板栗五环三萜合成中氧化角鲨烯环化酶的研究

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jung Yeon Han, Chang-Ho Ahn, Han Suk Choi, Yong Eui Choi
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引用次数: 0

摘要

植物三萜是一种具有丰富化学多样性和有趣生物学特性的次生代谢产物。栗子是一种落叶乔木,栗子是一种重要的食物。在本研究中,我们鉴定了板栗(Castanea crenata)叶和(或)茎皮中不同类型的三萜,如α-amyrin、β-amyrin、lupel和friedelin。三萜的生物合成是由氧化角鲨烯环化酶(OSCs)催化2,3-氧化角鲨烯环化成三萜。利用PacBio测序技术,从金针桃叶片转录组数据中获得了65个推测的OSC序列。我们选择了5个假定的OSC单基因,命名为CcOSC1-5,用于三萜生物合成相关基因的功能表征。通过在erg7突变体酵母中异源表达CcOSC1-5基因的功能表征表明,CcOSC1和CcOSC2具有相似的功能,编码多功能三萜合成酶,主要产生β-amyrin和少量α-amyrin和lupoil。CcOSC3编码混合amyrin合成酶,主要产生β-amyrin和少量α-amyrin。CcOSC4主要产生α-amyrin和lupeol,少量产生β-amyrin。CcOSC5编码一种酶,用于作为单一产物生产lup酮。综上所述,我们鉴定出了crenata中参与β-amyrin、α-amyrin和lupel生物合成的多种三萜,并对三萜合成酶基因进行了功能表征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterization of oxidosqualene cyclases involved in pentacyclic triterpene biosynthesis in Korean chestnut (Castanea crenata)

Characterization of oxidosqualene cyclases involved in pentacyclic triterpene biosynthesis in Korean chestnut (Castanea crenata)

Plant triterpenoids are secondary metabolites with high chemical diversity and interesting biological properties. Chestnuts are deciduous trees in the genus Castanea, and their nuts have been used as an important food. In this work, we identified various types of triterpenes, such as α-amyrin, β-amyrin, lupeol, and friedelin, in the leaves and/or stem bark of Korean chestnut (Castanea crenata). Triterpene biosynthesis occurs by the cyclization of 2,3-oxidosqualene to triterpenes, catalyzed by oxidosqualene cyclases (OSCs). A total of 65 putative OSC sequences were obtained from the leaf transcriptome data of C. crenata plants using PacBio sequencing. We selected 5 putative OSC unigenes, named CcOSC1-5, for functional characterization of genes involved in triterpene biosynthesis. Functional characterization of the CcOSC1-5 genes by heterologous expression in erg7 mutant yeast revealed that both CcOSC1 and CcOSC2 had a similar function, encoding multifunctional triterpene synthases producing mainly β-amyrin and a small amount of α-amyrin and lupeol. CcOSC3 encodes mixed amyrin synthase, which mainly produces β-amyrin and a small amount of α-amyrin. CcOSC4 produced mainly α-amyrin and lupeol and a small amount of β-amyrin. CcOSC5 encodes an enzyme for lupeol production as a single product. In conclusion, we identified various triterpenes and functionally characterized the triterpene synthase genes that participate in β-amyrin, α-amyrin, and lupeol biosynthesis in C. crenata.

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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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