Plant Biotechnology Reports最新文献_第6页

Safety management regulation and practice standards on living modified organism (LMO) facilities under the Ministry of Environment 环境部《改性活生物体(LMO)设施安全管理规定和操作标准》

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-24 DOI: 10.1007/s11816-023-00872-3

Kyong-Hee Nam, Jung Ro Lee

{"title":"Safety management regulation and practice standards on living modified organism (LMO) facilities under the Ministry of Environment","authors":"Kyong-Hee Nam, Jung Ro Lee","doi":"10.1007/s11816-023-00872-3","DOIUrl":"https://doi.org/10.1007/s11816-023-00872-3","url":null,"abstract":"Living modified organisms (LMOs) in South Korea are managed under the Transboundary Movement, Etc. of LMOs Act, the domestic implementation law of the Cartagena Protocol on Biosafety. The Ministry of Environment (MOE) oversees the risk review and safety management of LMOs for environmental remediation, and consultative review on the risk posed to the natural ecosystem by LMOs for other purposes. In particular, the MOE operates a risk assessment institute to promote scientific and systematic safety management by standardizing an evaluation criterion for the relevant LMOs. These must be established according to the standards of the LMOs Act. In this review, a new technique for reliable management of the LMO facility, including a confined field, was proposed to comply with the safety standards of the MOE. Based on the analysis of the LMOs Act and the practices of established LMO facilities, potential facility management directions under the MOE were discussed from the perspectives of the facility, cultivation, waste, record, and education. These suggestions can help physically isolate LMO facilities and prevent the unintentional release of LMOs, thus helping avoid hybridization between LMOs and non-LMOs. In addition, a monitoring system was proposed to prevent transgene escape and subsequent hybridization with the wild relatives of LMOs. These proposals can be widely used for safety management when researching the development and commercialization of LMOs at facilities under the MOE.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"46 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis of Lamiaceae based on transcriptome data 基于转录组数据的紫唇科植物系统发育分析

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-23 DOI: 10.1007/s11816-023-00869-y

Hyewon Kim, Yuna Kang, Wonkyun Joo, Changsoo Kim

{"title":"Phylogenetic analysis of Lamiaceae based on transcriptome data","authors":"Hyewon Kim, Yuna Kang, Wonkyun Joo, Changsoo Kim","doi":"10.1007/s11816-023-00869-y","DOIUrl":"https://doi.org/10.1007/s11816-023-00869-y","url":null,"abstract":"The Lamiaceae family is included in the angiosperms and comprises over 7000 species, many of which are of considerable ecological, economic, and cultural importance. We seek to establish a taxonomic basis by examining the speciation timeline in Lamiaceae using phylogenetics and publicly available transcriptome data. Since Ks is steadily accumulated over time in plants for environmental adaptation until speciation occurs, the timing of speciation can be estimated from examination of Ks values. A total of 24 species included in the Lamiaceae family used in our analysis belongs to four subfamilies. We performed transcriptome assembly for each of the 24 species using trimmed data collected from public databases. We compiled groups of gene families in which at least one copy of the gene is present in each species from orthologous groups among unigenes. From these groups, we obtained a total of 450,014 single nucleotide polymorphisms (SNPs) across 27 species, incorporating three additional outgroup species. Subsequently, a tree was created using these SNPs. In our tree, the outgroup species were clearly located externally, confirming the proximity of species within the same subfamily. The Ks peak corroborated the outcomes observed in the phylogenetic tree. We estimated the rate of sequence evolution and divergence time for each species on the phylogenetic tree by referencing the time of divergence among the Lamiaceae family. In particular, Clinopodium serpyllifolium, Lavandula × intermedia, Phlomis fruticosa, and Volkameria inermis were analyzed for the first time. Our study helps with the understanding of the function of plants included in the Lamiaceae family and is expected to provide a fundamental resource that can be used to pinpoint the molecular and genomic evolution of the Lamiaceae family.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"24 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of storage protein gene using CRISPR/Cas9 removed α′-subunit of β-conglycinin in soybean seeds 利用CRISPR/Cas9基因突变大豆种子中储存蛋白基因，去除β-甘氨酸的α′-亚基

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-22 DOI: 10.1007/s11816-023-00880-3

Da Hyeon Ha, Hye Jeong Kim

引用次数: 0

PnCHS1, a chalcone synthase from the Antarctic moss Pohlia nutans, improves the tolerance of salt stress and ABA 来自南极苔藓的查尔酮合成酶PnCHS1提高了对盐胁迫和ABA的耐受性

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-18 DOI: 10.1007/s11816-023-00873-2

Chaochao Li, Xinhui Xu, Shenghao Liu, Pengying Zhang

{"title":"PnCHS1, a chalcone synthase from the Antarctic moss Pohlia nutans, improves the tolerance of salt stress and ABA","authors":"Chaochao Li, Xinhui Xu, Shenghao Liu, Pengying Zhang","doi":"10.1007/s11816-023-00873-2","DOIUrl":"https://doi.org/10.1007/s11816-023-00873-2","url":null,"abstract":"Chalcone synthase (CHS), a key enzyme in plant flavonoid synthesis, is essential for plant tolerance to abiotic stress. However, little research on CHS from the earliest terrestrial plants, such as mosses, has been reported. Here, the biological function of a CHS gene from Antarctic moss Pohlia nutans (PnCHS1) was studied. PnCHS1 had a 32.8–53.7% similarity to CHS from other species, however it still had highly conserved motifs of CHS such as Catalytic site (Asn366, His333) and Co-A binding site (Ser146). Subcellular localization analysis showed that PnCHS1 was distributed in the cell membrane and in the membranes of endothelial organelles. Heterologous expression of PnCHS1 increased flavonoid content in 5-day-old Arabidopsis grown with 24 h light and 17-day-old Arabidopsis cultured with sucrose, as well as anthocyanin content in the latter. PnCHS1 heterologous expression in Arabidopsis increased plant tolerance to salt stress, including a high germination rate and a long taproot. Heterologous expression of PnCHS1 boosted tolerance to oxidative stress while decreasing the sensitivity to ABA. Under H2O2 or ABA stress, the expression pattern of PnCHS1, ROS scavenging enzyme gene (FeSOD1, FeSOD2, Cu-Zn-SOD2, and Cu-Zn-SOD3) and three genes of ABA signal pathway (RAB18, RD29B, and NCED3) were considerably up-regulated by real-time quantitative analysis. It indicates that PnCHS1 could enhance plant tolerance to NaCl and oxidative stresses, and may play a role in the adaptation of Antarctic moss to extreme environments.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"21 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of transcriptomic profiles and physiological traits of exogenous 24-epibrassinolide alleviating salt stress in Atractylodes macrocephala Koidz. 外源24-表油菜素内酯缓解白术盐胁迫的转录组学特征及生理特性分析。

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-15 DOI: 10.1007/s11816-023-00874-1

Jianyun Zhang, Huiyong Fang, Jianming Jiang, Xian Gu, Huimei Zhao, Jinmiao Qiu, Qian Wang, Yuguang Zheng, Kaiyan Zheng

{"title":"Analysis of transcriptomic profiles and physiological traits of exogenous 24-epibrassinolide alleviating salt stress in Atractylodes macrocephala Koidz.","authors":"Jianyun Zhang, Huiyong Fang, Jianming Jiang, Xian Gu, Huimei Zhao, Jinmiao Qiu, Qian Wang, Yuguang Zheng, Kaiyan Zheng","doi":"10.1007/s11816-023-00874-1","DOIUrl":"https://doi.org/10.1007/s11816-023-00874-1","url":null,"abstract":"Exogenous 24-epibrassinolide (24-epiBL) application is a preferred method for improving salt tolerance in plants. However, information about the effects of exogenous 24-epiBL application related to salt response in Atractylodes macrocephala Koidz. (AMK) has rarely been reported. Here, we aimed to investigate the effects of exogenous 24-epiBL (0.01 mg L−1) on enhancing salt tolerance in AMK seedlings under salt stress (5 g L−1 NaCl) based on seed germination experiments. Our results indicated that NaCl treatment suppresses AMK seed germination, while 24-epiBL treatment under nonstress and stress conditions had positive effects on plant seedling growth. Treatment with NaCl significantly inhibited radicle elongation and root activity to endure salt stress, and 24-epiBL treatment promoted growth and alleviated the damage to osmotic stress, ion stress substances, and oxidative stress by regulating free proline (Pro) and soluble protein (SP) contents and enhancing soluble sugar (SS) conversion. Moreover, the expression of normal development process genes was affected, and differentially expressed genes (DEGs) in multiple pathways associated with amino acid metabolism, signal transduction, carbohydrate metabolism, and lipid metabolism were regulated under NaCl treatment. Further analysis of the AMK transcriptome profile revealed that exogenous 24-epiBL enhanced salt tolerance through hormone regulation and carbohydrate metabolism promotion, salt-responsive gene expression inhibition, and induction of pathways related to terpenoid and polyketide metabolism as well as pentose and glucuronate interconversions for plant growth promotion. Collectively, our findings successfully demonstrate that exogenous 24-epiBL plays a positive role in regulating the AMK response to salt stress, which provides potential genetic resources to improve salt tolerance in plants.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"22 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression analysis of sweet potato patatin-like protein (PLP) genes in response to infection with the root knot nematode Meloidogyne incognita 甘薯斑块样蛋白(PLP)基因对根结线虫侵染的表达分析

4区生物学

Plant Biotechnology Reports Pub Date : 2023-11-07 DOI: 10.1007/s11816-023-00868-z

Jaewook Kim, Jung-Wook Yang, Donghwan Shim, Yun-Hee Kim

引用次数: 0

Isolation and characterization of high-CO2 sensitive Nannochloropsis salina mutant 高co2敏感性纳米盐绿藻突变体的分离与鉴定

4区生物学

Plant Biotechnology Reports Pub Date : 2023-10-16 DOI: 10.1007/s11816-023-00866-1

Jong-Min Lim, Sokyong Jung, Sung Ran Min, Won-Joong Jeong

引用次数: 0

Acknowledgment 鸣谢

4区生物学

Plant Biotechnology Reports Pub Date : 2023-10-12 DOI: 10.1007/s11816-023-00867-0

引用次数: 0

The complete chloroplast genome of two Syzygium (Myrtaceae) species and comparative analysis with other related species 两种桃金娘属植物叶绿体全基因组及其与其它近缘种的比较分析

4区生物学

Plant Biotechnology Reports Pub Date : 2023-10-11 DOI: 10.1007/s11816-023-00865-2

Mengjie Zhang, Xiuyao Yang, Ling Zhu, Dengxian Xi, Hanbing Cai, Tuo Yin, Hanyao Zhang

引用次数: 0

Efficient plantlet regeneration via somatic embryogenesis and shoot organogenesis in Dichanthium annulatum (Forssk.): a halophyte C4 apomictic forage crop 盐生C4无生殖草料作物环双硫磷体细胞胚发生和茎器官发生的高效植株再生

4区生物学

Plant Biotechnology Reports Pub Date : 2023-09-28 DOI: 10.1007/s11816-023-00864-3

None Shashi, Pooja Jha Maity, Vishnu Bhat

引用次数: 0