enterococcus faecalis (2.4% of this group were VRE - vancomycin-resistant Enterococcus). Further analysis of such an extensive database, especially regarding drug susceptibility of isolated microorganisms, seems crucial to elaborate new recommendations for empirical antibacterial treatment of infected chronic wounds.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0e/9f/pjm-72-2-pjm-2023-014.PMC10266287.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9627654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children. 昆明儿童流感嗜血杆菌血清型及分子耐药模式的研究

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-006

Mei Yuan, Mingbiao Ma, Hongchao Jiang, Mao Fan, Ying Sun, Bailing Zhou, Xingxing Feng, Junyi Yang, Min Su, Xiaoli He

{"title":"Characterization of Serotypes and Molecular Drug Resistance Patterns of Haemophilus influenzae in Kunming Children.","authors":"Mei Yuan, Mingbiao Ma, Hongchao Jiang, Mao Fan, Ying Sun, Bailing Zhou, Xingxing Feng, Junyi Yang, Min Su, Xiaoli He","doi":"10.33073/pjm-2023-006","DOIUrl":"https://doi.org/10.33073/pjm-2023-006","url":null,"abstract":"The present study aimed to determine the capsular serotype distribution and antimicrobial drug resistance patterns of Haemophilus influenzae from children in the Kunming region of China. This information could guide policymakers in clinical treatment. In the present study, H. influenzae isolates were tested for their serotypes, antimicrobial susceptibility pattern, and presence of β-lactamases. One-hundred forty-eight H. influenzae strains isolated from children 0-2 years old were investigated for capsular types by glass slide agglutination and molecular methods, and biotyped by the biochemical reactions. The drug resistance-encoding genes TEM-1, ROB-1, and the ftsI gene mutations PBP3-3, and PBP3-BLN were detected with real-time quantitative polymerase chain reaction (qPCR). The prevalence of β-lactamase-producing strains (60.3%) was significantly higher (p < 0.05) than non-enzyme-producing strains. β-Lactamase-producing strains were multidrug resistant to various antibiotics such as ampicillin, tetracycline, sulfamethoxazole/trimethoprim, chloramphenicol, cefuroxime, and cefaclor. Among β-lactamase-producing strains, the detection rates of the TEM-1, PBP3-BLN, PBP3-s, and ROB-1 were 54.1%, 18.9%, 11.8%, and 6.9%, respectively. The biotyping results show that most H. influenzae strains were of type II and III. Non-typeable H. influenzae (NTHi) accounted for 89.3% of the strains. NTHi strains were the most prevalent in this region; most belonged to biological types II and III. β-Lactamase-positive ampi-cillin-resistant (BLPAR) strains were prevalent among H. influenzae isolates in this region.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/de/eb/pjm-72-2-pjm-2023-006.PMC10266285.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9992899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Resistance and Biofilm Formation of Staphylococcus aureus Isolates from Febrile Cases: Findings from a Rural Cohort of Odisha, India. 从发热病例分离的金黄色葡萄球菌的抗菌素耐药性和生物膜形成:来自印度奥里萨邦农村队列的发现。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-005

Pujarini Dash, Khokan Rana, Jyotirmayee Turuk, Subrata Kumar Palo, Sanghamitra Pati

引用次数: 0

Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa). 南非理查兹湾堆肥样品中潜在木质素过氧化物酶产菌的分离与鉴定

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-003

Sindiswa L Dube, Foluso O Osunsanmi, Bongekile P Ngcobo, Londiwe B Mkhwanazi, Zanele Z Jobe, Raphael T Aruleba, Rebamang A Mosa, Andrew R Opoku

{"title":"Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa).","authors":"Sindiswa L Dube, Foluso O Osunsanmi, Bongekile P Ngcobo, Londiwe B Mkhwanazi, Zanele Z Jobe, Raphael T Aruleba, Rebamang A Mosa, Andrew R Opoku","doi":"10.33073/pjm-2023-003","DOIUrl":"https://doi.org/10.33073/pjm-2023-003","url":null,"abstract":"Lignin recalcitrance is a key issue in producing value-added products from lignocellulose biomass. In situ biodegradable lignin-modifying enzymes-producing bacteria are considered a suitable solution to lignin biodegradation problems, but exploitation of ligninolytic bacteria is still limited to date. Hence, this study aimed to isolate and characterize potential lignin peroxidase ligninolytic bacteria from decomposing soil, sawdust, and cow dung at Richard Bay, South Africa. The samples were collected and cultured in the lignin-enriched medium. Pure isolated colonies were characterized through 16S rRNA gene sequencing. The ability of the isolates to grow and utilize aromatic monomers (veratryl and guaiacol alcohol) and decolorize lignin-like dyes (Azure B, Congo Red, Remazol Brilliant Blue R) was evaluated. Of the twenty-six (26) bacteria isolates 10 isolates, including Pseudomonas spp. (88%), Enterobacter spp. (8%), and Escherichia coli (4%) were identified as true lignin peroxidase producers. Pseudomonas aeruginosa (CP031449.2) and E. coli (LR025096.1) exhibited the highest ligninolytic activities. These isolates could potentially be exploited in the industry and wastewater treatment as effective lignin degrading agents.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/85/pjm-72-2-pjm-2023-003.PMC10266290.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9993478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stalactites Core Prospect as Environmental "Microbial Ark": The Actinomycetota Diversity Paradigm, First Reported from a Greek Cave. 钟乳石岩心作为环境“微生物方舟”的前景:首次在希腊洞穴中报道的放线菌群多样性范式。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-06-01 DOI: 10.33073/pjm-2023-016

Ioannis Vagelas, Angeliki Reizopoulou, Athanasios Exadactylos, Panagiotis Madesis, Lefkothea Karapetsi, George Michail

引用次数: 0