{"title":"利用CRISPR/Cas9基因突变大豆种子中储存蛋白基因，去除β-甘氨酸的α′-亚基","authors":"Da Hyeon Ha, Hye Jeong Kim","doi":"10.1007/s11816-023-00880-3","DOIUrl":null,"url":null,"abstract":"<p>β-Conglycinin (7S) and glycinin (11S) are the two major storage proteins in soybean seeds. Storage proteins occupy a large portion of soybean seeds, which is the main obstacle to accumulating additional foreign proteins. Thus, we produced 7S-edited soybean plants using CRISPR/Cas9 via <i>Agrobacterium</i>-mediated transformation. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis confirmed that the α′-subunit (76 kDa) of β-conglycinin protein disappeared from the 7S-edited T<sub>2</sub> line (#19–8). The removal of the band was caused by the early termination of the <i>CG-1</i> gene by insertion/deletion (in/del) mutations with an efficiency of 78.5% generated by genome editing. Soybeans with low-storage proteins could be used for improved foreign protein production in seed.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mutation of storage protein gene using CRISPR/Cas9 removed α′-subunit of β-conglycinin in soybean seeds\",\"authors\":\"Da Hyeon Ha, Hye Jeong Kim\",\"doi\":\"10.1007/s11816-023-00880-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>β-Conglycinin (7S) and glycinin (11S) are the two major storage proteins in soybean seeds. Storage proteins occupy a large portion of soybean seeds, which is the main obstacle to accumulating additional foreign proteins. Thus, we produced 7S-edited soybean plants using CRISPR/Cas9 via <i>Agrobacterium</i>-mediated transformation. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis confirmed that the α′-subunit (76 kDa) of β-conglycinin protein disappeared from the 7S-edited T<sub>2</sub> line (#19–8). The removal of the band was caused by the early termination of the <i>CG-1</i> gene by insertion/deletion (in/del) mutations with an efficiency of 78.5% generated by genome editing. Soybeans with low-storage proteins could be used for improved foreign protein production in seed.</p>\",\"PeriodicalId\":20216,\"journal\":{\"name\":\"Plant Biotechnology Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-11-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biotechnology Reports\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s11816-023-00880-3\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology Reports","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11816-023-00880-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Mutation of storage protein gene using CRISPR/Cas9 removed α′-subunit of β-conglycinin in soybean seeds
β-Conglycinin (7S) and glycinin (11S) are the two major storage proteins in soybean seeds. Storage proteins occupy a large portion of soybean seeds, which is the main obstacle to accumulating additional foreign proteins. Thus, we produced 7S-edited soybean plants using CRISPR/Cas9 via Agrobacterium-mediated transformation. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis confirmed that the α′-subunit (76 kDa) of β-conglycinin protein disappeared from the 7S-edited T2 line (#19–8). The removal of the band was caused by the early termination of the CG-1 gene by insertion/deletion (in/del) mutations with an efficiency of 78.5% generated by genome editing. Soybeans with low-storage proteins could be used for improved foreign protein production in seed.
期刊介绍：
Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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