Plant Biotechnology Reports最新文献_第5页

Regulation of coconut somatic embryogenesis: decoding the role of long non-coding RNAs 椰子体细胞胚胎发生的调控：解码长非编码 RNA 的作用

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-01-19 DOI: 10.1007/s11816-023-00884-z

A. A. Sabana, Ginny Antony, K. P. Gangaraj, Tony Grace, M. K. Rajesh

{"title":"Regulation of coconut somatic embryogenesis: decoding the role of long non-coding RNAs","authors":"A. A. Sabana, Ginny Antony, K. P. Gangaraj, Tony Grace, M. K. Rajesh","doi":"10.1007/s11816-023-00884-z","DOIUrl":"https://doi.org/10.1007/s11816-023-00884-z","url":null,"abstract":"Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides that lack significant protein coding potential and have been shown to regulate various biological processes. This study was designed to identify lncRNAs in coconut and their role in the process of somatic embryogenesis in coconut, a crop with high recalcitrance to in vitro culture. RNA-Seq data of coconut embryogenic calli of the West Coast Tall cultivar was exploited for in silico prediction of lncRNA. From a total of 6328 transcripts, which were annotated as uncharacterised or with no homology hits with the existing database, 5110 putative lncRNAs are identified. We also studied the relationship between lncRNAs, microRNAs (miRNAs) and mRNAs and found that some of the lncRNAs act as miRNA precursors, some as potential miRNA targets and some function as endogenous target mimics (eTMs) for miRNAs. Real-time quantitative PCR confirmed that 10 selected lncRNAs showed significant differences in the expression pattern in different stages of coconut somatic embryogenesis. Our results suggest the existence of diverse lncRNAs in coconut embryogenic calli, some of which are differentially expressed. The information generated in this study could be of great value in understanding the molecular mechanisms governing somatic embryogenesis in coconut.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139507277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and functional analysis of the PtEXLA1 gene from poplar 杨树 PtEXLA1 基因的特征和功能分析

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-01-06 DOI: 10.1007/s11816-023-00885-y

引用次数: 0

Genome-wide identification of wheat ABC gene family and expression in response to fungal stress treatment 小麦 ABC 基因家族的全基因组鉴定及对真菌胁迫处理的响应表达

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-12-29 DOI: 10.1007/s11816-023-00881-2

Guanghao Wang, Jianhua Gu, Deyu Long, Xiangyu Zhang, Chenxu Zhao, Hong Zhang, Chunhuan Chen, Wanquan Ji

{"title":"Genome-wide identification of wheat ABC gene family and expression in response to fungal stress treatment","authors":"Guanghao Wang, Jianhua Gu, Deyu Long, Xiangyu Zhang, Chenxu Zhao, Hong Zhang, Chunhuan Chen, Wanquan Ji","doi":"10.1007/s11816-023-00881-2","DOIUrl":"https://doi.org/10.1007/s11816-023-00881-2","url":null,"abstract":"The ATP-binding cassette (ABC) transporter family is one of the largest protein families in plants and plays an essential role in addressing biotic and abiotic stresses. Wheat, a vital global grain crop, faces multifaceted safety challenges, primarily from fungal diseases like stripe rust and powdery mildew. In the present study, we identified the whole genome of the wheat ABC family, and 463 nonredundant ABC genes were identified. The ABC family can be divided into nine evolutionary branches and eight subfamilies based on phylogenetic tree analysis. This paper delved deeper into characterizing the gene structure, promoter region, and gene expression within the TaABC family. Segmental duplication was the main reason for the expansion of the TaABC genes. Ka/Ks analysis suggested that most TaABC genes were intensely purified and selected. The collinear analysis of TaABC and other species showed that the ABC genes were conserved in evolution. RNA-seq data and qPCR data from wheat infected with powdery mildew or stripe rust showed that most TaABC genes were induced to change expression. The candidate genes TaABCB15-3B and TaABCG38 exhibited responsiveness to powdery mildew in resistant/susceptible wheat, while remaining unresponsive to stripe rust. Our findings serve as a valuable reference for gaining a deeper understanding of the function and evolution of TaABCs, aiding in the identification of enduring disease resistance genes within the TaABCs of wheat.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetics: Toward improving crop disease resistance and agronomic characteristics 表观遗传学：提高作物抗病性和农艺特性

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-12-13 DOI: 10.1007/s11816-023-00876-z

Chibuzo Sampson, Tuzymeshach Holyword Ikenwugwu, Innocent Uzochukwu Okagu, Ibrahim Inuwa Yahaya, Chuks Kenneth Odoh, Chibuzor Nwadibe Eze

引用次数: 0

HB31 and HB21 regulate floral architecture through miRNA396/GRF modules in Arabidopsis 拟南芥中的HB31和HB21通过miRNA396/GRF模块调控花卉结构

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-12-12 DOI: 10.1007/s11816-023-00870-5

Young Koung Lee, Andrew Olson, Keunhwa Kim, Masaru Ohme-Takagi, Doreen Ware

{"title":"HB31 and HB21 regulate floral architecture through miRNA396/GRF modules in Arabidopsis","authors":"Young Koung Lee, Andrew Olson, Keunhwa Kim, Masaru Ohme-Takagi, Doreen Ware","doi":"10.1007/s11816-023-00870-5","DOIUrl":"https://doi.org/10.1007/s11816-023-00870-5","url":null,"abstract":"Floral architecture plays a pivotal role in developmental processes under genetic regulation and is also influenced by environmental cues. This affects the plant silique phenotype in Arabidopsis and grain yield in crops. Despite the relatively small number of family members of zinc finger homeodomain (ZF-HD) transcription factors (TFs) in plants, their biological role needs to be investigated to understand the molecular mechanisms associated with plant developmental processes. Therefore, we generated HB31SRDX and HB21SRDX repressor mutant lines to understand the functional role of ZF-HD TFs. The mutant lines showed severe defects in plant architecture, including increased branching number, reduced plant height, distorted floral phenotype, and short silique. We found that HB31 and HB21 are paralogs in Arabidopsis, and both positively regulate cell size-related genes, cell wall modification factor-related genes, and M-type MADS-box TF families. In addition, HB31 and HB21 are negatively associated with abiotic stress-related genes, vegetative-to-reproductive phase transition of meristem-related genes, and TCP and RAV TFs. microRNA164 (miR164), miR822, miR396, miR2934, and miR172 were downregulated, whereas miR169, miR398, miR399, and miR157 were upregulated in the two repressor lines. Phenotypic and molecular analyses demonstrated that the miR396/GRF modules regulated by HB31 and HB21 are involved in the plan floral architecture of Arabidopsis. The findings of this study will help elucidate the role of ZF-HD TFs in maintaining the floral architecture.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138573632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing crop health and sustainability: exploring the potential of secondary metabolites and non-thermal plasma treatment as alternatives to pesticides 增强作物健康和可持续性：探索次级代谢物和非热等离子体处理作为杀虫剂替代品的潜力

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-12-08 DOI: 10.1007/s11816-023-00883-0

Himani Singh, Niharika, Pradeep Lamichhane, Ravi Gupta, Neha Kaushik, Eun Ha Choi, Nagendra Kumar Kaushik

{"title":"Enhancing crop health and sustainability: exploring the potential of secondary metabolites and non-thermal plasma treatment as alternatives to pesticides","authors":"Himani Singh, Niharika, Pradeep Lamichhane, Ravi Gupta, Neha Kaushik, Eun Ha Choi, Nagendra Kumar Kaushik","doi":"10.1007/s11816-023-00883-0","DOIUrl":"https://doi.org/10.1007/s11816-023-00883-0","url":null,"abstract":"Pesticides have been an integral part of modern agriculture as their use ensures good harvests. However, excessive use of pesticides in the last few decades has caused significant environmental degradation. Moreover, excessive use of pesticides causes stress on crops and non-target plants and exhibits toxicity to other organisms including mammals, microbes, and insects. Plants employ various morphological, physiological, and biochemical mechanisms to reduce pesticides toxicity. One such mechanism is production of secondary metabolites that improves stress tolerance of plants. In addition, recent studies have also highlighted a potential role of plasma technology in mitigating various abiotic and biotic environmental stresses. Besides, plasma treatment improves seed germination, physiological processes, and seedling establishment during the early growth stages of a plant under adverse and non-adverse conditions and thus can be used an alternate to the pesticide treatment. This review article summarizes recent advancements in understanding the synthesis, accumulation, and transportation of secondary metabolites which have significant relevance to crop improvement programs. We also present an overview of the effects of plasma treatment on phytopathogenic bacterial cell suspensions and plant responses to metabolic activity. In the future, researchers need to develop innovative ideas to reduce the use of chemical pesticides in farming practices.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138560509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular identity for commercially important inter-specific hybrids of Coffea using ISSR-DNA marker: implication on genetic improvement 利用ISSR-DNA标记对具有商业价值的咖啡种间杂交种进行分子鉴定:对遗传改良的启示

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-12-01 DOI: 10.1007/s11816-023-00878-x

Sreedevi Amruthakumar, Bhavatharani Manivel, Karthiga Sivamani, Thilaga Sethuraman, Nyani Surya Prakash Rao, Doss Ganesh

{"title":"Molecular identity for commercially important inter-specific hybrids of Coffea using ISSR-DNA marker: implication on genetic improvement","authors":"Sreedevi Amruthakumar, Bhavatharani Manivel, Karthiga Sivamani, Thilaga Sethuraman, Nyani Surya Prakash Rao, Doss Ganesh","doi":"10.1007/s11816-023-00878-x","DOIUrl":"https://doi.org/10.1007/s11816-023-00878-x","url":null,"abstract":"Six popular and widely cultivated arabica coffee (Coffea arabica L.) varieties of commercial importance namely Selection 5B, Selection 13, Selection 11, Selection 8, Selection 7.3 and Selection 3 were tested for their genetic identity with ISSR markers. Fifteen ISSR primers were tested using genomic DNA of selected coffee varieties. Pooled genomic DNA of all the six varieties was amplified with each ISSR primer with an average of four loci per primer. The size range of locus amplified by all the fifteen primers was ranging from 100 to 1200 bp depending upon on the ISSR primers. Only three out of fifteen primers, namely ISSR4, ISSR6 and ISSR8, were screened based on the number of amplified locus and size range from low to high. The selective ISSR primers distinguished all the six varieties of coffee with unique markers. ISSR 4 amplified two unique markers with a locus size 1300 bp and 950 bp for Sln.5B and 180 bp and 150 bp for Sln.13. ISSR6 had produced five varietal-specific markers with a locus size of 180 bp in Sln.5B, 1250 bp in Sln.11, 350 bp in Sln.3. ISSR8 had amplified seven unique loci across the coffee varieties with 700 bp and 800 bp in Sln.5B, 200 bp and 500 bp in Sln.11 and one locus each in Sln.7.3 and Sln.3 with 300 bp and 150 bp respectively. Repeated amplification of genomic DNA of all the six varieties of coffee with selective ISSR primers produced consistent ISSR genetic fingerprints. Selective ISSR primers were validated with marker parameters resolving power (RP), effective multiplex ratio (EMR), marker index (MI) and polymorphic information content (PIC). Utilisation of these markers in arabica coffee genetic improvement is discussed.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific binding of plant-expressed anti-PD-L1 monoclonal antibody to multiple myeloma cell line RPMI8226 植物表达的抗 PD-L1 单克隆抗体与多发性骨髓瘤细胞系 RPMI8226 的特异性结合

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-30 DOI: 10.1007/s11816-023-00882-1

Caiquan Jin, Chae-Eun Lee, Hyunjoo Hwang, Yerin Kim, Peter Hinterdorfer, Soon Chul Myung, Sungsu Park, Mi Kyung Kim, Mineui Hong, Kisung Ko

{"title":"Specific binding of plant-expressed anti-PD-L1 monoclonal antibody to multiple myeloma cell line RPMI8226","authors":"Caiquan Jin, Chae-Eun Lee, Hyunjoo Hwang, Yerin Kim, Peter Hinterdorfer, Soon Chul Myung, Sungsu Park, Mi Kyung Kim, Mineui Hong, Kisung Ko","doi":"10.1007/s11816-023-00882-1","DOIUrl":"https://doi.org/10.1007/s11816-023-00882-1","url":null,"abstract":"Multiple myeloma (MM) is an incurable disease characterized by malignant plasma cells within the bone marrow, and its increasing occurrence has highlighted the need for innovative strategies to address relapse and treatment resistance. Given the substantial expression of programmed death ligand 1 (PD-L1) in the human multiple myeloma cell line RPMI8226, we propose PD-L1 as a promising target for multiple myeloma therapy. Here, we successfully engineered an anti-PD-L1 monoclonal antibody (mAb) within a plant-based system. Building upon our previous findings, we germinated seeds derived from transgenic plants under in vitro conditions. Afterward, we screened the resulting seedlings for expression of the anti-PD-L1 mAb using polymerase chain reaction (PCR) and western blot analyses. Anti-PD-L1 mAbs were successfully purified from plant leaves and characterized through SDS-PAGE analysis. Our findings, which were confirmed via indirect enzyme-linked immunosorbent assay (ELISA), validate the binding affinity of the anti-PD-L1 mAb to recombinant PD-L1 protein. Furthermore, we investigated the interaction between the plant-derived anti-PD-L1 mAb and Fc gamma receptor I (FcγRI) as well as Fc gamma receptor IIIa (FcγRIIIa) molecules, confirming robust affinity. Additionally, the antibody’s binding affinity to the human multiple myeloma cancer cell line RPMI8226 was confirmed via cell ELISA. Our findings demonstrated that, unlike existing therapeutics, the plant-derived anti-PD-L1 antibody not only effectively binds to human recombinant PD-L1 protein but also to FcγRI and FcγRIIIa. These findings suggest the potential of plant-derived anti-PD-L1 mAb for the development of innovative therapies against multiple myeloma, emphasizing the need for further research and preclinical evaluation.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138580468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of loop-mediated isothermal amplification technique for LM canola T45, 73496, and MON88302 and its field application LM油菜T45、73496和MON88302环介导等温扩增技术的建立及其田间应用

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-29 DOI: 10.1007/s11816-023-00879-w

Wonkyun Choi, A-Mi Yoon, Hye Song Lim, Jung Ro Lee

{"title":"Development of loop-mediated isothermal amplification technique for LM canola T45, 73496, and MON88302 and its field application","authors":"Wonkyun Choi, A-Mi Yoon, Hye Song Lim, Jung Ro Lee","doi":"10.1007/s11816-023-00879-w","DOIUrl":"https://doi.org/10.1007/s11816-023-00879-w","url":null,"abstract":"Brassica is an essential genus in agriculture, and gene flow from living modified (LM) organisms to native relatives or non-modified cultivars of the crucifer family is a critical issue in Asia. Loop-mediated isothermal amplification (LAMP) is a simple, rapid, and accurate method for identifying LM crops. This study aimed to develop a LAMP assay for the laboratory diagnosis and field analysis of three novel LM canola events: T45, 73496, and MON88302. The genomic DNA of each LM canola was amplified by incubating at 63 °C for 30 min with a newly developed 2 × LAMP premix containing Bst DNA polymerase and event-specific primer sets. The sensitivity of the LAMP assay for the three canola events in our condition ranged from 100 pg·μL−1 to 1 ng·μL−1. Genetic elements of the three LM events were identified through a combination of the LAMP assay, a syringe-type DNA extraction kit, and a portable isothermal amplifier. This study proposes a novel, simple system based on isothermal amplification for the DNA detection of the three LM canola events in a survey field.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of photosynthesis in changing environment: approaches, achievements and prospects 变化环境下光合作用的改善:途径、成就与展望

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-28 DOI: 10.1007/s11816-023-00871-4

Sultan Suboktagin, Ghazal Khurshid, Misbah Bilal, Anum Zeb Abbassi, Suk-Yoon Kwon, Raza Ahmad

{"title":"Improvement of photosynthesis in changing environment: approaches, achievements and prospects","authors":"Sultan Suboktagin, Ghazal Khurshid, Misbah Bilal, Anum Zeb Abbassi, Suk-Yoon Kwon, Raza Ahmad","doi":"10.1007/s11816-023-00871-4","DOIUrl":"https://doi.org/10.1007/s11816-023-00871-4","url":null,"abstract":"Photosynthesis is responsible for sustained plant productivity and ensures food supply. The change in global climatic patterns affects photosynthesis that subsequently reduces plant yield and poses threat to food security. Photosynthesis relies on a dual nature enzyme ribulose 1, 5 bisphosphate carboxylase oxygenase (Rubisco), which can fix CO2 as well as O2. The fixation rate of CO2 to O2 depends upon the relative concentration of CO2 inside chloroplast. Higher level of CO2 results in improved photosynthesis, however, its concentration depends upon environmental conditions. Under adverse climate conditions, the CO2 level drops down that leads to increased oxygenation which impedes the photosynthesis and reduces plant productivity. The impact is more significant and apparent specifically in C3 plants. Attempts have been made to address the loss in photosynthesis and multiple strategies have been adapted to date that focus on improvement of photosynthesis in C3 plants. In this review, we have discussed the multiple strategies being employed by different researchers to date for improvement of photosynthesis. The strategies discussed in this review include: improving the performance of Rubisco, engineering CO2-concentrating mechanism of C4 photosynthesis into C3 species, transformation of bicarbonate transporters from cyanobacteria into chloroplasts of C3 plants, and establishment of photorespiratory bypasses to catabolise toxic glycolate in shortest possible pathway.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0